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The map label for this gene is ansB [H]

Identifier: 169824218

GI number: 169824218

Start: 586595

End: 588118

Strand: Direct

Name: ansB [H]

Synonym: FMG_0521

Alternate gene names: 169824218

Gene position: 586595-588118 (Clockwise)

Preceding gene: 169824217

Following gene: 169824219

Centisome position: 32.63

GC content: 33.53

Gene sequence:

>1524_bases
GTGTTAATATCAATTTGTAATGTAATAAATAAACGTTTTTTGGGGGGTAATATGGATTATAGATTAGAGTCTGATAGTGT
TGGAGAAAAACAAGTTCCAAAAGATGCATATTACGGTGTGCAATCTTTAAGAGGAATGGAAAATTTCCCGATAACAGGAA
GAACTTTACATGAAGAATTAATTTATGGATTGACAATAGTTAAAAAAGCTTGTGCAATTGCAAACCACAGAGCAGGAATT
ATGAGAGAGGATGTCAAAAACGCAATCGTTTACGCATGTGACCAAATTCTTAAAGGAGAATATCACGATCAATTTTTGAC
TGATCCAATTCAAGGTGGAGCAGGTACAACAGCTAATATGAATGCAAATGAAGTTATTGCAAACATTGCCAACGAAAAAC
TCGGTGGAGGATTGGGAACTTATGAATATGTTCACCCAAACGATCACGTTAACATGAGTCAATCTACAAACGATGTTTTT
CCTACTGCGGGTAAAATTGCAACTTTAAGACTACTAATAAAAGCTTTAAACGAATTAAAGCAATTAGAAATTTCTTTGCT
TAATAAATCTGTAGAATTTGATGATGTACTTAAAATGGGACGTACTCAACTTCAAGATGCAGTGCCAATTAGATTGGGAC
AAGAATTCCACGCGTATTATTCAGTAATCAAAAGAGATATCAAAAGAATTAGAAGATCATCAGATCAAATTGAATCCATA
AATATGGGAGCAACTGCGATTGGTACAGGTGTAAATGTAGACAAAGTTTACTTTACAAATATAGTTCCGATTCTAGCAGA
GTTAACAGGATTTGATTTGCATCAAGCAGAAGATTTAATAGATGGAACTAATAACCTTGATGGACTTGTAGAAATATCTT
CAGCAGTAAAATCAACAGCTGTAAGTTTGTCAAAAATGGCAAGTGATTTGAGATTAATGAGTTCTGGACCAAAAACAATG
GTAGGAGATATTGAACTTCCACCAAGACAAAATGGTTCATCAATTATGCCAGGTAAAGTAAATCCTGTAATTCCAGAAGT
TGTTAACCAAGTTGTATTCAGAATTATTGGTAACGATAATACAGTTACAATGTGTGCAGAACACGGACAATTGGAACTTA
ACGCATTCGAACCAGTTTTGTTCGATAGTTTATTTGAATCAATATCTATATTGACAAATGCAGTGAGAACTTTCAGAGAA
AACTGCATAATTGGTATCAAACCAAACAGACAAAAACTTTCAAACGACGTCGAAAAATCAGTAGGAATAGTTACAGCGTT
GGTTCCTCATATTGGATACAAAAAGTCCGCTGAAATTGCAAAACGTGCATTGAGTACAGGAATCACAGTTAGAAATATCG
TATTGGATGAAAAAATCTTAACAGAAGATGAATTAAAAGAAATTCTTGATCCAATTGCGATGACAATGCCAGGAATTGCA
GCAGAATATTTGATTAAACAAAAACAACAAGCCTACATCGAAAAAAAGAAACTAGAAGACAAGGAACAAAAATCAAAAAA
ATAA

Upstream 100 bases:

>100_bases
ATCTATTATCAGCCGATAAAAAGTTTTTCAAACTTTGAAATTTCGGCTGATTTTTATTTTAAAATCAATTTTTATAAAAT
TTTGACAATGGATTTTAAAA

Downstream 100 bases:

>100_bases
ATTTGAGTTGACAAAATCTTGTTATCAAGTATTTGATAACAGCATTTTTTATTACGAACTAAAAGTTTTACATTATTTTA
CATTTGTTAATATGTTATAA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MLISICNVINKRFLGGNMDYRLESDSVGEKQVPKDAYYGVQSLRGMENFPITGRTLHEELIYGLTIVKKACAIANHRAGI
MREDVKNAIVYACDQILKGEYHDQFLTDPIQGGAGTTANMNANEVIANIANEKLGGGLGTYEYVHPNDHVNMSQSTNDVF
PTAGKIATLRLLIKALNELKQLEISLLNKSVEFDDVLKMGRTQLQDAVPIRLGQEFHAYYSVIKRDIKRIRRSSDQIESI
NMGATAIGTGVNVDKVYFTNIVPILAELTGFDLHQAEDLIDGTNNLDGLVEISSAVKSTAVSLSKMASDLRLMSSGPKTM
VGDIELPPRQNGSSIMPGKVNPVIPEVVNQVVFRIIGNDNTVTMCAEHGQLELNAFEPVLFDSLFESISILTNAVRTFRE
NCIIGIKPNRQKLSNDVEKSVGIVTALVPHIGYKKSAEIAKRALSTGITVRNIVLDEKILTEDELKEILDPIAMTMPGIA
AEYLIKQKQQAYIEKKKLEDKEQKSKK

Sequences:

>Translated_507_residues
MLISICNVINKRFLGGNMDYRLESDSVGEKQVPKDAYYGVQSLRGMENFPITGRTLHEELIYGLTIVKKACAIANHRAGI
MREDVKNAIVYACDQILKGEYHDQFLTDPIQGGAGTTANMNANEVIANIANEKLGGGLGTYEYVHPNDHVNMSQSTNDVF
PTAGKIATLRLLIKALNELKQLEISLLNKSVEFDDVLKMGRTQLQDAVPIRLGQEFHAYYSVIKRDIKRIRRSSDQIESI
NMGATAIGTGVNVDKVYFTNIVPILAELTGFDLHQAEDLIDGTNNLDGLVEISSAVKSTAVSLSKMASDLRLMSSGPKTM
VGDIELPPRQNGSSIMPGKVNPVIPEVVNQVVFRIIGNDNTVTMCAEHGQLELNAFEPVLFDSLFESISILTNAVRTFRE
NCIIGIKPNRQKLSNDVEKSVGIVTALVPHIGYKKSAEIAKRALSTGITVRNIVLDEKILTEDELKEILDPIAMTMPGIA
AEYLIKQKQQAYIEKKKLEDKEQKSKK
>Mature_507_residues
MLISICNVINKRFLGGNMDYRLESDSVGEKQVPKDAYYGVQSLRGMENFPITGRTLHEELIYGLTIVKKACAIANHRAGI
MREDVKNAIVYACDQILKGEYHDQFLTDPIQGGAGTTANMNANEVIANIANEKLGGGLGTYEYVHPNDHVNMSQSTNDVF
PTAGKIATLRLLIKALNELKQLEISLLNKSVEFDDVLKMGRTQLQDAVPIRLGQEFHAYYSVIKRDIKRIRRSSDQIESI
NMGATAIGTGVNVDKVYFTNIVPILAELTGFDLHQAEDLIDGTNNLDGLVEISSAVKSTAVSLSKMASDLRLMSSGPKTM
VGDIELPPRQNGSSIMPGKVNPVIPEVVNQVVFRIIGNDNTVTMCAEHGQLELNAFEPVLFDSLFESISILTNAVRTFRE
NCIIGIKPNRQKLSNDVEKSVGIVTALVPHIGYKKSAEIAKRALSTGITVRNIVLDEKILTEDELKEILDPIAMTMPGIA
AEYLIKQKQQAYIEKKKLEDKEQKSKK

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=462, Percent_Identity=40.4761904761905, Blast_Score=337, Evalue=2e-92,
Organism=Escherichia coli, GI87082375, Length=473, Percent_Identity=48.4143763213531, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1787896, Length=455, Percent_Identity=39.5604395604396, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1790398, Length=308, Percent_Identity=24.3506493506494, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17553882, Length=460, Percent_Identity=39.5652173913043, Blast_Score=341, Evalue=6e-94,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=37.9204892966361, Blast_Score=240, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6324993, Length=464, Percent_Identity=39.6551724137931, Blast_Score=345, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24640177, Length=463, Percent_Identity=40.3887688984881, Blast_Score=340, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24640179, Length=463, Percent_Identity=40.3887688984881, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24662684, Length=465, Percent_Identity=40, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=39.0928725701944, Blast_Score=332, Evalue=5e-91,
Organism=Drosophila melanogaster, GI24583245, Length=464, Percent_Identity=37.9310344827586, Blast_Score=311, Evalue=6e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 55948; Mature: 55948

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLISICNVINKRFLGGNMDYRLESDSVGEKQVPKDAYYGVQSLRGMENFPITGRTLHEEL
CEEHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHH
IYGLTIVKKACAIANHRAGIMREDVKNAIVYACDQILKGEYHDQFLTDPIQGGAGTTANM
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCC
NANEVIANIANEKLGGGLGTYEYVHPNDHVNMSQSTNDVFPTAGKIATLRLLIKALNELK
CHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
QLEISLLNKSVEFDDVLKMGRTQLQDAVPIRLGQEFHAYYSVIKRDIKRIRRSSDQIESI
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHCHHHHHHHHHHHHHHHHHHHCHHHHHHC
NMGATAIGTGVNVDKVYFTNIVPILAELTGFDLHQAEDLIDGTNNLDGLVEISSAVKSTA
CCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VSLSKMASDLRLMSSGPKTMVGDIELPPRQNGSSIMPGKVNPVIPEVVNQVVFRIIGNDN
HHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
TVTMCAEHGQLELNAFEPVLFDSLFESISILTNAVRTFRENCIIGIKPNRQKLSNDVEKS
EEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHH
VGIVTALVPHIGYKKSAEIAKRALSTGITVRNIVLDEKILTEDELKEILDPIAMTMPGIA
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHH
AEYLIKQKQQAYIEKKKLEDKEQKSKK
HHHHHHHHHHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure
MLISICNVINKRFLGGNMDYRLESDSVGEKQVPKDAYYGVQSLRGMENFPITGRTLHEEL
CEEHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHH
IYGLTIVKKACAIANHRAGIMREDVKNAIVYACDQILKGEYHDQFLTDPIQGGAGTTANM
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCC
NANEVIANIANEKLGGGLGTYEYVHPNDHVNMSQSTNDVFPTAGKIATLRLLIKALNELK
CHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
QLEISLLNKSVEFDDVLKMGRTQLQDAVPIRLGQEFHAYYSVIKRDIKRIRRSSDQIESI
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHCHHHHHHHHHHHHHHHHHHHCHHHHHHC
NMGATAIGTGVNVDKVYFTNIVPILAELTGFDLHQAEDLIDGTNNLDGLVEISSAVKSTA
CCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VSLSKMASDLRLMSSGPKTMVGDIELPPRQNGSSIMPGKVNPVIPEVVNQVVFRIIGNDN
HHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
TVTMCAEHGQLELNAFEPVLFDSLFESISILTNAVRTFRENCIIGIKPNRQKLSNDVEKS
EEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHH
VGIVTALVPHIGYKKSAEIAKRALSTGITVRNIVLDEKILTEDELKEILDPIAMTMPGIA
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHH
AEYLIKQKQQAYIEKKKLEDKEQKSKK
HHHHHHHHHHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]