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The map label for this gene is ogt [H]

Identifier: 170024182

GI number: 170024182

Start: 2158863

End: 2159381

Strand: Reverse

Name: ogt [H]

Synonym: YPK_1945

Alternate gene names: 170024182

Gene position: 2159381-2158863 (Counterclockwise)

Preceding gene: 170024186

Following gene: 170024181

Centisome position: 46.05

GC content: 47.98

Gene sequence:

>519_bases
ATGAAAACGTTCTTTATTGATCGGATGGCGACCCCACAGGGCGAACTATTGCTTATTGCAGACGAAGAAAACCAGTTGCG
TGCCATTGATTGGACTGATCATCACGACCGCCTGATGAAATTACTACGTAACCATTACGGTGCGGATACGTTCACTTTGG
TTGAAAAACGCAACCCCGGTGGATTGAGCGAGAGTATGCAGCGCTATTTTGCGGGTGAATTAAGCATTATTGATACCCTT
CCCGTCAAAACAGCGGGTACCGACTTTCAGCGCCAAGTCTGGCAAGAACTACGCAAAATACCTTGTGGGGAAACTATTTC
CTATGGGGAACTGGCAAAACGCATTAATCGCCCCACGGCTTCTCGGGCAGTCGGTATGGCCAATGGGCTGAATCCTATTT
CTATTGTTGTTCCTTGCCATCGGGTGATTGGCTCACAAGGGGCATTGACTGGTTATGCGGGCGGAGTGGACCGAAAACGT
TGGTTATTAGTACATGAGGGTTATCTTTTAGGTTGCTAA

Upstream 100 bases:

>100_bases
TACCATACGAGCGGCCCATTAAGTGACCTAATGACTCGATGTTTTATGCAATAATAATTATGGAAAAAATTTAATCCGTG
AATAGCAACTATGGAGAGTT

Downstream 100 bases:

>100_bases
TCATTGTTGCTCTTATCAGAAGCACTAAAAGATGTTAAAATTGACGCATATCAATATTAATGGGAATACCCTATGATCCC
TGAAAAACGCGTTATCCGAC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MKTFFIDRMATPQGELLLIADEENQLRAIDWTDHHDRLMKLLRNHYGADTFTLVEKRNPGGLSESMQRYFAGELSIIDTL
PVKTAGTDFQRQVWQELRKIPCGETISYGELAKRINRPTASRAVGMANGLNPISIVVPCHRVIGSQGALTGYAGGVDRKR
WLLVHEGYLLGC

Sequences:

>Translated_172_residues
MKTFFIDRMATPQGELLLIADEENQLRAIDWTDHHDRLMKLLRNHYGADTFTLVEKRNPGGLSESMQRYFAGELSIIDTL
PVKTAGTDFQRQVWQELRKIPCGETISYGELAKRINRPTASRAVGMANGLNPISIVVPCHRVIGSQGALTGYAGGVDRKR
WLLVHEGYLLGC
>Mature_172_residues
MKTFFIDRMATPQGELLLIADEENQLRAIDWTDHHDRLMKLLRNHYGADTFTLVEKRNPGGLSESMQRYFAGELSIIDTL
PVKTAGTDFQRQVWQELRKIPCGETISYGELAKRINRPTASRAVGMANGLNPISIVVPCHRVIGSQGALTGYAGGVDRKR
WLLVHEGYLLGC

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=85, Percent_Identity=50.5882352941176, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1787596, Length=170, Percent_Identity=57.0588235294118, Blast_Score=212, Evalue=9e-57,
Organism=Escherichia coli, GI1788542, Length=89, Percent_Identity=53.9325842696629, Blast_Score=103, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI115533070, Length=93, Percent_Identity=47.3118279569892, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115533068, Length=93, Percent_Identity=47.3118279569892, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320001, Length=135, Percent_Identity=37.037037037037, Blast_Score=95, Evalue=6e-21,
Organism=Drosophila melanogaster, GI17137554, Length=99, Percent_Identity=40.4040404040404, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19283; Mature: 19283

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFFIDRMATPQGELLLIADEENQLRAIDWTDHHDRLMKLLRNHYGADTFTLVEKRNPG
CCEEEECCCCCCCCCEEEEECCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCC
GLSESMQRYFAGELSIIDTLPVKTAGTDFQRQVWQELRKIPCGETISYGELAKRINRPTA
CHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCH
SRAVGMANGLNPISIVVPCHRVIGSQGALTGYAGGVDRKRWLLVHEGYLLGC
HHHHHHCCCCCCEEEEEEHHHHHCCCCCCCCCCCCCCCCEEEEEECCEEECC
>Mature Secondary Structure
MKTFFIDRMATPQGELLLIADEENQLRAIDWTDHHDRLMKLLRNHYGADTFTLVEKRNPG
CCEEEECCCCCCCCCEEEEECCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCC
GLSESMQRYFAGELSIIDTLPVKTAGTDFQRQVWQELRKIPCGETISYGELAKRINRPTA
CHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCH
SRAVGMANGLNPISIVVPCHRVIGSQGALTGYAGGVDRKRWLLVHEGYLLGC
HHHHHHCCCCCCEEEEEEHHHHHCCCCCCCCCCCCCCCCEEEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2825131; 9097039; 9278503; 1520330 [H]