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The map label for this gene is ychM [C]

Identifier: 170024286

GI number: 170024286

Start: 2282874

End: 2284613

Strand: Reverse

Name: ychM [C]

Synonym: YPK_2051

Alternate gene names: 170024286

Gene position: 2284613-2282874 (Counterclockwise)

Preceding gene: 170024287

Following gene: 170024284

Centisome position: 48.72

GC content: 48.1

Gene sequence:

>1740_bases
ATGCAGAAAAAATCCCTTCGGTATTGGATGCCGGGTTTGACCCAATTAATGGCCTATGAGCGTGATTGGATAAAGCCTGA
CCTCCGAGCGGGGTTATCGGTAGCCGCAGTCGCATTACCTATTGCCATTGCTTATGCCGAGTTGACGGGCGTTAGCGCCG
CAGTCGGGTTGTACTCCTGTATTTTGCCGATGATAGCCTATGCGTTTTTTGGTTCATCGCGGCAATTAATTGTTGGGCCA
GATGCCGCAACCTGTGCAGTGATTGCGGCTGTGGTAGCCCCGTTGGCTGCAGGGAATAGTGAGGTTCATTGGCAATTAAC
CATCATGATGACGTTGATGATGGGGTCATGGTGTCTGGTCGCCAGCCGCTTTAAGCTGGGAGCGTTGGCGGATTTATTAT
CGCGGCCCATACTAACCGGTTTACTGAATGGTGTGGCGATCACGATTATTGTCGATCAATTAGGTAAGGTTTTTGGCTTT
ATGACTCGTCCGCCACAGCTCATTGAACGAGTCTTGGCGCTCCCCTATAACATGTTTAACAGCCATTTACCTACGGTTGC
CATCTCCTTACTGACCTTTGTGGTGCTATATGGGGTCAAATGGTTGAGACCCAATTGGCCCGCACCCTTGCTGGCGATTG
TCATCGCAACCTTTGTGTCTTGGGCCGCTAATATGCAGCAATTTGGTGTTGATGTGGTTGGCGGTTTTGAGGGGGGGTTA
CCCATAGTACATTGGCCTGATTTTCAACCCGGCCTGTTACGCGATATGGTCATCCCAGCCCTTAACCTCGCTGTGGTCAG
TTTTGTCAGTATGATGCTCACCGCTCGCAGTTTTGCAGCGAAAAATGGTTATGACGTTGATGCTGATGTTGAACTTCGTG
CATTGGGTATTACGAATATTGTTTCAGCTTTATCACAAGGTTTTGCTATTAGTGGTGCCAGTACACGCACGGCGGTGAAT
GATGCTAACAATGGTAAAAGCCAGTTGGTTTCTATCATTGCGGCTCTGGTGATCGCGATGGTCCTGTTGTTCTTAACCCG
CCCACTGCAATTTATTCCCATTGCGGCATTAGGTGTCGTGTTGATTTACGCTGCCTGGTCGTTACTGAGCTTTAAAAGTT
TGTGGCAACTGCGTAAACGCAATACTCAAGCTTTCTATCTGGCTATTTTTACATTTGTTAGTGTGGTTTTGGTTGGTGTT
ATCAGCGGGATCGGCCTGGCGGTATTGTTAGGGCTGTTGCAATTTTTGCGGACCGTATTCCGGCCGACAGAGCAACTGTT
GGGTGTTAACGCTGATGGCATGATCCATTCCATGGGTAACGGCAATGGTATTAAAGCCGTTCCTGGGGTAATGATTTACC
GTTTTAACTCACCACTGACCTATTTTAATGTCGCTTATTTTAAGCGGCGCATCCTGAATTTGGTGGATAGCACGCCACAT
CCTGCTGACTGGGTCGTTATTGATGCTGTTGCCAGTTTCACCTATGCTGATATCAGTGTATTGGCCGCCATTGATGAACT
TAAACGTGACCTTAAGCAGCGGAATATTAAACTGATATTGGCGGGGCGACGGACAGAATTAACCCGCTGGTTCCGTATTA
ATCGCCTGAAAAGCCATGATGACGATTTGGTCTTGGTCCCAGATTTATATCTGGCGCTTAAGTTGATTCAGAGTAAACGG
CGGGTAGCAGAGAAACAGGAACACGAGTCAGAAACGGGATTGGAACCTGAAATAGGCTGA

Upstream 100 bases:

>100_bases
TACGAAAAATCAGTAAGGAACACGGTTTGCATATTGCCAGTGATGAGCTAACCCAGCATGATGAAAGATAAATGTTGGGT
TGAGGCTTGGTGGAATATAT

Downstream 100 bases:

>100_bases
CAGATATCGTTCTATGAGTATCGATTTCACTGTCTACAGTTTGAACGCCTGATGTAATACATCAGGCGTTTTTGAATGAT
ACAACGTCAAACTATGGCAA

Product: sulfate transporter

Products: Proton [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MQKKSLRYWMPGLTQLMAYERDWIKPDLRAGLSVAAVALPIAIAYAELTGVSAAVGLYSCILPMIAYAFFGSSRQLIVGP
DAATCAVIAAVVAPLAAGNSEVHWQLTIMMTLMMGSWCLVASRFKLGALADLLSRPILTGLLNGVAITIIVDQLGKVFGF
MTRPPQLIERVLALPYNMFNSHLPTVAISLLTFVVLYGVKWLRPNWPAPLLAIVIATFVSWAANMQQFGVDVVGGFEGGL
PIVHWPDFQPGLLRDMVIPALNLAVVSFVSMMLTARSFAAKNGYDVDADVELRALGITNIVSALSQGFAISGASTRTAVN
DANNGKSQLVSIIAALVIAMVLLFLTRPLQFIPIAALGVVLIYAAWSLLSFKSLWQLRKRNTQAFYLAIFTFVSVVLVGV
ISGIGLAVLLGLLQFLRTVFRPTEQLLGVNADGMIHSMGNGNGIKAVPGVMIYRFNSPLTYFNVAYFKRRILNLVDSTPH
PADWVVIDAVASFTYADISVLAAIDELKRDLKQRNIKLILAGRRTELTRWFRINRLKSHDDDLVLVPDLYLALKLIQSKR
RVAEKQEHESETGLEPEIG

Sequences:

>Translated_579_residues
MQKKSLRYWMPGLTQLMAYERDWIKPDLRAGLSVAAVALPIAIAYAELTGVSAAVGLYSCILPMIAYAFFGSSRQLIVGP
DAATCAVIAAVVAPLAAGNSEVHWQLTIMMTLMMGSWCLVASRFKLGALADLLSRPILTGLLNGVAITIIVDQLGKVFGF
MTRPPQLIERVLALPYNMFNSHLPTVAISLLTFVVLYGVKWLRPNWPAPLLAIVIATFVSWAANMQQFGVDVVGGFEGGL
PIVHWPDFQPGLLRDMVIPALNLAVVSFVSMMLTARSFAAKNGYDVDADVELRALGITNIVSALSQGFAISGASTRTAVN
DANNGKSQLVSIIAALVIAMVLLFLTRPLQFIPIAALGVVLIYAAWSLLSFKSLWQLRKRNTQAFYLAIFTFVSVVLVGV
ISGIGLAVLLGLLQFLRTVFRPTEQLLGVNADGMIHSMGNGNGIKAVPGVMIYRFNSPLTYFNVAYFKRRILNLVDSTPH
PADWVVIDAVASFTYADISVLAAIDELKRDLKQRNIKLILAGRRTELTRWFRINRLKSHDDDLVLVPDLYLALKLIQSKR
RVAEKQEHESETGLEPEIG
>Mature_579_residues
MQKKSLRYWMPGLTQLMAYERDWIKPDLRAGLSVAAVALPIAIAYAELTGVSAAVGLYSCILPMIAYAFFGSSRQLIVGP
DAATCAVIAAVVAPLAAGNSEVHWQLTIMMTLMMGSWCLVASRFKLGALADLLSRPILTGLLNGVAITIIVDQLGKVFGF
MTRPPQLIERVLALPYNMFNSHLPTVAISLLTFVVLYGVKWLRPNWPAPLLAIVIATFVSWAANMQQFGVDVVGGFEGGL
PIVHWPDFQPGLLRDMVIPALNLAVVSFVSMMLTARSFAAKNGYDVDADVELRALGITNIVSALSQGFAISGASTRTAVN
DANNGKSQLVSIIAALVIAMVLLFLTRPLQFIPIAALGVVLIYAAWSLLSFKSLWQLRKRNTQAFYLAIFTFVSVVLVGV
ISGIGLAVLLGLLQFLRTVFRPTEQLLGVNADGMIHSMGNGNGIKAVPGVMIYRFNSPLTYFNVAYFKRRILNLVDSTPH
PADWVVIDAVASFTYADISVLAAIDELKRDLKQRNIKLILAGRRTELTRWFRINRLKSHDDDLVLVPDLYLALKLIQSKR
RVAEKQEHESETGLEPEIG

Specific function: Expression in E.coli induces sulfate uptake during early-to mid-log phase growth. Uptake is maximal at pH 6.0, is sulfate-specific, requires E.coli CysA and the transmembrane segment but not the STAS domain of the protein [H]

COG id: COG0659

COG function: function code P; Sulfate permease and related transporters (MFS superfamily)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 STAS domain [H]

Homologues:

Organism=Homo sapiens, GI45827800, Length=489, Percent_Identity=27.8118609406953, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI39752683, Length=489, Percent_Identity=27.8118609406953, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI269784651, Length=488, Percent_Identity=27.0491803278689, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI45827802, Length=438, Percent_Identity=28.5388127853881, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI94721259, Length=477, Percent_Identity=29.1404612159329, Blast_Score=149, Evalue=1e-35,
Organism=Homo sapiens, GI94721257, Length=477, Percent_Identity=29.1404612159329, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI94721255, Length=477, Percent_Identity=29.1404612159329, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI94721253, Length=477, Percent_Identity=29.1404612159329, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI4505697, Length=513, Percent_Identity=27.2904483430799, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI4557535, Length=512, Percent_Identity=26.171875, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI16418413, Length=518, Percent_Identity=25.0965250965251, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI217272867, Length=510, Percent_Identity=25.2941176470588, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI262206105, Length=618, Percent_Identity=23.462783171521, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI262206075, Length=618, Percent_Identity=23.462783171521, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI262206069, Length=618, Percent_Identity=23.462783171521, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI262206063, Length=618, Percent_Identity=23.462783171521, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI47131207, Length=501, Percent_Identity=26.5469061876248, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI20336272, Length=501, Percent_Identity=26.5469061876248, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI100913030, Length=511, Percent_Identity=24.6575342465753, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI16418457, Length=502, Percent_Identity=25.8964143426295, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI301601599, Length=502, Percent_Identity=25.8964143426295, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI16306483, Length=622, Percent_Identity=20.5787781350482, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI20336282, Length=580, Percent_Identity=21.0344827586207, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI45827804, Length=258, Percent_Identity=27.5193798449612, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI301601602, Length=267, Percent_Identity=28.0898876404494, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI65506789, Length=380, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI87081859, Length=424, Percent_Identity=23.8207547169811, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17551690, Length=569, Percent_Identity=25.1318101933216, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI86564196, Length=543, Percent_Identity=25.414364640884, Blast_Score=144, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI86565215, Length=516, Percent_Identity=25.7751937984496, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17566848, Length=560, Percent_Identity=23.75, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17562578, Length=633, Percent_Identity=23.2227488151659, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI86564876, Length=441, Percent_Identity=26.0770975056689, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI193203292, Length=487, Percent_Identity=24.2299794661191, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI86565209, Length=234, Percent_Identity=26.9230769230769, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI86565211, Length=234, Percent_Identity=26.9230769230769, Blast_Score=69, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6325260, Length=569, Percent_Identity=23.1985940246046, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323121, Length=468, Percent_Identity=24.7863247863248, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319771, Length=451, Percent_Identity=25.9423503325942, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24649801, Length=578, Percent_Identity=24.9134948096886, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI21358229, Length=599, Percent_Identity=25.0417362270451, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24666186, Length=545, Percent_Identity=23.6697247706422, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI19922482, Length=542, Percent_Identity=25.830258302583, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI85815873, Length=457, Percent_Identity=24.507658643326, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24651449, Length=567, Percent_Identity=22.3985890652557, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24663084, Length=566, Percent_Identity=25.7950530035336, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21357695, Length=566, Percent_Identity=25.7950530035336, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21355087, Length=147, Percent_Identity=27.891156462585, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24647160, Length=147, Percent_Identity=27.891156462585, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002645
- InterPro:   IPR001902
- InterPro:   IPR011547 [H]

Pfam domain/function: PF01740 STAS; PF00916 Sulfate_transp [H]

EC number: NA

Molecular weight: Translated: 63342; Mature: 63342

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS50801 STAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKSLRYWMPGLTQLMAYERDWIKPDLRAGLSVAAVALPIAIAYAELTGVSAAVGLYSC
CCCCCCHHHCHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILPMIAYAFFGSSRQLIVGPDAATCAVIAAVVAPLAAGNSEVHWQLTIMMTLMMGSWCLV
HHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCHHHH
ASRFKLGALADLLSRPILTGLLNGVAITIIVDQLGKVFGFMTRPPQLIERVLALPYNMFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHH
SHLPTVAISLLTFVVLYGVKWLRPNWPAPLLAIVIATFVSWAANMQQFGVDVVGGFEGGL
CCHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCC
PIVHWPDFQPGLLRDMVIPALNLAVVSFVSMMLTARSFAAKNGYDVDADVELRALGITNI
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHH
VSALSQGFAISGASTRTAVNDANNGKSQLVSIIAALVIAMVLLFLTRPLQFIPIAALGVV
HHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LIYAAWSLLSFKSLWQLRKRNTQAFYLAIFTFVSVVLVGVISGIGLAVLLGLLQFLRTVF
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RPTEQLLGVNADGMIHSMGNGNGIKAVPGVMIYRFNSPLTYFNVAYFKRRILNLVDSTPH
CCHHHHHCCCCCCCEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCC
PADWVVIDAVASFTYADISVLAAIDELKRDLKQRNIKLILAGRRTELTRWFRINRLKSHD
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHCCCC
DDLVLVPDLYLALKLIQSKRRVAEKQEHESETGLEPEIG
CCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQKKSLRYWMPGLTQLMAYERDWIKPDLRAGLSVAAVALPIAIAYAELTGVSAAVGLYSC
CCCCCCHHHCHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILPMIAYAFFGSSRQLIVGPDAATCAVIAAVVAPLAAGNSEVHWQLTIMMTLMMGSWCLV
HHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCHHHH
ASRFKLGALADLLSRPILTGLLNGVAITIIVDQLGKVFGFMTRPPQLIERVLALPYNMFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHH
SHLPTVAISLLTFVVLYGVKWLRPNWPAPLLAIVIATFVSWAANMQQFGVDVVGGFEGGL
CCHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCC
PIVHWPDFQPGLLRDMVIPALNLAVVSFVSMMLTARSFAAKNGYDVDADVELRALGITNI
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHH
VSALSQGFAISGASTRTAVNDANNGKSQLVSIIAALVIAMVLLFLTRPLQFIPIAALGVV
HHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LIYAAWSLLSFKSLWQLRKRNTQAFYLAIFTFVSVVLVGVISGIGLAVLLGLLQFLRTVF
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RPTEQLLGVNADGMIHSMGNGNGIKAVPGVMIYRFNSPLTYFNVAYFKRRILNLVDSTPH
CCHHHHHCCCCCCCEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCC
PADWVVIDAVASFTYADISVLAAIDELKRDLKQRNIKLILAGRRTELTRWFRINRLKSHD
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHCCCC
DDLVLVPDLYLALKLIQSKRRVAEKQEHESETGLEPEIG
CCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; SO42- [Periplasm] [C]

Specific reaction: Proton [Periplasm] + SO42- [Periplasm] = Proton [Cytoplasm] + SO42- [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]