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The map label for this gene is metF [H]

Identifier: 170026097

GI number: 170026097

Start: 4284778

End: 4285578

Strand: Direct

Name: metF [H]

Synonym: YPK_3884

Alternate gene names: 170026097

Gene position: 4284778-4285578 (Clockwise)

Preceding gene: 170026096

Following gene: 170026098

Centisome position: 91.37

GC content: 35.21

Gene sequence:

>801_bases
ATGGAAAGAAAACTAAGTTTCGAAATGAATTCAGCTAAAAATGAAAAAGAAATGGAGAATATATTTAAACTTATCGAGTT
TACCGACACACTGGATCCTATTTATTACACAGTCAACACAGAAATTGGATCCTCTGTTTGGAGTGATACATATCGTACTG
TCATAGAGTTAAGAGAAAAAACAGATGTTCCCCTGATACCTCACATTACAATAAATAATAAAAAAGAAAGGGAGATAATG
CAGATTATAGAAAAATATATAAACGTAGGCATTTGTGAGTTCTTCGTGATTCGTGGTGACAAGCATGTGTACGACCAAGA
TAACCCCATTAATTACGGGGTTGAATTGATTGAGCTGATACGCCGTAGCCACAAACATATCGGAATCAAAACCAGCCTGT
ACCCTGACTTTCACAAGGAAACCACAGAGGTAAAAGAAGAAATAGACTGGAATGAAAGAAAATATCAACTCGGGGTTAAT
GAGTTTATCAGCCAACTGACACTCAACACGAATGCATTGGATTTCCTGAGTCACTGGGTTGATAAAGAACGACCCTTTAC
GCCATCCATAATGCCACTTGGCAATTTTTCATTTATTGAAAAATTTACTTCATCTAACTCAATCGACTACCCTTTGTGGA
TTAAAAAATTCGCAAATAGTAAGAGCAATAGTAATGAAGATAAAGAAAACATCGGGATTGAGATAATTCGCTTTCTCTGT
AGAGAATATCTCAATCAAAGGGAGAGGCTTCATGTATTCACTTTGAATAAAGTTGATGTTATGAAGAAAATTTTCCAATA
A

Upstream 100 bases:

>100_bases
GACGATATTTAATTTGCATTCAACAATAAATAAATGTTATTTAAATTATTCGTGATTGCGTTAATTAAACAGATTATACC
CACACAAAGAAGAGAACATT

Downstream 100 bases:

>100_bases
GGAATTGTACACCGAAAGATTCTGTTGCATTAGCGCAGAACTGAGACTGTCATTAAAATTGTGATATTGCCTGATGGCTA
TGATACACCAAAATAACCTC

Product: methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MERKLSFEMNSAKNEKEMENIFKLIEFTDTLDPIYYTVNTEIGSSVWSDTYRTVIELREKTDVPLIPHITINNKKEREIM
QIIEKYINVGICEFFVIRGDKHVYDQDNPINYGVELIELIRRSHKHIGIKTSLYPDFHKETTEVKEEIDWNERKYQLGVN
EFISQLTLNTNALDFLSHWVDKERPFTPSIMPLGNFSFIEKFTSSNSIDYPLWIKKFANSKSNSNEDKENIGIEIIRFLC
REYLNQRERLHVFTLNKVDVMKKIFQ

Sequences:

>Translated_266_residues
MERKLSFEMNSAKNEKEMENIFKLIEFTDTLDPIYYTVNTEIGSSVWSDTYRTVIELREKTDVPLIPHITINNKKEREIM
QIIEKYINVGICEFFVIRGDKHVYDQDNPINYGVELIELIRRSHKHIGIKTSLYPDFHKETTEVKEEIDWNERKYQLGVN
EFISQLTLNTNALDFLSHWVDKERPFTPSIMPLGNFSFIEKFTSSNSIDYPLWIKKFANSKSNSNEDKENIGIEIIRFLC
REYLNQRERLHVFTLNKVDVMKKIFQ
>Mature_266_residues
MERKLSFEMNSAKNEKEMENIFKLIEFTDTLDPIYYTVNTEIGSSVWSDTYRTVIELREKTDVPLIPHITINNKKEREIM
QIIEKYINVGICEFFVIRGDKHVYDQDNPINYGVELIELIRRSHKHIGIKTSLYPDFHKETTEVKEEIDWNERKYQLGVN
EFISQLTLNTNALDFLSHWVDKERPFTPSIMPLGNFSFIEKFTSSNSIDYPLWIKKFANSKSNSNEDKENIGIEIIRFLC
REYLNQRERLHVFTLNKVDVMKKIFQ

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Escherichia coli, GI1790377, Length=269, Percent_Identity=23.0483271375465, Blast_Score=83, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325234, Length=282, Percent_Identity=21.2765957446809, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 31648; Mature: 31648

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERKLSFEMNSAKNEKEMENIFKLIEFTDTLDPIYYTVNTEIGSSVWSDTYRTVIELREK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHH
TDVPLIPHITINNKKEREIMQIIEKYINVGICEFFVIRGDKHVYDQDNPINYGVELIELI
CCCCCCCEEEECCHHHHHHHHHHHHHHCCCHHHEEEEECCCEECCCCCCHHHHHHHHHHH
RRSHKHIGIKTSLYPDFHKETTEVKEEIDWNERKYQLGVNEFISQLTLNTNALDFLSHWV
HHHHHHCCCEECCCCCHHHHHHHHHHHCCCCHHHEECCHHHHHHHHHCCCHHHHHHHHHH
DKERPFTPSIMPLGNFSFIEKFTSSNSIDYPLWIKKFANSKSNSNEDKENIGIEIIRFLC
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
REYLNQRERLHVFTLNKVDVMKKIFQ
HHHHHHHHEEEEEECCHHHHHHHHHC
>Mature Secondary Structure
MERKLSFEMNSAKNEKEMENIFKLIEFTDTLDPIYYTVNTEIGSSVWSDTYRTVIELREK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHH
TDVPLIPHITINNKKEREIMQIIEKYINVGICEFFVIRGDKHVYDQDNPINYGVELIELI
CCCCCCCEEEECCHHHHHHHHHHHHHHCCCHHHEEEEECCCEECCCCCCHHHHHHHHHHH
RRSHKHIGIKTSLYPDFHKETTEVKEEIDWNERKYQLGVNEFISQLTLNTNALDFLSHWV
HHHHHHCCCEECCCCCHHHHHHHHHHHCCCCHHHEECCHHHHHHHHHCCCHHHHHHHHHH
DKERPFTPSIMPLGNFSFIEKFTSSNSIDYPLWIKKFANSKSNSNEDKENIGIEIIRFLC
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
REYLNQRERLHVFTLNKVDVMKKIFQ
HHHHHHHHEEEEEECCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]