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The map label for this gene is menE [H]

Identifier: 170023834

GI number: 170023834

Start: 1761554

End: 1762969

Strand: Direct

Name: menE [H]

Synonym: YPK_1593

Alternate gene names: 170023834

Gene position: 1761554-1762969 (Clockwise)

Preceding gene: 170023833

Following gene: 170023835

Centisome position: 37.56

GC content: 57.7

Gene sequence:

>1416_bases
ATGGCATCGTTAGGCCATCCGCCTTGGGAGCATTGGCCTTGGCAGTATTGGGCCAATCTGCGGCCTCAAGCGCTCGCTAT
ACGTTCTGGGGGGCAACGGATCAGTTGGCAACAGTTAGCCAGTGATATCAATGTGTTGGCGGCCTCTTTTCAACAGCAAG
GTGTGGTGCCAGGGAGCGGCGTGGCCTTGCGCGGTAAAAACAGTTATACCCTGCTATTGGCTTATCTGGCGGCATTGCAG
TGTGCGGCTCGCGTTTTGCCGCTGAATCCGCAATTACCGGATGCGCTTTTGGCGCAGCGCCTGCCAACGCTGGATATTCA
CTTTATGCTGAATTTAGCCACCCCGTTGCCCCCGCATTTTGCCTTTACTCCGTTGGATCCCCGCACCGTTGATTCGGCAG
CCGTGACCCCGATAGCGTGGGATGGCCAACGGCTGGCGACGCTGACGCTAACCTCAGGTTCCTCCGGCTTACCGAAGGCC
GCAGTACACTCGCTGGGCGCACATCGGGCTAATGCCGACGGCGTACTGGCGCTGATGAATTTCACCGCCAGTGATAGCTG
GTTGCTGTCGTTACCGTTGTTTCATGTCTCAGGGCAGGGGATTGTCTGGCGTTGGCTGACGGCGGGGGCGACACTGGGGG
TACCAGAGGGCGTGCCACTGGTTGAGGCTTTGGCGGGGTGCAGCCATGCGTCATTAGTTCCCACGCAATTATGGCGTTTG
TTGGCGAATGCCGCCCCCCCCTTGACGTTGAAAGAGGTACTGTTGGGGGGCGCGATGATCCCAACCGAACTGACCGAACA
GGCCGAGGCCCACGGCATCCGCTGTTGGTGTGGCTACGGCTTGACGGAGTTGGCATCAACGGTCTGTGCCAAGCGGGCAG
ACGGGCGACCTGGTGTCGGTGTCGCGCTCGCAGGCCGTCAGGTGAAACTGGTAGACGATGAAGTCTGGATAAAAGCCGAT
TGTCTGGCTGCCGGTTACTGGCAGCAGGGGCAATTACTGCCACTCACGGACAGCGACGGGTGGTTCCATACCCGAGATCG
CGGTAGGTGGCAGCAAGGCGAATTATGTATTCTCGGGCGGTTGGATAATCTGTTTTTCAGTGGTGGCGAGGGGATCCAGC
CAGAAGACATCGAGCGAGTTTTATTGCAGTATTCAGGGGTGCAGCACGCTTTTGTTATTCCTGTTGCTGACGCTGAATTC
GGTCACCGGCCAGTGGCGGTCATTGATTCCGCTGATGAGGTGGATGTCGTGGCTTTAGCTGGTTGGTTAGCCCCACGATT
GGCTGTCTTCCAGCGGCCCATTGCTTTTTACCGGCTACCCACATTTTTGAAGCGTGGTGGGATTAAGATCTCAAGGCAGC
AACTGGCCGACTGGCTGCGCGGCAAGTATGAAGGGGAGAAGATTGGCGAAATATAG

Upstream 100 bases:

>100_bases
TTCCGGGGTTGGATACGCTGGACCTGATGCAGGCGCAAGTCGTGCGGCCGTGGCCAGACAGCCCGTTGCCGCTAATCACG
ACCGAGCAGTTGGGCGTGGT

Downstream 100 bases:

>100_bases
AGCATGTCAGGCAAACTCAGCCCAGTTTATTGTTAACGAGAGACCCATAGGGGAAAGGCCGCACCATGGATTACAGTAAA
ATTATCAAAGAGGTTGGGCG

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MASLGHPPWEHWPWQYWANLRPQALAIRSGGQRISWQQLASDINVLAASFQQQGVVPGSGVALRGKNSYTLLLAYLAALQ
CAARVLPLNPQLPDALLAQRLPTLDIHFMLNLATPLPPHFAFTPLDPRTVDSAAVTPIAWDGQRLATLTLTSGSSGLPKA
AVHSLGAHRANADGVLALMNFTASDSWLLSLPLFHVSGQGIVWRWLTAGATLGVPEGVPLVEALAGCSHASLVPTQLWRL
LANAAPPLTLKEVLLGGAMIPTELTEQAEAHGIRCWCGYGLTELASTVCAKRADGRPGVGVALAGRQVKLVDDEVWIKAD
CLAAGYWQQGQLLPLTDSDGWFHTRDRGRWQQGELCILGRLDNLFFSGGEGIQPEDIERVLLQYSGVQHAFVIPVADAEF
GHRPVAVIDSADEVDVVALAGWLAPRLAVFQRPIAFYRLPTFLKRGGIKISRQQLADWLRGKYEGEKIGEI

Sequences:

>Translated_471_residues
MASLGHPPWEHWPWQYWANLRPQALAIRSGGQRISWQQLASDINVLAASFQQQGVVPGSGVALRGKNSYTLLLAYLAALQ
CAARVLPLNPQLPDALLAQRLPTLDIHFMLNLATPLPPHFAFTPLDPRTVDSAAVTPIAWDGQRLATLTLTSGSSGLPKA
AVHSLGAHRANADGVLALMNFTASDSWLLSLPLFHVSGQGIVWRWLTAGATLGVPEGVPLVEALAGCSHASLVPTQLWRL
LANAAPPLTLKEVLLGGAMIPTELTEQAEAHGIRCWCGYGLTELASTVCAKRADGRPGVGVALAGRQVKLVDDEVWIKAD
CLAAGYWQQGQLLPLTDSDGWFHTRDRGRWQQGELCILGRLDNLFFSGGEGIQPEDIERVLLQYSGVQHAFVIPVADAEF
GHRPVAVIDSADEVDVVALAGWLAPRLAVFQRPIAFYRLPTFLKRGGIKISRQQLADWLRGKYEGEKIGEI
>Mature_470_residues
ASLGHPPWEHWPWQYWANLRPQALAIRSGGQRISWQQLASDINVLAASFQQQGVVPGSGVALRGKNSYTLLLAYLAALQC
AARVLPLNPQLPDALLAQRLPTLDIHFMLNLATPLPPHFAFTPLDPRTVDSAAVTPIAWDGQRLATLTLTSGSSGLPKAA
VHSLGAHRANADGVLALMNFTASDSWLLSLPLFHVSGQGIVWRWLTAGATLGVPEGVPLVEALAGCSHASLVPTQLWRLL
ANAAPPLTLKEVLLGGAMIPTELTEQAEAHGIRCWCGYGLTELASTVCAKRADGRPGVGVALAGRQVKLVDDEVWIKADC
LAAGYWQQGQLLPLTDSDGWFHTRDRGRWQQGELCILGRLDNLFFSGGEGIQPEDIERVLLQYSGVQHAFVIPVADAEFG
HRPVAVIDSADEVDVVALAGWLAPRLAVFQRPIAFYRLPTFLKRGGIKISRQQLADWLRGKYEGEKIGEI

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=451, Percent_Identity=55.2106430155211, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI145693145, Length=451, Percent_Identity=23.7250554323725, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1788107, Length=363, Percent_Identity=26.4462809917355, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=356, Percent_Identity=29.2134831460674, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI32563687, Length=450, Percent_Identity=21.7777777777778, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 51044; Mature: 50913

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLGHPPWEHWPWQYWANLRPQALAIRSGGQRISWQQLASDINVLAASFQQQGVVPGSG
CCCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
VALRGKNSYTLLLAYLAALQCAARVLPLNPQLPDALLAQRLPTLDIHFMLNLATPLPPHF
EEEECCCCHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHCCCCEEEEEEEEECCCCCCCC
AFTPLDPRTVDSAAVTPIAWDGQRLATLTLTSGSSGLPKAAVHSLGAHRANADGVLALMN
EECCCCCCCCCCCCCCEEEECCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCEEEEEE
FTASDSWLLSLPLFHVSGQGIVWRWLTAGATLGVPEGVPLVEALAGCSHASLVPTQLWRL
ECCCCCEEEEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHH
LANAAPPLTLKEVLLGGAMIPTELTEQAEAHGIRCWCGYGLTELASTVCAKRADGRPGVG
HHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCCE
VALAGRQVKLVDDEVWIKADCLAAGYWQQGQLLPLTDSDGWFHTRDRGRWQQGELCILGR
EEECCCEEEEECCCEEEEEEEEEECCCCCCCEEEEECCCCCEEECCCCCCCCCCEEEEEE
LDNLFFSGGEGIQPEDIERVLLQYSGVQHAFVIPVADAEFGHRPVAVIDSADEVDVVALA
CCHHEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEECCCCCCCHHHHH
GWLAPRLAVFQRPIAFYRLPTFLKRGGIKISRQQLADWLRGKYEGEKIGEI
HHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ASLGHPPWEHWPWQYWANLRPQALAIRSGGQRISWQQLASDINVLAASFQQQGVVPGSG
CCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
VALRGKNSYTLLLAYLAALQCAARVLPLNPQLPDALLAQRLPTLDIHFMLNLATPLPPHF
EEEECCCCHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHCCCCEEEEEEEEECCCCCCCC
AFTPLDPRTVDSAAVTPIAWDGQRLATLTLTSGSSGLPKAAVHSLGAHRANADGVLALMN
EECCCCCCCCCCCCCCEEEECCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCEEEEEE
FTASDSWLLSLPLFHVSGQGIVWRWLTAGATLGVPEGVPLVEALAGCSHASLVPTQLWRL
ECCCCCEEEEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHH
LANAAPPLTLKEVLLGGAMIPTELTEQAEAHGIRCWCGYGLTELASTVCAKRADGRPGVG
HHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCCE
VALAGRQVKLVDDEVWIKADCLAAGYWQQGQLLPLTDSDGWFHTRDRGRWQQGELCILGR
EEECCCEEEEECCCEEEEEEEEEECCCCCCCEEEEECCCCCEEECCCCCCCCCCEEEEEE
LDNLFFSGGEGIQPEDIERVLLQYSGVQHAFVIPVADAEFGHRPVAVIDSADEVDVVALA
CCHHEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEECCCCCCCHHHHH
GWLAPRLAVFQRPIAFYRLPTFLKRGGIKISRQQLADWLRGKYEGEKIGEI
HHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]