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The map label for this gene is lipB [H]

Identifier: 170025247

GI number: 170025247

Start: 3328646

End: 3329347

Strand: Direct

Name: lipB [H]

Synonym: YPK_3026

Alternate gene names: 170025247

Gene position: 3328646-3329347 (Clockwise)

Preceding gene: 170025246

Following gene: 170025248

Centisome position: 70.98

GC content: 48.72

Gene sequence:

>702_bases
ATGATGCCTCGCTTGCAACAACACAAGATCATTTTACGTCAGCTAGGGTTACAACCCTATGCACCCGTATCCCAAGCCAT
GCATAACTTTACTGAGTTTCGCACTGATACCACACCAGACGAAATCTGGTTGGTTGAGCATCAGCACGTCTTTACTCAGG
GTCAGGCTGGAAAAACTGAACATGTCCTGATGCCAGGTGATATTCCGGTTATTCAAAGTGATAGGGGCGGTCAGGTCACC
TACCATGGCCCCGGACAGCAAGTGATGTATGTCATGGTCGATCTCAAACGTGCCAAAATCGGTGTACGCCAGCTAGTCAC
CGCCATTGAGAATACGGTGATAGAGACTCTCGCCCATTTCAATATTGATTCACATGCTCGCCCAGATGCCCCCGGTGTCT
ATGTTGAACAACAGAAAATATGCTCACTGGGGCTACGCATTCGCCGAGGCTGTTCGTTTCACGGTTTAGCATTGAACATT
GCGATGGATCTGGAACCGTTCCAGCGCATTAATCCGTGCGGCTACGCGGGTATGCAGATGACACAAGTCAGTGCATTACA
ACCAGGGGTTACCGTTGCGGATGTGCAGCCTGTATTGGTTCGCGAATTTACCCGCCAATTAGGTTATCCGACGGCAAAAT
TGCAGCCGTGGTCATTATCAGACTATTTATTATCAAGCCATTCATCATCAAGCGTGTTGTAA

Upstream 100 bases:

>100_bases
AAATTATCCCCTTTAGCCATCAAATTATAGCGTTGAACTGTCAAAATAGTGGCCCCACTCAGCGGGGCTGGTATACTGGC
GCTACCATTTCTGTAACCAG

Downstream 100 bases:

>100_bases
ATGACTCATATGCCCGATGTAACTGAAACCGTGAACGCCACCATCGCGCCAAAAGTGAATGGCTGAACGAAACAGAGTAA
AGATGATATAATTTTTAAAC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MMPRLQQHKIILRQLGLQPYAPVSQAMHNFTEFRTDTTPDEIWLVEHQHVFTQGQAGKTEHVLMPGDIPVIQSDRGGQVT
YHGPGQQVMYVMVDLKRAKIGVRQLVTAIENTVIETLAHFNIDSHARPDAPGVYVEQQKICSLGLRIRRGCSFHGLALNI
AMDLEPFQRINPCGYAGMQMTQVSALQPGVTVADVQPVLVREFTRQLGYPTAKLQPWSLSDYLLSSHSSSSVL

Sequences:

>Translated_233_residues
MMPRLQQHKIILRQLGLQPYAPVSQAMHNFTEFRTDTTPDEIWLVEHQHVFTQGQAGKTEHVLMPGDIPVIQSDRGGQVT
YHGPGQQVMYVMVDLKRAKIGVRQLVTAIENTVIETLAHFNIDSHARPDAPGVYVEQQKICSLGLRIRRGCSFHGLALNI
AMDLEPFQRINPCGYAGMQMTQVSALQPGVTVADVQPVLVREFTRQLGYPTAKLQPWSLSDYLLSSHSSSSVL
>Mature_233_residues
MMPRLQQHKIILRQLGLQPYAPVSQAMHNFTEFRTDTTPDEIWLVEHQHVFTQGQAGKTEHVLMPGDIPVIQSDRGGQVT
YHGPGQQVMYVMVDLKRAKIGVRQLVTAIENTVIETLAHFNIDSHARPDAPGVYVEQQKICSLGLRIRRGCSFHGLALNI
AMDLEPFQRINPCGYAGMQMTQVSALQPGVTVADVQPVLVREFTRQLGYPTAKLQPWSLSDYLLSSHSSSSVL

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=134, Percent_Identity=38.0597014925373, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI87081767, Length=206, Percent_Identity=67.9611650485437, Blast_Score=304, Evalue=3e-84,
Organism=Saccharomyces cerevisiae, GI6323268, Length=157, Percent_Identity=35.6687898089172, Blast_Score=91, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355483, Length=194, Percent_Identity=30.9278350515464, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 26025; Mature: 26025

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPRLQQHKIILRQLGLQPYAPVSQAMHNFTEFRTDTTPDEIWLVEHQHVFTQGQAGKTE
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCEEEECCCCCCCC
HVLMPGDIPVIQSDRGGQVTYHGPGQQVMYVMVDLKRAKIGVRQLVTAIENTVIETLAHF
EEEECCCCCEEECCCCCEEEEECCCCEEEEHEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
NIDSHARPDAPGVYVEQQKICSLGLRIRRGCSFHGLALNIAMDLEPFQRINPCGYAGMQM
CCCCCCCCCCCCEEECHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHCCCCCCCCCHH
TQVSALQPGVTVADVQPVLVREFTRQLGYPTAKLQPWSLSDYLLSSHSSSSVL
HHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MMPRLQQHKIILRQLGLQPYAPVSQAMHNFTEFRTDTTPDEIWLVEHQHVFTQGQAGKTE
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCEEEECCCCCCCC
HVLMPGDIPVIQSDRGGQVTYHGPGQQVMYVMVDLKRAKIGVRQLVTAIENTVIETLAHF
EEEECCCCCEEECCCCCEEEEECCCCEEEEHEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
NIDSHARPDAPGVYVEQQKICSLGLRIRRGCSFHGLALNIAMDLEPFQRINPCGYAGMQM
CCCCCCCCCCCCEEECHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHCCCCCCCCCHH
TQVSALQPGVTVADVQPVLVREFTRQLGYPTAKLQPWSLSDYLLSSHSSSSVL
HHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA