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The map label for this gene is cof

Identifier: 170025442

GI number: 170025442

Start: 3526034

End: 3526855

Strand: Reverse

Name: cof

Synonym: YPK_3225

Alternate gene names: 170025442

Gene position: 3526855-3526034 (Counterclockwise)

Preceding gene: 170025445

Following gene: 170025440

Centisome position: 75.21

GC content: 47.08

Gene sequence:

>822_bases
ATGTACCGTTTAGCCGCTTTTGATATGGATGGAACGTTGCTGATGCGTGACCATAAAATAGGTAGCATCACACTGAATGC
GCTGCATCAATTAGCCGATGCTGGGGTAACTCTGACCTTCGCGACCGGGCGGCACTACCTTGATATGAAAGGCATATTAT
CCCATTCAGGCCTAAATGGTTATTTGATTACCGGTAATGGCACCCGAGTCTGCGATGCTGAAGGTAATCCTTTATACGGC
ATGGACTTACCGGCAGAGTTGGTGGAATTTGTGCTGCGTACCCCATGGCAGACAAATGCCAGTATTCACCTTTTCCGTGA
TGATGGCTGGTTTACTGATCGTAATGATCCCGATCTGCTGATAGCACACACTACCAGTGGTTTCCATTTCCAGCTTACCG
AATGGGATGAGTTGCCTCTAACCGGTAATCACAAGTTCTGTTTTATTGCTTCACATCAGGAACTTGTCGAATTGAAAGCG
CAATTAGAGCAGCAGATGAGCGGTGAAGCTGATTTCTGTTTTTCAGCGACGGATTGTCTTGAAGTTTTACCCCGTGGTTG
TAACAAAGGGGTGGCTTTAGAAAAACTAAGCCACCATCTTGATTTAACCTTGGCTGACTGTATGGCATTCGGCGACGCGA
TGAATGATAAAGAAATGTTGTCGCGCGTGGGGCGTGGGTTAGTGATGGGCAATGCATTACCGCAATTGAAGCAAGAATTA
CCCCAGTTACAGATTATTGGTCGCTGTGAGCAACAAGGCGTCGCCCACTATTTGCACCATTGGCTAAGTTCACCACACCT
CACCTATTCCCCCGAATTCTGA

Upstream 100 bases:

>100_bases
AAATCCGGAATAGTTTAAATTATTTGTTCACTATTAGTTCCGTAAATACTACGCCATAATTTTATCCATGCCACCGGTTT
CATATTTTACGAGGTTTTGA

Downstream 100 bases:

>100_bases
GGTGATAGCTATTAGCCAGCCGGAGAACCATCCGGTTTTTTTCTTCCGTAGGCGGTTTGTCAGTGTTCATGCCTAGCTGA
CAAACCGCTTTTATCTCTTT

Product: Cof-like hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MYRLAAFDMDGTLLMRDHKIGSITLNALHQLADAGVTLTFATGRHYLDMKGILSHSGLNGYLITGNGTRVCDAEGNPLYG
MDLPAELVEFVLRTPWQTNASIHLFRDDGWFTDRNDPDLLIAHTTSGFHFQLTEWDELPLTGNHKFCFIASHQELVELKA
QLEQQMSGEADFCFSATDCLEVLPRGCNKGVALEKLSHHLDLTLADCMAFGDAMNDKEMLSRVGRGLVMGNALPQLKQEL
PQLQIIGRCEQQGVAHYLHHWLSSPHLTYSPEF

Sequences:

>Translated_273_residues
MYRLAAFDMDGTLLMRDHKIGSITLNALHQLADAGVTLTFATGRHYLDMKGILSHSGLNGYLITGNGTRVCDAEGNPLYG
MDLPAELVEFVLRTPWQTNASIHLFRDDGWFTDRNDPDLLIAHTTSGFHFQLTEWDELPLTGNHKFCFIASHQELVELKA
QLEQQMSGEADFCFSATDCLEVLPRGCNKGVALEKLSHHLDLTLADCMAFGDAMNDKEMLSRVGRGLVMGNALPQLKQEL
PQLQIIGRCEQQGVAHYLHHWLSSPHLTYSPEF
>Mature_273_residues
MYRLAAFDMDGTLLMRDHKIGSITLNALHQLADAGVTLTFATGRHYLDMKGILSHSGLNGYLITGNGTRVCDAEGNPLYG
MDLPAELVEFVLRTPWQTNASIHLFRDDGWFTDRNDPDLLIAHTTSGFHFQLTEWDELPLTGNHKFCFIASHQELVELKA
QLEQQMSGEADFCFSATDCLEVLPRGCNKGVALEKLSHHLDLTLADCMAFGDAMNDKEMLSRVGRGLVMGNALPQLKQEL
PQLQIIGRCEQQGVAHYLHHWLSSPHLTYSPEF

Specific function: Catalyzes the hydrolysis of 4-amino-2-methyl-5- hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2- methyl-5-hydroxymethylpyrimidine phosphate (HMP-P)

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family

Homologues:

Organism=Escherichia coli, GI87081741, Length=272, Percent_Identity=57.3529411764706, Blast_Score=317, Evalue=5e-88,
Organism=Escherichia coli, GI48994981, Length=263, Percent_Identity=36.5019011406844, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI2367265, Length=243, Percent_Identity=27.1604938271605, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COF_YERP3 (A7FLB5)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001402043.1
- ProteinModelPortal:   A7FLB5
- GeneID:   5385935
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_3083
- NMPDR:   fig|349747.3.peg.3623
- eggNOG:   COG0561
- HOGENOM:   HBG728500
- OMA:   NECIPEV
- ProtClustDB:   PRK15126
- BioCyc:   YPSE349747:YPSIP31758_3083-MONOMER
- HAMAP:   MF_01847
- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150
- Gene3D:   G3DSA:3.40.50.1000
- TIGRFAMs:   TIGR00099
- TIGRFAMs:   TIGR01484

Pfam domain/function: PF08282 Hydrolase_3; SSF56784 SSF56784

EC number: NA

Molecular weight: Translated: 30512; Mature: 30512

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS01228 COF_1; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRLAAFDMDGTLLMRDHKIGSITLNALHQLADAGVTLTFATGRHYLDMKGILSHSGLNG
CEEEEEECCCCEEEEECCCCCEEEHHHHHHHHHCCEEEEEECCCEEEEHHHHHHCCCCCE
YLITGNGTRVCDAEGNPLYGMDLPAELVEFVLRTPWQTNASIHLFRDDGWFTDRNDPDLL
EEEECCCCEEECCCCCEEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCEECCCCCCCEE
IAHTTSGFHFQLTEWDELPLTGNHKFCFIASHQELVELKAQLEQQMSGEADFCFSATDCL
EEEECCCEEEEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCEEECHHHHH
EVLPRGCNKGVALEKLSHHLDLTLADCMAFGDAMNDKEMLSRVGRGLVMGNALPQLKQEL
HHHHCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCHHHHHHC
PQLQIIGRCEQQGVAHYLHHWLSSPHLTYSPEF
CCEEEHHHHHHHHHHHHHHHHHCCCCEEECCCC
>Mature Secondary Structure
MYRLAAFDMDGTLLMRDHKIGSITLNALHQLADAGVTLTFATGRHYLDMKGILSHSGLNG
CEEEEEECCCCEEEEECCCCCEEEHHHHHHHHHCCEEEEEECCCEEEEHHHHHHCCCCCE
YLITGNGTRVCDAEGNPLYGMDLPAELVEFVLRTPWQTNASIHLFRDDGWFTDRNDPDLL
EEEECCCCEEECCCCCEEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCEECCCCCCCEE
IAHTTSGFHFQLTEWDELPLTGNHKFCFIASHQELVELKAQLEQQMSGEADFCFSATDCL
EEEECCCEEEEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCEEECHHHHH
EVLPRGCNKGVALEKLSHHLDLTLADCMAFGDAMNDKEMLSRVGRGLVMGNALPQLKQEL
HHHHCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCHHHHHHC
PQLQIIGRCEQQGVAHYLHHWLSSPHLTYSPEF
CCEEEHHHHHHHHHHHHHHHHHCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA