Click here to switch to the map view.

The map label for this gene is mltB [H]

Identifier: 170023705

GI number: 170023705

Start: 1616059

End: 1617153

Strand: Direct

Name: mltB [H]

Synonym: YPK_1464

Alternate gene names: 170023705

Gene position: 1616059-1617153 (Clockwise)

Preceding gene: 170023701

Following gene: 170023706

Centisome position: 34.46

GC content: 52.05

Gene sequence:

>1095_bases
ATGCGTTATTTGGCTGTCATTCTTCCTTTGTTTACGTTGCTCTCAGCTTGTAGTAGCCAGCCGACGCCTCCTGTTGCTGC
AACCCCGTCAGATAGCCCTGTTCAGGGCGGTTTTTTACTTGAGCCGGAACATGCGGGCCAGTTGCAAAACGGTGATTTTG
CCTTTAACCCAGATACCACGCGTTTTGTTGACAAAATGGTGCGTGAGCACGGTTTTGATCGCCAGCAACTGCACGATGTA
TTGGCTCAGACCCAGCGGCTGGACTGGGTTATTCGCTTGATGGATAAGCAAGCGCCGACGTCCCGACCACCTTCCGGGCC
AAACGGTGCCTGGAATCGCTATCGCAATAAATTCATTACTCCTGATAATGTGCAGAACGGTGTGGTTTTCTGGAATCAAT
ATGAAGGTGCGCTGCAACGTGCCTGGGAGGTGTACGGTGTGCCGCCGGAAATTATCGTCGGGATCATCGGGGTTGAAACG
CGTTGGGGCCGGGTGATGGGGAAAACCCGAATCATCGATGCTTTGGCGACGCTGTCCTTTGCTTACCCACGGCGTGCAAC
ATTCTTTACGGGTGAGTTGGAAACCTTCTTATTAATGGCGCGTGCTGAGGGGAAAGACCCACTCAGTCTGCGGGGCTCCT
ATGCGGGGGCGATGGGCTATGGTCAATTTATGCCATCATCATTTAAGCGCTATGCGGTTGATTTTGATGGCAACGGCCAC
ATCAACTTATGGGATCCCGTTGATGCTATCGGCAGTGTTGCCAATTACTTCAAATCCCACGGTTGGACGAAAGGTGCGCC
GGTAGCTGTACCTGCCAATGGACAGGTTCCAAATCTCGAAAATGGATTTAAGACCCGTTATCCAATCTCGACTTTAGCGG
CAGCAGGGCTTAGCCCCAAAGGGCCGTTAGGCGATTATCAGGAGGCCAGTTTGCTGCGCTTTGATGTGGGTACCGGTTAT
CAATATTGGTACGGTTTGCCTAACTTCTACACGATTACCCGCTATAACCACAGTACCCATTATGCGATGGCGGTATGGCA
GTTAGGGGAAGCCGTGGGCAGAGTGCGCAAAGGGGAGTCAATGATTACCCAATAG

Upstream 100 bases:

>100_bases
TATATGAAGAGTACCGGCTTTAACGGCATTTTCCTGTTTGTTCACAACAGACAATGGCTTACCATAGCTATCACTTATTT
GTATGGTTTATAGGTATTCG

Downstream 100 bases:

>100_bases
CATGAAAAAGAGGGCCACATTAGGGTTATGCTGTCCTCTTTTCTTAACGATTTTTACTCATCCATCAGATGTGAAATCTC
ATCAGGATGCACTGAGGGTG

Product: murein hydrolase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MRYLAVILPLFTLLSACSSQPTPPVAATPSDSPVQGGFLLEPEHAGQLQNGDFAFNPDTTRFVDKMVREHGFDRQQLHDV
LAQTQRLDWVIRLMDKQAPTSRPPSGPNGAWNRYRNKFITPDNVQNGVVFWNQYEGALQRAWEVYGVPPEIIVGIIGVET
RWGRVMGKTRIIDALATLSFAYPRRATFFTGELETFLLMARAEGKDPLSLRGSYAGAMGYGQFMPSSFKRYAVDFDGNGH
INLWDPVDAIGSVANYFKSHGWTKGAPVAVPANGQVPNLENGFKTRYPISTLAAAGLSPKGPLGDYQEASLLRFDVGTGY
QYWYGLPNFYTITRYNHSTHYAMAVWQLGEAVGRVRKGESMITQ

Sequences:

>Translated_364_residues
MRYLAVILPLFTLLSACSSQPTPPVAATPSDSPVQGGFLLEPEHAGQLQNGDFAFNPDTTRFVDKMVREHGFDRQQLHDV
LAQTQRLDWVIRLMDKQAPTSRPPSGPNGAWNRYRNKFITPDNVQNGVVFWNQYEGALQRAWEVYGVPPEIIVGIIGVET
RWGRVMGKTRIIDALATLSFAYPRRATFFTGELETFLLMARAEGKDPLSLRGSYAGAMGYGQFMPSSFKRYAVDFDGNGH
INLWDPVDAIGSVANYFKSHGWTKGAPVAVPANGQVPNLENGFKTRYPISTLAAAGLSPKGPLGDYQEASLLRFDVGTGY
QYWYGLPNFYTITRYNHSTHYAMAVWQLGEAVGRVRKGESMITQ
>Mature_364_residues
MRYLAVILPLFTLLSACSSQPTPPVAATPSDSPVQGGFLLEPEHAGQLQNGDFAFNPDTTRFVDKMVREHGFDRQQLHDV
LAQTQRLDWVIRLMDKQAPTSRPPSGPNGAWNRYRNKFITPDNVQNGVVFWNQYEGALQRAWEVYGVPPEIIVGIIGVET
RWGRVMGKTRIIDALATLSFAYPRRATFFTGELETFLLMARAEGKDPLSLRGSYAGAMGYGQFMPSSFKRYAVDFDGNGH
INLWDPVDAIGSVANYFKSHGWTKGAPVAVPANGQVPNLENGFKTRYPISTLAAAGLSPKGPLGDYQEASLLRFDVGTGY
QYWYGLPNFYTITRYNHSTHYAMAVWQLGEAVGRVRKGESMITQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=357, Percent_Identity=75.0700280112045, Blast_Score=558, Evalue=1e-160,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 40483; Mature: 40483

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYLAVILPLFTLLSACSSQPTPPVAATPSDSPVQGGFLLEPEHAGQLQNGDFAFNPDTT
CHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCEEECCHHCCCCCCCCEEECCCHH
RFVDKMVREHGFDRQQLHDVLAQTQRLDWVIRLMDKQAPTSRPPSGPNGAWNRYRNKFIT
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCC
PDNVQNGVVFWNQYEGALQRAWEVYGVPPEIIVGIIGVETRWGRVMGKTRIIDALATLSF
CCCCCCCEEEEECHHHHHHHHHHHCCCCHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHH
AYPRRATFFTGELETFLLMARAEGKDPLSLRGSYAGAMGYGQFMPSSFKRYAVDFDGNGH
CCCCCCEEEECCHHHHHEEHHCCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEEECCCCC
INLWDPVDAIGSVANYFKSHGWTKGAPVAVPANGQVPNLENGFKTRYPISTLAAAGLSPK
EEECCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCC
GPLGDYQEASLLRFDVGTGYQYWYGLPNFYTITRYNHSTHYAMAVWQLGEAVGRVRKGES
CCCCCCCCCEEEEEECCCCCCEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCHH
MITQ
HCCC
>Mature Secondary Structure
MRYLAVILPLFTLLSACSSQPTPPVAATPSDSPVQGGFLLEPEHAGQLQNGDFAFNPDTT
CHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCEEECCHHCCCCCCCCEEECCCHH
RFVDKMVREHGFDRQQLHDVLAQTQRLDWVIRLMDKQAPTSRPPSGPNGAWNRYRNKFIT
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCC
PDNVQNGVVFWNQYEGALQRAWEVYGVPPEIIVGIIGVETRWGRVMGKTRIIDALATLSF
CCCCCCCEEEEECHHHHHHHHHHHCCCCHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHH
AYPRRATFFTGELETFLLMARAEGKDPLSLRGSYAGAMGYGQFMPSSFKRYAVDFDGNGH
CCCCCCEEEECCHHHHHEEHHCCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEEECCCCC
INLWDPVDAIGSVANYFKSHGWTKGAPVAVPANGQVPNLENGFKTRYPISTLAAAGLSPK
EEECCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCC
GPLGDYQEASLLRFDVGTGYQYWYGLPNFYTITRYNHSTHYAMAVWQLGEAVGRVRKGES
CCCCCCCCCEEEEEECCCCCCEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCHH
MITQ
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]