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The map label for this gene is htrB [H]

Identifier: 170023906

GI number: 170023906

Start: 1851258

End: 1852178

Strand: Reverse

Name: htrB [H]

Synonym: YPK_1665

Alternate gene names: 170023906

Gene position: 1852178-1851258 (Counterclockwise)

Preceding gene: 170023911

Following gene: 170023905

Centisome position: 39.5

GC content: 50.27

Gene sequence:

>921_bases
ATGCAAGCTCCTCCTTTTGATCGTTCACTGCTGCACCCACGTTATTGGCTGACCTGGATCGGCCTCGGTATTCTTTACCT
GCTGGTGCTGCTCCCCTACCTGGTTATCTATGCGATGGGAACATCACTGGGTCGTTTTTCTATGCACTTTCTTAAACGAC
GTAAGCGGATCACACGGCGTAATTTGGAGCTGTGTTTTCCCCATATGTCACCAGAGGAACGTGAGGCGCTGCTGGTCAAG
AACTTTGAATCCGTGGGGATGGGGCTATTTGAAACCGGTATGGCGTGGTTTTGGCCAAATTGGCGTATTGAGCGTTGGAG
CCGTGTCAGTGGTCTGGAACATATTCAACAGGCCCGCGATGAAGGTAAAGGTGTTTTGCTGATCGGGTTACATTTTCTCA
CGCTAGAACTGGGCGCACGTATCTTTGGTATCTACAATCCAGGCATCGGCGTTTATCGCCCCCATGATAATAAAGCAATG
GACTGGATGCAGACATGGGGACGGATGCGTTCGAATAAGGCCATGTTGGACCGCAAAGATCTGAAAGGGATGATCCGTAG
CCTAAAACAAGGTGACATTATCTGGTACGCGCCAGATCATGATTATGGCCCGCGTAGCAGTGTCTTTGTCCCTCTGTTTG
CCGTCGATCAAGCCGCGACCACCACTGGCACTTATTTGCTGGTCCGGATGGGTAAACCCGCCGTTATTCCCTTTACCCCA
CGCCGTTTACCTGACGCCAAGGGATACCAACTGATTTTGCAACCCGCAGTCGAAAACTTCCCGTTAGATAATGAGGTCGA
AGCTGCGGCTTTTATGAACAAAGTCATAGAGAAAGAGATTGCCCTCGCTACTGACCAATATATGTGGCTGCACCGTCGCT
TTAAGACCCGGCCAGAAGGGGAACCTTCTTTATATGAGTAA

Upstream 100 bases:

>100_bases
CAGACTTCTCGTCAAAAAAATATTTTGATCAATAAACCCCTTCATCAATACGGAAACGATTAGCAGTATTAATTAATCAC
AATGACGCTGAGAACTGATA

Downstream 100 bases:

>100_bases
AGATATTAATACCTTACTCCTCGGTGTTGCCACGCTCATGATCCGTCACGTTTTACTGATGTGTCACGTTTAGGGAAATT
GGGTGACAGAATCGGCCCGA

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MQAPPFDRSLLHPRYWLTWIGLGILYLLVLLPYLVIYAMGTSLGRFSMHFLKRRKRITRRNLELCFPHMSPEEREALLVK
NFESVGMGLFETGMAWFWPNWRIERWSRVSGLEHIQQARDEGKGVLLIGLHFLTLELGARIFGIYNPGIGVYRPHDNKAM
DWMQTWGRMRSNKAMLDRKDLKGMIRSLKQGDIIWYAPDHDYGPRSSVFVPLFAVDQAATTTGTYLLVRMGKPAVIPFTP
RRLPDAKGYQLILQPAVENFPLDNEVEAAAFMNKVIEKEIALATDQYMWLHRRFKTRPEGEPSLYE

Sequences:

>Translated_306_residues
MQAPPFDRSLLHPRYWLTWIGLGILYLLVLLPYLVIYAMGTSLGRFSMHFLKRRKRITRRNLELCFPHMSPEEREALLVK
NFESVGMGLFETGMAWFWPNWRIERWSRVSGLEHIQQARDEGKGVLLIGLHFLTLELGARIFGIYNPGIGVYRPHDNKAM
DWMQTWGRMRSNKAMLDRKDLKGMIRSLKQGDIIWYAPDHDYGPRSSVFVPLFAVDQAATTTGTYLLVRMGKPAVIPFTP
RRLPDAKGYQLILQPAVENFPLDNEVEAAAFMNKVIEKEIALATDQYMWLHRRFKTRPEGEPSLYE
>Mature_306_residues
MQAPPFDRSLLHPRYWLTWIGLGILYLLVLLPYLVIYAMGTSLGRFSMHFLKRRKRITRRNLELCFPHMSPEEREALLVK
NFESVGMGLFETGMAWFWPNWRIERWSRVSGLEHIQQARDEGKGVLLIGLHFLTLELGARIFGIYNPGIGVYRPHDNKAM
DWMQTWGRMRSNKAMLDRKDLKGMIRSLKQGDIIWYAPDHDYGPRSSVFVPLFAVDQAATTTGTYLLVRMGKPAVIPFTP
RRLPDAKGYQLILQPAVENFPLDNEVEAAAFMNKVIEKEIALATDQYMWLHRRFKTRPEGEPSLYE

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=302, Percent_Identity=66.2251655629139, Blast_Score=440, Evalue=1e-125,
Organism=Escherichia coli, GI87082094, Length=300, Percent_Identity=53.6666666666667, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1788161, Length=307, Percent_Identity=28.0130293159609, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35572; Mature: 35572

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAPPFDRSLLHPRYWLTWIGLGILYLLVLLPYLVIYAMGTSLGRFSMHFLKRRKRITRR
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
NLELCFPHMSPEEREALLVKNFESVGMGLFETGMAWFWPNWRIERWSRVSGLEHIQQARD
CHHEEECCCCCHHHHHHHHHCHHHHCCCHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHH
EGKGVLLIGLHFLTLELGARIFGIYNPGIGVYRPHDNKAMDWMQTWGRMRSNKAMLDRKD
CCCCEEEEHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
LKGMIRSLKQGDIIWYAPDHDYGPRSSVFVPLFAVDQAATTTGTYLLVRMGKPAVIPFTP
HHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCEEECCCC
RRLPDAKGYQLILQPAVENFPLDNEVEAAAFMNKVIEKEIALATDQYMWLHRRFKTRPEG
CCCCCCCCCEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EPSLYE
CCCCCC
>Mature Secondary Structure
MQAPPFDRSLLHPRYWLTWIGLGILYLLVLLPYLVIYAMGTSLGRFSMHFLKRRKRITRR
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
NLELCFPHMSPEEREALLVKNFESVGMGLFETGMAWFWPNWRIERWSRVSGLEHIQQARD
CHHEEECCCCCHHHHHHHHHCHHHHCCCHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHH
EGKGVLLIGLHFLTLELGARIFGIYNPGIGVYRPHDNKAMDWMQTWGRMRSNKAMLDRKD
CCCCEEEEHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
LKGMIRSLKQGDIIWYAPDHDYGPRSSVFVPLFAVDQAATTTGTYLLVRMGKPAVIPFTP
HHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCEEECCCC
RRLPDAKGYQLILQPAVENFPLDNEVEAAAFMNKVIEKEIALATDQYMWLHRRFKTRPEG
CCCCCCCCCEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EPSLYE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]