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The map label for this gene is pcbC [H]

Identifier: 170024969

GI number: 170024969

Start: 3026584

End: 3027651

Strand: Reverse

Name: pcbC [H]

Synonym: YPK_2744

Alternate gene names: 170024969

Gene position: 3027651-3026584 (Counterclockwise)

Preceding gene: 170024970

Following gene: 170024968

Centisome position: 64.56

GC content: 53.93

Gene sequence:

>1068_bases
ATGGGCCAACAGGTATTAAGTCATCAATCAGTCAGTAGCCAATGGCTGGCGAATCAGTCAGCGACCAGCACATTACCGCT
ACTGGATCTTTCACTACTCAGTGGCAGTGATACCGAACGCCAGGCATTTTTAACGGCCTTGCGCCATGCGGCGCGGGATG
TCGGTTTTTTTTATCTGACCGGGCACGGCATTGATACCTCGTTACTACGGCAGATACAAATCCTATCACGGCAGTTCTTT
GCGCTGAGCGATGAAGAAAAACTGTCAATCGCCATGATTCGCTCCCCGCATTTTCGCGGCTATAACCGTGCTGCTTCGGA
ATTCACCCGTGGTCAGCCAGATTGGCGCGAGCAATTCGACATTGGTGCTGAAAGAACCCCATTACCACAAATCGCAGGCG
CACCCAGTTGGACACGGCTGCAAGGCCCGAACCAATGGCCAACCGCACTGCCAGAATTAAAACCTGTGTTATTGCAGTGG
CAAGAGGAGATGACCCGCATGTCACTGCGCCTCCTGCGGGCATTCGCACTGGCATTGGATCTGGATGAGCAGGCTTTTGA
TGCGTTGTACGGTGATAAACCCAATGAACATATCAAGCTGATTCGTTATCCAGGGCGGGAGGCGACCCAAAGTGGGCAGG
GGGTTGGTGCGCACAAAGATTCGGGTTTCCTTAGCTTCTTACTGCAAGACACGCAACGCGGTTTACAAGTCGAAGTGGAA
GAGGGCCAATGGATTGATGCCGTACCGCGTGAAGGCACTTTTGTGGTGAATATCGGTGAGTTGTTGGAACTGGCCAGTAA
TGGTTACTTGCGTGCCACCGTACACCGGGTGGAAACCCCACCCGCCGGGATTGACCGCCTGTCTATTGCCTTCTTTTTAG
GTGCGCGTTTGGATGCGGTGGTGCCACTGTATCCATTATCTGCCGCATTGGCGGCGGAAGCTCGCGGGCCAGCCAGTGAT
CCGCACAACCCGTTGTTCCGTGATGTTGGGCTGAATTATCTCAAGGGGCGTTTGCGGTCACATCCCGATGTGGCGGAGCG
GTATTACTCCGATGCCCTTTGTCCTTGA

Upstream 100 bases:

>100_bases
GCTCAGGAGCTGATTATGCTGATTATTCGTTTGTATGGTTGTCCCGTTTTCATTGGTTTCGAACCCCAAAAACCACCAAT
TGAAACGACTGGAGTGAAAA

Downstream 100 bases:

>100_bases
AGTGGTAGGGGTGTTAGCTGCGCGCACTTACCCGAATCACTGACGTGAGTCAGCTCATCGGGATTCGTTTGCTGCATGCA
CTTACTCGAATCACTGATGT

Product: 2OG-Fe(II) oxygenase

Products: NA

Alternate protein names: IPNS [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MGQQVLSHQSVSSQWLANQSATSTLPLLDLSLLSGSDTERQAFLTALRHAARDVGFFYLTGHGIDTSLLRQIQILSRQFF
ALSDEEKLSIAMIRSPHFRGYNRAASEFTRGQPDWREQFDIGAERTPLPQIAGAPSWTRLQGPNQWPTALPELKPVLLQW
QEEMTRMSLRLLRAFALALDLDEQAFDALYGDKPNEHIKLIRYPGREATQSGQGVGAHKDSGFLSFLLQDTQRGLQVEVE
EGQWIDAVPREGTFVVNIGELLELASNGYLRATVHRVETPPAGIDRLSIAFFLGARLDAVVPLYPLSAALAAEARGPASD
PHNPLFRDVGLNYLKGRLRSHPDVAERYYSDALCP

Sequences:

>Translated_355_residues
MGQQVLSHQSVSSQWLANQSATSTLPLLDLSLLSGSDTERQAFLTALRHAARDVGFFYLTGHGIDTSLLRQIQILSRQFF
ALSDEEKLSIAMIRSPHFRGYNRAASEFTRGQPDWREQFDIGAERTPLPQIAGAPSWTRLQGPNQWPTALPELKPVLLQW
QEEMTRMSLRLLRAFALALDLDEQAFDALYGDKPNEHIKLIRYPGREATQSGQGVGAHKDSGFLSFLLQDTQRGLQVEVE
EGQWIDAVPREGTFVVNIGELLELASNGYLRATVHRVETPPAGIDRLSIAFFLGARLDAVVPLYPLSAALAAEARGPASD
PHNPLFRDVGLNYLKGRLRSHPDVAERYYSDALCP
>Mature_354_residues
GQQVLSHQSVSSQWLANQSATSTLPLLDLSLLSGSDTERQAFLTALRHAARDVGFFYLTGHGIDTSLLRQIQILSRQFFA
LSDEEKLSIAMIRSPHFRGYNRAASEFTRGQPDWREQFDIGAERTPLPQIAGAPSWTRLQGPNQWPTALPELKPVLLQWQ
EEMTRMSLRLLRAFALALDLDEQAFDALYGDKPNEHIKLIRYPGREATQSGQGVGAHKDSGFLSFLLQDTQRGLQVEVEE
GQWIDAVPREGTFVVNIGELLELASNGYLRATVHRVETPPAGIDRLSIAFFLGARLDAVVPLYPLSAALAAEARGPASDP
HNPLFRDVGLNYLKGRLRSHPDVAERYYSDALCP

Specific function: Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin [H]

COG id: COG3491

COG function: function code R; Isopenicillin N synthase and related dioxygenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Drosophila melanogaster, GI28572124, Length=289, Percent_Identity=24.2214532871972, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002057
- InterPro:   IPR002283
- InterPro:   IPR005123 [H]

Pfam domain/function: PF03171 2OG-FeII_Oxy [H]

EC number: =1.21.3.1 [H]

Molecular weight: Translated: 39499; Mature: 39368

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQQVLSHQSVSSQWLANQSATSTLPLLDLSLLSGSDTERQAFLTALRHAARDVGFFYLT
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEE
GHGIDTSLLRQIQILSRQFFALSDEEKLSIAMIRSPHFRGYNRAASEFTRGQPDWREQFD
CCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHC
IGAERTPLPQIAGAPSWTRLQGPNQWPTALPELKPVLLQWQEEMTRMSLRLLRAFALALD
CCCCCCCCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDEQAFDALYGDKPNEHIKLIRYPGREATQSGQGVGAHKDSGFLSFLLQDTQRGLQVEVE
CCHHHHHHHHCCCCCCCEEEEECCCCHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEC
EGQWIDAVPREGTFVVNIGELLELASNGYLRATVHRVETPPAGIDRLSIAFFLGARLDAV
CCCEEECCCCCCEEEEEHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
VPLYPLSAALAAEARGPASDPHNPLFRDVGLNYLKGRLRSHPDVAERYYSDALCP
CCCHHHHHHHHHHCCCCCCCCCCCHHHHHCHHHHHHHHCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
GQQVLSHQSVSSQWLANQSATSTLPLLDLSLLSGSDTERQAFLTALRHAARDVGFFYLT
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEE
GHGIDTSLLRQIQILSRQFFALSDEEKLSIAMIRSPHFRGYNRAASEFTRGQPDWREQFD
CCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHC
IGAERTPLPQIAGAPSWTRLQGPNQWPTALPELKPVLLQWQEEMTRMSLRLLRAFALALD
CCCCCCCCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDEQAFDALYGDKPNEHIKLIRYPGREATQSGQGVGAHKDSGFLSFLLQDTQRGLQVEVE
CCHHHHHHHHCCCCCCCEEEEECCCCHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEC
EGQWIDAVPREGTFVVNIGELLELASNGYLRATVHRVETPPAGIDRLSIAFFLGARLDAV
CCCEEECCCCCCEEEEEHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
VPLYPLSAALAAEARGPASDPHNPLFRDVGLNYLKGRLRSHPDVAERYYSDALCP
CCCHHHHHHHHHHCCCCCCCCCCCHHHHHCHHHHHHHHCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3130293 [H]