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The map label for this gene is ilvB [H]

Identifier: 169833081

GI number: 169833081

Start: 498402

End: 500102

Strand: Direct

Name: ilvB [H]

Synonym: SPH_0551

Alternate gene names: 169833081

Gene position: 498402-500102 (Clockwise)

Preceding gene: 169832623

Following gene: 169834441

Centisome position: 22.19

GC content: 44.44

Gene sequence:

>1701_bases
ATGGAGAAAATCAGTTTAGAATCTCCTAAGACGGGGTCGGACCTAGTTTTGGAAACACTTCGTGATTTAGGAGTTGATAC
CATCTTTGGTTATCCTGGTGGTGCGGTTTTGCCTTTTTATGATGCGATATATAATTTTAAAGGCATTCGCCACATTCTAG
GGCGCCATGAGCAAGGTTGTTTGCATGAAGCTGAAGGTTATGCCAAATCAACTGGAAAGTTGGGTGTTGCCGTCGTCACT
AGTGGACCAGGAGCAACAAATGCCATTACAGGGATTGCGGATGCCATGAGCGATAGCGTTCCCCTCTTGGTCTTTACAGG
TCAGGTGGCGCGAGCAGGGATTGGGAAGGATGCCTTTCAGGAGGCAGACATCGTGGGAATTACCATGCCAATCACTAAGT
ACAATTACCAAGTTCGTGAGACAGCTGATATTCCGCGTATCATTACGGAAGCTGTCCATATCGCAACTACAGGCCGTCCA
GGGCCAGTTGTAATTGACCTACCAAAAGACATATCTGCTTTAGAAACAGACTTCATTTATTCACCAGAAGTGAATTTACC
AAGTTATCAGCCGACTCTTGAGCCGAATGATATGCAAATCAAGAAAATCTTGAAGCAATTGTCCAAGGCTAAAAAGCCAG
TCTTGTTAGCTGGTGGTGGAATTAGTTATGCTGAGGCTGCTACGGAACTAAGTGAATTTGCAGAACGCTATCAAATTCCA
GTGGTAACCAGTCTTTTGGGACAAGGAACGATTGCAACGAGTCACCCACTCTTTCTTGGAATGGGAGGCATGCACGGGTC
ATTCGCAGCAAATATTGCCATGACGGAAGCGGACTTTATGATTAGTATTGGTTCTCGTTTCGATGACCGTTTGACGGGGA
ATCCTAAGACTTTCGCTAAGAATGCTAAGGTTGCCCACATTGATATTGACCCAGCTGAGATTGGCAAGATTATCAGTGCA
GACATTCCTGTAGTTGGAGATGCTAAGAAGGCCTTGCAAATGTTGCTAGCAGAACCAACAGTTCACAACAATACTGAAAA
GTGGATTGAGAAAGTCACTAAAGACAAGAATCGTGTTCGTTCTTATGACAAGAAAGAGCGTGTGGTTCAACCGCAAGCAG
TTATTGAACGAATTGGTGAATTGACGAATGGAGATGCCATTGTGGTAACAGACGTTGGTCAACACCAAATGTGGACAGCT
CAGTATTATCCCTACCAAAATGAGCGTCAGTTAGTGACTTCAGGTGGTTTGGGAACAATGGGCTTTGGAATTCCAGCAGC
AATCGGTGCTAAAATTGCTAACCCAGATAAGGAAGTAGTCTTGTTTGTTGGGGATGGTGGTTTCCAAATGACCAACCAGG
AGTTGGCTATTTTGAATATTTACAAGGTGCCAATCAAGGTGGTTATGCTGAACAATCATTCACTCGGAATGGTTCGCCAG
TGGCAGGAATCCTTCTATGAAGGCAGAACATCAGAGTCGGTCTTTGATACCCTTCCTGATTTCCAATTGATGGCGCAGGC
TTATGGTATTAAAAACTATAAGTTTGACAATCCTGAGACCTTGGCTCAAGACCTTGAAGTCATCACTGAGGATGTTCCTA
TGCTAATTGAGGTAGATATTTCTCGTAAGGAACAGGTGTTACCAATGGTACCGGCTGGTAAGAGTAATCATGAGATGTTG
GGGGTGAAGTTCCATGCGTAG

Upstream 100 bases:

>100_bases
AAAATAGAAAATTCAGAAAATTTCTTCTTTTATCTTGAAAAATTTTGAAAAAATGGTATGATAGTAACAAGTTATTTTTA
AGAGGAAAGAAAGGGGAACA

Downstream 100 bases:

>100_bases
AATGTTAACAGCAAAACTACAAAATCGTTCAGGAGTCCTCAATCGCTTTACAGGTGTCCTATCTCGTCGTCAGGTTAATA
TTGAAAGCATCTCTGTTGGA

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIYNFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVT
SGPGATNAITGIADAMSDSVPLLVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAGGGISYAEAATELSEFAERYQIP
VVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTEADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISA
DIPVVGDAKKALQMLLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDVGQHQMWTA
QYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQMTNQELAILNIYKVPIKVVMLNNHSLGMVRQ
WQESFYEGRTSESVFDTLPDFQLMAQAYGIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEML
GVKFHA

Sequences:

>Translated_566_residues
MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIYNFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVT
SGPGATNAITGIADAMSDSVPLLVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAGGGISYAEAATELSEFAERYQIP
VVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTEADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISA
DIPVVGDAKKALQMLLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDVGQHQMWTA
QYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQMTNQELAILNIYKVPIKVVMLNNHSLGMVRQ
WQESFYEGRTSESVFDTLPDFQLMAQAYGIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEML
GVKFHA
>Mature_566_residues
MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIYNFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVT
SGPGATNAITGIADAMSDSVPLLVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAGGGISYAEAATELSEFAERYQIP
VVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTEADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISA
DIPVVGDAKKALQMLLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDVGQHQMWTA
QYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQMTNQELAILNIYKVPIKVVMLNNHSLGMVRQ
WQESFYEGRTSESVFDTLPDFQLMAQAYGIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEML
GVKFHA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=573, Percent_Identity=25.8289703315881, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI21361361, Length=519, Percent_Identity=24.6628131021195, Blast_Score=153, Evalue=4e-37,
Organism=Escherichia coli, GI87081685, Length=562, Percent_Identity=46.2633451957295, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI1790104, Length=556, Percent_Identity=41.1870503597122, Blast_Score=430, Evalue=1e-121,
Organism=Escherichia coli, GI1786717, Length=555, Percent_Identity=35.6756756756757, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1787096, Length=552, Percent_Identity=27.536231884058, Blast_Score=209, Evalue=5e-55,
Organism=Escherichia coli, GI1788716, Length=480, Percent_Identity=27.5, Blast_Score=171, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17531299, Length=487, Percent_Identity=29.9794661190965, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17531301, Length=487, Percent_Identity=29.9794661190965, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17542570, Length=531, Percent_Identity=24.105461393597, Blast_Score=138, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=575, Percent_Identity=42.4347826086957, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6320816, Length=475, Percent_Identity=24.2105263157895, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320123, Length=548, Percent_Identity=22.8102189781022, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=569, Percent_Identity=24.0773286467487, Blast_Score=110, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6323073, Length=478, Percent_Identity=24.2677824267782, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321524, Length=561, Percent_Identity=24.2424242424242, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922626, Length=568, Percent_Identity=26.9366197183099, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61665; Mature: 61665

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIYNFKGIRHILGRHEQGC
CCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
LHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPLLVFTGQVARAGIGKDAFQ
HHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHC
EADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRPGPVVIDLPKDISALETDFIY
CCCEEEEEEEEEECCEEEECCCCHHHHHHHHHHEEECCCCCCEEEECCCCCHHHHHCEEE
SPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAGGGISYAEAATELSEFAERYQIP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCC
VVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTEADFMISIGSRFDDRLTGNPKTFAK
HHHHHHCCCCEEECCCEEEEECCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCHHHHC
NAKVAHIDIDPAEIGKIISADIPVVGDAKKALQMLLAEPTVHNNTEKWIEKVTKDKNRVR
CCEEEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
SYDKKERVVQPQAVIERIGELTNGDAIVVTDVGQHQMWTAQYYPYQNERQLVTSGGLGTM
CCCHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCEEEECCCCCCC
GFGIPAAIGAKIANPDKEVVLFVGDGGFQMTNQELAILNIYKVPIKVVMLNNHSLGMVRQ
CCCCHHHHCCCCCCCCCEEEEEEECCCCEECCCCEEEEEEEECCEEEEEECCCCCHHHHH
WQESFYEGRTSESVFDTLPDFQLMAQAYGIKNYKFDNPETLAQDLEVITEDVPMLIEVDI
HHHHHHCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEC
SRKEQVLPMVPAGKSNHEMLGVKFHA
CCCCCCCEECCCCCCCCEEEEEEECC
>Mature Secondary Structure
MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIYNFKGIRHILGRHEQGC
CCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
LHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPLLVFTGQVARAGIGKDAFQ
HHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHC
EADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRPGPVVIDLPKDISALETDFIY
CCCEEEEEEEEEECCEEEECCCCHHHHHHHHHHEEECCCCCCEEEECCCCCHHHHHCEEE
SPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAGGGISYAEAATELSEFAERYQIP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCC
VVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTEADFMISIGSRFDDRLTGNPKTFAK
HHHHHHCCCCEEECCCEEEEECCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCHHHHC
NAKVAHIDIDPAEIGKIISADIPVVGDAKKALQMLLAEPTVHNNTEKWIEKVTKDKNRVR
CCEEEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
SYDKKERVVQPQAVIERIGELTNGDAIVVTDVGQHQMWTAQYYPYQNERQLVTSGGLGTM
CCCHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCEEEECCCCCCC
GFGIPAAIGAKIANPDKEVVLFVGDGGFQMTNQELAILNIYKVPIKVVMLNNHSLGMVRQ
CCCCHHHHCCCCCCCCCEEEEEEECCCCEECCCCEEEEEEEECCEEEEEECCCCCHHHHH
WQESFYEGRTSESVFDTLPDFQLMAQAYGIKNYKFDNPETLAQDLEVITEDVPMLIEVDI
HHHHHHCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEC
SRKEQVLPMVPAGKSNHEMLGVKFHA
CCCCCCCEECCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400210; 11337471 [H]