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The map label for this gene is thiI [H]

Identifier: 170025467

GI number: 170025467

Start: 3553868

End: 3555319

Strand: Reverse

Name: thiI [H]

Synonym: YPK_3250

Alternate gene names: 170025467

Gene position: 3555319-3553868 (Counterclockwise)

Preceding gene: 170025471

Following gene: 170025464

Centisome position: 75.82

GC content: 47.11

Gene sequence:

>1452_bases
ATGAAGTTTATCATTAAATTGTTCCCAGAAATCACCATCAAGAGTCAATCTGTGCGATTGCGCTTCATTAAGATCCTGAC
TACCAATATCCGCAACGTACTGAAACACCTTGAGGACGATACCCTCGCCATTGTTCGTCATTGGGATCATATCGAGCTTC
GTACCAAAGATGACAATCTTGGCCCGGAGATTTGTGATGCACTGACCCGCATACCGGGTATTCACCATATCCTTGAAGTA
GAAGATCGTAGCTACAGCGATATGCACAATATTTTTGAACAAACGCTGGAAGCCTACCGGGAGACACTGGTTGGGAAGAC
TTTCTGTGTTCGGGTAAAACGTCGTGGCAAGCATGAATTCTCTTCTGGTGATGTTGAACGATATGTCGGTGGGGGTCTGA
ATCAACATATTGAAAGTGCTAAAGTAAACCTGACCCGCCCACAGGTGACGGTTAATTTGGAAGTTGATCAAGACAAACTG
ATCTTGGTTAAGGCACGTCATGAAGGGCTGGGGGGCTTCCCTATCGGCACTCAGGAGGATGTTCTCTCCCTGATTTCAGG
CGGTTTTGATTCAGGTGTATCGAGCTATATGCTGATGCGCCGTGGCTGTCGTGTCCATTATTGTTTCTTTAATCTCGGTG
GCTCAGCCCATGAGATAGGTGTTAAGCAGGTGGCACATTATCTGTGGAATCGCTTTGGCAGCTCCCATCGAGTACGGTTT
ATCGCCATTGATTTTGAACCGGTTGTTGGCGAGATCCTGGAAAAAGTTGAAGATGGCCAGATGGGCGTAGTATTGAAGCG
CATGATGGTGCGCGCAGCTTCTCAGGTTGCTGAACGCTATGGTGTTCAGGCTTTAGTTACCGGTGAGGCGCTGGGGCAGG
TATCCAGCCAGACACTGACTAACCTGCGTTTAATTGATAATGCCTCGGATACCCTAATTTTGCGCCCGCTTATCTCCCAC
GATAAAGAGCACATCATTAATTTGGCACGGCAGATTGGTACGGAAGATTTTGCTAAAACCATGCCGGAATATTGTGGCGT
TATTTCAAAAAGCCCAACGGTAAAAGCGGTCAAAGCTAAAATCGAAGAAGAGGAGTCTCACTTTGATTTCTCTATTCTGG
ATCGGGTTGTGAGCGAAGCTAAAAATGTTGATATCCGTGAAATCGCACAGCAAAGCCGTGAACAAGTCGTTGAAGTTGAA
ACCGTTGCTGAATTGGCCGATACCGATGTGTTGCTAGATATTCGTGCGCCTGATGAACAGGAAGAGAAGCCACTGAAACT
GGATCAGGTTGAAGTGCGATCACTGCCGTTCTATAAGCTCAGCTCGCAATTTGCCGATCTGGACCAGAGTAAAACCTATT
TGCTGTATTGTGATCGCGGTGTGATGAGCCGTTTGCAGGCCCTTTATTTGCGTGAGCAGGGGTATACCAACGTGAAAGTC
TATCGCCCTTAG

Upstream 100 bases:

>100_bases
TAATGATATACTCCGCGGCTTGCATATTTTCGCTAACGTTTATGGCCGTTGCTTTCAATAGCAACGCTATCATTCCATCA
CTAGACAACTAAAAGCCGCC

Downstream 100 bases:

>100_bases
TTTTTTGATAATTGACGGCACTGCGCGTTGCCATTCAGGATTTGCGCAGTAACCGTTACGGCGGCCATCATTCCCGATAA
TTATAAATTCCTGGCGGCAA

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MKFIIKLFPEITIKSQSVRLRFIKILTTNIRNVLKHLEDDTLAIVRHWDHIELRTKDDNLGPEICDALTRIPGIHHILEV
EDRSYSDMHNIFEQTLEAYRETLVGKTFCVRVKRRGKHEFSSGDVERYVGGGLNQHIESAKVNLTRPQVTVNLEVDQDKL
ILVKARHEGLGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGSAHEIGVKQVAHYLWNRFGSSHRVRF
IAIDFEPVVGEILEKVEDGQMGVVLKRMMVRAASQVAERYGVQALVTGEALGQVSSQTLTNLRLIDNASDTLILRPLISH
DKEHIINLARQIGTEDFAKTMPEYCGVISKSPTVKAVKAKIEEEESHFDFSILDRVVSEAKNVDIREIAQQSREQVVEVE
TVAELADTDVLLDIRAPDEQEEKPLKLDQVEVRSLPFYKLSSQFADLDQSKTYLLYCDRGVMSRLQALYLREQGYTNVKV
YRP

Sequences:

>Translated_483_residues
MKFIIKLFPEITIKSQSVRLRFIKILTTNIRNVLKHLEDDTLAIVRHWDHIELRTKDDNLGPEICDALTRIPGIHHILEV
EDRSYSDMHNIFEQTLEAYRETLVGKTFCVRVKRRGKHEFSSGDVERYVGGGLNQHIESAKVNLTRPQVTVNLEVDQDKL
ILVKARHEGLGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGSAHEIGVKQVAHYLWNRFGSSHRVRF
IAIDFEPVVGEILEKVEDGQMGVVLKRMMVRAASQVAERYGVQALVTGEALGQVSSQTLTNLRLIDNASDTLILRPLISH
DKEHIINLARQIGTEDFAKTMPEYCGVISKSPTVKAVKAKIEEEESHFDFSILDRVVSEAKNVDIREIAQQSREQVVEVE
TVAELADTDVLLDIRAPDEQEEKPLKLDQVEVRSLPFYKLSSQFADLDQSKTYLLYCDRGVMSRLQALYLREQGYTNVKV
YRP
>Mature_483_residues
MKFIIKLFPEITIKSQSVRLRFIKILTTNIRNVLKHLEDDTLAIVRHWDHIELRTKDDNLGPEICDALTRIPGIHHILEV
EDRSYSDMHNIFEQTLEAYRETLVGKTFCVRVKRRGKHEFSSGDVERYVGGGLNQHIESAKVNLTRPQVTVNLEVDQDKL
ILVKARHEGLGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGSAHEIGVKQVAHYLWNRFGSSHRVRF
IAIDFEPVVGEILEKVEDGQMGVVLKRMMVRAASQVAERYGVQALVTGEALGQVSSQTLTNLRLIDNASDTLILRPLISH
DKEHIINLARQIGTEDFAKTMPEYCGVISKSPTVKAVKAKIEEEESHFDFSILDRVVSEAKNVDIREIAQQSREQVVEVE
TVAELADTDVLLDIRAPDEQEEKPLKLDQVEVRSLPFYKLSSQFADLDQSKTYLLYCDRGVMSRLQALYLREQGYTNVKV
YRP

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=483, Percent_Identity=81.7805383022774, Blast_Score=800, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 54895; Mature: 54895

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS51165 THUMP ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIIKLFPEITIKSQSVRLRFIKILTTNIRNVLKHLEDDTLAIVRHWDHIELRTKDDNL
CCCCEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCC
GPEICDALTRIPGIHHILEVEDRSYSDMHNIFEQTLEAYRETLVGKTFCVRVKRRGKHEF
CHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCC
SSGDVERYVGGGLNQHIESAKVNLTRPQVTVNLEVDQDKLILVKARHEGLGGFPIGTQED
CCCCHHHHHCCCHHHHHHHCEEEEECCEEEEEEEECCCCEEEEEECCCCCCCCCCCCHHH
VLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGSAHEIGVKQVAHYLWNRFGSSHRVRF
HHHHHHCCCCHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEE
IAIDFEPVVGEILEKVEDGQMGVVLKRMMVRAASQVAERYGVQALVTGEALGQVSSQTLT
EEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHH
NLRLIDNASDTLILRPLISHDKEHIINLARQIGTEDFAKTMPEYCGVISKSPTVKAVKAK
HEEEECCCCCCEEECHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHH
IEEEESHFDFSILDRVVSEAKNVDIREIAQQSREQVVEVETVAELADTDVLLDIRAPDEQ
HHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
EEKPLKLDQVEVRSLPFYKLSSQFADLDQSKTYLLYCDRGVMSRLQALYLREQGYTNVKV
CCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEE
YRP
ECC
>Mature Secondary Structure
MKFIIKLFPEITIKSQSVRLRFIKILTTNIRNVLKHLEDDTLAIVRHWDHIELRTKDDNL
CCCCEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCC
GPEICDALTRIPGIHHILEVEDRSYSDMHNIFEQTLEAYRETLVGKTFCVRVKRRGKHEF
CHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCC
SSGDVERYVGGGLNQHIESAKVNLTRPQVTVNLEVDQDKLILVKARHEGLGGFPIGTQED
CCCCHHHHHCCCHHHHHHHCEEEEECCEEEEEEEECCCCEEEEEECCCCCCCCCCCCHHH
VLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGSAHEIGVKQVAHYLWNRFGSSHRVRF
HHHHHHCCCCHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEE
IAIDFEPVVGEILEKVEDGQMGVVLKRMMVRAASQVAERYGVQALVTGEALGQVSSQTLT
EEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHH
NLRLIDNASDTLILRPLISHDKEHIINLARQIGTEDFAKTMPEYCGVISKSPTVKAVKAK
HEEEECCCCCCEEECHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHH
IEEEESHFDFSILDRVVSEAKNVDIREIAQQSREQVVEVETVAELADTDVLLDIRAPDEQ
HHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
EEKPLKLDQVEVRSLPFYKLSSQFADLDQSKTYLLYCDRGVMSRLQALYLREQGYTNVKV
CCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEE
YRP
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA