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The map label for this gene is rfbD [H]

Identifier: 169834012

GI number: 169834012

Start: 418998

End: 419849

Strand: Direct

Name: rfbD [H]

Synonym: SPH_0469

Alternate gene names: 169834012

Gene position: 418998-419849 (Clockwise)

Preceding gene: 169832836

Following gene: 169833292

Centisome position: 18.66

GC content: 41.31

Gene sequence:

>852_bases
ATGATTTTAATTACAGGGGCAAATGGCCAATTAGGAACGGAACTTCGCTATTTATTGGATGAACGTAATGAAGAATACGT
GGCAGTAGATGTGGCTGAGATGGACATTACCGATGCAGAAATGGTTGAGAAAGTTTTTGAAGAGGTGAAACCGACTTTAG
TCTACCACTGTGCAGCCTACACCGCTGTTGATGCAGCAGAGGATGAAGGAAGAGAGTTGGACTTCGCCATCAATGTGACG
GGGACAAAAAATGTCGCAAAAGCATCTGAAAAGCATGGTGCAACTCTAGTTTATATTTCTACGGACTATGTCTTTGATGG
TAAGAAACCAGTTGGACAAGAGTGGGAAGTTGATGACCGACCAGATCCACAGACAGAATATGGCCGTACTAAGCGTATGG
GGGAAGAGTTAGTTGAGAAGCATGTGTCTAATTTCTATATTATCCGTACTGCCTGGGTATTTGGAAATTATGGCAAAAAC
TTCGTTTTTACCATGCAAAATCTTGCGAAAACTCATAAGACTTTAACAGTTGTAAATGACCAGTACGGTCGTCCGACTTG
GACTCGTACCTTGGCTGAGTTCATGACCTACCTAGCTGAAAATCGTAAGGAATTTGGTTATTATCATTTGTCAAATGATG
CGACAGAAGACACAACATGGTATGATTTTGCAGTTGAAATTTTGAAAGATACAGATGTCGAAGTCAAGCCAGTAGATTCC
AGTCAATTTCCAGCCAAAGCTAAACGTCCGCTAAACTCAACGATGAGCCTGGCCAAAGCCAAAGCTACTGGATTTGTTAT
TCCAACTTGGCAAGATGCATTGCAAGAATTTTACAAACAAGAAGTGAGATAA

Upstream 100 bases:

>100_bases
ATTATGCTAAGACTCAGGAGATTATTACAGTATAAAAAGCAGGAAATAGCTGCTTTTTATTGCTATATTGGGAAGAGTTA
CATATTAGAAAGGTCTAGAG

Downstream 100 bases:

>100_bases
GTAGTAGAATGATTTTCTAGTCTAATAAAAGAGGCAGATAATGAACTCCAAAGGAGCTTAAGATGTACGATTATCTTGTT
GTTGGTGCTGGTCTCTTTGG

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITDAEMVEKVFEEVKPTLVYHCAAYTAVDAAEDEGRELDFAINVT
GTKNVAKASEKHGATLVYISTDYVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDTTWYDFAVEILKDTDVEVKPVDS
SQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQEFYKQEVR

Sequences:

>Translated_283_residues
MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITDAEMVEKVFEEVKPTLVYHCAAYTAVDAAEDEGRELDFAINVT
GTKNVAKASEKHGATLVYISTDYVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDTTWYDFAVEILKDTDVEVKPVDS
SQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQEFYKQEVR
>Mature_283_residues
MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITDAEMVEKVFEEVKPTLVYHCAAYTAVDAAEDEGRELDFAINVT
GTKNVAKASEKHGATLVYISTDYVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDTTWYDFAVEILKDTDVEVKPVDS
SQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQEFYKQEVR

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=282, Percent_Identity=26.5957446808511, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI33519455, Length=282, Percent_Identity=26.5957446808511, Blast_Score=91, Evalue=8e-19,
Organism=Escherichia coli, GI1788352, Length=296, Percent_Identity=33.445945945946, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 32331; Mature: 32331

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITDAEMVEKVFEEVKPTLVYHCAAY
CEEEECCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHH
TAVDAAEDEGRELDFAINVTGTKNVAKASEKHGATLVYISTDYVFDGKKPVGQEWEVDDR
HHHCCCCCCCCEEEEEEEECCCCHHHHHHHHCCCEEEEEEECEEECCCCCCCCCCCCCCC
PDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKNFVFTMQNLAKTHKTLTVVND
CCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCEEEHHHHHHHHHEEEEECC
QYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDTTWYDFAVEILKDTDVEVKPVDS
CCCCCHHHHHHHHHHHHHHHCCHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCC
SQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQEFYKQEVR
CCCCCHHCCCCHHHHHHHHHHHCCEEEECHHHHHHHHHHHHCC
>Mature Secondary Structure
MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITDAEMVEKVFEEVKPTLVYHCAAY
CEEEECCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHH
TAVDAAEDEGRELDFAINVTGTKNVAKASEKHGATLVYISTDYVFDGKKPVGQEWEVDDR
HHHCCCCCCCCEEEEEEEECCCCHHHHHHHHCCCEEEEEEECEEECCCCCCCCCCCCCCC
PDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKNFVFTMQNLAKTHKTLTVVND
CCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCEEEHHHHHHHHHEEEEECC
QYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDTTWYDFAVEILKDTDVEVKPVDS
CCCCCHHHHHHHHHHHHHHHCCHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCC
SQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQEFYKQEVR
CCCCCHHCCCCHHHHHHHHHHHCCEEEECHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]