Click here to switch to the map view.

The map label for this gene is ilvA [H]

Identifier: 169834141

GI number: 169834141

Start: 502646

End: 503896

Strand: Direct

Name: ilvA [H]

Synonym: SPH_0556

Alternate gene names: 169834141

Gene position: 502646-503896 (Clockwise)

Preceding gene: 169833391

Following gene: 169834350

Centisome position: 22.38

GC content: 41.01

Gene sequence:

>1251_bases
ATGTTAAGTTCAAAAGATATAATCAAGGCTCACAAGGTCTTGAACGGTGTGGTTGTGAATACTCCACTGGATTACGATCA
TTATTTATCGGAGAAGTATGGTGCTAAGATTTATTTGAAAAAAGAAAATGCCCAGCGTGTTCGCTCCTTTAAAATTCGTG
GTGCCTATTATGCCATTTCCCAGCTCAGCAAGGAAGAACGTGAACGTGGGGTAGTTTGCGCTTCTGCGGGAAATCATGCG
CAGGGAGTAGCCTATACTTGTAATGAAATGAAAATTCCTGCTACTATCTTTATGCCTATTACTACGCCACAACAAAAGAT
TGGTCAGGTTCGCTTTTTTGGTGGGGATTTTGTAACTATTAAACTAGTTGGAGATACCTTTGATGCCTCAGCCAAAGCAG
CTCAAGAATTTACAGTCTCTGAAAATCGTACCTTTATTGATCCTTTTGATGATGCTCATGTTCAAGCAGGTCAAGGAACA
GTTGCTTATGAGATTTTAGAAGAAGCTCGAAAAGAATCGATTGATTTTGATGCTGTCTTGGTTCCTGTTGGTGGTGGCGG
TCTCATTGCCGGGGTTTCTACCTATATCAAGGAAACAAGTCCAGAGATTGAGGTTATCGGAGTAGAGGCGAATGGAGCGC
GTTCCATGAAAGCTGCCTTTGAGGCTGGAGGTCCAGTAAAACTCAAGGAAATTGATAAATTTGCTGATGGGATTGCTGTG
CAAAAGGTAGGTCAGTTGACCTATGAAGCAACTCGTCAACATATTCAAACTTTGGTAGGTGTCGATGAGGGATTGATTTC
TGAAACCTTGATTGACCTTTACTCTAAGCAAGGGATAGTCGCAGAACCTGCTGGAGCGGCTAGTATCGCCTCTTTAGAGG
TTTTAGCTGAATATATTAAGGGGAAAACCATTTGTTGTATCATTTCTGGAGGAAATAATGATATCAACCGTATGCCAGAA
ATGGAAGAGCGTGCCTTGATTTATGATGGTATCAAACATTATTTTGTGGTCAATTTCCCACAACGTCCAGGAGCTTTGCG
TGAGTTTGTAAATGATATCCTGGGGCCAAATGATGATATCACACGTTTTGAGTATATCAAACGAGCTAGCAAGGGAACAG
GCCCAGTATTAATTGGGATCGCTTTAGCAGATAAGCATGATTATGCAGGTTTGATCCGTAGAATGGAAGGTTTTGATCCA
GCTTATATTAACTTAAATGGTAATGAAACGCTTTATAATATGCTTGTCTGA

Upstream 100 bases:

>100_bases
ATGGCTCATAAATACCATGAAAGTGTTACTGAAGTTTTCGGAGATGAATAACTAAAAAACAGTCATTAGTGACTGTTTTT
TATAGAAAAAGAGGTTTTAT

Downstream 100 bases:

>100_bases
GGACTAATAAAAAAATATCATATTATTTGCACAACTGATGTATATGTGCTATAATGAAGATGAAGAAAGGGGACGATTCC
TATGGTTTTAGGAAACTTAT

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENAQRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHA
QGVAYTCNEMKIPATIFMPITTPQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKAAFEAGGPVKLKEIDKFADGIAV
QKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSKQGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPE
MEERALIYDGIKHYFVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLIRRMEGFDP
AYINLNGNETLYNMLV

Sequences:

>Translated_416_residues
MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENAQRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHA
QGVAYTCNEMKIPATIFMPITTPQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKAAFEAGGPVKLKEIDKFADGIAV
QKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSKQGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPE
MEERALIYDGIKHYFVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLIRRMEGFDP
AYINLNGNETLYNMLV
>Mature_416_residues
MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENAQRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHA
QGVAYTCNEMKIPATIFMPITTPQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKAAFEAGGPVKLKEIDKFADGIAV
QKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSKQGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPE
MEERALIYDGIKHYFVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLIRRMEGFDP
AYINLNGNETLYNMLV

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=324, Percent_Identity=28.7037037037037, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI33469958, Length=282, Percent_Identity=32.2695035460993, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI19923959, Length=309, Percent_Identity=25.8899676375405, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1790207, Length=365, Percent_Identity=35.8904109589041, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1789505, Length=318, Percent_Identity=39.622641509434, Blast_Score=190, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI71991565, Length=345, Percent_Identity=35.0724637681159, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17537387, Length=370, Percent_Identity=32.972972972973, Blast_Score=144, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17508781, Length=285, Percent_Identity=29.4736842105263, Blast_Score=118, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6320930, Length=355, Percent_Identity=35.4929577464789, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6322631, Length=312, Percent_Identity=32.0512820512821, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6319788, Length=282, Percent_Identity=24.468085106383, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6322024, Length=162, Percent_Identity=24.6913580246914, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI21355833, Length=350, Percent_Identity=34.8571428571429, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24645328, Length=253, Percent_Identity=33.201581027668, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR001721
- InterPro:   IPR011820 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 45463; Mature: 45463

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENAQRVRSFKIRGAYYAIS
CCCCHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCEEEEECCCHHHHHHEEECHHHHHHH
QLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITTPQQKIGQVRFFGGDFVTI
HHHHHHHHCCEEEECCCCCCCCEEEEECCEECCEEEEEECCCCHHHHCEEEEECCCEEEE
KLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGTVAYEILEEARKESIDFDAVL
EEECCCCCCCHHHHHHEEECCCCEEECCCCCCCEECCCCCHHHHHHHHHHHCCCCCCEEE
VPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKAAFEAGGPVKLKEIDKFADGIAV
EEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHCCCCEEHHHHHHHHCCHHH
QKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSKQGIVAEPAGAASIASLEVLAEYIK
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHC
GKTICCIISGGNNDINRMPEMEERALIYDGIKHYFVVNFPQRPGALREFVNDILGPNDDI
CCEEEEEEECCCCCHHCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCH
TRFEYIKRASKGTGPVLIGIALADKHDYAGLIRRMEGFDPAYINLNGNETLYNMLV
HHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCEEEEECCCCEEHHHCC
>Mature Secondary Structure
MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENAQRVRSFKIRGAYYAIS
CCCCHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCEEEEECCCHHHHHHEEECHHHHHHH
QLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITTPQQKIGQVRFFGGDFVTI
HHHHHHHHCCEEEECCCCCCCCEEEEECCEECCEEEEEECCCCHHHHCEEEEECCCEEEE
KLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGTVAYEILEEARKESIDFDAVL
EEECCCCCCCHHHHHHEEECCCCEEECCCCCCCEECCCCCHHHHHHHHHHHCCCCCCEEE
VPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKAAFEAGGPVKLKEIDKFADGIAV
EEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHCCCCEEHHHHHHHHCCHHH
QKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSKQGIVAEPAGAASIASLEVLAEYIK
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHC
GKTICCIISGGNNDINRMPEMEERALIYDGIKHYFVVNFPQRPGALREFVNDILGPNDDI
CCEEEEEEECCCCCHHCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCH
TRFEYIKRASKGTGPVLIGIALADKHDYAGLIRRMEGFDPAYINLNGNETLYNMLV
HHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCEEEEECCCCEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400210; 11337471 [H]