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The map label for this gene is pla [H]

Identifier: 170025055

GI number: 170025055

Start: 3115194

End: 3116129

Strand: Direct

Name: pla [H]

Synonym: YPK_2831

Alternate gene names: 170025055

Gene position: 3115194-3116129 (Clockwise)

Preceding gene: 170025054

Following gene: 170025057

Centisome position: 66.43

GC content: 40.17

Gene sequence:

>936_bases
ATGAATAAAGAAGCACTAGCAAAAAAAGGGGCCGGCGCACTGGCATTACTTATTCTGAGTCATCCTGCCGTGGCCAACAA
TAATACGGATACAGCAGGCAACGTGACTGTCAGCACCTCGCTTGGATTATTGAATGCCGAGTCAATCGAATCTGTTTATC
AATCCAGTGAACCTAACCAAAAGCTCAGTCAGTTAAACTGGGAAGCCAACAATACACCCATCGTAAAAATGGATATCGCC
TGGGATGCGTTACCCCGACTAACCCTAACAGCCAAGGGCTGGAGCACGCTTTCATCAGGTCAGGGAGTGATGGATAATTA
TGATTGGCTTACCCCACAACAAACACAGTGGAGTGATTGGTCTCATCACGAAAAAACCAATTTGAATTATGCCAATGAAA
TAGACATAAATGCGAATATTTGGTTTTTAAAACAATACAATTATCGGGTTGGGCCTATTTTAGGTTATCAGGAAAACAAT
CAAAGCTGGACAGCTTGTGGTGGTAACTTTAATTATAATAATGGTGCAAATATCTTTACTGCTCCTGACGGCATTGTTGG
CGTTGGTTATAAACAAAAATTTGATATGCTTTATATTGGCTTAGCAGGAAGTTATCGCTATCAACAATTAGAATTTAATG
CCTTATTGAAATACAGCAATTGGGTTAATGCCAATGCATACGATAATCACTATAACCGCAAAGTTTCATTTAAAGACAGT
AGCAAGAATTCAAGTTACTACTCGGCCGTTATTGATGTTGGATATTACTTCACAGAAAATACTAAATTTTTCGTGGAAGC
GGCTTGGAACCAATATTCTGAAGGGCGAGGAGAGACACAAACGCTCAACTACGCCACCGGGAATGTTGGCAGTAGTAAAT
CAGGTATTGAGTATCAAAGCCAAACCCTTACCGTGGGTTTTCAGTACAAATTTTAA

Upstream 100 bases:

>100_bases
ATTAATTTTTTTAAAATTTTTTTAATCTTTAATTTAAAAAATTAAATCTTTATCGATGTTAGTTTTAATCATGATGATTT
AAGTGAATTAAAGGTTAAAA

Downstream 100 bases:

>100_bases
TCGCATAAAAACACAATAAGCGCCGGAAATCGAAAATCAGGGGTTGTGTACAATTGATCTGTACACAATCGATCTGTACA
CAATACATCTGTACACAATA

Product: outer membrane protease

Products: NA

Alternate protein names: Plasminogen activator [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MNKEALAKKGAGALALLILSHPAVANNNTDTAGNVTVSTSLGLLNAESIESVYQSSEPNQKLSQLNWEANNTPIVKMDIA
WDALPRLTLTAKGWSTLSSGQGVMDNYDWLTPQQTQWSDWSHHEKTNLNYANEIDINANIWFLKQYNYRVGPILGYQENN
QSWTACGGNFNYNNGANIFTAPDGIVGVGYKQKFDMLYIGLAGSYRYQQLEFNALLKYSNWVNANAYDNHYNRKVSFKDS
SKNSSYYSAVIDVGYYFTENTKFFVEAAWNQYSEGRGETQTLNYATGNVGSSKSGIEYQSQTLTVGFQYKF

Sequences:

>Translated_311_residues
MNKEALAKKGAGALALLILSHPAVANNNTDTAGNVTVSTSLGLLNAESIESVYQSSEPNQKLSQLNWEANNTPIVKMDIA
WDALPRLTLTAKGWSTLSSGQGVMDNYDWLTPQQTQWSDWSHHEKTNLNYANEIDINANIWFLKQYNYRVGPILGYQENN
QSWTACGGNFNYNNGANIFTAPDGIVGVGYKQKFDMLYIGLAGSYRYQQLEFNALLKYSNWVNANAYDNHYNRKVSFKDS
SKNSSYYSAVIDVGYYFTENTKFFVEAAWNQYSEGRGETQTLNYATGNVGSSKSGIEYQSQTLTVGFQYKF
>Mature_311_residues
MNKEALAKKGAGALALLILSHPAVANNNTDTAGNVTVSTSLGLLNAESIESVYQSSEPNQKLSQLNWEANNTPIVKMDIA
WDALPRLTLTAKGWSTLSSGQGVMDNYDWLTPQQTQWSDWSHHEKTNLNYANEIDINANIWFLKQYNYRVGPILGYQENN
QSWTACGGNFNYNNGANIFTAPDGIVGVGYKQKFDMLYIGLAGSYRYQQLEFNALLKYSNWVNANAYDNHYNRKVSFKDS
SKNSSYYSAVIDVGYYFTENTKFFVEAAWNQYSEGRGETQTLNYATGNVGSSKSGIEYQSQTLTVGFQYKF

Specific function: Seems to play an essential role in plague transmission by mediating flea blockage in a temperature-dependent fashion. Fibrinolytic activity prevails at 37 degrees Celsius whereas coagulase expression predominates at lower temperatures (<30 degrees Celsius

COG id: COG4571

COG function: function code M; Outer membrane protease

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A26 family [H]

Homologues:

Organism=Escherichia coli, GI1786777, Length=290, Percent_Identity=39.3103448275862, Blast_Score=218, Evalue=4e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020080
- InterPro:   IPR000036
- InterPro:   IPR020079 [H]

Pfam domain/function: PF01278 Omptin [H]

EC number: =3.4.23.48 [H]

Molecular weight: Translated: 34871; Mature: 34871

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEALAKKGAGALALLILSHPAVANNNTDTAGNVTVSTSLGLLNAESIESVYQSSEPNQ
CCHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCC
KLSQLNWEANNTPIVKMDIAWDALPRLTLTAKGWSTLSSGQGVMDNYDWLTPQQTQWSDW
HHHHCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SHHEKTNLNYANEIDINANIWFLKQYNYRVGPILGYQENNQSWTACGGNFNYNNGANIFT
CCCCCCCCCCCEEEECCCEEEEEEECCCEECCEEEEECCCCEEEEECCCCCCCCCCEEEE
APDGIVGVGYKQKFDMLYIGLAGSYRYQQLEFNALLKYSNWVNANAYDNHYNRKVSFKDS
CCCCEEECCCCCCCEEEEEEECCCCEEEEEEEEEEEEECCCCCCCCCCCCCCEEEEECCC
SKNSSYYSAVIDVGYYFTENTKFFVEAAWNQYSEGRGETQTLNYATGNVGSSKSGIEYQS
CCCCCEEEEEEEEEEEEECCCEEEEEEECCHHCCCCCCCEEEEEEECCCCCCCCCCEEEC
QTLTVGFQYKF
EEEEEEEEECC
>Mature Secondary Structure
MNKEALAKKGAGALALLILSHPAVANNNTDTAGNVTVSTSLGLLNAESIESVYQSSEPNQ
CCHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCC
KLSQLNWEANNTPIVKMDIAWDALPRLTLTAKGWSTLSSGQGVMDNYDWLTPQQTQWSDW
HHHHCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SHHEKTNLNYANEIDINANIWFLKQYNYRVGPILGYQENNQSWTACGGNFNYNNGANIFT
CCCCCCCCCCCEEEECCCEEEEEEECCCEECCEEEEECCCCEEEEECCCCCCCCCCEEEE
APDGIVGVGYKQKFDMLYIGLAGSYRYQQLEFNALLKYSNWVNANAYDNHYNRKVSFKDS
CCCCEEECCCCCCCEEEEEEECCCCEEEEEEEEEEEEECCCCCCCCCCCCCCEEEEECCC
SKNSSYYSAVIDVGYYFTENTKFFVEAAWNQYSEGRGETQTLNYATGNVGSSKSGIEYQS
CCCCCEEEEEEEEEEEEECCCEEEEEEECCHHCCCCCCCEEEEEEECCCCCCCCCCEEEC
QTLTVGFQYKF
EEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2526282; 2651310; 9748454; 11586360 [H]