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The map label for this gene is tufA [H]

Identifier: 169824190

GI number: 169824190

Start: 555268

End: 556461

Strand: Direct

Name: tufA [H]

Synonym: FMG_0493

Alternate gene names: 169824190

Gene position: 555268-556461 (Clockwise)

Preceding gene: 169824189

Following gene: 169824191

Centisome position: 30.89

GC content: 40.87

Gene sequence:

>1194_bases
ATGAGTAAAGCTAAATTTGAAAGAACAAAACCACATGTTAACATTGGTACAATAGGTCACGTTGACCACGGTAAAACAAC
ATTAACTGCAGCAATCACACTTGTATTAAACAAGAGAATGGGATCAGGAGAATTCGTAGACTACGCTAACATCGACAAAG
CACCAGAAGAAAGAGAAAGAGGAATCACAATCAACACTTCTCACGTAGAATACGAAACAGAAAAAAGACACTACGCACAC
GTAGACTGTCCAGGCCACGCTGACTACGTTAAAAACATGATCACAGGAGCAGCACAAATGGACGGAGCAATCCTAGTAGT
AAGTGCAGCAGACGGTCCAATGCCACAAACAAGAGAACACATCTTGTTAGCAAGACAAGTAGGCGTACCAAAAATCGCAG
TATTCTTAAACAAAGAAGACCAAGTTGACGACCCAGAACTAATCGAATTAGTAGAAATGGAAGTTCGTGAACTATTAAAC
GAATATGAATACGAAGGAGACGACACACCAATCGTAGTAGGATCAGCATTAAAAGCATTAGAAGATCCAGACGGAGAATG
GGGAGACAAAATCATGAAATTGATGGAAGAAGTAGACGAATGGATCCCAGCTCCAGTAAGAGACGTTGACCACCCATTCC
TAATGCCAGTAGAAGACATCTTCACAATCACAGGACGTGGAACAGTAGCAACTGGTAGAGTAGAAAGAGGAAGAGTAAAA
GTTGGTGACAACGTAGAAATCGTAGGATTAACAGAAGAAAAAAGAACAGTAGTAGTAACAGGTGTAGAAATGTTCAGAAA
ACAACTTGACGAAGCAGAAGCAGGAGATAACATCGGAGCATTACTAAGAGGTGTACAAAGAGAAGAAATCGAAAGAGGAC
AAGTACTAGCAGCACCTGGAACAATTCACCCACACACAAAATTTGAAGCAGAAGTATACGTATTAACAAAAGACGAAGGT
GGAAGACACACACCATTCTTCTCAGGATACAGACCACAATTCTACTTCAGAACAACAGATGTAACAGGAAACATCGAACT
AGAAGAAGGCGTAGAAATGGTAATGCCAGGAGATAACGCAAAATTCATCATCGAATTGATCACTCCAATCGCAATCGAAG
AAGGACTAAGATTTGCCATCAGAGAAGGCGGTAGAACAGTAGGAGCAGGCGTTGTTTCTAAAATTATCGCATAG

Upstream 100 bases:

>100_bases
GCAACATATTCAATGCAATTCGATCATTATGAACAAGTTCCAAACTCAATATCAGAAGAAGTTATAGGAAAAAAGAATAA
TAAATAGGAGGCTTATATAA

Downstream 100 bases:

>100_bases
TTAAATACTAAGATTAATTAGCTCTCACTTAGGTGAGGGCTTTTTTGTTTGAAAAAAATATCTATAAAATATATAAAAAA
AAACCGACTAATATTTTAAT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu-A [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSKAKFERTKPHVNIGTIGHVDHGKTTLTAAITLVLNKRMGSGEFVDYANIDKAPEERERGITINTSHVEYETEKRHYAH
VDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPKIAVFLNKEDQVDDPELIELVEMEVRELLN
EYEYEGDDTPIVVGSALKALEDPDGEWGDKIMKLMEEVDEWIPAPVRDVDHPFLMPVEDIFTITGRGTVATGRVERGRVK
VGDNVEIVGLTEEKRTVVVTGVEMFRKQLDEAEAGDNIGALLRGVQREEIERGQVLAAPGTIHPHTKFEAEVYVLTKDEG
GRHTPFFSGYRPQFYFRTTDVTGNIELEEGVEMVMPGDNAKFIIELITPIAIEEGLRFAIREGGRTVGAGVVSKIIA

Sequences:

>Translated_397_residues
MSKAKFERTKPHVNIGTIGHVDHGKTTLTAAITLVLNKRMGSGEFVDYANIDKAPEERERGITINTSHVEYETEKRHYAH
VDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPKIAVFLNKEDQVDDPELIELVEMEVRELLN
EYEYEGDDTPIVVGSALKALEDPDGEWGDKIMKLMEEVDEWIPAPVRDVDHPFLMPVEDIFTITGRGTVATGRVERGRVK
VGDNVEIVGLTEEKRTVVVTGVEMFRKQLDEAEAGDNIGALLRGVQREEIERGQVLAAPGTIHPHTKFEAEVYVLTKDEG
GRHTPFFSGYRPQFYFRTTDVTGNIELEEGVEMVMPGDNAKFIIELITPIAIEEGLRFAIREGGRTVGAGVVSKIIA
>Mature_396_residues
SKAKFERTKPHVNIGTIGHVDHGKTTLTAAITLVLNKRMGSGEFVDYANIDKAPEERERGITINTSHVEYETEKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPKIAVFLNKEDQVDDPELIELVEMEVRELLNE
YEYEGDDTPIVVGSALKALEDPDGEWGDKIMKLMEEVDEWIPAPVRDVDHPFLMPVEDIFTITGRGTVATGRVERGRVKV
GDNVEIVGLTEEKRTVVVTGVEMFRKQLDEAEAGDNIGALLRGVQREEIERGQVLAAPGTIHPHTKFEAEVYVLTKDEGG
RHTPFFSGYRPQFYFRTTDVTGNIELEEGVEMVMPGDNAKFIIELITPIAIEEGLRFAIREGGRTVGAGVVSKIIA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=397, Percent_Identity=57.1788413098237, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI223555963, Length=446, Percent_Identity=30.0448430493274, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI5729864, Length=446, Percent_Identity=30.0448430493274, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI4503471, Length=450, Percent_Identity=31.7777777777778, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI4503475, Length=451, Percent_Identity=31.9290465631929, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI54607086, Length=321, Percent_Identity=29.9065420560748, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI194018522, Length=443, Percent_Identity=27.313769751693, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI194097354, Length=443, Percent_Identity=27.313769751693, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI194018520, Length=443, Percent_Identity=27.313769751693, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI46094014, Length=441, Percent_Identity=26.530612244898, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI157426893, Length=284, Percent_Identity=27.112676056338, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1790412, Length=397, Percent_Identity=74.5591939546599, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI1789737, Length=397, Percent_Identity=74.5591939546599, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI2367247, Length=382, Percent_Identity=30.8900523560209, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI48994988, Length=276, Percent_Identity=33.695652173913, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1789108, Length=328, Percent_Identity=30.1829268292683, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789738, Length=146, Percent_Identity=34.2465753424658, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788922, Length=142, Percent_Identity=33.8028169014084, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=52.25, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=388, Percent_Identity=42.5257731958763, Blast_Score=317, Evalue=8e-87,
Organism=Caenorhabditis elegans, GI17552884, Length=453, Percent_Identity=32.0088300220751, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17569207, Length=453, Percent_Identity=32.0088300220751, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI32566303, Length=426, Percent_Identity=31.4553990610329, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI115532067, Length=429, Percent_Identity=28.4382284382284, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=426, Percent_Identity=28.4037558685446, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI32566629, Length=451, Percent_Identity=27.2727272727273, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=301, Percent_Identity=28.9036544850498, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=144, Percent_Identity=41.6666666666667, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17509919, Length=443, Percent_Identity=26.6365688487585, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=234, Percent_Identity=27.7777777777778, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71994658, Length=249, Percent_Identity=31.3253012048193, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=64.321608040201, Blast_Score=524, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6325337, Length=447, Percent_Identity=31.0961968680089, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=447, Percent_Identity=31.0961968680089, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=450, Percent_Identity=25.3333333333333, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6320377, Length=348, Percent_Identity=29.0229885057471, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=282, Percent_Identity=26.5957446808511, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=265, Percent_Identity=29.811320754717, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=142, Percent_Identity=34.5070422535211, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281363316, Length=395, Percent_Identity=57.4683544303797, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI17864358, Length=395, Percent_Identity=57.4683544303797, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI19921738, Length=388, Percent_Identity=47.4226804123711, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45553807, Length=447, Percent_Identity=30.6487695749441, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI45553816, Length=447, Percent_Identity=30.6487695749441, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24651721, Length=447, Percent_Identity=30.6487695749441, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI17864154, Length=447, Percent_Identity=30.6487695749441, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24652838, Length=445, Percent_Identity=31.0112359550562, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI17137572, Length=445, Percent_Identity=31.0112359550562, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI45550900, Length=439, Percent_Identity=29.6127562642369, Blast_Score=141, Evalue=8e-34,
Organism=Drosophila melanogaster, GI19922690, Length=306, Percent_Identity=31.3725490196078, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI17137380, Length=344, Percent_Identity=30.2325581395349, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24647048, Length=430, Percent_Identity=27.2093023255814, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24647046, Length=430, Percent_Identity=27.2093023255814, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28572034, Length=167, Percent_Identity=36.5269461077844, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43953; Mature: 43821

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAKFERTKPHVNIGTIGHVDHGKTTLTAAITLVLNKRMGSGEFVDYANIDKAPEERER
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHEEHHHCCCCCCCEEECCCCCCCCHHHHC
GITINTSHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREH
CEEEECCEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHH
ILLARQVGVPKIAVFLNKEDQVDDPELIELVEMEVRELLNEYEYEGDDTPIVVGSALKAL
HHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHH
EDPDGEWGDKIMKLMEEVDEWIPAPVRDVDHPFLMPVEDIFTITGRGTVATGRVERGRVK
CCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHEEEECCCEEEECCCCCCEEE
VGDNVEIVGLTEEKRTVVVTGVEMFRKQLDEAEAGDNIGALLRGVQREEIERGQVLAAPG
ECCCEEEEEECCCCCEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEECCC
TIHPHTKFEAEVYVLTKDEGGRHTPFFSGYRPQFYFRTTDVTGNIELEEGVEMVMPGDNA
CCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEEHHCCCEEEECCCCC
KFIIELITPIAIEEGLRFAIREGGRTVGAGVVSKIIA
EEEEEHHHHHHHHHCHHHHHHCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
SKAKFERTKPHVNIGTIGHVDHGKTTLTAAITLVLNKRMGSGEFVDYANIDKAPEERER
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHEEHHHCCCCCCCEEECCCCCCCCHHHHC
GITINTSHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREH
CEEEECCEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHH
ILLARQVGVPKIAVFLNKEDQVDDPELIELVEMEVRELLNEYEYEGDDTPIVVGSALKAL
HHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHH
EDPDGEWGDKIMKLMEEVDEWIPAPVRDVDHPFLMPVEDIFTITGRGTVATGRVERGRVK
CCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHEEEECCCEEEECCCCCCEEE
VGDNVEIVGLTEEKRTVVVTGVEMFRKQLDEAEAGDNIGALLRGVQREEIERGQVLAAPG
ECCCEEEEEECCCCCEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEECCC
TIHPHTKFEAEVYVLTKDEGGRHTPFFSGYRPQFYFRTTDVTGNIELEEGVEMVMPGDNA
CCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEEHHCCCEEEECCCCC
KFIIELITPIAIEEGLRFAIREGGRTVGAGVVSKIIA
EEEEEHHHHHHHHHCHHHHHHCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]