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The map label for this gene is prfC [H]

Identifier: 169833615

GI number: 169833615

Start: 493410

End: 494954

Strand: Direct

Name: prfC [H]

Synonym: SPH_0545

Alternate gene names: 169833615

Gene position: 493410-494954 (Clockwise)

Preceding gene: 169833259

Following gene: 169834016

Centisome position: 21.97

GC content: 45.83

Gene sequence:

>1545_bases
ATGAATATTCAAGAAGAAATTAAGAAACGTCGTACCTTTGCCATTATTTCCCACCCGGACGCGGGGAAAACAACCATCAC
TGAGCAGTTACTCTACTTTGGGGGTGAGATTCGTGAGGCTGGTACGGTAAAAGGGAAGAAAACAGGGACTTTTGCTAAAT
CTGACTGGATGGATATCGAGAAGCAACGTGGGATTTCTGTTACTTCATCTGTTATGCAATTTGACTACGACGGCAAGCGC
GTGAATATCTTAGACACGCCAGGGCACGAGGACTTCTCAGAAGATACCTATCGTACCTTGATGGCGGTGGATGCTGCGGT
CATGGTCGTGGACTCTGCCAAGGGGATCGAGGCTCAAACCAAGAAATTGTTTGAGGTTGTGAAACATCGTGGCATTCCAG
TCTTTACCTTTATGAACAAGCTGGACCGTGACGGTCGTGAGCCTTTGGATCTGTTGCAAGAATTGGAAGAAATCTTGGGC
ATTGCTAGCTACCCTATGAACTGGCCTATCGGGATGGGGAAAGCCTTTGAGGGCTTGTATGACCTCTATAACCAACGTTT
AGAGCTTTACAAAGGGGATGAGCGTTTTGCCAGTCTAGAAGACGGGGACAAACTCTTTGGTAGCAATCCTTTCTACGAGC
AAGTCAAGGATGACATTGAGCTTTTAAATGAAGCCGGGAATGAGTTTTCAGAGGAAGCTATTTTGGCTGGAGAATTGACG
CCTGTCTTTTTCGGTTCAGCCCTGACAAACTTTGGTGTGCAGACCTTCCTTGAAACCTTCCTCAAGTTTGCTCCAGAACC
ACATGGTCACAAGAAAACAGACGGTGAAATTGTGGATCCTTATGACAAGGATTTCTCAGGCTTTGTCTTTAAAATCCAAG
CCAACATGGATCCTCGTCACCGTGACCGTATTGCCTTTGTCCGTATCGTATCAGGCGAATTTGAGCGCGGCATGAGTGTC
AATCTCCCTCGTACTGGTAAGGGGGCTAAACTATCTAATGTTACCCAGTTTATGGCGGAGAGTCGTGAGAATGTGACCAA
TGCCGTGGCAGGTGATATTATCGGGGTTTACGATACCGGTACTTATCAGGTTGGGGATACCTTGACGGTTGGAAAAAACA
AGTTTGAATTTGAACCACTGCCAACCTTTACTCCTGAAATTTTCATGAAAGTTTCTGCTAAGAATGTTATGAAGCAAAAA
TCCTTCCACAAGGGGATTGAGCAATTGGTGCAAGAAGGAGCCGTTCAGCTTTATAAGAATTACCAAACAAGTGAGTACAT
GCTAGGCGCTGTTGGTCAACTCCAGTTTGAAGTCTTTAAACACCGTATGGAAGGCGAATACAATGCGGAAGTGGTCATGA
GCCCAATGGGTAAAAAGACCGTTCGTTGGATCAAGCCTGAGGACTTGGATGAACGGATGTCGTCAAGTCGCAATATCTTA
GCCAAAGACCGTTTTGACCAACCAGTCTTTCTCTTTGAAAATGACTTTGCCCTCCGCTGGTTTGCGGACAAGTATCCAGA
CGTAGAGTTAGAGGAGAAGATGTGA

Upstream 100 bases:

>100_bases
TCCGATATAATGGAGTGTTGAAAGGAATGGTAAAATCCAATGTAAAAATCATTCTCAGCTATTGAAACAAGAAAAATAGA
GAATCAAAGAAAGAGAACTT

Downstream 100 bases:

>100_bases
CTCAGTACCAACAATTGGAACTAAAGTTCCAAATTGTTGGACGCTAGTCGCTATTTGGCGAACTAGCTAACTGCCTTACT
AACTACCTTACTAACTACGT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVKGKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKR
VNILDTPGHEDFSEDTYRTLMAVDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDDIELLNEAGNEFSEEAILAGELT
PVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGEIVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSV
NLPRTGKGAKLSNVTQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVSAKNVMKQK
SFHKGIEQLVQEGAVQLYKNYQTSEYMLGAVGQLQFEVFKHRMEGEYNAEVVMSPMGKKTVRWIKPEDLDERMSSSRNIL
AKDRFDQPVFLFENDFALRWFADKYPDVELEEKM

Sequences:

>Translated_514_residues
MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVKGKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKR
VNILDTPGHEDFSEDTYRTLMAVDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDDIELLNEAGNEFSEEAILAGELT
PVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGEIVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSV
NLPRTGKGAKLSNVTQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVSAKNVMKQK
SFHKGIEQLVQEGAVQLYKNYQTSEYMLGAVGQLQFEVFKHRMEGEYNAEVVMSPMGKKTVRWIKPEDLDERMSSSRNIL
AKDRFDQPVFLFENDFALRWFADKYPDVELEEKM
>Mature_514_residues
MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVKGKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKR
VNILDTPGHEDFSEDTYRTLMAVDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDDIELLNEAGNEFSEEAILAGELT
PVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGEIVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSV
NLPRTGKGAKLSNVTQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVSAKNVMKQK
SFHKGIEQLVQEGAVQLYKNYQTSEYMLGAVGQLQFEVFKHRMEGEYNAEVVMSPMGKKTVRWIKPEDLDERMSSSRNIL
AKDRFDQPVFLFENDFALRWFADKYPDVELEEKM

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=482, Percent_Identity=30.0829875518672, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI19923640, Length=511, Percent_Identity=27.3972602739726, Blast_Score=172, Evalue=8e-43,
Organism=Homo sapiens, GI25306283, Length=399, Percent_Identity=30.0751879699248, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI25306287, Length=294, Percent_Identity=33.3333333333333, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI157426893, Length=157, Percent_Identity=32.484076433121, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI94966754, Length=147, Percent_Identity=27.2108843537415, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI4503483, Length=150, Percent_Identity=29.3333333333333, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1790835, Length=521, Percent_Identity=47.9846449136276, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI1789738, Length=487, Percent_Identity=27.3100616016427, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI1788922, Length=160, Percent_Identity=33.125, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI48994988, Length=179, Percent_Identity=31.8435754189944, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789737, Length=143, Percent_Identity=29.3706293706294, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1790412, Length=143, Percent_Identity=29.3706293706294, Blast_Score=63, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17533571, Length=496, Percent_Identity=28.4274193548387, Blast_Score=207, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17556745, Length=180, Percent_Identity=34.4444444444444, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17557151, Length=137, Percent_Identity=31.3868613138686, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17506493, Length=552, Percent_Identity=23.1884057971014, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=502, Percent_Identity=26.8924302788845, Blast_Score=191, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6322359, Length=515, Percent_Identity=26.0194174757282, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324707, Length=549, Percent_Identity=20.9471766848816, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320593, Length=549, Percent_Identity=20.9471766848816, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=29.7101449275362, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24582462, Length=486, Percent_Identity=28.3950617283951, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI221458488, Length=502, Percent_Identity=26.0956175298805, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585711, Length=547, Percent_Identity=21.7550274223035, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24585713, Length=547, Percent_Identity=21.7550274223035, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24585709, Length=547, Percent_Identity=21.7550274223035, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR002127
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 58489; Mature: 58489

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVKGKKTGTFAKSDWMDIE
CCHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
KQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMAVDAAVMVVDSAKGIEAQT
HHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHEEEECCCCCHHHH
KKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILGIASYPMNWPIGMGKAFEGLY
HHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
DLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDDIELLNEAGNEFSEEAILAGELT
HHHHHHHHHHCCCHHHCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCHHHHCCEEECCCC
PVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGEIVDPYDKDFSGFVFKIQANMDPRH
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCEEEEEECCCCCCC
RDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNVTQFMAESRENVTNAVAGDIIGVYDTG
CCCEEEEEEECCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECC
TYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVSAKNVMKQKSFHKGIEQLVQEGAVQLYKN
CEECCCEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YQTSEYMLGAVGQLQFEVFKHRMEGEYNAEVVMSPMGKKTVRWIKPEDLDERMSSSRNIL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEECCCCCCHHHHHHCCCCEE
AKDRFDQPVFLFENDFALRWFADKYPDVELEEKM
ECCCCCCCEEEEECCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVKGKKTGTFAKSDWMDIE
CCHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
KQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMAVDAAVMVVDSAKGIEAQT
HHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHEEEECCCCCHHHH
KKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILGIASYPMNWPIGMGKAFEGLY
HHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
DLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDDIELLNEAGNEFSEEAILAGELT
HHHHHHHHHHCCCHHHCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCHHHHCCEEECCCC
PVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGEIVDPYDKDFSGFVFKIQANMDPRH
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCEEEEEECCCCCCC
RDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNVTQFMAESRENVTNAVAGDIIGVYDTG
CCCEEEEEEECCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECC
TYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVSAKNVMKQKSFHKGIEQLVQEGAVQLYKN
CEECCCEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YQTSEYMLGAVGQLQFEVFKHRMEGEYNAEVVMSPMGKKTVRWIKPEDLDERMSSSRNIL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEECCCCCCHHHHHHCCCCEE
AKDRFDQPVFLFENDFALRWFADKYPDVELEEKM
ECCCCCCCEEEEECCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA