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The map label for this gene is rluE [H]

Identifier: 170023963

GI number: 170023963

Start: 1909969

End: 1910595

Strand: Reverse

Name: rluE [H]

Synonym: YPK_1722

Alternate gene names: 170023963

Gene position: 1910595-1909969 (Counterclockwise)

Preceding gene: 170023965

Following gene: 170023962

Centisome position: 40.74

GC content: 49.44

Gene sequence:

>627_bases
ATGAATAAATTCTCTGTTAAAAATCACAAAGTTAACCGATTCAGCACTAAGGCACAAGCCAGGCAGCCCCCCCCCGCTGC
GCCACGGCGGGTATTGTTATTCAATAAACCTTTTGATGTATTACCGCAATTCACTGATGAGTCTGGACGCACCACTTTGC
AGGGTTTCATCCCTTTTAAGGACGTTTATGCTGCTGGCCGGTTAGATCGCGATAGCGAGGGCTTATTGGTATTAACCAAT
GATGGCAAGTTGCAAGCCAGGCTCACGCAACCGGCGCAAAAAACAGGTAAAGTCTATTTTGTACAAGTAGAAGGGGTCCC
TGACAACGATGCGCTCACACAGCTAAAACGAGGCGTAACGTTGAAAGATGGCCTGACGTTACCCGCAGGCGCGGAGTTAG
TGGCAGAGCCTGAGTGGCTATGGCCGCGCCATCCCCCTATCAGAGAGCGTAAAACCATCCCGACCAGTTGGTTGAAAATA
ACACTTTATGAAGGCCGCAACCGGCAAGTACGTAGAATGACCGCACACGTTGGCTTCCCTACCCTGCGCCTGATTCGTTT
TAGTATGGGAAATTTAAGCCTTGGCAACTTACAGCCCGGCGAATGGAAGGAGATAACTGATGTTTAA

Upstream 100 bases:

>100_bases
GCTTTGTAAGATTCGTGTCAATACTACTTGAATATTCGTTCCGCGCCAATCCGAAACGGATTGGGGTATAATGCGCTAAT
CATTCGAAACCGCAATAATC

Downstream 100 bases:

>100_bases
ACCGCATGTTACTGTCGCCTGTGTCGTTCATACTCAGGGGAAATTTCTGATCGTTGAAGAAACCATCAACAATGAAAAAC
GCTGGAACCAGCCAGCCGGT

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MNKFSVKNHKVNRFSTKAQARQPPPAAPRRVLLFNKPFDVLPQFTDESGRTTLQGFIPFKDVYAAGRLDRDSEGLLVLTN
DGKLQARLTQPAQKTGKVYFVQVEGVPDNDALTQLKRGVTLKDGLTLPAGAELVAEPEWLWPRHPPIRERKTIPTSWLKI
TLYEGRNRQVRRMTAHVGFPTLRLIRFSMGNLSLGNLQPGEWKEITDV

Sequences:

>Translated_208_residues
MNKFSVKNHKVNRFSTKAQARQPPPAAPRRVLLFNKPFDVLPQFTDESGRTTLQGFIPFKDVYAAGRLDRDSEGLLVLTN
DGKLQARLTQPAQKTGKVYFVQVEGVPDNDALTQLKRGVTLKDGLTLPAGAELVAEPEWLWPRHPPIRERKTIPTSWLKI
TLYEGRNRQVRRMTAHVGFPTLRLIRFSMGNLSLGNLQPGEWKEITDV
>Mature_208_residues
MNKFSVKNHKVNRFSTKAQARQPPPAAPRRVLLFNKPFDVLPQFTDESGRTTLQGFIPFKDVYAAGRLDRDSEGLLVLTN
DGKLQARLTQPAQKTGKVYFVQVEGVPDNDALTQLKRGVTLKDGLTLPAGAELVAEPEWLWPRHPPIRERKTIPTSWLKI
TLYEGRNRQVRRMTAHVGFPTLRLIRFSMGNLSLGNLQPGEWKEITDV

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=207, Percent_Identity=73.4299516908213, Blast_Score=322, Evalue=1e-89,
Organism=Escherichia coli, GI1787524, Length=187, Percent_Identity=36.3636363636364, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788510, Length=184, Percent_Identity=32.6086956521739, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1790453, Length=191, Percent_Identity=30.3664921465969, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 23553; Mature: 23553

Theoretical pI: Translated: 10.92; Mature: 10.92

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKFSVKNHKVNRFSTKAQARQPPPAAPRRVLLFNKPFDVLPQFTDESGRTTLQGFIPFK
CCCCCCCCCCCCHHHCHHHCCCCCCCCCCEEEEECCCHHHCCCCCCCCCCEEEEECCCHH
DVYAAGRLDRDSEGLLVLTNDGKLQARLTQPAQKTGKVYFVQVEGVPDNDALTQLKRGVT
HHHHHCCCCCCCCCEEEEECCCEEEEEECCCHHHCCCEEEEEECCCCCCHHHHHHHCCCE
LKDGLTLPAGAELVAEPEWLWPRHPPIRERKTIPTSWLKITLYEGRNRQVRRMTAHVGFP
ECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCC
TLRLIRFSMGNLSLGNLQPGEWKEITDV
CEEEEEECCCCEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MNKFSVKNHKVNRFSTKAQARQPPPAAPRRVLLFNKPFDVLPQFTDESGRTTLQGFIPFK
CCCCCCCCCCCCHHHCHHHCCCCCCCCCCEEEEECCCHHHCCCCCCCCCCEEEEECCCHH
DVYAAGRLDRDSEGLLVLTNDGKLQARLTQPAQKTGKVYFVQVEGVPDNDALTQLKRGVT
HHHHHCCCCCCCCCEEEEECCCEEEEEECCCHHHCCCEEEEEECCCCCCHHHHHHHCCCE
LKDGLTLPAGAELVAEPEWLWPRHPPIRERKTIPTSWLKITLYEGRNRQVRRMTAHVGFP
ECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCC
TLRLIRFSMGNLSLGNLQPGEWKEITDV
CEEEEEECCCCEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]