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The map label for this gene is potB [H]

Identifier: 169824261

GI number: 169824261

Start: 626425

End: 627234

Strand: Direct

Name: potB [H]

Synonym: FMG_0564

Alternate gene names: 169824261

Gene position: 626425-627234 (Clockwise)

Preceding gene: 169824260

Following gene: 169824262

Centisome position: 34.85

GC content: 29.75

Gene sequence:

>810_bases
ATGAAGCTTAAGAAATTATCGATTCCTTACGCTATTTGGATATTATTTTTCATTGTATTTCCATTGTTTCTTATTGTTTA
TTATTCAGTTACAACTGGATCATTGAGAGATTTTTCCAATCCACAATTTTCACTTGAATATTTTTACAGATTTTTTACAT
CGAAATATGTAAAAGTTCTTTTGAACTCTATTAGATTAGCATTTATAAGTACAGTAATTTGTTTGTTGATTGGATATCCG
ACAGCTTATTTTATCAGTAAAGTAGACAAAAAATACAAGGCTGTATTGATGCTTATGATAGTTATTCCGATGTGGATGAA
CTTTTTATTGAGAACTTATGCATGGATTATTTTAACTGGTAAAAACGGAATAATTAACAGTGTGTTAATTCATTTTGGTT
TTTCTCCCATTAAAATGCTATACACTGAAGGAGCTATTTTAATGGGGATGGTTTACAATTTCCTACCTTTCATGATACTT
CCTATTTTCTCAATTTTGGATAAATCAGATTATTCATTAGTAGAAGCAAGCTATGATTTGGGAGCATCTAGGTTACAAAC
TTTTTTACGAGTTGTATTTCCAATGAGTTTACCAGGGGTAATTACAGGAATTACTATGGTATTTATTCCAGCGATTTCTA
CATTCGAAATATCGGCATTGCTTGGTGGAAACAAATACAATTTGATAGGAAATATAATTGAGCATCAATTTAGGATAGCT
GGAAATTGGCATTACGGTAGTGCAATTTCTGTAGTACTTATAATTATGATTTTAATTTCATTAGCTTTTACGAATGAGAA
GGGAGAATAA

Upstream 100 bases:

>100_bases
TTCAAGTATAAGGCTCAATCAACAATTAATAAACCTCAAGGAAGTATGGTAGGAATTAACATTGATCCAGATAATATTCA
TATAATGAAGAGGTGCGACG

Downstream 100 bases:

>100_bases
AATGAAAGAATTTTTCAAGAAATTTTATTTAGCATTGGTATTTTTATTCCTTTACGGACCAATTGCTGTTTTGATGGTAT
TTTCATTTAACGATTCTCGT

Product: spermidine/putrescine ABC transporter permease

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MKLKKLSIPYAIWILFFIVFPLFLIVYYSVTTGSLRDFSNPQFSLEYFYRFFTSKYVKVLLNSIRLAFISTVICLLIGYP
TAYFISKVDKKYKAVLMLMIVIPMWMNFLLRTYAWIILTGKNGIINSVLIHFGFSPIKMLYTEGAILMGMVYNFLPFMIL
PIFSILDKSDYSLVEASYDLGASRLQTFLRVVFPMSLPGVITGITMVFIPAISTFEISALLGGNKYNLIGNIIEHQFRIA
GNWHYGSAISVVLIIMILISLAFTNEKGE

Sequences:

>Translated_269_residues
MKLKKLSIPYAIWILFFIVFPLFLIVYYSVTTGSLRDFSNPQFSLEYFYRFFTSKYVKVLLNSIRLAFISTVICLLIGYP
TAYFISKVDKKYKAVLMLMIVIPMWMNFLLRTYAWIILTGKNGIINSVLIHFGFSPIKMLYTEGAILMGMVYNFLPFMIL
PIFSILDKSDYSLVEASYDLGASRLQTFLRVVFPMSLPGVITGITMVFIPAISTFEISALLGGNKYNLIGNIIEHQFRIA
GNWHYGSAISVVLIIMILISLAFTNEKGE
>Mature_269_residues
MKLKKLSIPYAIWILFFIVFPLFLIVYYSVTTGSLRDFSNPQFSLEYFYRFFTSKYVKVLLNSIRLAFISTVICLLIGYP
TAYFISKVDKKYKAVLMLMIVIPMWMNFLLRTYAWIILTGKNGIINSVLIHFGFSPIKMLYTEGAILMGMVYNFLPFMIL
PIFSILDKSDYSLVEASYDLGASRLQTFLRVVFPMSLPGVITGITMVFIPAISTFEISALLGGNKYNLIGNIIEHQFRIA
GNWHYGSAISVVLIIMILISLAFTNEKGE

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=247, Percent_Identity=38.0566801619433, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1787080, Length=216, Percent_Identity=37.037037037037, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1787713, Length=253, Percent_Identity=29.6442687747036, Blast_Score=120, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30695; Mature: 30695

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKKLSIPYAIWILFFIVFPLFLIVYYSVTTGSLRDFSNPQFSLEYFYRFFTSKYVKVL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHH
LNSIRLAFISTVICLLIGYPTAYFISKVDKKYKAVLMLMIVIPMWMNFLLRTYAWIILTG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
KNGIINSVLIHFGFSPIKMLYTEGAILMGMVYNFLPFMILPIFSILDKSDYSLVEASYDL
CCCHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
GASRLQTFLRVVFPMSLPGVITGITMVFIPAISTFEISALLGGNKYNLIGNIIEHQFRIA
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEE
GNWHYGSAISVVLIIMILISLAFTNEKGE
CCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLKKLSIPYAIWILFFIVFPLFLIVYYSVTTGSLRDFSNPQFSLEYFYRFFTSKYVKVL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHH
LNSIRLAFISTVICLLIGYPTAYFISKVDKKYKAVLMLMIVIPMWMNFLLRTYAWIILTG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
KNGIINSVLIHFGFSPIKMLYTEGAILMGMVYNFLPFMILPIFSILDKSDYSLVEASYDL
CCCHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
GASRLQTFLRVVFPMSLPGVITGITMVFIPAISTFEISALLGGNKYNLIGNIIEHQFRIA
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEE
GNWHYGSAISVVLIIMILISLAFTNEKGE
CCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]