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The map label for this gene is purR [H]

Identifier: 170024101

GI number: 170024101

Start: 2069381

End: 2070406

Strand: Reverse

Name: purR [H]

Synonym: YPK_1862

Alternate gene names: 170024101

Gene position: 2070406-2069381 (Counterclockwise)

Preceding gene: 170024102

Following gene: 170024100

Centisome position: 44.15

GC content: 49.9

Gene sequence:

>1026_bases
ATGGCAACGATTAAAGATGTGGCCAAACACGCAGGTGTGTCCACAACCACCGTTTCGCACGTTATCAACAAAACTCGTTT
CGTCGCCGAAAATACGAAGGCTGCCGTGTGGGCCGCCATTAAAGAGCTGCATTATTCACCCAGTGCTGTCGCCCGCAGTT
TGAAAGTGAATCACACCAAGTCGATTGGTTTGCTGGCAACGTCCAGTGAAGCGCCCTATTTTGCTGAGGTGATCGAAGCG
GTAGAAAATAGCTGCTATAGCAAAGGTTACACGCTGATTTTATGTAATTCTCATAATAATCTGGATAAACAAAAAGCCTA
TCTGGCGATGTTGGCACAAAAGCGTGTCGATGGTTTATTGGTGATGTGCTCCGAATATCCAGATCAACTGCTGGGGATGC
TAGAGGATTATCGCAATATTCCTATGGTCGTGATGGACTGGGGGACTGCCCGTGGCGATTTTACTGACTCTATCATTGAT
AACGCCTTCGAAGGGGGCTATTTAGCGGGCCGTTATCTGATTGAACGCGGGCATCGTGATATTGGTGCGATACCCGGCCA
GTTGGCGCGTAATACCGGCGGTGGCCGTCATCAAGGCTTCCTTAAAGCCTTGGAAGAAGCCAATATCCCTGTTCGTGAAG
AGTGGATTGTTCAGGGCGATTTTGAGCCAGAATCCGGTTATAAAGCTATGCATCAAATTCTGACACAAAAACATCGCCCA
ACGGCGGTGTTCTGTGGCGGCGATATCATGGCGATGGGCGCAATCTGTGCCGCCGACGAACTGGGCCTACGGGTACCACA
AGATATTTCGGTGATCGGGTATGATAATGTGCGTAACGCGCGCTACTTCTCACCAGCACTGACCACTATCCATCAACCTA
AAGAGAGATTAGGCGAAACCGCCTTTGCCATGTTGCTGGACCGCATTGTCAGTAAGCGTGAAGATCCGCAGACCATAGAG
GTACATCCTAAACTGGTGGAGCGTCGTTCCGTTGCTGATGGCCCTTTCCGTGATTATCGCCGTTGA

Upstream 100 bases:

>100_bases
ACTATCTTGTTTCTGTCTGAGTGGTTTGCGGGTAACGCAGGCAAACGATTAACATGATACTGACCTGTTTCAGCCGGTCA
GTTTAGGAGTCATTCTTTTT

Downstream 100 bases:

>100_bases
TGTTTGCCTTGGCCGGTCGTATTCAGCACTGCCGGCCCGATTCGGATAACCGCATTCCCTGCGGTTATTTGCTATCGATG
GTTGGCTATCAATTGTTTGC

Product: DNA-binding transcriptional repressor PurR

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MATIKDVAKHAGVSTTTVSHVINKTRFVAENTKAAVWAAIKELHYSPSAVARSLKVNHTKSIGLLATSSEAPYFAEVIEA
VENSCYSKGYTLILCNSHNNLDKQKAYLAMLAQKRVDGLLVMCSEYPDQLLGMLEDYRNIPMVVMDWGTARGDFTDSIID
NAFEGGYLAGRYLIERGHRDIGAIPGQLARNTGGGRHQGFLKALEEANIPVREEWIVQGDFEPESGYKAMHQILTQKHRP
TAVFCGGDIMAMGAICAADELGLRVPQDISVIGYDNVRNARYFSPALTTIHQPKERLGETAFAMLLDRIVSKREDPQTIE
VHPKLVERRSVADGPFRDYRR

Sequences:

>Translated_341_residues
MATIKDVAKHAGVSTTTVSHVINKTRFVAENTKAAVWAAIKELHYSPSAVARSLKVNHTKSIGLLATSSEAPYFAEVIEA
VENSCYSKGYTLILCNSHNNLDKQKAYLAMLAQKRVDGLLVMCSEYPDQLLGMLEDYRNIPMVVMDWGTARGDFTDSIID
NAFEGGYLAGRYLIERGHRDIGAIPGQLARNTGGGRHQGFLKALEEANIPVREEWIVQGDFEPESGYKAMHQILTQKHRP
TAVFCGGDIMAMGAICAADELGLRVPQDISVIGYDNVRNARYFSPALTTIHQPKERLGETAFAMLLDRIVSKREDPQTIE
VHPKLVERRSVADGPFRDYRR
>Mature_340_residues
ATIKDVAKHAGVSTTTVSHVINKTRFVAENTKAAVWAAIKELHYSPSAVARSLKVNHTKSIGLLATSSEAPYFAEVIEAV
ENSCYSKGYTLILCNSHNNLDKQKAYLAMLAQKRVDGLLVMCSEYPDQLLGMLEDYRNIPMVVMDWGTARGDFTDSIIDN
AFEGGYLAGRYLIERGHRDIGAIPGQLARNTGGGRHQGFLKALEEANIPVREEWIVQGDFEPESGYKAMHQILTQKHRPT
AVFCGGDIMAMGAICAADELGLRVPQDISVIGYDNVRNARYFSPALTTIHQPKERLGETAFAMLLDRIVSKREDPQTIEV
HPKLVERRSVADGPFRDYRR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=341, Percent_Identity=82.6979472140763, Blast_Score=605, Evalue=1e-174,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=47.2727272727273, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI1790369, Length=309, Percent_Identity=35.2750809061489, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1789202, Length=333, Percent_Identity=34.2342342342342, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1787580, Length=326, Percent_Identity=31.9018404907975, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1788474, Length=332, Percent_Identity=30.7228915662651, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1786540, Length=310, Percent_Identity=33.5483870967742, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789068, Length=315, Percent_Identity=28.8888888888889, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1789456, Length=338, Percent_Identity=28.9940828402367, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI48994940, Length=320, Percent_Identity=26.875, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787906, Length=254, Percent_Identity=25.9842519685039, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790715, Length=327, Percent_Identity=23.8532110091743, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786268, Length=279, Percent_Identity=25.4480286738351, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790689, Length=320, Percent_Identity=24.6875, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790192, Length=245, Percent_Identity=25.7142857142857, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37840; Mature: 37708

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAKHAGVSTTTVSHVINKTRFVAENTKAAVWAAIKELHYSPSAVARSLKVNHTK
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCC
SIGLLATSSEAPYFAEVIEAVENSCYSKGYTLILCNSHNNLDKQKAYLAMLAQKRVDGLL
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCEE
VMCSEYPDQLLGMLEDYRNIPMVVMDWGTARGDFTDSIIDNAFEGGYLAGRYLIERGHRD
EEHHHCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCC
IGAIPGQLARNTGGGRHQGFLKALEEANIPVREEWIVQGDFEPESGYKAMHQILTQKHRP
CCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCC
TAVFCGGDIMAMGAICAADELGLRVPQDISVIGYDNVRNARYFSPALTTIHQPKERLGET
CEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHCHHHHHHHH
AFAMLLDRIVSKREDPQTIEVHPKLVERRSVADGPFRDYRR
HHHHHHHHHHHCCCCCCEEEECHHHHHHHCCCCCCHHHCCC
>Mature Secondary Structure 
ATIKDVAKHAGVSTTTVSHVINKTRFVAENTKAAVWAAIKELHYSPSAVARSLKVNHTK
CCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCC
SIGLLATSSEAPYFAEVIEAVENSCYSKGYTLILCNSHNNLDKQKAYLAMLAQKRVDGLL
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCEE
VMCSEYPDQLLGMLEDYRNIPMVVMDWGTARGDFTDSIIDNAFEGGYLAGRYLIERGHRD
EEHHHCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCC
IGAIPGQLARNTGGGRHQGFLKALEEANIPVREEWIVQGDFEPESGYKAMHQILTQKHRP
CCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCC
TAVFCGGDIMAMGAICAADELGLRVPQDISVIGYDNVRNARYFSPALTTIHQPKERLGET
CEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHCHHHHHHHH
AFAMLLDRIVSKREDPQTIEVHPKLVERRSVADGPFRDYRR
HHHHHHHHHHHCCCCCCEEEECHHHHHHHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA