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The map label for this gene is maeB [C]

Identifier: 169824196

GI number: 169824196

Start: 562588

End: 563760

Strand: Direct

Name: maeB [C]

Synonym: FMG_0499

Alternate gene names: 169824196

Gene position: 562588-563760 (Clockwise)

Preceding gene: 169824195

Following gene: 169824197

Centisome position: 31.3

GC content: 35.04

Gene sequence:

>1173_bases
ATGAATATTGAAGAAATTAAACAAAAAGCTCTTGAACTTCACGAAAAAAATCAAGGAAAATTAGAAGTAGTATCTAAAGT
AAAAGTTAATGACGCTATGGATTTGGCGTTGGCTTACACTCCTGGTGTTGCTGAACCTTGCAAGAAAATTCACGAAGACA
ATTCATTAGCATATAAATATACAAATAAAGGAAATATGGTTGCAGTTGTAACTGATGGTTCTGCAGTTTTAGGCTTAGGA
AATATTGGCGGAATTGCTGGTATGCCAGTTATGGAAGGTAAATCAGTTTTATTTAAAGAATTTGGTAATGTTGATGCATT
CCCAATTTGCTTGAACACACAAGACACTGAAGAAATAATTAGCACGGTAAAAGCAATTTCTCCTTCATTCGGTGGAATTA
ACCTTGAAGATATTGCAGCTCCAAGATGTTTCGAAATTGAAAAAAGATTAATTGAAGAATTAGATATACCAGTATTCCAC
GATGACCAACACGGTACAGCTGTTATCGTATTATCTGCAGTTATCAATGCGTTGAGAATTGTTAAGAAAGACATTTCTGA
TGTTAAAATCGTAATCAGTGGTGCAGGGGCAGCAGGTATTGCAATTTCTAACTTGTTAACATTGGCAGGTGCTAAAAATA
ATTTAATCTTAAATAGCCGTGGAATTATCGATCCAGATGATGAAAAACTTGATGAAGCTAGAAGAGAAATCGCTAGAACA
ACTAATCCTAATAAAGTTAAAGGAGATTTGAAAACTGCTATGGAAAATGCAGACATTTTCATCGGGGTTTCTGCTCCTGG
AATCTTAACAAAAGAAATGGTATCTTCTATGAATCACGATGCTATTGTTTTAGCAATGGCTAACCCAGTTCCAGAAATCT
ATCCTGACGAAGCAAAAGAAGCTGGTGCAAGAGTAGTTGGAACAGGTAGAAGTGATTATCCTAACCAAGTTAACAATGTT
TTGGTATTCCCTGGAATATTCAGAGGCGCATTAGATGCAAAAGCAGACAGAATTACTGACGATATGAAATTATCAGCAGC
GTATGCAATTGCTCACATGGTTGAACAACCAGTAGAAGAAAGAATTTTACCAGAAGCATTCAACAAAGATGTAGCAAAAA
ATGTTGCAAATGCAGTTAAAAAATCTTGGGAAGAAAAGAAAGTTATAAAATAG

Upstream 100 bases:

>100_bases
TTTTTGTTTGGAATAGTTGGGTTTTAGTGTTAAAATTATATTGTAATAATGTAATTCGACATTTTGTCGAAAGTTATTTA
AAAATTTATGAGGTGAAAAA

Downstream 100 bases:

>100_bases
GAGAAAACAATGATAGATATAGTTGATAGATTTATTGAATACGCAAAAATTTACACTACAAGTGATGAATCAAGCGAAAC
TTGTCCATCAACAGCTAGGC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MNIEEIKQKALELHEKNQGKLEVVSKVKVNDAMDLALAYTPGVAEPCKKIHEDNSLAYKYTNKGNMVAVVTDGSAVLGLG
NIGGIAGMPVMEGKSVLFKEFGNVDAFPICLNTQDTEEIISTVKAISPSFGGINLEDIAAPRCFEIEKRLIEELDIPVFH
DDQHGTAVIVLSAVINALRIVKKDISDVKIVISGAGAAGIAISNLLTLAGAKNNLILNSRGIIDPDDEKLDEARREIART
TNPNKVKGDLKTAMENADIFIGVSAPGILTKEMVSSMNHDAIVLAMANPVPEIYPDEAKEAGARVVGTGRSDYPNQVNNV
LVFPGIFRGALDAKADRITDDMKLSAAYAIAHMVEQPVEERILPEAFNKDVAKNVANAVKKSWEEKKVIK

Sequences:

>Translated_390_residues
MNIEEIKQKALELHEKNQGKLEVVSKVKVNDAMDLALAYTPGVAEPCKKIHEDNSLAYKYTNKGNMVAVVTDGSAVLGLG
NIGGIAGMPVMEGKSVLFKEFGNVDAFPICLNTQDTEEIISTVKAISPSFGGINLEDIAAPRCFEIEKRLIEELDIPVFH
DDQHGTAVIVLSAVINALRIVKKDISDVKIVISGAGAAGIAISNLLTLAGAKNNLILNSRGIIDPDDEKLDEARREIART
TNPNKVKGDLKTAMENADIFIGVSAPGILTKEMVSSMNHDAIVLAMANPVPEIYPDEAKEAGARVVGTGRSDYPNQVNNV
LVFPGIFRGALDAKADRITDDMKLSAAYAIAHMVEQPVEERILPEAFNKDVAKNVANAVKKSWEEKKVIK
>Mature_390_residues
MNIEEIKQKALELHEKNQGKLEVVSKVKVNDAMDLALAYTPGVAEPCKKIHEDNSLAYKYTNKGNMVAVVTDGSAVLGLG
NIGGIAGMPVMEGKSVLFKEFGNVDAFPICLNTQDTEEIISTVKAISPSFGGINLEDIAAPRCFEIEKRLIEELDIPVFH
DDQHGTAVIVLSAVINALRIVKKDISDVKIVISGAGAAGIAISNLLTLAGAKNNLILNSRGIIDPDDEKLDEARREIART
TNPNKVKGDLKTAMENADIFIGVSAPGILTKEMVSSMNHDAIVLAMANPVPEIYPDEAKEAGARVVGTGRSDYPNQVNNV
LVFPGIFRGALDAKADRITDDMKLSAAYAIAHMVEQPVEERILPEAFNKDVAKNVANAVKKSWEEKKVIK

Specific function: In addition to the NAD-dependent oxidative decarboxylation of L-malate, the enzyme catalyzes the decarboxylation of oxaloacetate [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=458, Percent_Identity=24.4541484716157, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI4505145, Length=423, Percent_Identity=23.4042553191489, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI62420882, Length=447, Percent_Identity=24.3847874720358, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI62420880, Length=447, Percent_Identity=24.3847874720358, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI239049447, Length=447, Percent_Identity=24.3847874720358, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI270265879, Length=369, Percent_Identity=23.3062330623306, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788806, Length=392, Percent_Identity=47.7040816326531, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI87081919, Length=449, Percent_Identity=26.9487750556793, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322823, Length=354, Percent_Identity=25.1412429378531, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281362674, Length=453, Percent_Identity=24.0618101545254, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281362672, Length=453, Percent_Identity=24.0618101545254, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21356279, Length=453, Percent_Identity=24.0618101545254, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI78707232, Length=361, Percent_Identity=24.6537396121884, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI281363503, Length=361, Percent_Identity=24.6537396121884, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI281363505, Length=361, Percent_Identity=24.6537396121884, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI78707236, Length=361, Percent_Identity=24.6537396121884, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI78707242, Length=365, Percent_Identity=26.5753424657534, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI78707238, Length=365, Percent_Identity=26.5753424657534, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI78707240, Length=365, Percent_Identity=26.5753424657534, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24646388, Length=404, Percent_Identity=25.4950495049505, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24646386, Length=404, Percent_Identity=25.4950495049505, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 42049; Mature: 42049

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEEIKQKALELHEKNQGKLEVVSKVKVNDAMDLALAYTPGVAEPCKKIHEDNSLAYKY
CCHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHEEEEECCCHHHHHHHHCCCCCEEEEE
TNKGNMVAVVTDGSAVLGLGNIGGIAGMPVMEGKSVLFKEFGNVDAFPICLNTQDTEEII
ECCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHH
STVKAISPSFGGINLEDIAAPRCFEIEKRLIEELDIPVFHDDQHGTAVIVLSAVINALRI
HHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHH
VKKDISDVKIVISGAGAAGIAISNLLTLAGAKNNLILNSRGIIDPDDEKLDEARREIART
HHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHC
TNPNKVKGDLKTAMENADIFIGVSAPGILTKEMVSSMNHDAIVLAMANPVPEIYPDEAKE
CCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHH
AGARVVGTGRSDYPNQVNNVLVFPGIFRGALDAKADRITDDMKLSAAYAIAHMVEQPVEE
CCCEEEECCCCCCCHHHCCEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
RILPEAFNKDVAKNVANAVKKSWEEKKVIK
HHCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNIEEIKQKALELHEKNQGKLEVVSKVKVNDAMDLALAYTPGVAEPCKKIHEDNSLAYKY
CCHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHEEEEECCCHHHHHHHHCCCCCEEEEE
TNKGNMVAVVTDGSAVLGLGNIGGIAGMPVMEGKSVLFKEFGNVDAFPICLNTQDTEEII
ECCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHH
STVKAISPSFGGINLEDIAAPRCFEIEKRLIEELDIPVFHDDQHGTAVIVLSAVINALRI
HHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHH
VKKDISDVKIVISGAGAAGIAISNLLTLAGAKNNLILNSRGIIDPDDEKLDEARREIART
HHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHC
TNPNKVKGDLKTAMENADIFIGVSAPGILTKEMVSSMNHDAIVLAMANPVPEIYPDEAKE
CCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHH
AGARVVGTGRSDYPNQVNNVLVFPGIFRGALDAKADRITDDMKLSAAYAIAHMVEQPVEE
CCCEEEECCCCCCCHHHCCEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
RILPEAFNKDVAKNVANAVKKSWEEKKVIK
HHCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644282 [H]