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The map label for this gene is tusD

Identifier: 170022533

GI number: 170022533

Start: 311732

End: 312127

Strand: Direct

Name: tusD

Synonym: YPK_0272

Alternate gene names: 170022533

Gene position: 311732-312127 (Clockwise)

Preceding gene: 170022532

Following gene: 170022534

Centisome position: 6.65

GC content: 52.53

Gene sequence:

>396_bases
ATGTCCGCGTTAAAATATTGCCTATTGGTCACAGGGCCTGCTTATGGTACACAGCAGGCCAGTAGCGCTTATCAGTTCGC
TCAGGCGGTTGTTGGGGCAGGGCATCACTTGGTCAGCATCTTTTTCTATCGCGAAGGCGTACTGAATGCCAACCAACTCA
CGGCCCCAGCGAGTGATGAATTTGATCTGGTACGAGCATGGCAACAATTGGCGGCTGAACAGGCGGTTACGCTGAATGTG
TGTGTCGCGGCAGCGCTACGCCGTGGTATTACCGATCAACATGAAGCCGAGCAACTCAATTTGGCCGCTGCGAATCTGCA
ACCCGGTTTTACATTGAGTGGTCTAGGGGCGCTGGCAGAAGCTACGTTGACTTGTGATCGCATGGTGCAATTTTAA

Upstream 100 bases:

>100_bases
CCATAAACCAAGTTGCAGAACGCCTCAATATCTCAAAGCACACGGTATATCTTTACATTCGTCAGTTCAAAAGTGGCGAT
CTCGTCGGGCACGAACGTTA

Downstream 100 bases:

>100_bases
TGGCAAGAAAACGCATCGCTTTTATTTTTACTCAAGGCCCCCATGGCAGCTCAGCTGGACGTGAAGGGTTGGATGCTTTG
CTGGCCACCTCTGCTTTGAG

Product: sulfur transfer complex subunit TusD

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein D

Number of amino acids: Translated: 131; Mature: 130

Protein sequence:

>131_residues
MSALKYCLLVTGPAYGTQQASSAYQFAQAVVGAGHHLVSIFFYREGVLNANQLTAPASDEFDLVRAWQQLAAEQAVTLNV
CVAAALRRGITDQHEAEQLNLAAANLQPGFTLSGLGALAEATLTCDRMVQF

Sequences:

>Translated_131_residues
MSALKYCLLVTGPAYGTQQASSAYQFAQAVVGAGHHLVSIFFYREGVLNANQLTAPASDEFDLVRAWQQLAAEQAVTLNV
CVAAALRRGITDQHEAEQLNLAAANLQPGFTLSGLGALAEATLTCDRMVQF
>Mature_130_residues
SALKYCLLVTGPAYGTQQASSAYQFAQAVVGAGHHLVSIFFYREGVLNANQLTAPASDEFDLVRAWQQLAAEQAVTLNVC
VAAALRRGITDQHEAEQLNLAAANLQPGFTLSGLGALAEATLTCDRMVQF

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Accepts sulfur from tusA and transfers it in turn to tusE

COG id: COG1553

COG function: function code P; Uncharacterized conserved protein involved in intracellular sulfur reduction

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrE/tusD family

Homologues:

Organism=Escherichia coli, GI1789743, Length=128, Percent_Identity=73.4375, Blast_Score=202, Evalue=7e-54,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): TUSD_YERP3 (A7FNP4)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001402872.1
- ProteinModelPortal:   A7FNP4
- SMR:   A7FNP4
- STRING:   A7FNP4
- GeneID:   5387724
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_3924
- eggNOG:   COG1553
- HOGENOM:   HBG538485
- OMA:   QDGVYNS
- ProtClustDB:   PRK00207
- BioCyc:   YPSE349747:YPSIP31758_3924-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00390
- InterPro:   IPR003787
- InterPro:   IPR017463
- TIGRFAMs:   TIGR03012

Pfam domain/function: PF02635 DrsE

EC number: NA

Molecular weight: Translated: 13959; Mature: 13828

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: ACT_SITE 81-81

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALKYCLLVTGPAYGTQQASSAYQFAQAVVGAGHHLVSIFFYREGVLNANQLTAPASDE
CCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCC
FDLVRAWQQLAAEQAVTLNVCVAAALRRGITDQHEAEQLNLAAANLQPGFTLSGLGALAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHH
ATLTCDRMVQF
HHHHHHHHHCC
>Mature Secondary Structure 
SALKYCLLVTGPAYGTQQASSAYQFAQAVVGAGHHLVSIFFYREGVLNANQLTAPASDE
CCHHEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCC
FDLVRAWQQLAAEQAVTLNVCVAAALRRGITDQHEAEQLNLAAANLQPGFTLSGLGALAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHH
ATLTCDRMVQF
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA