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The map label for this gene is yiaE [C]

Identifier: 169824104

GI number: 169824104

Start: 462512

End: 463453

Strand: Direct

Name: yiaE [C]

Synonym: FMG_0407

Alternate gene names: 169824104

Gene position: 462512-463453 (Clockwise)

Preceding gene: 169824103

Following gene: 169824105

Centisome position: 25.73

GC content: 32.06

Gene sequence:

>942_bases
ATGAAAGTTTTGATTACAAATAGAATTCCAAACGAAATTATAAAAAAATACAAAAAAAATTTTGAAGTAGACTACAATGA
TAGTTTGGAATTTTTGTCAAAAGAAGAATTGAAAACTAGAATAAAAGATTCAGAAGCATTGGTGTGTCCTTTGAGCGAAA
AAATCGATTCAGAAATTATTGATGCGGCGAATAATTTGAAAATAATCGCAAATTACGGAGCTGGATTTGATAATGTGGAT
ATTAATTACGCAAAAGAAAAAGGAATAATTGTCACAAATGCGCCCGCATCTGCATCTACAAAATCAACTGCAGAATTAAC
TTTTGGTTTAATTATAGATTTGCTTAGAAATATCACGAAGATGAATTCTGATTGTTATGATGACTCTTTTGAAGGGTGGA
AACCTGTGTATGGTTTGGGAGAAACTTTGCAAGGAAAGACTTTGGGAATAATTGGACTCGGAAGAATTGGAACTGAAGTT
ATGAGAAAAGCAAAAGCATTTGACATGGATGTTATTTTCTACAATCGCAGCAAAAAAGAAGTTGAAGGGGCAAAGCAAGT
AGATTTAGATTTTCTTTTAGAAAATAGTGACATTATAACTTTACACACTGCTTATAGCGACGAATTACATCATTTAATAG
ATGCAAAAGCATTATCAAAAATGAAAAAAACCGCTTTTTTAATCAACGCATCTCGTGGCAAAGTTATTAGCGAAGAAGAT
TTGATAGATGCATTAAACGATGGTGAAATTTCTGGTTGTGCGTTGGATGTGTACGAATTTGAACCAAAAATTTCAGATGA
ATTGAGAAATGCCAAGAATATTTTGTTGGCACCTCATTTGGGAAATGCGACTAAACTTGCACGCGTTCAAATGGGAGAGT
TTACTTTTGACAATATTATGCAATTTAAAAACGGAGAAATCCCTAAAAATAAAGTAAATTAG

Upstream 100 bases:

>100_bases
ACTTTGATGATGCATTTAAGCTATTAAAAAGCGTAGATGCAAAGTATTTGGCGATGTTTGAAAATAGAAAAATGACTACA
ATAAAATTGGAGGACATGTA

Downstream 100 bases:

>100_bases
TTTAATATCGCGTTTAATTATGGTATACTAATTGAGTAAAAAAATGAAAAGGGGAATTTATGACTGATGATATAAAAGCA
TTGTTCCAATCAAAAGGATT

Product: putative dehydrogenase

Products: glycerate; NADP; NADPH; glyoxylate; 2-Dehydro-D-gluconate; 5-ketogluconate; L-idonate [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKVLITNRIPNEIIKKYKKNFEVDYNDSLEFLSKEELKTRIKDSEALVCPLSEKIDSEIIDAANNLKIIANYGAGFDNVD
INYAKEKGIIVTNAPASASTKSTAELTFGLIIDLLRNITKMNSDCYDDSFEGWKPVYGLGETLQGKTLGIIGLGRIGTEV
MRKAKAFDMDVIFYNRSKKEVEGAKQVDLDFLLENSDIITLHTAYSDELHHLIDAKALSKMKKTAFLINASRGKVISEED
LIDALNDGEISGCALDVYEFEPKISDELRNAKNILLAPHLGNATKLARVQMGEFTFDNIMQFKNGEIPKNKVN

Sequences:

>Translated_313_residues
MKVLITNRIPNEIIKKYKKNFEVDYNDSLEFLSKEELKTRIKDSEALVCPLSEKIDSEIIDAANNLKIIANYGAGFDNVD
INYAKEKGIIVTNAPASASTKSTAELTFGLIIDLLRNITKMNSDCYDDSFEGWKPVYGLGETLQGKTLGIIGLGRIGTEV
MRKAKAFDMDVIFYNRSKKEVEGAKQVDLDFLLENSDIITLHTAYSDELHHLIDAKALSKMKKTAFLINASRGKVISEED
LIDALNDGEISGCALDVYEFEPKISDELRNAKNILLAPHLGNATKLARVQMGEFTFDNIMQFKNGEIPKNKVN
>Mature_313_residues
MKVLITNRIPNEIIKKYKKNFEVDYNDSLEFLSKEELKTRIKDSEALVCPLSEKIDSEIIDAANNLKIIANYGAGFDNVD
INYAKEKGIIVTNAPASASTKSTAELTFGLIIDLLRNITKMNSDCYDDSFEGWKPVYGLGETLQGKTLGIIGLGRIGTEV
MRKAKAFDMDVIFYNRSKKEVEGAKQVDLDFLLENSDIITLHTAYSDELHHLIDAKALSKMKKTAFLINASRGKVISEED
LIDALNDGEISGCALDVYEFEPKISDELRNAKNILLAPHLGNATKLARVQMGEFTFDNIMQFKNGEIPKNKVN

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=266, Percent_Identity=36.8421052631579, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI6912396, Length=315, Percent_Identity=33.015873015873, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI61743967, Length=262, Percent_Identity=37.7862595419847, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI4557497, Length=262, Percent_Identity=37.7862595419847, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI145580578, Length=262, Percent_Identity=37.0229007633588, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI4557499, Length=262, Percent_Identity=37.0229007633588, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI145580575, Length=267, Percent_Identity=35.9550561797753, Blast_Score=145, Evalue=4e-35,
Organism=Escherichia coli, GI87082289, Length=320, Percent_Identity=29.6875, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1789279, Length=268, Percent_Identity=34.7014925373134, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787645, Length=326, Percent_Identity=29.7546012269939, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI87081824, Length=165, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788660, Length=274, Percent_Identity=25.9124087591241, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=312, Percent_Identity=32.6923076923077, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25147481, Length=270, Percent_Identity=31.4814814814815, Blast_Score=121, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6322116, Length=308, Percent_Identity=34.4155844155844, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320925, Length=308, Percent_Identity=33.7662337662338, Blast_Score=155, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6324055, Length=236, Percent_Identity=34.7457627118644, Blast_Score=146, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6324964, Length=268, Percent_Identity=26.4925373134328, Blast_Score=86, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6321253, Length=178, Percent_Identity=28.6516853932584, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325144, Length=293, Percent_Identity=24.9146757679181, Blast_Score=69, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324980, Length=161, Percent_Identity=26.7080745341615, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI28574284, Length=315, Percent_Identity=31.7460317460317, Blast_Score=154, Evalue=5e-38,
Organism=Drosophila melanogaster, GI45551003, Length=315, Percent_Identity=31.7460317460317, Blast_Score=154, Evalue=5e-38,
Organism=Drosophila melanogaster, GI45552429, Length=315, Percent_Identity=31.7460317460317, Blast_Score=154, Evalue=6e-38,
Organism=Drosophila melanogaster, GI24585514, Length=315, Percent_Identity=31.7460317460317, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI28574282, Length=315, Percent_Identity=31.7460317460317, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI28574286, Length=318, Percent_Identity=31.7610062893082, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI28571528, Length=296, Percent_Identity=31.7567567567568, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI19921140, Length=317, Percent_Identity=30.5993690851735, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24646446, Length=263, Percent_Identity=33.8403041825095, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24646448, Length=263, Percent_Identity=33.8403041825095, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24646452, Length=263, Percent_Identity=33.8403041825095, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24646450, Length=263, Percent_Identity=33.8403041825095, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI62472511, Length=263, Percent_Identity=33.8403041825095, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24585516, Length=280, Percent_Identity=25.7142857142857, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: NA

Molecular weight: Translated: 34954; Mature: 34954

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLITNRIPNEIIKKYKKNFEVDYNDSLEFLSKEELKTRIKDSEALVCPLSEKIDSEII
CEEEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHH
DAANNLKIIANYGAGFDNVDINYAKEKGIIVTNAPASASTKSTAELTFGLIIDLLRNITK
CCCCCEEEEEECCCCCCCCCEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
MNSDCYDDSFEGWKPVYGLGETLQGKTLGIIGLGRIGTEVMRKAKAFDMDVIFYNRSKKE
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHCCEEEEEECCCHHH
VEGAKQVDLDFLLENSDIITLHTAYSDELHHLIDAKALSKMKKTAFLINASRGKVISEED
HCCCHHCCEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHEEEEECCCCCEECHHH
LIDALNDGEISGCALDVYEFEPKISDELRNAKNILLAPHLGNATKLARVQMGEFTFDNIM
HHHHCCCCCCCCEEEEEEECCCCHHHHHHCCCCEEEECCCCCCHHEEEEEECCCCHHHHH
QFKNGEIPKNKVN
HHCCCCCCCCCCC
>Mature Secondary Structure
MKVLITNRIPNEIIKKYKKNFEVDYNDSLEFLSKEELKTRIKDSEALVCPLSEKIDSEII
CEEEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHH
DAANNLKIIANYGAGFDNVDINYAKEKGIIVTNAPASASTKSTAELTFGLIIDLLRNITK
CCCCCEEEEEECCCCCCCCCEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
MNSDCYDDSFEGWKPVYGLGETLQGKTLGIIGLGRIGTEVMRKAKAFDMDVIFYNRSKKE
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHCCEEEEEECCCHHH
VEGAKQVDLDFLLENSDIITLHTAYSDELHHLIDAKALSKMKKTAFLINASRGKVISEED
HCCCHHCCEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHEEEEECCCCCEECHHH
LIDALNDGEISGCALDVYEFEPKISDELRNAKNILLAPHLGNATKLARVQMGEFTFDNIM
HHHHCCCCCCCCEEEEEEECCCCHHHHHHCCCCEEEECCCCCCHHEEEEEECCCCHHHHH
QFKNGEIPKNKVN
HHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: hydroxypyruvate; NADPH; glycolate; NADP; gluconate; 2,5-diketo-D-gluconate; 2-keto-L-gulonate [C]

Specific reaction: hydroxypyruvate + NADPH = glycerate + NADP glycolate + NADP = NADPH + glyoxylate NADP + gluconate = NADPH + 2-Dehydro-D-gluconate 2,5-diketo-D-gluconate + NADPH = 5-ketogluconate + NADP 2-keto-L-gulonate + NADPH = L-idonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12950922 [H]