Click here to switch to the map view.

The map label for this gene is rsmD [H]

Identifier: 170026191

GI number: 170026191

Start: 4390538

End: 4391200

Strand: Direct

Name: rsmD [H]

Synonym: YPK_3980

Alternate gene names: 170026191

Gene position: 4390538-4391200 (Clockwise)

Preceding gene: 170026186

Following gene: 170026192

Centisome position: 93.63

GC content: 50.53

Gene sequence:

>663_bases
ATGGCAAAGCAACCTATAGCAAAGCGTTCTATAGTAAAAAAAGCATCACCGCAGTCAGCGGGGCAGATAAGAATCATTGG
CGGCAAGTGGCGTGGGCGTAAACTCCCCGTTCCAGATAGCCCAGGGCTGCGCCCCACGACGGACCGGGTAAGGGAAACAT
TATTTAACTGGCTCGCTCCGATGATTCAGGGTGCTCGCTGTCTGGATTGTTTTGCGGGCAGTGGTGCGTTAGGGCTGGAA
GCACTATCCCGTTATGCAAGCGAAACTACCTTGCTGGAAGCTGATCGTCAGGTTGCCAAACAACTGAGCGACAACCTAAC
GCTACTAAAAGCTGAAAATGGGCAAGTTGTAAACACCAACTCATTACAATGGTTGGCGCAACCGGGGCAGCCTTTCAATT
TGGTATTTCTGGATCCGCCTTTTCGTAAAGGCCTACTGGCTGAAACCATTAATTTGCTAGAGCAATTTAACTGGCTGGCT
GCTGATGCCTGGATCTATGTTGAAGCGGAGGCAGAAAGCGCCGTTGCTGATGTCCCCGCCAATTGGCTGTTACATCGAGA
AAAAATCGCCGGGCAGGTTGCCTATCGTTTATTTATTCGCCAACAAACCGATTTGCCAGCATGTGCATCGGTGGCAGAGC
AGGAGCAACACCATGTGGATTAA

Upstream 100 bases:

>100_bases
GGGATATAACGTTTCCCCCACACAGCACACATCGGTAGAATAGTTGCTAATTCTTTTATTCAGCGCTGCAATAATGGCCA
ATTTTCAAGCAAAATAATCT

Downstream 100 bases:

>100_bases
TCTCGGCCGGTTACTGATGCTAGGCGTCTGGTTTTTTATACTGCTGAATCTGTTTCAGCCTTACCCTAAGCCATTGAGAT
ACTTTATCCATGTGGCCATG

Product: 16S rRNA m(2)G966-methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MAKQPIAKRSIVKKASPQSAGQIRIIGGKWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPMIQGARCLDCFAGSGALGLE
ALSRYASETTLLEADRQVAKQLSDNLTLLKAENGQVVNTNSLQWLAQPGQPFNLVFLDPPFRKGLLAETINLLEQFNWLA
ADAWIYVEAEAESAVADVPANWLLHREKIAGQVAYRLFIRQQTDLPACASVAEQEQHHVD

Sequences:

>Translated_220_residues
MAKQPIAKRSIVKKASPQSAGQIRIIGGKWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPMIQGARCLDCFAGSGALGLE
ALSRYASETTLLEADRQVAKQLSDNLTLLKAENGQVVNTNSLQWLAQPGQPFNLVFLDPPFRKGLLAETINLLEQFNWLA
ADAWIYVEAEAESAVADVPANWLLHREKIAGQVAYRLFIRQQTDLPACASVAEQEQHHVD
>Mature_219_residues
AKQPIAKRSIVKKASPQSAGQIRIIGGKWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPMIQGARCLDCFAGSGALGLEA
LSRYASETTLLEADRQVAKQLSDNLTLLKAENGQVVNTNSLQWLAQPGQPFNLVFLDPPFRKGLLAETINLLEQFNWLAA
DAWIYVEAEAESAVADVPANWLLHREKIAGQVAYRLFIRQQTDLPACASVAEQEQHHVD

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=190, Percent_Identity=70, Blast_Score=280, Evalue=4e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 24351; Mature: 24220

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQPIAKRSIVKKASPQSAGQIRIIGGKWRGRKLPVPDSPGLRPTTDRVRETLFNWLAP
CCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
MIQGARCLDCFAGSGALGLEALSRYASETTLLEADRQVAKQLSDNLTLLKAENGQVVNTN
HHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEECC
SLQWLAQPGQPFNLVFLDPPFRKGLLAETINLLEQFNWLAADAWIYVEAEAESAVADVPA
CCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHCHHEECEEEEEEECCCHHHHHCCH
NWLLHREKIAGQVAYRLFIRQQTDLPACASVAEQEQHHVD
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKQPIAKRSIVKKASPQSAGQIRIIGGKWRGRKLPVPDSPGLRPTTDRVRETLFNWLAP
CCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
MIQGARCLDCFAGSGALGLEALSRYASETTLLEADRQVAKQLSDNLTLLKAENGQVVNTN
HHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEECC
SLQWLAQPGQPFNLVFLDPPFRKGLLAETINLLEQFNWLAADAWIYVEAEAESAVADVPA
CCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHCHHEECEEEEEEECCCHHHHHCCH
NWLLHREKIAGQVAYRLFIRQQTDLPACASVAEQEQHHVD
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]