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The map label for this gene is pflB [H]

Identifier: 169833612

GI number: 169833612

Start: 513453

End: 515777

Strand: Direct

Name: pflB [H]

Synonym: SPH_0569

Alternate gene names: 169833612

Gene position: 513453-515777 (Clockwise)

Preceding gene: 169833255

Following gene: 169832403

Centisome position: 22.86

GC content: 41.94

Gene sequence:

>2325_bases
ATGGTTGTTAAGACAGTTGTTGAAGCACAAGATATTTTTGACAAAGCTTGGGAAGGCTTCAAAGGCGTAGATTGGAAAGA
AAAAGCAAGTGTATCACGCTTTGTACAAGCTAACTACACACCTTATGATGGAGACGAAAGCTTCCTTGCAGGACCAACAG
AGCGTTCACTTCACATCAAGAAAATTGTAGAAGAAACTAAAGCACACTACGAAGAAACTCGTTTCCCAATGGACACTCGT
CCAACATCTATCGCTGATATCCCTGCTGGATTTATCGACAAAGAAAATGAAGTTATCTTCGGTATCCAAAACGATGAACT
CTTCAAATTGAACTTCATGCCAAAAGGTGGTATCCGTATGGCTGAAACTACTTTGAAAGAAAATGGATACGAACCAGACC
CAGCTGTTCACGAAATCTTCACTAAATATGTAACAACAGTTAACGACGGTATTTTCCGTGCCTACACTTCAAATATTCGT
CGCGCTCGTCACGCACACACTGTAACTGGTCTTCCAGATGCATACTCACGCGGACGTATCATCGGTGTTTACGCACGTCT
TGCTCTTTACGGTGCAGACTACTTGATGCAAGAAAAAGTAAATGACTGGAATGCAATCAAAGAAATCGATGAAGAAACAA
TCCGTCTTCGTGAAGAAGTAAACCTTCAATACCAAGCATTGCAACAAGTTGTTCGCCTGGGTGACCTTTACGGGGTTGAT
GTTCGCAAACCAGCGATGAACGTGAAAGAAGCAATCCAATGGGTTAACATTGCTTTCATGGCTGTCTGCCGTGTGATTAA
CGGTGCTGCTACATCTCTAGGTCGTGTACCAATCGTATTGGACATCTTTGCAGAACGTGACCTTGCTCGTGGTACATTTA
CTGAATCAGAAATCCAAGAATTCGTTGATGATTTCGTTATGAAACTTCGTACAGTTAAATTTGCTCGTACAAAAGCTTAT
GACCAATTGTACTCAGGTGACCCAACCTTTATCACAACTTCTATGGCTGGTATGGGTAACGACGGTCGTCACCGTGTTAC
TAAGATGGACTACCGTTTCTTGAACACTCTTGACAACATCGGTAACTCTCCAGAGCCAAACTTGACAGTTCTTTGGACTG
ACAAATTGCCATACAACTTCCGTCGCTACTGTATGCACATGAGCCACAAACACTCTTCTATCCAATACGAAGGTGTAACA
ACAATGGCTAAAGACGGATATGGTGAAATGAGCTGTATCTCATGCTGTGTGTCTCCACTTGATCCAGAAAATGAAGAACA
ACGCCACAACATCCAGTACTTCGGTGCTCGTGTAAACGTTCTTAAAGCCCTTCTTACTGGTTTGAATGGTGGTTACGACG
ATGTTCACAAAGACTACAAAGTATTTGACATCGATCCTATTCGTGACGAAGTTCTTGAATTCGAATCAGTTAAAGCGAAC
TTTGAAAAATCTCTTGACTGGTTGACTGACACTTACGTAGATGCCTTGAACATCATCCACTACATGACTGATAAGTACAA
CTACGAAGCTGTTCAAATGGCCTTCTTGCCAACTAAACAACGTGCCAACATGGGATTCGGTATTTGTGGATTTGCTAACA
CTGTTGATACATTGTCAGCGATTAAATACGCTACAGTTAAACCAATCCGTGATGAAGATGGCTACATCTACGATTACGAA
ACAATCGGTGACTACCCACGCTGGGGTGAAGATGACCCACGTTCAAACGAATTGGCAGAATGGTTGATCGAAGCTTACAC
AACTCGTCTACGTAGCCACAAACTATACAAAGACGCAGAAGCTACAGTATCACTTTTGACAATCACATCTAACGTTGCTT
ACTCTAAACAAACTGGTAACTCACCAGTTCACAAAGGTGTATACCTCAACGAAGATGGTTCTGTGAACTTGTCTAAACTT
GAATTCTTCTCACCAGGTGCTAACCCATCTAACAAAGCTAAAGGTGGTTGGTTGCAAAACTTGAACTCACTTTCTAGCCT
TGACTTTAGTTATGCAGCTGACGGTATCTCATTGACTACACAAGTATCACCTCGCGCTCTTGGTAAGACTCGTGATGAAC
AAGTTGATAACTTGGTAACAATCCTTGATGGTTACTTCGAAAACGGTGGACAACACGTTAACTTGAACGTTATGGACTTG
AACGATGTTTACGAAAAAATCATGTCAGGCGAAGACGTTATCGTACGTATCTCTGGATACTGTGTAAACACTAAATACCT
CACTCCAGAACAAAAAACTGAATTGACACAACGTGTCTTCCACGAAGTTCTTTCAATGGATGACGCCTTGGATGCATTGA
GCTAA

Upstream 100 bases:

>100_bases
AAAAATAGGGATTTTTCACTTGTTGAAATCGGCTACTATATGGTATACTTGTCTCATGAATGTAACAGATGACTGTTACT
AGAAAAAAGAGGACATTAAT

Downstream 100 bases:

>100_bases
TCAAGTTCTTGAATAATAAAAAGGGCTCTTTGTCAACTGTAGTGGGTTGAAGAAAAGCTAAGCTCGAGAAAGGACAAATT
TTGTCCTTTCTTTTTTGATG

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 774; Mature: 774

Protein sequence:

>774_residues
MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDGDESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTR
PTSIADIPAGFIDKENEVIFGIQNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLREEVNLQYQALQQVVRLGDLYGVD
VRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAY
DQLYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHSSIQYEGVT
TMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGYDDVHKDYKVFDIDPIRDEVLEFESVKAN
FEKSLDWLTDTYVDALNIIHYMTDKYNYEAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYE
TIGDYPRWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVHKGVYLNEDGSVNLSKL
EFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQVSPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDL
NDVYEKIMSGEDVIVRISGYCVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS

Sequences:

>Translated_774_residues
MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDGDESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTR
PTSIADIPAGFIDKENEVIFGIQNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLREEVNLQYQALQQVVRLGDLYGVD
VRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAY
DQLYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHSSIQYEGVT
TMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGYDDVHKDYKVFDIDPIRDEVLEFESVKAN
FEKSLDWLTDTYVDALNIIHYMTDKYNYEAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYE
TIGDYPRWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVHKGVYLNEDGSVNLSKL
EFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQVSPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDL
NDVYEKIMSGEDVIVRISGYCVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS
>Mature_774_residues
MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDGDESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTR
PTSIADIPAGFIDKENEVIFGIQNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLREEVNLQYQALQQVVRLGDLYGVD
VRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAY
DQLYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHSSIQYEGVT
TMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGYDDVHKDYKVFDIDPIRDEVLEFESVKAN
FEKSLDWLTDTYVDALNIIHYMTDKYNYEAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYE
TIGDYPRWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVHKGVYLNEDGSVNLSKL
EFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQVSPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDL
NDVYEKIMSGEDVIVRISGYCVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=767, Percent_Identity=43.8070404172099, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI48994926, Length=771, Percent_Identity=41.2451361867704, Blast_Score=576, Evalue=1e-165,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 87781; Mature: 87781

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDGDESFLAGPTERSLHIK
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH
KIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGIQNDELFKLNFMPKGGIRM
HHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCH
AETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRARHAHTVTGLPDAYSRGRI
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCE
IGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLREEVNLQYQALQQVVRLGDLYGVD
EHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
VRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTESEIQE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCCCCCCCCHHHHHH
FVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDNI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHC
GNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHSSIQYEGVTTMAKDGYGEMSCISCCVSPL
CCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEECCCEEHHCCCCCCHHHHHHHCCCC
DPENEEQRHNIQYFGARVNVLKALLTGLNGGYDDVHKDYKVFDIDPIRDEVLEFESVKAN
CCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEECCHHHHHHHHHHHHHHH
FEKSLDWLTDTYVDALNIIHYMTDKYNYEAVQMAFLPTKQRANMGFGICGFANTVDTLSA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCCEEECCCHHHHHHHHH
IKYATVKPIRDEDGYIYDYETIGDYPRWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAE
HHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
ATVSLLTITSNVAYSKQTGNSPVHKGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQN
HEEEEEEEECCCEECCCCCCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHH
LNSLSSLDFSYAADGISLTTQVSPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDL
HHHHHHCCHHHCCCCEEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEH
NDVYEKIMSGEDVIVRISGYCVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS
HHHHHHHHCCCCEEEEEEEEEEECEECCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDGDESFLAGPTERSLHIK
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH
KIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGIQNDELFKLNFMPKGGIRM
HHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCH
AETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRARHAHTVTGLPDAYSRGRI
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCE
IGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLREEVNLQYQALQQVVRLGDLYGVD
EHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
VRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTESEIQE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCCCCCCCCHHHHHH
FVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDNI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHC
GNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHSSIQYEGVTTMAKDGYGEMSCISCCVSPL
CCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEECCCEEHHCCCCCCHHHHHHHCCCC
DPENEEQRHNIQYFGARVNVLKALLTGLNGGYDDVHKDYKVFDIDPIRDEVLEFESVKAN
CCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEECCHHHHHHHHHHHHHHH
FEKSLDWLTDTYVDALNIIHYMTDKYNYEAVQMAFLPTKQRANMGFGICGFANTVDTLSA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCCEEECCCHHHHHHHHH
IKYATVKPIRDEDGYIYDYETIGDYPRWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAE
HHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
ATVSLLTITSNVAYSKQTGNSPVHKGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQN
HEEEEEEEECCCEECCCCCCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHH
LNSLSSLDFSYAADGISLTTQVSPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDL
HHHHHHCCHHHCCCCEEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEH
NDVYEKIMSGEDVIVRISGYCVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS
HHHHHHHHCCCCEEEEEEEEEEECEECCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550181; 12397186 [H]