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The map label for this gene is tpx

Identifier: 170024142

GI number: 170024142

Start: 2109133

End: 2109636

Strand: Reverse

Name: tpx

Synonym: YPK_1903

Alternate gene names: 170024142

Gene position: 2109636-2109133 (Counterclockwise)

Preceding gene: 170024144

Following gene: 170024140

Centisome position: 44.99

GC content: 49.6

Gene sequence:

>504_bases
ATGACACAGACCGTACATTTCCAAGGCAATCCAGTTTCTGTCGCAGGTAAACTGCCACAAATTGGTGATAAAGCGAAAGA
CTTCACTTTGGTAGCAAAAGATTTATCTGACGTTGCCCTGAGCAGCTTTGCTGGCAAACGCAAAGTCCTGAATATCTTCC
CAAGTATTGATACCGGCGTTTGCGCCGCCTCGGTACGTAAGTTCAATCAACTGGCTGGCGAACTTGAGAACACCGTAGTG
CTTTGTATCTCCTCTGACCTGCCCTTTGCTCAATCACGTTTTTGCGGGGCTGAAGGTCTGAGCAACGTCATCACGCTGTC
AACTCTGCGCGGCGCTGATTTTAAACAAGCTTACGGTGTAGCAATTACTGAAGGCCCTCTGGCAGGTTTGACCGCACGCG
CGGTTGTCGTTCTGGATGGCCAGGACAACGTCATCTACAGCGAATTGGTTAACGAAATCACTACCGAACCAAACTACGAT
GCCGCGCTGGCTGCACTGAAATAA

Upstream 100 bases:

>100_bases
AAGTCTTTAAACGGGTTTTTTAGCGAACTTGCTATATCCAAGCTGTTGTTGTTATCTTAAACTGAAATTCGTGTTACACA
TCTGTAAAAGGGACCCAATA

Downstream 100 bases:

>100_bases
GTATCACCACCCAGCATTAATCAATGCCGTGTTGTAATCAATACCATGTTGCAATCAATGCCGTGTTGTAATCAATGCTG
TTTAGTCAATGCTGTATCGT

Product: thiol peroxidase

Products: oxidized thioredoxin; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MTQTVHFQGNPVSVAGKLPQIGDKAKDFTLVAKDLSDVALSSFAGKRKVLNIFPSIDTGVCAASVRKFNQLAGELENTVV
LCISSDLPFAQSRFCGAEGLSNVITLSTLRGADFKQAYGVAITEGPLAGLTARAVVVLDGQDNVIYSELVNEITTEPNYD
AALAALK

Sequences:

>Translated_167_residues
MTQTVHFQGNPVSVAGKLPQIGDKAKDFTLVAKDLSDVALSSFAGKRKVLNIFPSIDTGVCAASVRKFNQLAGELENTVV
LCISSDLPFAQSRFCGAEGLSNVITLSTLRGADFKQAYGVAITEGPLAGLTARAVVVLDGQDNVIYSELVNEITTEPNYD
AALAALK
>Mature_166_residues
TQTVHFQGNPVSVAGKLPQIGDKAKDFTLVAKDLSDVALSSFAGKRKVLNIFPSIDTGVCAASVRKFNQLAGELENTVVL
CISSDLPFAQSRFCGAEGLSNVITLSTLRGADFKQAYGVAITEGPLAGLTARAVVVLDGQDNVIYSELVNEITTEPNYDA
ALAALK

Specific function: Has antioxidant activity. Could remove peroxides or H(2)O(2)

COG id: COG2077

COG function: function code O; Peroxiredoxin

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain

Homologues:

Organism=Escherichia coli, GI1787584, Length=167, Percent_Identity=79.6407185628742, Blast_Score=276, Evalue=4e-76,

Paralogues:

None

Copy number: 2480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPX_YERPE (Q8ZE42)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AG0285
- RefSeq:   YP_002347309.1
- ProteinModelPortal:   Q8ZE42
- SMR:   Q8ZE42
- PeroxiBase:   6002
- GeneID:   1175174
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO2342
- KEGG:   ypk:y1990
- KEGG:   ypm:YP_2129
- HOGENOM:   HBG493509
- OMA:   VRKFNAQ
- ProtClustDB:   PRK00522
- BioCyc:   YPES187410:Y1990-MONOMER
- BioCyc:   YPES214092:YPO2342-MONOMER
- HAMAP:   MF_00269
- InterPro:   IPR018219
- InterPro:   IPR002065
- InterPro:   IPR013740
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335
- Gene3D:   G3DSA:3.40.30.10
- PANTHER:   PTHR10681:SF3

Pfam domain/function: PF08534 Redoxin; SSF52833 Thiordxn-like_fd

EC number: 1.11.1.- [C]

Molecular weight: Translated: 17592; Mature: 17461

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS51352 THIOREDOXIN_2; PS01265 TPX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTVHFQGNPVSVAGKLPQIGDKAKDFTLVAKDLSDVALSSFAGKRKVLNIFPSIDTGV
CCEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHH
CAASVRKFNQLAGELENTVVLCISSDLPFAQSRFCGAEGLSNVITLSTLRGADFKQAYGV
HHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHCCCCCCCCCEEEEEECCCCCCHHHCCE
AITEGPLAGLTARAVVVLDGQDNVIYSELVNEITTEPNYDAALAALK
EEECCCCCCCCEEEEEEEECCCCEEHHHHHHHHCCCCCCCHHEEECC
>Mature Secondary Structure 
TQTVHFQGNPVSVAGKLPQIGDKAKDFTLVAKDLSDVALSSFAGKRKVLNIFPSIDTGV
CEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHH
CAASVRKFNQLAGELENTVVLCISSDLPFAQSRFCGAEGLSNVITLSTLRGADFKQAYGV
HHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHCCCCCCCCCEEEEEECCCCCCHHHCCE
AITEGPLAGLTARAVVVLDGQDNVIYSELVNEITTEPNYDAALAALK
EEECCCCCCCCEEEEEEEECCCCEEHHHHHHHHCCCCCCCHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced thioredoxin; H2O2 [C]

Specific reaction: reduced thioredoxin + H2O2 = oxidized thioredoxin + H2O [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430