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The map label for this gene is metE [H]

Identifier: 169824085

GI number: 169824085

Start: 445893

End: 447068

Strand: Direct

Name: metE [H]

Synonym: FMG_0388

Alternate gene names: 169824085

Gene position: 445893-447068 (Clockwise)

Preceding gene: 169824084

Following gene: 169824086

Centisome position: 24.81

GC content: 34.35

Gene sequence:

>1176_bases
GTGAACAATAAATTATTCAAGACCTCTTTGATAGGATCTATGCCAAGAGGAAATGAAATATTGAAAGCTCGTAGGATGTT
AAAGGCTAATATGATTAGCCTTTCTGACTACGAACAAATGGTATATGATAAAACTAAAGAAGTTGTAAAATTACAAGAAG
ATTTGGACATTGATGTAATTACAAGTGGAGAAATTGAAAGAGACAACTATGTTTCTTTTATCTCTGAAAAGCTTGGCGGC
GTAACTCAAATGAGCATGGCAGAAATGCTAGATTATGTCGATGATAAAAGAGAATTCGAAAATATTTTGACAACGTTGGA
TGTACCTGCTACATCTATTAAAAATGCTATTTGTACTGGCAAATTGGAATACAAAGGAGACATTGTATCAGAAGAATTAA
AGCTTTTGAAATCTTTGACTGATAAGCCTGTTAAGATTACAATGCCAGGCCCTTACTTGGTTACAAGAAGTATGTGGCTT
GCAAATGTAAGCTCAAAATACTACGATAGTAAGGAAGCTTTGGGTGAAGATGTAATAGACATTTACAAGAAAGAAATCAA
AAATCTTCAAGAAATCGGAGTAGATGTAATCCAATTCGACGAACCAGTTCTTACAGAAATTGTGTTCACTGAAGGTAAAC
CAAGAACATTCATGTGTGCATCATTGTCTACTAGAAAAGATCCAACTGAAGAGTTAAAGTTTGCCACACATTTGATAAAA
AGCGTGATGGATAGTGTCGATAGGGACAAATCAATATGTTCACTTCATGTGTGTCGTGGAAACTGGAGTAAAAACGAAAG
TATTTTATTGACAGGACCTTATACTCCACTTTTGGATTTGTTTGCAGATATCAATGCTGATTTATTAGCGTTAGAATTTT
CAACTCCAAGAGCTGGAGAATTGAAAGCATTACTAGCTGATGAAAGAATCAACAACAAAGTGATTTTGGGACTTGGAGTG
TTAAATCCAAGATTAGATGACAAGGAAGATACAGATGGAATTTACAAAAGAGCCAAGGAAGCTCTTACATTTATCGACAA
GGATAAATTGTGGCTAAATCCAGACTGTGGATTTGCTACATTCTCCAATAGACCAGTAAATGAATACGACCACATAAGAG
AAAAATTACAATCGATGATTGATGCTAGAAATAAATTGAGGAAAGAATATGAATGA

Upstream 100 bases:

>100_bases
CTTGAAGGTTTGAAACAATGGGTTCCTGGAAGAAGAGATGGTTTCAAACAAATTACAGAAGCTAATGAATATTTAGGAAA
TTTCATAAAGGAATACAATA

Downstream 100 bases:

>100_bases
TGATATAAATTCATTCCAAAAAAATTTCAGAGCTATTGTATCCGATGATTTGGTTAAAGTTTACAACGAAAAATCATGTA
TTCCAGTGGACTCATCTATT

Product: 5-methyltetrahydropteroyltriglutamate- homocysteine methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MNNKLFKTSLIGSMPRGNEILKARRMLKANMISLSDYEQMVYDKTKEVVKLQEDLDIDVITSGEIERDNYVSFISEKLGG
VTQMSMAEMLDYVDDKREFENILTTLDVPATSIKNAICTGKLEYKGDIVSEELKLLKSLTDKPVKITMPGPYLVTRSMWL
ANVSSKYYDSKEALGEDVIDIYKKEIKNLQEIGVDVIQFDEPVLTEIVFTEGKPRTFMCASLSTRKDPTEELKFATHLIK
SVMDSVDRDKSICSLHVCRGNWSKNESILLTGPYTPLLDLFADINADLLALEFSTPRAGELKALLADERINNKVILGLGV
LNPRLDDKEDTDGIYKRAKEALTFIDKDKLWLNPDCGFATFSNRPVNEYDHIREKLQSMIDARNKLRKEYE

Sequences:

>Translated_391_residues
MNNKLFKTSLIGSMPRGNEILKARRMLKANMISLSDYEQMVYDKTKEVVKLQEDLDIDVITSGEIERDNYVSFISEKLGG
VTQMSMAEMLDYVDDKREFENILTTLDVPATSIKNAICTGKLEYKGDIVSEELKLLKSLTDKPVKITMPGPYLVTRSMWL
ANVSSKYYDSKEALGEDVIDIYKKEIKNLQEIGVDVIQFDEPVLTEIVFTEGKPRTFMCASLSTRKDPTEELKFATHLIK
SVMDSVDRDKSICSLHVCRGNWSKNESILLTGPYTPLLDLFADINADLLALEFSTPRAGELKALLADERINNKVILGLGV
LNPRLDDKEDTDGIYKRAKEALTFIDKDKLWLNPDCGFATFSNRPVNEYDHIREKLQSMIDARNKLRKEYE
>Mature_391_residues
MNNKLFKTSLIGSMPRGNEILKARRMLKANMISLSDYEQMVYDKTKEVVKLQEDLDIDVITSGEIERDNYVSFISEKLGG
VTQMSMAEMLDYVDDKREFENILTTLDVPATSIKNAICTGKLEYKGDIVSEELKLLKSLTDKPVKITMPGPYLVTRSMWL
ANVSSKYYDSKEALGEDVIDIYKKEIKNLQEIGVDVIQFDEPVLTEIVFTEGKPRTFMCASLSTRKDPTEELKFATHLIK
SVMDSVDRDKSICSLHVCRGNWSKNESILLTGPYTPLLDLFADINADLLALEFSTPRAGELKALLADERINNKVILGLGV
LNPRLDDKEDTDGIYKRAKEALTFIDKDKLWLNPDCGFATFSNRPVNEYDHIREKLQSMIDARNKLRKEYE

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=384, Percent_Identity=25, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6320936, Length=394, Percent_Identity=23.8578680203046, Blast_Score=99, Evalue=1e-21,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 44500; Mature: 44500

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKLFKTSLIGSMPRGNEILKARRMLKANMISLSDYEQMVYDKTKEVVKLQEDLDIDVI
CCCCEEHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEE
TSGEIERDNYVSFISEKLGGVTQMSMAEMLDYVDDKREFENILTTLDVPATSIKNAICTG
ECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
KLEYKGDIVSEELKLLKSLTDKPVKITMPGPYLVTRSMWLANVSSKYYDSKEALGEDVID
CEECCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
IYKKEIKNLQEIGVDVIQFDEPVLTEIVFTEGKPRTFMCASLSTRKDPTEELKFATHLIK
HHHHHHHHHHHCCCEEEECCCHHHHHHEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHH
SVMDSVDRDKSICSLHVCRGNWSKNESILLTGPYTPLLDLFADINADLLALEFSTPRAGE
HHHHHHCCCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCCH
LKALLADERINNKVILGLGVLNPRLDDKEDTDGIYKRAKEALTFIDKDKLWLNPDCGFAT
HHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCC
FSNRPVNEYDHIREKLQSMIDARNKLRKEYE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNKLFKTSLIGSMPRGNEILKARRMLKANMISLSDYEQMVYDKTKEVVKLQEDLDIDVI
CCCCEEHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEE
TSGEIERDNYVSFISEKLGGVTQMSMAEMLDYVDDKREFENILTTLDVPATSIKNAICTG
ECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
KLEYKGDIVSEELKLLKSLTDKPVKITMPGPYLVTRSMWLANVSSKYYDSKEALGEDVID
CEECCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
IYKKEIKNLQEIGVDVIQFDEPVLTEIVFTEGKPRTFMCASLSTRKDPTEELKFATHLIK
HHHHHHHHHHHCCCEEEECCCHHHHHHEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHH
SVMDSVDRDKSICSLHVCRGNWSKNESILLTGPYTPLLDLFADINADLLALEFSTPRAGE
HHHHHHCCCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCCH
LKALLADERINNKVILGLGVLNPRLDDKEDTDGIYKRAKEALTFIDKDKLWLNPDCGFAT
HHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCC
FSNRPVNEYDHIREKLQSMIDARNKLRKEYE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA