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The map label for this gene is aroE

Identifier: 170022582

GI number: 170022582

Start: 342009

End: 342830

Strand: Direct

Name: aroE

Synonym: YPK_0321

Alternate gene names: 170022582

Gene position: 342009-342830 (Clockwise)

Preceding gene: 170022581

Following gene: 170022584

Centisome position: 7.29

GC content: 45.5

Gene sequence:

>822_bases
ATGGATCAGAAGTTTGCAGTATTTGGTAATCCTATTAGTCATAGTAAATCGCCGAGGATCCATACGCTTTTTTCCGAGCA
AACAGGCATTGAACATCGATATGGTAAGGTATTAGCTCCTTCTGAAGCCTTTGAGAACACATTAGTATCTTTTTTTGCTG
ATGGTGCCCAGGGAGCGAACATTACCACACCGTTTAAAGAACGCGCATATGACCAATGTGATGAATTAACCGACCGTGCA
TCTCTGGCAGGGGCTGTTAATACGATTAAGCGTTTGGAAGATGGGCGCTTGTTGGGTGACAACACAGATGGTATTGGTTT
GCTCAGTGATCTTGAACGACAAAACTTGATCCGAACAACTGATCATATTTTGTTGGTTGGGGCTGGTGGCGCTGCCCGAG
GAGTGATCCTTCCTCTACTTTCTTATGGGTGTACTGTGGTTGTTACCAACCGGACACATACCCGGGCGCAGCAACTGGCC
AAAGTATTTAACCATATTGGTGATATTGATGTTTGTGAAATGTCGGAGTTGGCTGGACAACGATTCGATTTGGTTATAAA
TGCGACGGCATCAGGCCTTCATGGAGAGGTACCTAACTTACCAGCAGCTATACTTACTTCTCAAACACGTTGTTACGATA
TGTTCTATCAGGCGGGAACCACACCATTCTTGGCTTGGGCACAACGATTGGGTTTAGCAGATTATGCCGATGGTTTAGGA
ATGCTGGTAGGGCAAGCAGCACATGCTTTCAAACTTTGGCACGGTGTGATGCCTGAAATTACGCCAGTATTAGCCCAGCT
ACGCAGTGAGTTAGGTAAATAG

Upstream 100 bases:

>100_bases
CATTACCGGTGTTATCCGGTCACGTTGGTGGCCGTTTGAATCCATCTGAAATCAGAGATGCCTTAACGGGTAAGCGGTTT
CGCCAAGGGTAGGATATTGT

Downstream 100 bases:

>100_bases
AAGCACACACCAATGAATGCAGGGAGGTTATATTTCGCCCTGCATATCATTCGGTTATTTAGACTCTGCCAGATAGTCAT
CTTTCCAGCGAACGTAGTTG

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MDQKFAVFGNPISHSKSPRIHTLFSEQTGIEHRYGKVLAPSEAFENTLVSFFADGAQGANITTPFKERAYDQCDELTDRA
SLAGAVNTIKRLEDGRLLGDNTDGIGLLSDLERQNLIRTTDHILLVGAGGAARGVILPLLSYGCTVVVTNRTHTRAQQLA
KVFNHIGDIDVCEMSELAGQRFDLVINATASGLHGEVPNLPAAILTSQTRCYDMFYQAGTTPFLAWAQRLGLADYADGLG
MLVGQAAHAFKLWHGVMPEITPVLAQLRSELGK

Sequences:

>Translated_273_residues
MDQKFAVFGNPISHSKSPRIHTLFSEQTGIEHRYGKVLAPSEAFENTLVSFFADGAQGANITTPFKERAYDQCDELTDRA
SLAGAVNTIKRLEDGRLLGDNTDGIGLLSDLERQNLIRTTDHILLVGAGGAARGVILPLLSYGCTVVVTNRTHTRAQQLA
KVFNHIGDIDVCEMSELAGQRFDLVINATASGLHGEVPNLPAAILTSQTRCYDMFYQAGTTPFLAWAQRLGLADYADGLG
MLVGQAAHAFKLWHGVMPEITPVLAQLRSELGK
>Mature_273_residues
MDQKFAVFGNPISHSKSPRIHTLFSEQTGIEHRYGKVLAPSEAFENTLVSFFADGAQGANITTPFKERAYDQCDELTDRA
SLAGAVNTIKRLEDGRLLGDNTDGIGLLSDLERQNLIRTTDHILLVGAGGAARGVILPLLSYGCTVVVTNRTHTRAQQLA
KVFNHIGDIDVCEMSELAGQRFDLVINATASGLHGEVPNLPAAILTSQTRCYDMFYQAGTTPFLAWAQRLGLADYADGLG
MLVGQAAHAFKLWHGVMPEITPVLAQLRSELGK

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1789675, Length=269, Percent_Identity=61.7100371747212, Blast_Score=356, Evalue=1e-100,
Organism=Escherichia coli, GI1787983, Length=264, Percent_Identity=29.9242424242424, Blast_Score=81, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6320332, Length=276, Percent_Identity=27.8985507246377, Blast_Score=90, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_YERP3 (A7FNJ7)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001402825.1
- ProteinModelPortal:   A7FNJ7
- SMR:   A7FNJ7
- STRING:   A7FNJ7
- GeneID:   5387448
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_3877
- eggNOG:   COG0169
- HOGENOM:   HBG553408
- OMA:   FAAQTGI
- ProtClustDB:   PRK00258
- BioCyc:   YPSE349747:YPSIP31758_3877-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 29595; Mature: 29595

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: ACT_SITE 66-66

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQKFAVFGNPISHSKSPRIHTLFSEQTGIEHRYGKVLAPSEAFENTLVSFFADGAQGAN
CCCCEEEECCCCCCCCCCCEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
ITTPFKERAYDQCDELTDRASLAGAVNTIKRLEDGRLLGDNTDGIGLLSDLERQNLIRTT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHCC
DHILLVGAGGAARGVILPLLSYGCTVVVTNRTHTRAQQLAKVFNHIGDIDVCEMSELAGQ
CCEEEEECCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHCCC
RFDLVINATASGLHGEVPNLPAAILTSQTRCYDMFYQAGTTPFLAWAQRLGLADYADGLG
EEEEEEECCCCCCCCCCCCCCHHHHHCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHH
MLVGQAAHAFKLWHGVMPEITPVLAQLRSELGK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDQKFAVFGNPISHSKSPRIHTLFSEQTGIEHRYGKVLAPSEAFENTLVSFFADGAQGAN
CCCCEEEECCCCCCCCCCCEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
ITTPFKERAYDQCDELTDRASLAGAVNTIKRLEDGRLLGDNTDGIGLLSDLERQNLIRTT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHCC
DHILLVGAGGAARGVILPLLSYGCTVVVTNRTHTRAQQLAKVFNHIGDIDVCEMSELAGQ
CCEEEEECCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHCCC
RFDLVINATASGLHGEVPNLPAAILTSQTRCYDMFYQAGTTPFLAWAQRLGLADYADGLG
EEEEEEECCCCCCCCCCCCCCHHHHHCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHH
MLVGQAAHAFKLWHGVMPEITPVLAQLRSELGK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA