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The map label for this gene is 170022738

Identifier: 170022738

GI number: 170022738

Start: 540921

End: 541394

Strand: Direct

Name: 170022738

Synonym: YPK_0487

Alternate gene names: NA

Gene position: 540921-541394 (Clockwise)

Preceding gene: 170022737

Following gene: 170022739

Centisome position: 11.53

GC content: 52.32

Gene sequence:

>474_bases
ATGAATAAACGACTGATGGCCATACTGGGTGCTGTGCTGCTATTGATAGCAGGTGCATGGCAAGGGATTGATGGCGTAAC
GGTGCGTGATATTGGTTCCTCGGCAGAGCTTGAGCGCCCGGCGCTTTCGGCCTCTGCCGCTACTGAATCGTTAACGCAAC
ACCAGAAAGTGGTGAAATACTTACAAACGCACCATCGGCTACCGGATTTTTACCTGACGAAGAAACAGGCCCGTGAGCAA
GGCTGGGATGCCCAAAAGGGGAACCTCTGTGACGTGTTACCGGGCAAAGCCATTGGCGGTGATCGTTTCTCCAACCGTGA
GCGTCAGTTGCCTGACGCCAAAGGCCGTCATTGGCGAGAAGCTGATGTGAATTACCGCTGTGGGCGTCGTGGTAGTGATC
GCTTGCTGTACTCCAATGATGGCCTGATTTATCTGACCCAAGATCACTATAAGCATTTCATTCGGATGGAGTAA

Upstream 100 bases:

>100_bases
ATGGCATTAATTGTAGGAATGATATTAATTGTAGGAACGATATTAAGGGCAACAATGAGTAGTACCGCCGTGGTAGGGAG
CCTGGGGATTAAGGGGATCC

Downstream 100 bases:

>100_bases
GAAGATGGTAAAGGTGGTATTCGATTTTAACCATATACCGGATCTTCCTGCATTTTACCGCGCATTTTCTCAGAATTTTG
CGCTGAGCGAAGATTTTGGT

Product: guanine-specific ribonuclease N1 and T1

Products: NA

Alternate protein names: Barnase; RNase Ba [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MNKRLMAILGAVLLLIAGAWQGIDGVTVRDIGSSAELERPALSASAATESLTQHQKVVKYLQTHHRLPDFYLTKKQAREQ
GWDAQKGNLCDVLPGKAIGGDRFSNRERQLPDAKGRHWREADVNYRCGRRGSDRLLYSNDGLIYLTQDHYKHFIRME

Sequences:

>Translated_157_residues
MNKRLMAILGAVLLLIAGAWQGIDGVTVRDIGSSAELERPALSASAATESLTQHQKVVKYLQTHHRLPDFYLTKKQAREQ
GWDAQKGNLCDVLPGKAIGGDRFSNRERQLPDAKGRHWREADVNYRCGRRGSDRLLYSNDGLIYLTQDHYKHFIRME
>Mature_157_residues
MNKRLMAILGAVLLLIAGAWQGIDGVTVRDIGSSAELERPALSASAATESLTQHQKVVKYLQTHHRLPDFYLTKKQAREQ
GWDAQKGNLCDVLPGKAIGGDRFSNRERQLPDAKGRHWREADVNYRCGRRGSDRLLYSNDGLIYLTQDHYKHFIRME

Specific function: Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonuclease N1/T1 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001887
- InterPro:   IPR000026
- InterPro:   IPR016191 [H]

Pfam domain/function: PF00545 Ribonuclease [H]

EC number: NA

Molecular weight: Translated: 17819; Mature: 17819

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRLMAILGAVLLLIAGAWQGIDGVTVRDIGSSAELERPALSASAATESLTQHQKVVKY
CCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LQTHHRLPDFYLTKKQAREQGWDAQKGNLCDVLPGKAIGGDRFSNRERQLPDAKGRHWRE
HHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCC
ADVNYRCGRRGSDRLLYSNDGLIYLTQDHYKHFIRME
CCCCCCCCCCCCCCEEEECCCEEEEEHHHHHHHHCCC
>Mature Secondary Structure
MNKRLMAILGAVLLLIAGAWQGIDGVTVRDIGSSAELERPALSASAATESLTQHQKVVKY
CCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LQTHHRLPDFYLTKKQAREQGWDAQKGNLCDVLPGKAIGGDRFSNRERQLPDAKGRHWRE
HHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCC
ADVNYRCGRRGSDRLLYSNDGLIYLTQDHYKHFIRME
CCCCCCCCCCCCCCEEEECCCEEEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3007290; 3050134; 4553460; 1888730; 1569554; 9708913; 2023257; 11914482; 2696173 [H]