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The map label for this gene is gltX [H]

Identifier: 170023678

GI number: 170023678

Start: 1586063

End: 1587478

Strand: Reverse

Name: gltX [H]

Synonym: YPK_1437

Alternate gene names: 170023678

Gene position: 1587478-1586063 (Counterclockwise)

Preceding gene: 170023679

Following gene: 170023677

Centisome position: 33.85

GC content: 49.29

Gene sequence:

>1416_bases
ATGAAAATCAAAACCCGTTTTGCACCTAGTCCTACCGGCTATCTCCACGTGGGTGGCGCGCGTACTGCGCTCTACTCCTG
GCTATTCAGCCGCCATTTAGGTGGCGAATTTGTGCTGCGTATTGAAGACACCGATCTTGAACGCTCTACACAGGAAGCTA
TCGATGCCATTATGGACGGTATGAACTGGCTGAATCTGGATTGGGATGAAGGCCCGTATTTCCAAACCAAGCGTTTTGAT
CGCTATAACGCCGTTATTGACCAAATGCTTGATGCCGGTACAGCTTATCGCTGCTATTGCTCTAAAGAACGCCTGGAAGC
ATTGCGTGAAGCTCAAATGGCTAACGGAGAAAAGCCCCGCTACGATGGTCACTGCCGTGACAGCCAATGTACTCATGGCG
CTGATGAGCCGTCAGTGGTGCGTTTCCGTAACCCGCAGGAAGGCTCTGTTATTTTCGATGATAAAATTCGCGGGCCAATC
GAATTCAGCAATCAGGAACTGGATGACTTGATCATCCGCAGAACCGATGGTTCCCCAACCTATAATTTCTGCGTGGTGAT
TGATGACTGGGATATGGAGATCACCCATGTTATCCGTGGTGAAGACCATATTAACAACACACCTCGCCAGATTAATATTC
TAAAAGCATTAGGTGCACCAGTACCTGAGTATGCTCACGTCTCAATGATTTTGGGCGATGATGGCAAAAAGCTGTCTAAA
CGCCACGGGGCTGTTGGCGTCATGCAATACCGTGATGACGGGTACTTACCAGAAGCGTTGTTGAACTATTTAGTCCGTTT
AGGGTGGTCCCATGGCGACCAAGAGATTTTCTCTATTGAAGAGATGACCCAGTTATTTACCCTGGATGCCGTCAGTAAAT
CAGCCAGTGCCTTTAATACCGAAAAACTTCAGTGGCTGAATCATCACTATATCAATAGCCTGCCACCTGAACAGGTTGCC
GTACACCTTTCATGGCATGTTGAACAACTGGGTATTGATACACGTAATGGCCCTGAACTCGTTGAGATAGTAAAACTGCT
GGGCGAACGTTGTAAAACACTGAAAGAAATGGCGGAGTCCTGCCGTTACTTCTATGAAGAGTTTGACGCATTTGATGTAG
ATGCCGCTAAGAAGCACCTGCGCCCCGTTGCCCGCCAGCCTTTGGAAGCCGTTAAAGTTAAACTCGCCGCCATCACCGAA
TGGACCACAGAGAACGTCCACAACGCCATTCAGGGCACCGCGGATGAGTTAGGTGTCGGTATGGGTAAAGTGGGTATGCC
ACTGCGTGTTGCCGTGACAGGCGTTGGTCAATCACCCGGCATGGATGTGACTGTTCATGCTATCGGTCAAGCGCGCACGC
TGGCACGTATTGATAAAGCTCTGGCCTTTATCAGCGAACGCGAAGCTCAACAGTAA

Upstream 100 bases:

>100_bases
AGGAATTACCACACAGTGGTAGAATGTCGCCTACTTTTTAGAATTTCGAACAACTGTCAGCCTCTGCGGGCAGGCGTAGC
GTAATAAGGCATCAATCCCA

Downstream 100 bases:

>100_bases
AATTTATCATTTTCCGTGTTATCAGGCGGCCACTCTGGTCGCCTTTTTTATATTTGATAACAGCAGCGGTGTGACAACAA
ATTTGTGAACATCATTCCAT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFSRHLGGEFVLRIEDTDLERSTQEAIDAIMDGMNWLNLDWDEGPYFQTKRFD
RYNAVIDQMLDAGTAYRCYCSKERLEALREAQMANGEKPRYDGHCRDSQCTHGADEPSVVRFRNPQEGSVIFDDKIRGPI
EFSNQELDDLIIRRTDGSPTYNFCVVIDDWDMEITHVIRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGKKLSK
RHGAVGVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFSIEEMTQLFTLDAVSKSASAFNTEKLQWLNHHYINSLPPEQVA
VHLSWHVEQLGIDTRNGPELVEIVKLLGERCKTLKEMAESCRYFYEEFDAFDVDAAKKHLRPVARQPLEAVKVKLAAITE
WTTENVHNAIQGTADELGVGMGKVGMPLRVAVTGVGQSPGMDVTVHAIGQARTLARIDKALAFISEREAQQ

Sequences:

>Translated_471_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFSRHLGGEFVLRIEDTDLERSTQEAIDAIMDGMNWLNLDWDEGPYFQTKRFD
RYNAVIDQMLDAGTAYRCYCSKERLEALREAQMANGEKPRYDGHCRDSQCTHGADEPSVVRFRNPQEGSVIFDDKIRGPI
EFSNQELDDLIIRRTDGSPTYNFCVVIDDWDMEITHVIRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGKKLSK
RHGAVGVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFSIEEMTQLFTLDAVSKSASAFNTEKLQWLNHHYINSLPPEQVA
VHLSWHVEQLGIDTRNGPELVEIVKLLGERCKTLKEMAESCRYFYEEFDAFDVDAAKKHLRPVARQPLEAVKVKLAAITE
WTTENVHNAIQGTADELGVGMGKVGMPLRVAVTGVGQSPGMDVTVHAIGQARTLARIDKALAFISEREAQQ
>Mature_471_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFSRHLGGEFVLRIEDTDLERSTQEAIDAIMDGMNWLNLDWDEGPYFQTKRFD
RYNAVIDQMLDAGTAYRCYCSKERLEALREAQMANGEKPRYDGHCRDSQCTHGADEPSVVRFRNPQEGSVIFDDKIRGPI
EFSNQELDDLIIRRTDGSPTYNFCVVIDDWDMEITHVIRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGKKLSK
RHGAVGVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFSIEEMTQLFTLDAVSKSASAFNTEKLQWLNHHYINSLPPEQVA
VHLSWHVEQLGIDTRNGPELVEIVKLLGERCKTLKEMAESCRYFYEEFDAFDVDAAKKHLRPVARQPLEAVKVKLAAITE
WTTENVHNAIQGTADELGVGMGKVGMPLRVAVTGVGQSPGMDVTVHAIGQARTLARIDKALAFISEREAQQ

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=495, Percent_Identity=30.1010101010101, Blast_Score=221, Evalue=1e-57,
Organism=Escherichia coli, GI1788743, Length=471, Percent_Identity=84.5010615711253, Blast_Score=845, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=301, Percent_Identity=34.21926910299, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1786895, Length=119, Percent_Identity=26.0504201680672, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=321, Percent_Identity=38.9408099688474, Blast_Score=219, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6324540, Length=499, Percent_Identity=31.062124248497, Blast_Score=247, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6321192, Length=230, Percent_Identity=26.9565217391304, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324742, Length=213, Percent_Identity=24.8826291079812, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24665321, Length=491, Percent_Identity=30.5498981670061, Blast_Score=248, Evalue=9e-66,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 53169; Mature: 53169

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKTRFAPSPTGYLHVGGARTALYSWLFSRHLGGEFVLRIEDTDLERSTQEAIDAIMDG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHCC
MNWLNLDWDEGPYFQTKRFDRYNAVIDQMLDAGTAYRCYCSKERLEALREAQMANGEKPR
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHCCCCCCC
YDGHCRDSQCTHGADEPSVVRFRNPQEGSVIFDDKIRGPIEFSNQELDDLIIRRTDGSPT
CCCCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCCCCCHHEEEECCCCCC
YNFCVVIDDWDMEITHVIRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGKKLSK
EEEEEEEECCCCEEEEEECCCHHCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHH
RHGAVGVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFSIEEMTQLFTLDAVSKSASAFNT
HCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
EKLQWLNHHYINSLPPEQVAVHLSWHVEQLGIDTRNGPELVEIVKLLGERCKTLKEMAES
HHHHHHHHHHCCCCCHHHEEEEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
CRYFYEEFDAFDVDAAKKHLRPVARQPLEAVKVKLAAITEWTTENVHNAIQGTADELGVG
HHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
MGKVGMPLRVAVTGVGQSPGMDVTVHAIGQARTLARIDKALAFISEREAQQ
CCCCCCCEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIKTRFAPSPTGYLHVGGARTALYSWLFSRHLGGEFVLRIEDTDLERSTQEAIDAIMDG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHCC
MNWLNLDWDEGPYFQTKRFDRYNAVIDQMLDAGTAYRCYCSKERLEALREAQMANGEKPR
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHCCCCCCC
YDGHCRDSQCTHGADEPSVVRFRNPQEGSVIFDDKIRGPIEFSNQELDDLIIRRTDGSPT
CCCCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCCCCCHHEEEECCCCCC
YNFCVVIDDWDMEITHVIRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGKKLSK
EEEEEEEECCCCEEEEEECCCHHCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHH
RHGAVGVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFSIEEMTQLFTLDAVSKSASAFNT
HCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
EKLQWLNHHYINSLPPEQVAVHLSWHVEQLGIDTRNGPELVEIVKLLGERCKTLKEMAES
HHHHHHHHHHCCCCCHHHEEEEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
CRYFYEEFDAFDVDAAKKHLRPVARQPLEAVKVKLAAITEWTTENVHNAIQGTADELGVG
HHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
MGKVGMPLRVAVTGVGQSPGMDVTVHAIGQARTLARIDKALAFISEREAQQ
CCCCCCCEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA