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The map label for this gene is intS [H]

Identifier: 170025368

GI number: 170025368

Start: 3443988

End: 3445196

Strand: Reverse

Name: intS [H]

Synonym: YPK_3149

Alternate gene names: 170025368

Gene position: 3445196-3443988 (Counterclockwise)

Preceding gene: 170025371

Following gene: 170025361

Centisome position: 73.47

GC content: 47.89

Gene sequence:

>1209_bases
ATGGCATTAACCGATGTGAAGGTAAGAAGTGCAAAACCTACTGACAAGCCTTATAAGCTTACCGATGGCGAAGGCATGCA
TTTAATGGTTCATCCCAATGGATCTAAATACTGGCGCTTACAGTACCGCTTTGACGGTAAGCAAAAGATGTTGGCACTGG
GTATCTACCCTGAGATTTCGCTATCTGAAGCCAGGCAGCGGAGAGATGAAGCTAAGCGACAAGTCGCTAACGCTATCGAT
CCCAGCGAACAGAAAAAGGTTGAGAAACAAGCTCGCAAAGCGACAGTGGAAAATACCTTTAAAGCTATTGCTCTGGAATG
GCATGAGTACAAACGGCCAAACTGGTCCAAAGGCTACGCGGAAGACATCATGGAAGCGTTTGAAAACGATATCTTCCCGG
ATATTGGTAAGCGCCCAATAGCAGAGATAAAGCCTCTGGAGATCCTCAGTTCACTTCGTAAACTCGAAAAACGTGGCGTC
CTCGACAAGTTGCGCAAAATCCGCCAGGCCTGCAACCAAGTGTTTCGTTACGCCATTGTCACTGGTCGCGCTGAATCTAA
CCCTGCTTCAGAACTGGCAAGTGCACTAACTCCGCCAAAATCTGTGCACTATCCTCACCTGTTAGCGGATGAACTTCCAG
CTTTCTTAGAGGCTCTCGCCGCTTATTCTGGTAGCCCAATAACTCGGCTAGCAACAAAGATTCTGATGCTAACTGGTGTT
CGTACCATTGAGCTCCGCATGGCTGAATGGAAAGAGTTCGATTTCGACCAGCGGGTTTGGGAAGTACCGGTACAAAGGAT
GAAGATGCGGCGACCTCACCTTGTACCACTATCTGATCAAGTTGTGACAGCGCTACGTGAAATTCAGGCGGTAACTGGCC
GTTATAACTTGGTGTTTCCTGGACGTAACGACATCACCAAACCAATGAGCGAAGCGAGTATCAATCAGGTACTGAAAAGG
ATTGGGTATCACGGGAAGGCGACAGGTCATGGCTTCAGGCACACGATGAGTACTATCTTGCATGAACAAGGCTATAACAC
GGCTTGGATCGAGTTACAGCTGGCTCATGTGGATAAGAATACCATTCGTGGCACATACAACCATGCGCAGTATCTGGAGC
AACGTCGAGAGATGTTGCAGTGGTATGGGGAATATGTGGATGGGTTGGCGGCTAGGGGAATCGCAATAAATATTCGTAAA
AAAGCATAA

Upstream 100 bases:

>100_bases
CCTACATCTATCAGCATCAAGTAGTATCTGTTGGTATATGTGATGGTACATCCCCGTTCAATGAAATCTTGTACCAACAG
CCAGCAACGGAGGGCCTCAA

Downstream 100 bases:

>100_bases
ATGTTTTGCTGTTCCAGTATGAGCATCTACCGCCATAAGCAATGTTTTACTCGGTGCGAACTGAGGCAAAGCCTGCTCAT
TTGGGTAAGAACGCGATATG

Product: integrase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MALTDVKVRSAKPTDKPYKLTDGEGMHLMVHPNGSKYWRLQYRFDGKQKMLALGIYPEISLSEARQRRDEAKRQVANAID
PSEQKKVEKQARKATVENTFKAIALEWHEYKRPNWSKGYAEDIMEAFENDIFPDIGKRPIAEIKPLEILSSLRKLEKRGV
LDKLRKIRQACNQVFRYAIVTGRAESNPASELASALTPPKSVHYPHLLADELPAFLEALAAYSGSPITRLATKILMLTGV
RTIELRMAEWKEFDFDQRVWEVPVQRMKMRRPHLVPLSDQVVTALREIQAVTGRYNLVFPGRNDITKPMSEASINQVLKR
IGYHGKATGHGFRHTMSTILHEQGYNTAWIELQLAHVDKNTIRGTYNHAQYLEQRREMLQWYGEYVDGLAARGIAINIRK
KA

Sequences:

>Translated_402_residues
MALTDVKVRSAKPTDKPYKLTDGEGMHLMVHPNGSKYWRLQYRFDGKQKMLALGIYPEISLSEARQRRDEAKRQVANAID
PSEQKKVEKQARKATVENTFKAIALEWHEYKRPNWSKGYAEDIMEAFENDIFPDIGKRPIAEIKPLEILSSLRKLEKRGV
LDKLRKIRQACNQVFRYAIVTGRAESNPASELASALTPPKSVHYPHLLADELPAFLEALAAYSGSPITRLATKILMLTGV
RTIELRMAEWKEFDFDQRVWEVPVQRMKMRRPHLVPLSDQVVTALREIQAVTGRYNLVFPGRNDITKPMSEASINQVLKR
IGYHGKATGHGFRHTMSTILHEQGYNTAWIELQLAHVDKNTIRGTYNHAQYLEQRREMLQWYGEYVDGLAARGIAINIRK
KA
>Mature_401_residues
ALTDVKVRSAKPTDKPYKLTDGEGMHLMVHPNGSKYWRLQYRFDGKQKMLALGIYPEISLSEARQRRDEAKRQVANAIDP
SEQKKVEKQARKATVENTFKAIALEWHEYKRPNWSKGYAEDIMEAFENDIFPDIGKRPIAEIKPLEILSSLRKLEKRGVL
DKLRKIRQACNQVFRYAIVTGRAESNPASELASALTPPKSVHYPHLLADELPAFLEALAAYSGSPITRLATKILMLTGVR
TIELRMAEWKEFDFDQRVWEVPVQRMKMRRPHLVPLSDQVVTALREIQAVTGRYNLVFPGRNDITKPMSEASINQVLKRI
GYHGKATGHGFRHTMSTILHEQGYNTAWIELQLAHVDKNTIRGTYNHAQYLEQRREMLQWYGEYVDGLAARGIAINIRKK
A

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=386, Percent_Identity=44.559585492228, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI145693166, Length=392, Percent_Identity=41.5816326530612, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1788974, Length=397, Percent_Identity=37.0277078085642, Blast_Score=278, Evalue=5e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 46161; Mature: 46030

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTDVKVRSAKPTDKPYKLTDGEGMHLMVHPNGSKYWRLQYRFDGKQKMLALGIYPEIS
CCCCCCEECCCCCCCCCEEECCCCCEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCCC
LSEARQRRDEAKRQVANAIDPSEQKKVEKQARKATVENTFKAIALEWHEYKRPNWSKGYA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
EDIMEAFENDIFPDIGKRPIAEIKPLEILSSLRKLEKRGVLDKLRKIRQACNQVFRYAIV
HHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TGRAESNPASELASALTPPKSVHYPHLLADELPAFLEALAAYSGSPITRLATKILMLTGV
CCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
RTIELRMAEWKEFDFDQRVWEVPVQRMKMRRPHLVPLSDQVVTALREIQAVTGRYNLVFP
HHEEEEHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEC
GRNDITKPMSEASINQVLKRIGYHGKATGHGFRHTMSTILHEQGYNTAWIELQLAHVDKN
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCH
TIRGTYNHAQYLEQRREMLQWYGEYVDGLAARGIAINIRKKA
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCC
>Mature Secondary Structure 
ALTDVKVRSAKPTDKPYKLTDGEGMHLMVHPNGSKYWRLQYRFDGKQKMLALGIYPEIS
CCCCCEECCCCCCCCCEEECCCCCEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCCC
LSEARQRRDEAKRQVANAIDPSEQKKVEKQARKATVENTFKAIALEWHEYKRPNWSKGYA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
EDIMEAFENDIFPDIGKRPIAEIKPLEILSSLRKLEKRGVLDKLRKIRQACNQVFRYAIV
HHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TGRAESNPASELASALTPPKSVHYPHLLADELPAFLEALAAYSGSPITRLATKILMLTGV
CCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
RTIELRMAEWKEFDFDQRVWEVPVQRMKMRRPHLVPLSDQVVTALREIQAVTGRYNLVFP
HHEEEEHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEC
GRNDITKPMSEASINQVLKRIGYHGKATGHGFRHTMSTILHEQGYNTAWIELQLAHVDKN
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCH
TIRGTYNHAQYLEQRREMLQWYGEYVDGLAARGIAINIRKKA
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]