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The map label for this gene is dmlA [H]

Identifier: 170023857

GI number: 170023857

Start: 1787273

End: 1788358

Strand: Direct

Name: dmlA [H]

Synonym: YPK_1616

Alternate gene names: 170023857

Gene position: 1787273-1788358 (Clockwise)

Preceding gene: 170023854

Following gene: 170023858

Centisome position: 38.11

GC content: 49.54

Gene sequence:

>1086_bases
ATGAAGAAAACCTACCGAATTGCCGCCATACCCGGGGACGGTATCGGCCATGAAGTGATGCCAGAAGGGATCAACGTGCT
CCAGGCCGCCGCTTTGCGCTGGGATTTAGATATTTCATTTGAAAATATTGAATGGGCAAGTTGTGAATATTATCAACACC
ATGGGCAGATGATGCCGAATGATTGGCAGAGGCAGCTACAATGCTTCGATGCGATTTATTTTGGTGCGGTTGGCTGGCCT
GCGACCGTGCCAGACCACATTTCGCTGTGGGGATCGCTGTTGAAGTTTCGTCGTGATTTTCAGCAGTATGTCAATTTACG
TCCGGTGCGCTTGTTTCCTGGCATACCCTGCCCGTTAGTGGGTAAACAGCCCGGTGATATTGATTTTTATGTGGTTCGGG
AAAATACCGAAGGTGAATATTCATCGGTTGGCGGGCGTTTGTTTGAAGGCACCGAGCAGGAAATGGTTTTACAGGCGTCG
ATATTCACACGCCGTGGCGTTGACCGTATTCTAAAATACGCCTTTGAATTGGCGCAGATGCGCCCACGTAAGCGCCTGAC
GTCTGCCACTAAATCTAATGGTCTGGCGTTCAGTATGCCGTACTGGGATGAGCGCTTCGAAGTGATGGCGGCGCAGTATC
CACAAATTAGCGCGGACAAACAGCATATTGATATTTTGTGCGCCCGTTTTGTATTGAATCCTGAACGTTTTGATGTGGTT
GTGGCATCTAACTTATTTGGTGATATTTTATCTGACCTTGGCCCGGCGTGTGCGGGGACGATTGGCATTGCGCCTTCAGC
GAACCTCAATCCAGAGCGTGACTTCCCTTCACTGTTCGAACCGGTACATGGCTCGGCCCCTGATATTTATAAAAGAAATA
TCGCTAACCCTATTGCGATGATCTGGGCGGGGGCGATGATGCTCGATTTTCTGGGGCAAGGAGATGAGCGCTATCATGCT
GCCCATAATGGAATATTAAACGCTATTGAACAGGTGATTGCGCAGGGCCCTAAAACGCCTGATATGTGTGGTGATGCCTC
CACCCAACAAGTGGGTTCTGCCATTGTGCAGGTCTTATCTCACTAA

Upstream 100 bases:

>100_bases
TCTGTGTTTTATGTATCACCAGTTTTTGTATTGTATGTAAATTAGTCAATTATAGGATGGAGGAGATGTCAATTCTTACC
GCTGATAAATGAGACTTGCG

Downstream 100 bases:

>100_bases
TCCTCCCGCCTATTTTATCGTCCTGGCCCCTTATTATCAGACTGCTGACAAACCCCGATGACGCGATCTTGAACGGTGAG
GATAGGCAGAGGAGTAAAGC

Product: tartrate dehydrogenase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKKTYRIAAIPGDGIGHEVMPEGINVLQAAALRWDLDISFENIEWASCEYYQHHGQMMPNDWQRQLQCFDAIYFGAVGWP
ATVPDHISLWGSLLKFRRDFQQYVNLRPVRLFPGIPCPLVGKQPGDIDFYVVRENTEGEYSSVGGRLFEGTEQEMVLQAS
IFTRRGVDRILKYAFELAQMRPRKRLTSATKSNGLAFSMPYWDERFEVMAAQYPQISADKQHIDILCARFVLNPERFDVV
VASNLFGDILSDLGPACAGTIGIAPSANLNPERDFPSLFEPVHGSAPDIYKRNIANPIAMIWAGAMMLDFLGQGDERYHA
AHNGILNAIEQVIAQGPKTPDMCGDASTQQVGSAIVQVLSH

Sequences:

>Translated_361_residues
MKKTYRIAAIPGDGIGHEVMPEGINVLQAAALRWDLDISFENIEWASCEYYQHHGQMMPNDWQRQLQCFDAIYFGAVGWP
ATVPDHISLWGSLLKFRRDFQQYVNLRPVRLFPGIPCPLVGKQPGDIDFYVVRENTEGEYSSVGGRLFEGTEQEMVLQAS
IFTRRGVDRILKYAFELAQMRPRKRLTSATKSNGLAFSMPYWDERFEVMAAQYPQISADKQHIDILCARFVLNPERFDVV
VASNLFGDILSDLGPACAGTIGIAPSANLNPERDFPSLFEPVHGSAPDIYKRNIANPIAMIWAGAMMLDFLGQGDERYHA
AHNGILNAIEQVIAQGPKTPDMCGDASTQQVGSAIVQVLSH
>Mature_361_residues
MKKTYRIAAIPGDGIGHEVMPEGINVLQAAALRWDLDISFENIEWASCEYYQHHGQMMPNDWQRQLQCFDAIYFGAVGWP
ATVPDHISLWGSLLKFRRDFQQYVNLRPVRLFPGIPCPLVGKQPGDIDFYVVRENTEGEYSSVGGRLFEGTEQEMVLQAS
IFTRRGVDRILKYAFELAQMRPRKRLTSATKSNGLAFSMPYWDERFEVMAAQYPQISADKQHIDILCARFVLNPERFDVV
VASNLFGDILSDLGPACAGTIGIAPSANLNPERDFPSLFEPVHGSAPDIYKRNIANPIAMIWAGAMMLDFLGQGDERYHA
AHNGILNAIEQVIAQGPKTPDMCGDASTQQVGSAIVQVLSH

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=356, Percent_Identity=30.6179775280899, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI4758582, Length=366, Percent_Identity=29.2349726775956, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI28178816, Length=367, Percent_Identity=27.2479564032698, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI28178838, Length=315, Percent_Identity=30.1587301587302, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI28178821, Length=326, Percent_Identity=27.6073619631902, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI28178819, Length=163, Percent_Identity=33.1288343558282, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1788101, Length=359, Percent_Identity=81.3370473537605, Blast_Score=618, Evalue=1e-178,
Organism=Escherichia coli, GI87081683, Length=372, Percent_Identity=36.5591397849462, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1787381, Length=350, Percent_Identity=26.8571428571429, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17550882, Length=358, Percent_Identity=31.0055865921788, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71986051, Length=348, Percent_Identity=31.3218390804598, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17505779, Length=355, Percent_Identity=27.0422535211268, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI25144293, Length=318, Percent_Identity=26.4150943396226, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322097, Length=366, Percent_Identity=36.6120218579235, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6324709, Length=363, Percent_Identity=31.129476584022, Blast_Score=151, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6319830, Length=366, Percent_Identity=32.2404371584699, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324291, Length=367, Percent_Identity=29.4277929155313, Blast_Score=123, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24643270, Length=310, Percent_Identity=31.9354838709677, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24643268, Length=310, Percent_Identity=31.9354838709677, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24661184, Length=360, Percent_Identity=29.7222222222222, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI161078639, Length=269, Percent_Identity=31.9702602230483, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078637, Length=269, Percent_Identity=31.9702602230483, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078635, Length=269, Percent_Identity=31.9702602230483, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078633, Length=269, Percent_Identity=31.9702602230483, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24650122, Length=269, Percent_Identity=31.9702602230483, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281362242, Length=357, Percent_Identity=28.5714285714286, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24648872, Length=357, Percent_Identity=28.5714285714286, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI20130355, Length=372, Percent_Identity=22.3118279569892, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.83 [H]

Molecular weight: Translated: 40340; Mature: 40340

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTYRIAAIPGDGIGHEVMPEGINVLQAAALRWDLDISFENIEWASCEYYQHHGQMMPN
CCCEEEEEECCCCCCCCHHCHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHCCCCCCH
DWQRQLQCFDAIYFGAVGWPATVPDHISLWGSLLKFRRDFQQYVNLRPVRLFPGIPCPLV
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
GKQPGDIDFYVVRENTEGEYSSVGGRLFEGTEQEMVLQASIFTRRGVDRILKYAFELAQM
CCCCCCEEEEEEECCCCCCHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RPRKRLTSATKSNGLAFSMPYWDERFEVMAAQYPQISADKQHIDILCARFVLNPERFDVV
CHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHEEE
VASNLFGDILSDLGPACAGTIGIAPSANLNPERDFPSLFEPVHGSAPDIYKRNIANPIAM
EHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHCCCHHHH
IWAGAMMLDFLGQGDERYHAAHNGILNAIEQVIAQGPKTPDMCGDASTQQVGSAIVQVLS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHC
H
C
>Mature Secondary Structure
MKKTYRIAAIPGDGIGHEVMPEGINVLQAAALRWDLDISFENIEWASCEYYQHHGQMMPN
CCCEEEEEECCCCCCCCHHCHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHCCCCCCH
DWQRQLQCFDAIYFGAVGWPATVPDHISLWGSLLKFRRDFQQYVNLRPVRLFPGIPCPLV
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
GKQPGDIDFYVVRENTEGEYSSVGGRLFEGTEQEMVLQASIFTRRGVDRILKYAFELAQM
CCCCCCEEEEEEECCCCCCHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RPRKRLTSATKSNGLAFSMPYWDERFEVMAAQYPQISADKQHIDILCARFVLNPERFDVV
CHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHEEE
VASNLFGDILSDLGPACAGTIGIAPSANLNPERDFPSLFEPVHGSAPDIYKRNIANPIAM
EHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHCCCHHHH
IWAGAMMLDFLGQGDERYHAAHNGILNAIEQVIAQGPKTPDMCGDASTQQVGSAIVQVLS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHC
H
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]