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The map label for this gene is ybfF [H]

Identifier: 170025208

GI number: 170025208

Start: 3282651

End: 3283418

Strand: Direct

Name: ybfF [H]

Synonym: YPK_2986

Alternate gene names: 170025208

Gene position: 3282651-3283418 (Clockwise)

Preceding gene: 170025207

Following gene: 170025209

Centisome position: 70.0

GC content: 48.7

Gene sequence:

>768_bases
ATGAAATTAAATTTCCGCTTACAAAACGCACCGTCCCCTACCTCAGCCCTGCCAATCATTCTGATCCATGGATTATTCGG
CAATTTGGATAATCTAGGTGTATTGGCTCGCGATCTGCAGCAACACCATAATGTTATTCAGGTCGATTTACGTAACCATG
GCTTATCTCCCCGTGCACCACAGATGGATTATCCCGTAATGGCGCAGGATGTGTTGGCGTTAATGGATGAACTGGCAATA
ACACAAGCCATTATTATCGGCCATTCCATGGGCGGAAAAGTGGCGATGGCAATGACCGCGCTTGCCCCAAATCGGGTTGA
AAAACTGGTAGCGATCGATATTGCGCCAGTGAATTATCAGGTACGCCGTCATGACACTATTTTCGCCGCGCTTAATGCTG
TGAGTGCCGCTGGTGTGACTCAGCGCAACGAAGCCGCTCAACTGATGCGCACGTTAATAAAAGAAGAAGGGGTTATTCAG
TTCTTACTAAAATCATTTCAGGGCGGTGAATGGCGATTTAATGTCCCTGCCCTCTGGGATCAGTACGAGAACATTATTGG
CTGGCAGCCCATTCCCCCTTGGCCACATCCTATTTTGTTTATCCGTGGTGAGTTATCTCCTTATATTCAAGACAGCTATC
GAGATGATATTGCCCGTCAGTTCCCACAAGCCCGTGCGCATGTCGTCGCCGGAACCGGTCATTGGGTCCATGCGGAAAAA
CCAGATTCTGTGTTGCGGGCAATCCACCGTTTCCTTGATGCCGCATAA

Upstream 100 bases:

>100_bases
TCTGCGCCTAAGGTCGCCCTCACTTAAGCATCAGAAAGATCAACTTCAGGCATTCATGGTACCCTAACCAGAATATCTCT
CAGTATGAACGTCGCTCGCT

Downstream 100 bases:

>100_bases
CGCAAGATTCCCGTCATGTTGATTAAAAATCATACCTTGGGGAAAAGAAATGTATTTCCTGTGGAAAAATTGTGCAAAAA
AGAACGAATTAGCCCAATCT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKLNFRLQNAPSPTSALPIILIHGLFGNLDNLGVLARDLQQHHNVIQVDLRNHGLSPRAPQMDYPVMAQDVLALMDELAI
TQAIIIGHSMGGKVAMAMTALAPNRVEKLVAIDIAPVNYQVRRHDTIFAALNAVSAAGVTQRNEAAQLMRTLIKEEGVIQ
FLLKSFQGGEWRFNVPALWDQYENIIGWQPIPPWPHPILFIRGELSPYIQDSYRDDIARQFPQARAHVVAGTGHWVHAEK
PDSVLRAIHRFLDAA

Sequences:

>Translated_255_residues
MKLNFRLQNAPSPTSALPIILIHGLFGNLDNLGVLARDLQQHHNVIQVDLRNHGLSPRAPQMDYPVMAQDVLALMDELAI
TQAIIIGHSMGGKVAMAMTALAPNRVEKLVAIDIAPVNYQVRRHDTIFAALNAVSAAGVTQRNEAAQLMRTLIKEEGVIQ
FLLKSFQGGEWRFNVPALWDQYENIIGWQPIPPWPHPILFIRGELSPYIQDSYRDDIARQFPQARAHVVAGTGHWVHAEK
PDSVLRAIHRFLDAA
>Mature_255_residues
MKLNFRLQNAPSPTSALPIILIHGLFGNLDNLGVLARDLQQHHNVIQVDLRNHGLSPRAPQMDYPVMAQDVLALMDELAI
TQAIIIGHSMGGKVAMAMTALAPNRVEKLVAIDIAPVNYQVRRHDTIFAALNAVSAAGVTQRNEAAQLMRTLIKEEGVIQ
FLLKSFQGGEWRFNVPALWDQYENIIGWQPIPPWPHPILFIRGELSPYIQDSYRDDIARQFPQARAHVVAGTGHWVHAEK
PDSVLRAIHRFLDAA

Specific function: Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200008, Length=265, Percent_Identity=35.0943396226415, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI23200012, Length=251, Percent_Identity=35.8565737051793, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI223941839, Length=256, Percent_Identity=30.859375, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1786902, Length=253, Percent_Identity=66.0079051383399, Blast_Score=353, Evalue=8e-99,
Organism=Caenorhabditis elegans, GI17508535, Length=267, Percent_Identity=30.3370786516854, Blast_Score=140, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17507063, Length=260, Percent_Identity=28.8461538461538, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6321468, Length=265, Percent_Identity=33.5849056603774, Blast_Score=108, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24640348, Length=272, Percent_Identity=27.2058823529412, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24646055, Length=271, Percent_Identity=25.4612546125461, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 28470; Mature: 28470

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNFRLQNAPSPTSALPIILIHGLFGNLDNLGVLARDLQQHHNVIQVDLRNHGLSPRAP
CEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCC
QMDYPVMAQDVLALMDELAITQAIIIGHSMGGKVAMAMTALAPNRVEKLVAIDIAPVNYQ
CCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHCHHHHHHEEEEEEECCCEE
VRRHDTIFAALNAVSAAGVTQRNEAAQLMRTLIKEEGVIQFLLKSFQGGEWRFNVPALWD
EHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCHHHH
QYENIIGWQPIPPWPHPILFIRGELSPYIQDSYRDDIARQFPQARAHVVAGTGHWVHAEK
HHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHEEEEECCCCEEECCC
PDSVLRAIHRFLDAA
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLNFRLQNAPSPTSALPIILIHGLFGNLDNLGVLARDLQQHHNVIQVDLRNHGLSPRAP
CEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCC
QMDYPVMAQDVLALMDELAITQAIIIGHSMGGKVAMAMTALAPNRVEKLVAIDIAPVNYQ
CCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHCHHHHHHEEEEEEECCCEE
VRRHDTIFAALNAVSAAGVTQRNEAAQLMRTLIKEEGVIQFLLKSFQGGEWRFNVPALWD
EHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCHHHH
QYENIIGWQPIPPWPHPILFIRGELSPYIQDSYRDDIARQFPQARAHVVAGTGHWVHAEK
HHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHEEEEECCCCEEECCC
PDSVLRAIHRFLDAA
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]