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The map label for this gene is rlmF

Identifier: 170023837

GI number: 170023837

Start: 1764969

End: 1765934

Strand: Direct

Name: rlmF

Synonym: YPK_1596

Alternate gene names: 170023837

Gene position: 1764969-1765934 (Clockwise)

Preceding gene: 170023835

Following gene: 170023838

Centisome position: 37.64

GC content: 49.38

Gene sequence:

>966_bases
ATGGAAAATAAAAAAGTATTCCCCAAAGAAAAAAGCGGATTGCATCCCCGAAACCGTCATCGCTCACGCTATGATTTTGA
TGCGCTTTCAGTGAGTTGCCCTGAGCTAATCCCTTTTTTGGCCCCGACCGCCTATGGTGATATTTCTGTCGATTTTGCTG
ATCCATTAGCAGTAAAAATGTTGAATAAGGCATTATTAAAACATTTCTATGGCATTGAATATTGGGATATTCCAGCCGAT
TCTTTATGTCCTCCCATTCCTGGGCGTGCCGATTACGTGCACCATCTGGCCGATCTATTAGCCAGTTGCAATGGCGAGGT
GATCCCTAAGGGCAAGAATATTGCGTTGCTGGATATTGGCGTCGGCGCTAACTGTATTTATCCAATTATTGGTCAGCGGG
AGTACGGCTGGCGCTTTACCGGTACGGATATTGACTCACACGCGCTCAGTGCGGCAAAAATGGTGGTGTCGATGAATCCG
ACCCTGAAAAATACCTTGCGCCTGAAGCAGCAAAAAGATCCACACGCTATTTTTGAAGGGGTGTGGGCGGTGAATGAGCG
TTACGATGCGACGCTGTGTAACCCGCCATTCCATGGTTCAGCAGAAGAAGCGGCGGCCACCACTCGCCGTAAATTGCACA
AACTGGGGAAAAATGAAGTCGCTGCAAAGCCAGTACAGAACTTCGGTGGTAAAAACAGTGAGTTATGGTGTGAAGGTGGG
GAGGAAGGGTTTGTCAGCCGCATGGTGGCAGAGAGCGTAGCGAAAGCGCAGAACTGCTTCTGGTTTACCTCATTGATTTC
CAAGAAGACCACGTTACCGGCGATTTATCATGCGTTAAGATATGTGAAGGCAGTTGAGGTGCGCACTATTGAGATGGCGC
AAGGGCAAAAAGTCAGTCGGTTTGTGGCCTGGACGTTCCTGACGCCCGAGCAGCAAGCCGCGTGGGTTGCAGAGCGCTGG
GCATAA

Upstream 100 bases:

>100_bases
CAAAGGGGCTGGTGGTGTAGAACCTATTCACCGTACACCCGTTTAGCCGTAATTTATGCTATCTTACGCGCCAGAAAATG
CCTATCAGAGAGCCAGTACG

Downstream 100 bases:

>100_bases
CGTTTCCACTATATAACCAAATAAGCCAGTGACCACGACAAGTCAGTGATCACGATCAACCCGTTACCGTGGTTAACGAA
CCAGCATGGCGCAACGCTAT

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MENKKVFPKEKSGLHPRNRHRSRYDFDALSVSCPELIPFLAPTAYGDISVDFADPLAVKMLNKALLKHFYGIEYWDIPAD
SLCPPIPGRADYVHHLADLLASCNGEVIPKGKNIALLDIGVGANCIYPIIGQREYGWRFTGTDIDSHALSAAKMVVSMNP
TLKNTLRLKQQKDPHAIFEGVWAVNERYDATLCNPPFHGSAEEAAATTRRKLHKLGKNEVAAKPVQNFGGKNSELWCEGG
EEGFVSRMVAESVAKAQNCFWFTSLISKKTTLPAIYHALRYVKAVEVRTIEMAQGQKVSRFVAWTFLTPEQQAAWVAERW
A

Sequences:

>Translated_321_residues
MENKKVFPKEKSGLHPRNRHRSRYDFDALSVSCPELIPFLAPTAYGDISVDFADPLAVKMLNKALLKHFYGIEYWDIPAD
SLCPPIPGRADYVHHLADLLASCNGEVIPKGKNIALLDIGVGANCIYPIIGQREYGWRFTGTDIDSHALSAAKMVVSMNP
TLKNTLRLKQQKDPHAIFEGVWAVNERYDATLCNPPFHGSAEEAAATTRRKLHKLGKNEVAAKPVQNFGGKNSELWCEGG
EEGFVSRMVAESVAKAQNCFWFTSLISKKTTLPAIYHALRYVKAVEVRTIEMAQGQKVSRFVAWTFLTPEQQAAWVAERW
A
>Mature_321_residues
MENKKVFPKEKSGLHPRNRHRSRYDFDALSVSCPELIPFLAPTAYGDISVDFADPLAVKMLNKALLKHFYGIEYWDIPAD
SLCPPIPGRADYVHHLADLLASCNGEVIPKGKNIALLDIGVGANCIYPIIGQREYGWRFTGTDIDSHALSAAKMVVSMNP
TLKNTLRLKQQKDPHAIFEGVWAVNERYDATLCNPPFHGSAEEAAATTRRKLHKLGKNEVAAKPVQNFGGKNSELWCEGG
EEGFVSRMVAESVAKAQNCFWFTSLISKKTTLPAIYHALRYVKAVEVRTIEMAQGQKVSRFVAWTFLTPEQQAAWVAERW
A

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family

Homologues:

Organism=Homo sapiens, GI122114654, Length=296, Percent_Identity=29.7297297297297, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI87081786, Length=307, Percent_Identity=57.6547231270358, Blast_Score=358, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32564931, Length=318, Percent_Identity=27.9874213836478, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32564933, Length=318, Percent_Identity=25.4716981132075, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI281363415, Length=308, Percent_Identity=28.2467532467532, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI19922302, Length=308, Percent_Identity=28.2467532467532, Blast_Score=125, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMF_YERP3 (A7FGU1)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001400469.1
- ProteinModelPortal:   A7FGU1
- SMR:   A7FGU1
- STRING:   A7FGU1
- GeneID:   5387444
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_1491
- eggNOG:   COG3129
- HOGENOM:   HBG747880
- OMA:   AELWCEG
- ProtClustDB:   PRK11727
- BioCyc:   YPSE349747:YPSIP31758_1491-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01848
- InterPro:   IPR016909
- InterPro:   IPR010286
- PANTHER:   PTHR13393
- PIRSF:   PIRSF029038

Pfam domain/function: PF05971 Methyltransf_10

EC number: =2.1.1.181

Molecular weight: Translated: 35846; Mature: 35846

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKKVFPKEKSGLHPRNRHRSRYDFDALSVSCPELIPFLAPTAYGDISVDFADPLAVKM
CCCCCCCCCCCCCCCCCHHCCCCCCCHHHCCCCHHHHHHHCCCCCCCEEECCCCHHHHHH
LNKALLKHFYGIEYWDIPADSLCPPIPGRADYVHHLADLLASCNGEVIPKGKNIALLDIG
HHHHHHHHHCCCCEECCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEC
VGANCIYPIIGQREYGWRFTGTDIDSHALSAAKMVVSMNPTLKNTLRLKQQKDPHAIFEG
CCCCEEECCCCCCCCCCEEECCCCCHHHHHHHHHEEECCCCHHHHHHHHCCCCHHHHHHH
VWAVNERYDATLCNPPFHGSAEEAAATTRRKLHKLGKNEVAAKPVQNFGGKNSELWCEGG
HHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCHHHHCCCCCCEEEECCC
EEGFVSRMVAESVAKAQNCFWFTSLISKKTTLPAIYHALRYVKAVEVRTIEMAQGQKVSR
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEHHCCCCHHH
FVAWTFLTPEQQAAWVAERWA
EEEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure
MENKKVFPKEKSGLHPRNRHRSRYDFDALSVSCPELIPFLAPTAYGDISVDFADPLAVKM
CCCCCCCCCCCCCCCCCHHCCCCCCCHHHCCCCHHHHHHHCCCCCCCEEECCCCHHHHHH
LNKALLKHFYGIEYWDIPADSLCPPIPGRADYVHHLADLLASCNGEVIPKGKNIALLDIG
HHHHHHHHHCCCCEECCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEC
VGANCIYPIIGQREYGWRFTGTDIDSHALSAAKMVVSMNPTLKNTLRLKQQKDPHAIFEG
CCCCEEECCCCCCCCCCEEECCCCCHHHHHHHHHEEECCCCHHHHHHHHCCCCHHHHHHH
VWAVNERYDATLCNPPFHGSAEEAAATTRRKLHKLGKNEVAAKPVQNFGGKNSELWCEGG
HHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCHHHHCCCCCCEEEECCC
EEGFVSRMVAESVAKAQNCFWFTSLISKKTTLPAIYHALRYVKAVEVRTIEMAQGQKVSR
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEHHCCCCHHH
FVAWTFLTPEQQAAWVAERWA
EEEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA