Click here to switch to the map view.

The map label for this gene is Hgd [H]

Identifier: 170022698

GI number: 170022698

Start: 487710

End: 488600

Strand: Direct

Name: Hgd [H]

Synonym: YPK_0447

Alternate gene names: 170022698

Gene position: 487710-488600 (Clockwise)

Preceding gene: 170022697

Following gene: 170022699

Centisome position: 10.4

GC content: 51.07

Gene sequence:

>891_bases
ATGAAACAACTACAACGTATTGGTTTTATTGGTTTGGGGAAGATGGGAACCCCTATGGTACAGCGCCTGGTAAAGGCGGG
TTTTGAGCTGTATCTCTGTGATGCAGATATCACCAAGGTGCAAATACTGACGGCAGAACTCAACGCTGAATCACTCACGG
TCGATAATGCGGCATCTTTAGATGCCTTGATCACCATGTTGCCTAACTCTGAGGCCGTTGAACAGGCATTGCTGGGAAGT
GACGGGATATCGGGATGGGTTGCACAGCTATCTCAAGCGGCAGTGGTGATCGACATGAGTTCCTCCGATCCCGAACGCTC
ACGTCGTCTGGCGATCTTGCTGGCTGTATGGGAACTGGATTATCTGGATGCCCCGGTCTCTGGCGGGGTAAAAAAAGCCC
AAAACGGGACATTGTCGATCCTGATTGGGGGCGAGGACCGGGTACTTAAATCTTGTTATACCGCTTTGGCTGCGATGGGG
GAACAGATCCTGTTTGTTGGCCCTGCGGGGAGTGGTCATGCGGCCAAGGCATTGAATAATTATGTCTCAGCGACGGGGCT
ATTGGCGACAATCGAGGCACTGCATGTTGCCCAACGTTTTGGGATCGAACCAGAGGTGATGACCGAGGTGCTCAATACCT
CCACTGGCCGGAGTAATACGTCAGAAAACAAAGTGCGCCAATTTATGCTGAACGGTTCGTATGCCTCTGGTTTTACGCTG
CAATTGATGAATAAAGACCTGCATATCGCACGTAATTTGGCACAACGGCTTAATTACCCAATGAGATTGGGCATGCACTG
TGTCGATGTTTGGGATGAAGTTTCGCGGCGTGCAACACCGATGGCAGACCATACCGAGATGTACCGGCTGTTGATCGACA
AGGAGCCATAG

Upstream 100 bases:

>100_bases
CAGGTTGATGTTGCCCCCCTCACTCAGTGGACGTGAAATGTATTTCAGCCGAACGGATTAAATGCGTGCTCTGGCGACCA
ATAACGATTAAGGGGGTCAG

Downstream 100 bases:

>100_bases
CGTCGTAAAGGAGGCCGCCTTACGTAGCGAATACCTCGGGTTACGCTCAGGGGAAGCGTCAGGGGGAAAAAATTTTGTCT
AACCATCCTGTCATACAATC

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKQLQRIGFIGLGKMGTPMVQRLVKAGFELYLCDADITKVQILTAELNAESLTVDNAASLDALITMLPNSEAVEQALLGS
DGISGWVAQLSQAAVVIDMSSSDPERSRRLAILLAVWELDYLDAPVSGGVKKAQNGTLSILIGGEDRVLKSCYTALAAMG
EQILFVGPAGSGHAAKALNNYVSATGLLATIEALHVAQRFGIEPEVMTEVLNTSTGRSNTSENKVRQFMLNGSYASGFTL
QLMNKDLHIARNLAQRLNYPMRLGMHCVDVWDEVSRRATPMADHTEMYRLLIDKEP

Sequences:

>Translated_296_residues
MKQLQRIGFIGLGKMGTPMVQRLVKAGFELYLCDADITKVQILTAELNAESLTVDNAASLDALITMLPNSEAVEQALLGS
DGISGWVAQLSQAAVVIDMSSSDPERSRRLAILLAVWELDYLDAPVSGGVKKAQNGTLSILIGGEDRVLKSCYTALAAMG
EQILFVGPAGSGHAAKALNNYVSATGLLATIEALHVAQRFGIEPEVMTEVLNTSTGRSNTSENKVRQFMLNGSYASGFTL
QLMNKDLHIARNLAQRLNYPMRLGMHCVDVWDEVSRRATPMADHTEMYRLLIDKEP
>Mature_296_residues
MKQLQRIGFIGLGKMGTPMVQRLVKAGFELYLCDADITKVQILTAELNAESLTVDNAASLDALITMLPNSEAVEQALLGS
DGISGWVAQLSQAAVVIDMSSSDPERSRRLAILLAVWELDYLDAPVSGGVKKAQNGTLSILIGGEDRVLKSCYTALAAMG
EQILFVGPAGSGHAAKALNNYVSATGLLATIEALHVAQRFGIEPEVMTEVLNTSTGRSNTSENKVRQFMLNGSYASGFTL
QLMNKDLHIARNLAQRLNYPMRLGMHCVDVWDEVSRRATPMADHTEMYRLLIDKEP

Specific function: Catalyzes the conversion of 2-formylglutarate to (S)-2- hydroxymethylglutarate. Has very low activity with (S)-3- hydroxyisobutyrate [H]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=297, Percent_Identity=32.3232323232323, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI40556376, Length=289, Percent_Identity=24.9134948096886, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI145693186, Length=283, Percent_Identity=31.4487632508834, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1786719, Length=287, Percent_Identity=30.6620209059233, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1790315, Length=272, Percent_Identity=31.25, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789092, Length=291, Percent_Identity=27.4914089347079, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17557316, Length=297, Percent_Identity=31.6498316498317, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24655230, Length=296, Percent_Identity=32.0945945945946, Blast_Score=141, Evalue=5e-34,
Organism=Drosophila melanogaster, GI19922568, Length=296, Percent_Identity=32.0945945945946, Blast_Score=141, Evalue=5e-34,
Organism=Drosophila melanogaster, GI28574115, Length=286, Percent_Identity=24.1258741258741, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.291 [H]

Molecular weight: Translated: 32112; Mature: 32112

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLQRIGFIGLGKMGTPMVQRLVKAGFELYLCDADITKVQILTAELNAESLTVDNAASL
CCCHHHCCCEECCCCCCHHHHHHHHCCCEEEEECCCCCEEEEEEEECCCCEEECCCCHHH
DALITMLPNSEAVEQALLGSDGISGWVAQLSQAAVVIDMSSSDPERSRRLAILLAVWELD
HHHHHCCCCHHHHHHHHHCCCCCHHHHHHHCCCEEEEEECCCCCHHHHHEEEEEEEHHHH
YLDAPVSGGVKKAQNGTLSILIGGEDRVLKSCYTALAAMGEQILFVGPAGSGHAAKALNN
HCCCCCCCCCHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHH
YVSATGLLATIEALHVAQRFGIEPEVMTEVLNTSTGRSNTSENKVRQFMLNGSYASGFTL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEE
QLMNKDLHIARNLAQRLNYPMRLGMHCVDVWDEVSRRATPMADHTEMYRLLIDKEP
EEECCHHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKQLQRIGFIGLGKMGTPMVQRLVKAGFELYLCDADITKVQILTAELNAESLTVDNAASL
CCCHHHCCCEECCCCCCHHHHHHHHCCCEEEEECCCCCEEEEEEEECCCCEEECCCCHHH
DALITMLPNSEAVEQALLGSDGISGWVAQLSQAAVVIDMSSSDPERSRRLAILLAVWELD
HHHHHCCCCHHHHHHHHHCCCCCHHHHHHHCCCEEEEEECCCCCHHHHHEEEEEEEHHHH
YLDAPVSGGVKKAQNGTLSILIGGEDRVLKSCYTALAAMGEQILFVGPAGSGHAAKALNN
HCCCCCCCCCHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHH
YVSATGLLATIEALHVAQRFGIEPEVMTEVLNTSTGRSNTSENKVRQFMLNGSYASGFTL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEE
QLMNKDLHIARNLAQRLNYPMRLGMHCVDVWDEVSRRATPMADHTEMYRLLIDKEP
EEECCHHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA