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The map label for this gene is ligA [H]

Identifier: 170023674

GI number: 170023674

Start: 1581977

End: 1583989

Strand: Direct

Name: ligA [H]

Synonym: YPK_1432

Alternate gene names: 170023674

Gene position: 1581977-1583989 (Clockwise)

Preceding gene: 170023673

Following gene: 170023675

Centisome position: 33.73

GC content: 50.87

Gene sequence:

>2013_bases
ATGGAATCGATAATCCAACAAATTAATCAACTAAGAACTTCATTGCGCCATCATGAACATCAATACCATGTGCTGGATGC
CCCCGAAATCCCTGATGCTGAATACGACCGTATGATGCAACAACTGCGTGATTTAGAAGCACAACATCCTGAACTGGTGA
CTAATGACTCACCGACGCAACGGGTTGGTGCCGCGCCTCTTGATGCCTTTGAACAGGTGGAGCACGAAGTACCGATGCTG
TCTCTTGATAATGTGTTTGATGAAGAAAGTTATCTGGCCTTCGATAAACGGGTACATGACCGGCTTAAAACGGCAGAACC
ACTGACATTTTGTTGTGAACTGAAGCTAGATGGTCTGGCGGTCAGTTTATTGTATGAAAATGGTGAATTGGTGAGAGCCG
CGACACGTGGTGATGGCACTACGGGAGAAAATATTACCGCTAATGTGCGTACTATTCGTGCCATTCCACTGCGCCTACAT
GGGGATAATGTCCCGCGGCGCGTAGAAGTCCGTGGCGAGGTTTTTATGCCACAAGCCGGTTTTGAACAGCTTAACGAAGA
GGCTCGTCGCAAAGGAGGGAAGGTCTTTGCCAACCCACGGAATGCGGCGGCGGGATCGTTACGCCAACTTGATCCCCGTA
TTACAGCCAAACGGCCACTCACCTTCTTCTGCTATGGTGTCGGTCTGCTGGATGGCGGCGAGCTACCCCGTAGCCATATT
CAGTGTCTGATGCAATTTAAGGCATGGGGGTTACCCGTGAGTGAGCGGGTGAAACTCTGCACTGGCAGTGATCAGGTTAT
TGCTTTTTATCGTCAAATAGAGCAAGACCGCGCAGGCTTGGGCTTTGATATTGATGGTGTGGTGATCAAAGTTGATGATC
TTGTTTTGCAGGAGCAATTGGGGTTTGTTGCGCGAGCCCCACGCTGGGCAACGGCATTTAAATTCCCGGCGCAGGAACAG
ATCACACAGGTCCGTGAAGTGGAATTTCAAGTGGGTCGTACCGGTGCCATTACTCCCGTAGCCCGTCTGGAGCCGGTTCA
GGTGGCTGGCGTGATTGTCAGTAATGCGACATTGCATAATGCCGATGAAATCGAACGCCTTGGTTTGCGTATTGGCGATA
CGGTCATTGTGCGCCGTGCGGGTGATGTGATCCCACAAGTTGTTGGTGTGGTCATGGAACAACGCCCTGATGATACGAAG
GAGATCACTTTCCCAAGCCAATGCCCAGTGTGTGGCTCCGATATTGAACGGGTGGAAGGGGAGGCTGTTGCCCGCTGCAC
TGGCGGCTTGTTCTGTGCTGCACAGCGTAAAGAGGCACTTAAACATTTTGTCTCCCGGCGAGCACTGGATGTAGACGGTA
TGGGCGATAAAATTATCGAACAACTGGTCGAGAAACAGTATGTTGAAAATCCAGCTGATTTATTCCAACTGACCGCAGGT
AAGTTGACGGGGTTAGATCGGATGGGGCCAAAGTCTGCGCAAAATCTGATTGCGGCGCTGGAGAAGGCCAAACAGACCAC
CTTTGCACGCTTCTTGTATGCCTTGGGGATCCGCGAAGTGGGCGAAGCGACGGCCGCAAATTTAGCCGCTCACTTCCGCA
CTCTGGATAATCTGCGTGCAGCAGATATTGAGACGTTAAAAAGTGTGCCGGATGTCGGCGAGGTGGTGGCTAAACATGTG
ATGAACTTCCTCAGTGAGGAACATAATCAGAAAGTCATCGAAGAACTGGAGAAAGTGGTCAGTTGGCCTGAGCCGCAGCA
AATTGTAGTCGAGGAGATCGATAGCCCATTCGCTGGAAAAACGGTGGTCCTGACGGGGTCATTGACGATCCTTTCACGGG
ATGAAGCGAAAGACCGCCTGACAGCATTAGGCGCAAAAGTGAGTGGCAGTGTCTCTAAAAAAACGCATCTGGTGATCGCT
GGCGAGGCGGCTGGTTCTAAACTGGCGAAAGCGCAGGAACTCGGAATTAAAGTTATTGATGAAGCTGAAATGATCCGTTT
GCTGGGTGAATAA

Upstream 100 bases:

>100_bases
AATTGCTTGATAATCTTTTTTATAAAGCGATTGCCTCGTAATAAATCGAATTAACCCGAACCCCCGCATGCCGGGGGTTT
TTTATCTTTGGTGATGAGCT

Downstream 100 bases:

>100_bases
TCCTAACGCCGGGGGCGATATGGAAAAAGAGAATTTGATTGAGATTGCTAATACCCGAATGCCTTTTGGTAAATACCAAG
GGCGGGTGTTGATCGACTTG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MESIIQQINQLRTSLRHHEHQYHVLDAPEIPDAEYDRMMQQLRDLEAQHPELVTNDSPTQRVGAAPLDAFEQVEHEVPML
SLDNVFDEESYLAFDKRVHDRLKTAEPLTFCCELKLDGLAVSLLYENGELVRAATRGDGTTGENITANVRTIRAIPLRLH
GDNVPRRVEVRGEVFMPQAGFEQLNEEARRKGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGLLDGGELPRSHI
QCLMQFKAWGLPVSERVKLCTGSDQVIAFYRQIEQDRAGLGFDIDGVVIKVDDLVLQEQLGFVARAPRWATAFKFPAQEQ
ITQVREVEFQVGRTGAITPVARLEPVQVAGVIVSNATLHNADEIERLGLRIGDTVIVRRAGDVIPQVVGVVMEQRPDDTK
EITFPSQCPVCGSDIERVEGEAVARCTGGLFCAAQRKEALKHFVSRRALDVDGMGDKIIEQLVEKQYVENPADLFQLTAG
KLTGLDRMGPKSAQNLIAALEKAKQTTFARFLYALGIREVGEATAANLAAHFRTLDNLRAADIETLKSVPDVGEVVAKHV
MNFLSEEHNQKVIEELEKVVSWPEPQQIVVEEIDSPFAGKTVVLTGSLTILSRDEAKDRLTALGAKVSGSVSKKTHLVIA
GEAAGSKLAKAQELGIKVIDEAEMIRLLGE

Sequences:

>Translated_670_residues
MESIIQQINQLRTSLRHHEHQYHVLDAPEIPDAEYDRMMQQLRDLEAQHPELVTNDSPTQRVGAAPLDAFEQVEHEVPML
SLDNVFDEESYLAFDKRVHDRLKTAEPLTFCCELKLDGLAVSLLYENGELVRAATRGDGTTGENITANVRTIRAIPLRLH
GDNVPRRVEVRGEVFMPQAGFEQLNEEARRKGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGLLDGGELPRSHI
QCLMQFKAWGLPVSERVKLCTGSDQVIAFYRQIEQDRAGLGFDIDGVVIKVDDLVLQEQLGFVARAPRWATAFKFPAQEQ
ITQVREVEFQVGRTGAITPVARLEPVQVAGVIVSNATLHNADEIERLGLRIGDTVIVRRAGDVIPQVVGVVMEQRPDDTK
EITFPSQCPVCGSDIERVEGEAVARCTGGLFCAAQRKEALKHFVSRRALDVDGMGDKIIEQLVEKQYVENPADLFQLTAG
KLTGLDRMGPKSAQNLIAALEKAKQTTFARFLYALGIREVGEATAANLAAHFRTLDNLRAADIETLKSVPDVGEVVAKHV
MNFLSEEHNQKVIEELEKVVSWPEPQQIVVEEIDSPFAGKTVVLTGSLTILSRDEAKDRLTALGAKVSGSVSKKTHLVIA
GEAAGSKLAKAQELGIKVIDEAEMIRLLGE
>Mature_670_residues
MESIIQQINQLRTSLRHHEHQYHVLDAPEIPDAEYDRMMQQLRDLEAQHPELVTNDSPTQRVGAAPLDAFEQVEHEVPML
SLDNVFDEESYLAFDKRVHDRLKTAEPLTFCCELKLDGLAVSLLYENGELVRAATRGDGTTGENITANVRTIRAIPLRLH
GDNVPRRVEVRGEVFMPQAGFEQLNEEARRKGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGLLDGGELPRSHI
QCLMQFKAWGLPVSERVKLCTGSDQVIAFYRQIEQDRAGLGFDIDGVVIKVDDLVLQEQLGFVARAPRWATAFKFPAQEQ
ITQVREVEFQVGRTGAITPVARLEPVQVAGVIVSNATLHNADEIERLGLRIGDTVIVRRAGDVIPQVVGVVMEQRPDDTK
EITFPSQCPVCGSDIERVEGEAVARCTGGLFCAAQRKEALKHFVSRRALDVDGMGDKIIEQLVEKQYVENPADLFQLTAG
KLTGLDRMGPKSAQNLIAALEKAKQTTFARFLYALGIREVGEATAANLAAHFRTLDNLRAADIETLKSVPDVGEVVAKHV
MNFLSEEHNQKVIEELEKVVSWPEPQQIVVEEIDSPFAGKTVVLTGSLTILSRDEAKDRLTALGAKVSGSVSKKTHLVIA
GEAAGSKLAKAQELGIKVIDEAEMIRLLGE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=670, Percent_Identity=78.5074626865672, Blast_Score=1095, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=587, Percent_Identity=23.1686541737649, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 73954; Mature: 73954

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESIIQQINQLRTSLRHHEHQYHVLDAPEIPDAEYDRMMQQLRDLEAQHPELVTNDSPTQ
CHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCH
RVGAAPLDAFEQVEHEVPMLSLDNVFDEESYLAFDKRVHDRLKTAEPLTFCCELKLDGLA
HCCCCCHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCEE
VSLLYENGELVRAATRGDGTTGENITANVRTIRAIPLRLHGDNVPRRVEVRGEVFMPQAG
EEEEECCCCEEEEEECCCCCCCCCEEECEEEEEEEEEEEECCCCCCEEEECCCEECCCCC
FEQLNEEARRKGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGLLDGGELPRSHI
HHHHHHHHHHCCCCEEECCCCCHHCCHHHCCCCCCCCCCCEEEEEEEEECCCCCCCHHHH
QCLMQFKAWGLPVSERVKLCTGSDQVIAFYRQIEQDRAGLGFDIDGVVIKVDDLVLQEQL
HHHHHHHHCCCCHHHHHEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHH
GFVARAPRWATAFKFPAQEQITQVREVEFQVGRTGAITPVARLEPVQVAGVIVSNATLHN
HHHHCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCEEEEEEEECCCCCC
ADEIERLGLRIGDTVIVRRAGDVIPQVVGVVMEQRPDDTKEITFPSQCPVCGSDIERVEG
HHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCHHHHHCC
EAVARCTGGLFCAAQRKEALKHFVSRRALDVDGMGDKIIEQLVEKQYVENPADLFQLTAG
HHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
KLTGLDRMGPKSAQNLIAALEKAKQTTFARFLYALGIREVGEATAANLAAHFRTLDNLRA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ADIETLKSVPDVGEVVAKHVMNFLSEEHNQKVIEELEKVVSWPEPQQIVVEEIDSPFAGK
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCC
TVVLTGSLTILSRDEAKDRLTALGAKVSGSVSKKTHLVIAGEAAGSKLAKAQELGIKVID
EEEEEEEEEEEECHHHHHHHHHHCCHHCCCCCCCEEEEEEECCCCHHHHHHHHCCCEEEC
EAEMIRLLGE
HHHHHHHHCC
>Mature Secondary Structure
MESIIQQINQLRTSLRHHEHQYHVLDAPEIPDAEYDRMMQQLRDLEAQHPELVTNDSPTQ
CHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCH
RVGAAPLDAFEQVEHEVPMLSLDNVFDEESYLAFDKRVHDRLKTAEPLTFCCELKLDGLA
HCCCCCHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCEE
VSLLYENGELVRAATRGDGTTGENITANVRTIRAIPLRLHGDNVPRRVEVRGEVFMPQAG
EEEEECCCCEEEEEECCCCCCCCCEEECEEEEEEEEEEEECCCCCCEEEECCCEECCCCC
FEQLNEEARRKGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGLLDGGELPRSHI
HHHHHHHHHHCCCCEEECCCCCHHCCHHHCCCCCCCCCCCEEEEEEEEECCCCCCCHHHH
QCLMQFKAWGLPVSERVKLCTGSDQVIAFYRQIEQDRAGLGFDIDGVVIKVDDLVLQEQL
HHHHHHHHCCCCHHHHHEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHH
GFVARAPRWATAFKFPAQEQITQVREVEFQVGRTGAITPVARLEPVQVAGVIVSNATLHN
HHHHCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCEEEEEEEECCCCCC
ADEIERLGLRIGDTVIVRRAGDVIPQVVGVVMEQRPDDTKEITFPSQCPVCGSDIERVEG
HHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCHHHHHCC
EAVARCTGGLFCAAQRKEALKHFVSRRALDVDGMGDKIIEQLVEKQYVENPADLFQLTAG
HHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
KLTGLDRMGPKSAQNLIAALEKAKQTTFARFLYALGIREVGEATAANLAAHFRTLDNLRA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ADIETLKSVPDVGEVVAKHVMNFLSEEHNQKVIEELEKVVSWPEPQQIVVEEIDSPFAGK
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCC
TVVLTGSLTILSRDEAKDRLTALGAKVSGSVSKKTHLVIAGEAAGSKLAKAQELGIKVID
EEEEEEEEEEEECHHHHHHHHHHCCHHCCCCCCCEEEEEEECCCCHHHHHHHHCCCEEEC
EAEMIRLLGE
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA