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The map label for this gene is fsr [H]

Identifier: 170025391

GI number: 170025391

Start: 3468433

End: 3469212

Strand: Direct

Name: fsr [H]

Synonym: YPK_3174

Alternate gene names: 170025391

Gene position: 3468433-3469212 (Clockwise)

Preceding gene: 170025390

Following gene: 170025392

Centisome position: 73.96

GC content: 43.59

Gene sequence:

>780_bases
TTGGCTCAATCTGTGTTTCAGGTGGGGGGGAATTTTGGCAGCGCACTCGGTCCGTTACTGGCCGCCATCATTATTGCCCC
TTACGGTAAAGGCAATGTGGGCTGGTTTTCGCTCGCAGCCCTACTGGCAATTGTCGTGCTGTTGCAGGTGAGTAAATGGT
ACAAACTGCAGCAACGTGCTTCGTATGGCAAAGTGTTAAAAATCTCACCAGCCAAAATACTAACAAAAAATAAAATTATC
AGTACTTTAGCCATCCTGATGGTGCTGATATTCTCTAAATACTTCTATTTGACCAGCATTAGTAGCTATTACACCTTTTA
TTTAATACATAAGTTTGGCGTTTCGGTACAAAGTGCCCAGATACACCTATTTGTTTTCTTATTTGCGGTTGCCGCCGGCA
CCATTATTGGTGGGCCTCTTGGTGATAAGATTGGTAGAAAATATGTTATTTGGGGTTCTATACTCGGTGTTGCCCCTTTT
ACCCTTGCTTTACCCTACGCGTCTTTGTATTGGACGGGTATTTTAACCGTATTTATTGGTGTTATTCTCGCTTCCGCCTT
TTCAGCAATACTGGTGTATGCTCAAGAGCTTATCCCAGGGAAAGTCGGCATGGTATCGGGTTTATTCTTCGGTTTCGCTT
TCGGTATGGGGGGAATAGGTGCAGCAGTACTGGGGTATGTTGCTGATTTAACCAGTATTGAACTGGTTTATCAAATATGT
GCGTTCTTGCCATTACTGGGAATATTCACGGCCTTACTGCCTAATTTGGACGATAAGTAA

Upstream 100 bases:

>100_bases
TGCAAATGTCAGATCGCGATGCGCCTGTTAGGGCGCGGCTGGTAACAGAGCCTTATAGGCGCATATGAAAAACCTCCGGC
TATGCCGGAGGATATTTATT

Downstream 100 bases:

>100_bases
TAGTAATAGCGCTATCGCCCTGACTGAAATGTCTATTTTGGTCAGGAGCCTCCCCTATGTAAGCTCGATATAAATAGCAA
TTCCTCCACAAACAACCACT

Product: putative membrane efflux protein

Products: fosmidomycin [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MAQSVFQVGGNFGSALGPLLAAIIIAPYGKGNVGWFSLAALLAIVVLLQVSKWYKLQQRASYGKVLKISPAKILTKNKII
STLAILMVLIFSKYFYLTSISSYYTFYLIHKFGVSVQSAQIHLFVFLFAVAAGTIIGGPLGDKIGRKYVIWGSILGVAPF
TLALPYASLYWTGILTVFIGVILASAFSAILVYAQELIPGKVGMVSGLFFGFAFGMGGIGAAVLGYVADLTSIELVYQIC
AFLPLLGIFTALLPNLDDK

Sequences:

>Translated_259_residues
MAQSVFQVGGNFGSALGPLLAAIIIAPYGKGNVGWFSLAALLAIVVLLQVSKWYKLQQRASYGKVLKISPAKILTKNKII
STLAILMVLIFSKYFYLTSISSYYTFYLIHKFGVSVQSAQIHLFVFLFAVAAGTIIGGPLGDKIGRKYVIWGSILGVAPF
TLALPYASLYWTGILTVFIGVILASAFSAILVYAQELIPGKVGMVSGLFFGFAFGMGGIGAAVLGYVADLTSIELVYQIC
AFLPLLGIFTALLPNLDDK
>Mature_258_residues
AQSVFQVGGNFGSALGPLLAAIIIAPYGKGNVGWFSLAALLAIVVLLQVSKWYKLQQRASYGKVLKISPAKILTKNKIIS
TLAILMVLIFSKYFYLTSISSYYTFYLIHKFGVSVQSAQIHLFVFLFAVAAGTIIGGPLGDKIGRKYVIWGSILGVAPFT
LALPYASLYWTGILTVFIGVILASAFSAILVYAQELIPGKVGMVSGLFFGFAFGMGGIGAAVLGYVADLTSIELVYQICA
FLPLLGIFTALLPNLDDK

Specific function: Confers the resistance against fosmidomycin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786686, Length=259, Percent_Identity=74.9034749034749, Blast_Score=358, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 27732; Mature: 27600

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AQSVFQVGGNFGSALGPLLAAIIIAPYGKGNVGWFSLAALLAIVVLLQVSKWYKLQQRA
CCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYGKVLKISPAKILTKNKIISTLAILMVLIFSKYFYLTSISSYYTFYLIHKFGVSVQSAQ
CCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IHLFVFLFAVAAGTIIGGPLGDKIGRKYVIWGSILGVAPFTLALPYASLYWTGILTVFIG
HHHHHHHHHHHHHHHCCCCCHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VILASAFSAILVYAQELIPGKVGMVSGLFFGFAFGMGGIGAAVLGYVADLTSIELVYQIC
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AFLPLLGIFTALLPNLDDK
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: fosmidomycin [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: fosmidomycin [Cytoplasm] + Proton [Periplasm] = fosmidomycin [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8917080; 9278503 [H]