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The map label for this gene is pksE [H]

Identifier: 170024827

GI number: 170024827

Start: 2857435

End: 2858283

Strand: Reverse

Name: pksE [H]

Synonym: YPK_2602

Alternate gene names: 170024827

Gene position: 2858283-2857435 (Counterclockwise)

Preceding gene: 170024828

Following gene: 170024826

Centisome position: 60.95

GC content: 40.4

Gene sequence:

>849_bases
ATGACATCAAATCATGCCATTTTATTTCCAGGACAAGGTGCGCAGCGTGCAGATATGGGCGCTCCGATATTTGATATCTA
CACCAAATTAGTGGCAGAGGCTGAAGAGATCGCTGAATTTAGTATTCGTGATTTTTTTCGGGATTGTCCTGCGGATCGGT
TTAGCAACACCCTTTATGTTCAGCCAGCTTTATATACAGTTAATGCTTTGTATTATCGTCGATATATTGATGAGTCTGGG
GCGTTACCATTATTTTTAGCGGGTCATTCGCTTGGCGAATTTAATGCACTACATGCCGCCGGTGTATTCGACTTTGAAAC
AGGATTACGTCTGGTAAAAAAAAGAGCCTTGCTAATGTCTGAAAGCCAGGGGGGAGGTATGGCTGCTGTTCTTGGTCTGA
ATGCTGAAGAAGTGGTCAAAATCCTTGAGGAAAATAATCTGTTTGATCTCACTATAGCCAATATTAATTCCGACTTGCAA
ATGGTGATATCAGGGCCGATTGAGAGTATCAAACAGGCAGAAGAGATATTTTATGATAACTATGCGATTAAATATGTTGT
ACTGAGAGTGAGTGGTGCTTTTCATAGTACGATGTTAAATACCGCGGCCATAAAATTCAGTGAATATATCCAAGGTTTTT
CATTTTTACCGCCTCAAATACCGGTTATCGCCAATAGAACAGCCTGTCCATATACCCTATCTTCTGTAATAAAAAATCTC
TCTGAGCATATAAATCGACCTGTCATGTGGCGGCAATCGGTTATATATATGCGGACACACGGTGTAGAAAGATTTACTGA
GATGGGAGAGACTTCTTTACTGCTGGATATGGTTAAGAATATTTTGTAG

Upstream 100 bases:

>100_bases
AGACATTTCTGCACTATGGCCTACGGAGGGGCCATATTCAGAACTGAAATAGGAAAGCCTGCCTGTTTGTGTAACACAAT
CACAATAAAGAGGGTGATAC

Downstream 100 bases:

>100_bases
TAGTAGGAATTTGAGGTTCAATGGTACGGAAGTGTACAATAAGGAATATTTTCTTATGGATTATCTCTCCTTCCTGCTGC
CTTTGGTTTTGCCCCAATTA

Product: putative acyltransferase

Products: NA

Alternate protein names: Malonyl CoA-acyl carrier protein transacylase; MCT [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTSNHAILFPGQGAQRADMGAPIFDIYTKLVAEAEEIAEFSIRDFFRDCPADRFSNTLYVQPALYTVNALYYRRYIDESG
ALPLFLAGHSLGEFNALHAAGVFDFETGLRLVKKRALLMSESQGGGMAAVLGLNAEEVVKILEENNLFDLTIANINSDLQ
MVISGPIESIKQAEEIFYDNYAIKYVVLRVSGAFHSTMLNTAAIKFSEYIQGFSFLPPQIPVIANRTACPYTLSSVIKNL
SEHINRPVMWRQSVIYMRTHGVERFTEMGETSLLLDMVKNIL

Sequences:

>Translated_282_residues
MTSNHAILFPGQGAQRADMGAPIFDIYTKLVAEAEEIAEFSIRDFFRDCPADRFSNTLYVQPALYTVNALYYRRYIDESG
ALPLFLAGHSLGEFNALHAAGVFDFETGLRLVKKRALLMSESQGGGMAAVLGLNAEEVVKILEENNLFDLTIANINSDLQ
MVISGPIESIKQAEEIFYDNYAIKYVVLRVSGAFHSTMLNTAAIKFSEYIQGFSFLPPQIPVIANRTACPYTLSSVIKNL
SEHINRPVMWRQSVIYMRTHGVERFTEMGETSLLLDMVKNIL
>Mature_281_residues
TSNHAILFPGQGAQRADMGAPIFDIYTKLVAEAEEIAEFSIRDFFRDCPADRFSNTLYVQPALYTVNALYYRRYIDESGA
LPLFLAGHSLGEFNALHAAGVFDFETGLRLVKKRALLMSESQGGGMAAVLGLNAEEVVKILEENNLFDLTIANINSDLQM
VISGPIESIKQAEEIFYDNYAIKYVVLRVSGAFHSTMLNTAAIKFSEYIQGFSFLPPQIPVIANRTACPYTLSSVIKNLS
EHINRPVMWRQSVIYMRTHGVERFTEMGETSLLLDMVKNIL

Specific function: Probably involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably has an acyl transferase activity and could also have a flavin mononucleotide-dependent oxidoreductas

COG id: COG0331

COG function: function code I; (acyl-carrier-protein) S-malonyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the fabD family [H]

Homologues:

Organism=Homo sapiens, GI51243059, Length=281, Percent_Identity=29.5373665480427, Blast_Score=78, Evalue=7e-15,
Organism=Escherichia coli, GI1787334, Length=289, Percent_Identity=33.2179930795848, Blast_Score=144, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI71984332, Length=288, Percent_Identity=29.1666666666667, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17550940, Length=196, Percent_Identity=25.5102040816327, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24665753, Length=302, Percent_Identity=25.8278145695364, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR001227
- InterPro:   IPR014043
- InterPro:   IPR016035
- InterPro:   IPR013785
- InterPro:   IPR004410
- InterPro:   IPR016036
- InterPro:   IPR014179 [H]

Pfam domain/function: PF00698 Acyl_transf_1; PF03060 NPD [H]

EC number: =2.3.1.39 [H]

Molecular weight: Translated: 31541; Mature: 31409

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSNHAILFPGQGAQRADMGAPIFDIYTKLVAEAEEIAEFSIRDFFRDCPADRFSNTLYV
CCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEE
QPALYTVNALYYRRYIDESGALPLFLAGHSLGEFNALHAAGVFDFETGLRLVKKRALLMS
CHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHC
ESQGGGMAAVLGLNAEEVVKILEENNLFDLTIANINSDLQMVISGPIESIKQAEEIFYDN
CCCCCCEEEEECCCHHHHHHHHHCCCEEEEEEECCCCCHHEEEHHHHHHHHHHHHHHHCC
YAIKYVVLRVSGAFHSTMLNTAAIKFSEYIQGFSFLPPQIPVIANRTACPYTLSSVIKNL
CEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHH
SEHINRPVMWRQSVIYMRTHGVERFTEMGETSLLLDMVKNIL
HHHCCCCEEHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TSNHAILFPGQGAQRADMGAPIFDIYTKLVAEAEEIAEFSIRDFFRDCPADRFSNTLYV
CCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEE
QPALYTVNALYYRRYIDESGALPLFLAGHSLGEFNALHAAGVFDFETGLRLVKKRALLMS
CHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHC
ESQGGGMAAVLGLNAEEVVKILEENNLFDLTIANINSDLQMVISGPIESIKQAEEIFYDN
CCCCCCEEEEECCCHHHHHHHHHCCCEEEEEEECCCCCHHEEEHHHHHHHHHHHHHHHCC
YAIKYVVLRVSGAFHSTMLNTAAIKFSEYIQGFSFLPPQIPVIANRTACPYTLSSVIKNL
CEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHH
SEHINRPVMWRQSVIYMRTHGVERFTEMGETSLLLDMVKNIL
HHHCCCCEEHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]