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The map label for this gene is cueR

Identifier: 170025383

GI number: 170025383

Start: 3459335

End: 3459754

Strand: Reverse

Name: cueR

Synonym: YPK_3164

Alternate gene names: 170025383

Gene position: 3459754-3459335 (Counterclockwise)

Preceding gene: 170025393

Following gene: 170025377

Centisome position: 73.78

GC content: 46.67

Gene sequence:

>420_bases
ATGAACATTAGCGATGTTGCGAAAAAAACCGGTTTAACCAGCAAAGCCATTCGTTTTTATGAAGAAAAAAAACTGGTGAC
GCCACCTGTTCGCACGGATAACGGTTATCGCAGCTACACGGCCAAACATATTGAAGAGCTCACGTTGCTGCGCCAGGCGC
GGCAAGTGGGTTTTACACTGGATGAGTGTCGTGAATTGCTGGCATTATTTCATAACCCAGCACGGCACAGCGCGGATGTG
AAAGCGGCAACATTGCTAAAAGTAGCGGAAATCGAGCAACATATTAATGATTTGAATCAAATGCGTATGCGTTTGTTAGC
GTTGGCAGAAGAGTGCCCAGGGGATGAGGGCGCAGATTGCCCAATTATTAATAGCCTGGCGGGATGTTGCCACTCAAGCA
GTTCGCTACCATTAAAATAA

Upstream 100 bases:

>100_bases
TAATATCGGGGGGCGGCTTTTATTCCGCTTGACTGGCATGGCTATTTTCACTAACCATAGAGGTTAAACCTTCCAGCAAG
GTGAAGGTCAAGGGGGAATA

Downstream 100 bases:

>100_bases
TACGCTGCCGTGAAAATAATACGTTGCCATTAAAGTCATGACTCGCCATCCGAGCAATAAGCCGCATCTTTAACGCTGCC
GTGATGAATAACCCGAATCA

Product: MerR family transcriptional regulator

Products: NA

Alternate protein names: Copper efflux regulator; Copper export regulator

Number of amino acids: Translated: 139; Mature: 139

Protein sequence:

>139_residues
MNISDVAKKTGLTSKAIRFYEEKKLVTPPVRTDNGYRSYTAKHIEELTLLRQARQVGFTLDECRELLALFHNPARHSADV
KAATLLKVAEIEQHINDLNQMRMRLLALAEECPGDEGADCPIINSLAGCCHSSSSLPLK

Sequences:

>Translated_139_residues
MNISDVAKKTGLTSKAIRFYEEKKLVTPPVRTDNGYRSYTAKHIEELTLLRQARQVGFTLDECRELLALFHNPARHSADV
KAATLLKVAEIEQHINDLNQMRMRLLALAEECPGDEGADCPIINSLAGCCHSSSSLPLK
>Mature_139_residues
MNISDVAKKTGLTSKAIRFYEEKKLVTPPVRTDNGYRSYTAKHIEELTLLRQARQVGFTLDECRELLALFHNPARHSADV
KAATLLKVAEIEQHINDLNQMRMRLLALAEECPGDEGADCPIINSLAGCCHSSSSLPLK

Specific function: Regulates the transcription of the copA and cueO genes. It detects cytoplasmic copper stress and activates transcription in response to increasing copper concentrations

COG id: COG0789

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH merR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1786695, Length=131, Percent_Identity=72.5190839694656, Blast_Score=201, Evalue=1e-53,
Organism=Escherichia coli, GI1789687, Length=128, Percent_Identity=32.8125, Blast_Score=79, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CUER_YERPE (Q8ZCA8)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AD0375
- RefSeq:   NP_668421.1
- RefSeq:   NP_992221.1
- RefSeq:   YP_002348005.1
- ProteinModelPortal:   Q8ZCA8
- SMR:   Q8ZCA8
- GeneID:   1146041
- GeneID:   1175906
- GeneID:   2764733
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO3085
- KEGG:   ypk:y1094
- KEGG:   ypm:YP_0839
- HOGENOM:   HBG710951
- OMA:   TRTHLAY
- ProtClustDB:   CLSK889409
- BioCyc:   YPES187410:Y1094-MONOMER
- BioCyc:   YPES214092:YPO3085-MONOMER
- GO:   GO:0005737
- InterPro:   IPR011789
- InterPro:   IPR009061
- InterPro:   IPR000551
- InterPro:   IPR015358
- PRINTS:   PR00040
- SMART:   SM00422
- TIGRFAMs:   TIGR02044

Pfam domain/function: PF00376 MerR; PF09278 MerR-DNA-bind; SSF46955 Putativ_DNA_bind

EC number: NA

Molecular weight: Translated: 15463; Mature: 15463

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS00552 HTH_MERR_1; PS50937 HTH_MERR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISDVAKKTGLTSKAIRFYEEKKLVTPPVRTDNGYRSYTAKHIEELTLLRQARQVGFTL
CCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
DECRELLALFHNPARHSADVKAATLLKVAEIEQHINDLNQMRMRLLALAEECPGDEGADC
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PIINSLAGCCHSSSSLPLK
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNISDVAKKTGLTSKAIRFYEEKKLVTPPVRTDNGYRSYTAKHIEELTLLRQARQVGFTL
CCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
DECRELLALFHNPARHSADVKAATLLKVAEIEQHINDLNQMRMRLLALAEECPGDEGADC
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PIINSLAGCCHSSSSLPLK
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430