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The map label for this gene is ispE

Identifier: 169824249

GI number: 169824249

Start: 608377

End: 609213

Strand: Direct

Name: ispE

Synonym: FMG_0552

Alternate gene names: 169824249

Gene position: 608377-609213 (Clockwise)

Preceding gene: 169824248

Following gene: 169824250

Centisome position: 33.84

GC content: 30.23

Gene sequence:

>837_bases
TTGATGAAGATTAATAGTTACGCAAAGATAAATCTATCGTTAGATGTTCTTGATTTAAGGGAAGATGGATATCATAACAT
CGACACAATTATGAACCTGATTGATCTGCACGATGTTATAGAAATAAATCGTAATAATACTAATGAGTTAAAACTTAGCT
CCAACAATTCGAATTTTCCTACTGATAAAACTAATTTGATATATAAAATATTTGAAAATTTAAAAAAGTTTAGAAAAATC
AACTGTAGCTATGATGTGTTTGTAGATAAACAAATTCCTATATCAGCGGGACTTGCAGGAGGAAGCTCCAATGCATGCGA
GTTTTTGATGTATGTTAATGATGATTTGTCGTTAAATCTTTCAAATCAAGAAATCAAGGAAATCTGCAGATTAACAGGAG
CAGACACTTTCTATTTTACGAGCAAAAAATGTGTCAGAGCAACAGGAATTGGAAATGAGTTTGTTAGACTATCTGATTTT
TCTAATAAAAACATAATAATTGTAAATAACGGAATGAGTATATCTTCAAAAGATGTGTATGATAATTTGAAAGAATCAAA
CGGTGGACTTGGTAAAATTACTGTTGCAATTGATAGCAGAGATTATGAAACTTTTTACAGGAAAGCATTTAACACGATGG
AAAGTGTATCAGAAAGATTAGTCGAAGAAATCTCTGATATAAAGGAACAGTTGAACAATCTCGGCTGTGATTTGTCACTT
ATGAGTGGATCGGGACCAACTGTTTTTGGGATTTTTGAAAATTATAATGACTATGAGAATTGCTACAGTAAATTAAAGGA
TAAATATAAGTATGTATTCAAAACAAAAACCTTATAA

Upstream 100 bases:

>100_bases
GAATTCGAATCAATTGTAAAGGACAACAAAGTAGGAGTTCCTATGATAACTGATGGCAAAAAGATAGTATTTTTTGAAAA
ATTAGAAGATTTAAGAGTGT

Downstream 100 bases:

>100_bases
CAAAATAGGATTCGTGGATTCTGGATTGGGCGGACTTACAGTATTCAAAAAAGTAAAAAAACTGATGCCCAATAAGGAGT
ATATTTACTTTGCTGATAGT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MMKINSYAKINLSLDVLDLREDGYHNIDTIMNLIDLHDVIEINRNNTNELKLSSNNSNFPTDKTNLIYKIFENLKKFRKI
NCSYDVFVDKQIPISAGLAGGSSNACEFLMYVNDDLSLNLSNQEIKEICRLTGADTFYFTSKKCVRATGIGNEFVRLSDF
SNKNIIIVNNGMSISSKDVYDNLKESNGGLGKITVAIDSRDYETFYRKAFNTMESVSERLVEEISDIKEQLNNLGCDLSL
MSGSGPTVFGIFENYNDYENCYSKLKDKYKYVFKTKTL

Sequences:

>Translated_278_residues
MMKINSYAKINLSLDVLDLREDGYHNIDTIMNLIDLHDVIEINRNNTNELKLSSNNSNFPTDKTNLIYKIFENLKKFRKI
NCSYDVFVDKQIPISAGLAGGSSNACEFLMYVNDDLSLNLSNQEIKEICRLTGADTFYFTSKKCVRATGIGNEFVRLSDF
SNKNIIIVNNGMSISSKDVYDNLKESNGGLGKITVAIDSRDYETFYRKAFNTMESVSERLVEEISDIKEQLNNLGCDLSL
MSGSGPTVFGIFENYNDYENCYSKLKDKYKYVFKTKTL
>Mature_278_residues
MMKINSYAKINLSLDVLDLREDGYHNIDTIMNLIDLHDVIEINRNNTNELKLSSNNSNFPTDKTNLIYKIFENLKKFRKI
NCSYDVFVDKQIPISAGLAGGSSNACEFLMYVNDDLSLNLSNQEIKEICRLTGADTFYFTSKKCVRATGIGNEFVRLSDF
SNKNIIIVNNGMSISSKDVYDNLKESNGGLGKITVAIDSRDYETFYRKAFNTMESVSERLVEEISDIKEQLNNLGCDLSL
MSGSGPTVFGIFENYNDYENCYSKLKDKYKYVFKTKTL

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=275, Percent_Identity=25.4545454545455, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_FINM2 (B0S057)

Other databases:

- EMBL:   AP008971
- RefSeq:   YP_001691860.1
- ProteinModelPortal:   B0S057
- SMR:   B0S057
- GeneID:   6019573
- GenomeReviews:   AP008971_GR
- KEGG:   fma:FMG_0552
- HOGENOM:   HBG734593
- OMA:   DGYHNID
- BioCyc:   FMAG334413:FMG_0552-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31631; Mature: 31631

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: ACT_SITE 9-9 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKINSYAKINLSLDVLDLREDGYHNIDTIMNLIDLHDVIEINRNNTNELKLSSNNSNFP
CCEECCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHEEECCCCCCEEEECCCCCCCC
TDKTNLIYKIFENLKKFRKINCSYDVFVDKQIPISAGLAGGSSNACEFLMYVNDDLSLNL
CCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCEEEE
SNQEIKEICRLTGADTFYFTSKKCVRATGIGNEFVRLSDFSNKNIIIVNNGMSISSKDVY
CHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCEEEEECCCCCCEEEEECCCCCCCHHHH
DNLKESNGGLGKITVAIDSRDYETFYRKAFNTMESVSERLVEEISDIKEQLNNLGCDLSL
HHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
MSGSGPTVFGIFENYNDYENCYSKLKDKYKYVFKTKTL
ECCCCCEEEEEECCCCCHHHHHHHHHHHHEEEEEECCC
>Mature Secondary Structure
MMKINSYAKINLSLDVLDLREDGYHNIDTIMNLIDLHDVIEINRNNTNELKLSSNNSNFP
CCEECCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHEEECCCCCCEEEECCCCCCCC
TDKTNLIYKIFENLKKFRKINCSYDVFVDKQIPISAGLAGGSSNACEFLMYVNDDLSLNL
CCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCEEEE
SNQEIKEICRLTGADTFYFTSKKCVRATGIGNEFVRLSDFSNKNIIIVNNGMSISSKDVY
CHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCEEEEECCCCCCEEEEECCCCCCCHHHH
DNLKESNGGLGKITVAIDSRDYETFYRKAFNTMESVSERLVEEISDIKEQLNNLGCDLSL
HHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
MSGSGPTVFGIFENYNDYENCYSKLKDKYKYVFKTKTL
ECCCCCEEEEEECCCCCHHHHHHHHHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA