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The map label for this gene is fadE25 [H]

Identifier: 169824083

GI number: 169824083

Start: 443804

End: 444931

Strand: Direct

Name: fadE25 [H]

Synonym: FMG_0386

Alternate gene names: 169824083

Gene position: 443804-444931 (Clockwise)

Preceding gene: 169824082

Following gene: 169824084

Centisome position: 24.69

GC content: 35.28

Gene sequence:

>1128_bases
ATGAATTTTTACGAAGAATCAAGAAAATTTGCATTGGAACATGTAGAACCATATGCAAAGAGATCAGATGAAGAAGGTAG
ATTTCCTGTAGAATCATTTGAAGAAATGGGAAAAGCTGGCTATTTCAAATTACTTATTCCAGAAGAAATGGGTGGTTTAG
GAAAAACTGCTGTTGAACATCAACAAGCTGTTTTAGCATTTGCTGAAAGCAACCCAACTGCTGGCCTTTGTTACATGATG
CACAACGTAGCATTGATGACAGTACTTACTAATGGCAACGAAGAATTAAAGAAAAAAGTTGTAAAAGACATAGTTGAAAA
CAACAAATTCATGGCTTTAGCATATTCTGAATTTGGAACTGGTACTCACTTCTACATTCCAGAAATCAAAGTTACTAAAG
ATGGTGGAAAATTTGTTTTCAATGGAACAAAATCAATGGTTACAAGTGCAACTCATGCAAGCTATTACTTAATTTTGACT
CCATCAACAGAAAAAGAAGGAGACATCAACAACTGGTTAATTCCATTAGAATCTGAAGGATTAGAATTCAAGATGAGTCA
TTGGAAAGGTATTGGTATGAAAGGTAATGTTTCATGTCCAATGACTATGACAGATGTTAAATTAGACGAAGTTAACAGAA
TCGGTGAAGAAGGTTCAGGTGTTGATCAAGTGTTCAACGTTGTAGCTCCATACTTCATCTTAGGTTTAGCAAGTGTATAC
ACTGGATTAAACTTAAGATTATCAAAAATTACAAATGACTATGCATTAAAGAGAAAATATCCATCAGGACAATGTTTAGC
TAATATTGAAACAGTACAAGTTCACTTAGCTAAGATTTATGCTAACGGAATGAGTGCAAAAGCTCTTACAGAATTAGCAG
CAAGATCATTAGTTGATGGTGAAGCTGATGCAGTATGCAAGATTATCGCAGCAAGAGTTTGTGCTATCGAAAATGCTATT
GAATCTTCAACACTTGCAATGAGAGTTGGCGGCGGAAAGACTTACAACTGTGCATCTGAAATCCAAAGATTAATGAGAGA
TGCTTACGCTGGACAAATTATGGCTCCATCACTAGACGTTCTTAACGTTTGGTTAGGAAAAGCTATCACAGGTCAACCAC
TATTATAA

Upstream 100 bases:

>100_bases
GAAAGTTTAAATAAATGGGCAAAATTTAACAATTTGTGTAGTTTTCTTACAGAATAAGTGATAATATAAATATGAACTAT
ATAAACTTTAGGAGGGTTTT

Downstream 100 bases:

>100_bases
TATTGTAAATTTATCGGAATTTATGAACAGTAAAGTTTTTGCTTACAAACAAATATTCTAACTGTTTGTAAGTTCCGATT
TTTATTTGGATTTTCTAGAA

Product: putative acyl-CoA dehydrogenase

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MNFYEESRKFALEHVEPYAKRSDEEGRFPVESFEEMGKAGYFKLLIPEEMGGLGKTAVEHQQAVLAFAESNPTAGLCYMM
HNVALMTVLTNGNEELKKKVVKDIVENNKFMALAYSEFGTGTHFYIPEIKVTKDGGKFVFNGTKSMVTSATHASYYLILT
PSTEKEGDINNWLIPLESEGLEFKMSHWKGIGMKGNVSCPMTMTDVKLDEVNRIGEEGSGVDQVFNVVAPYFILGLASVY
TGLNLRLSKITNDYALKRKYPSGQCLANIETVQVHLAKIYANGMSAKALTELAARSLVDGEADAVCKIIAARVCAIENAI
ESSTLAMRVGGGKTYNCASEIQRLMRDAYAGQIMAPSLDVLNVWLGKAITGQPLL

Sequences:

>Translated_375_residues
MNFYEESRKFALEHVEPYAKRSDEEGRFPVESFEEMGKAGYFKLLIPEEMGGLGKTAVEHQQAVLAFAESNPTAGLCYMM
HNVALMTVLTNGNEELKKKVVKDIVENNKFMALAYSEFGTGTHFYIPEIKVTKDGGKFVFNGTKSMVTSATHASYYLILT
PSTEKEGDINNWLIPLESEGLEFKMSHWKGIGMKGNVSCPMTMTDVKLDEVNRIGEEGSGVDQVFNVVAPYFILGLASVY
TGLNLRLSKITNDYALKRKYPSGQCLANIETVQVHLAKIYANGMSAKALTELAARSLVDGEADAVCKIIAARVCAIENAI
ESSTLAMRVGGGKTYNCASEIQRLMRDAYAGQIMAPSLDVLNVWLGKAITGQPLL
>Mature_375_residues
MNFYEESRKFALEHVEPYAKRSDEEGRFPVESFEEMGKAGYFKLLIPEEMGGLGKTAVEHQQAVLAFAESNPTAGLCYMM
HNVALMTVLTNGNEELKKKVVKDIVENNKFMALAYSEFGTGTHFYIPEIKVTKDGGKFVFNGTKSMVTSATHASYYLILT
PSTEKEGDINNWLIPLESEGLEFKMSHWKGIGMKGNVSCPMTMTDVKLDEVNRIGEEGSGVDQVFNVVAPYFILGLASVY
TGLNLRLSKITNDYALKRKYPSGQCLANIETVQVHLAKIYANGMSAKALTELAARSLVDGEADAVCKIIAARVCAIENAI
ESSTLAMRVGGGKTYNCASEIQRLMRDAYAGQIMAPSLDVLNVWLGKAITGQPLL

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557233, Length=354, Percent_Identity=25.7062146892655, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI4501859, Length=349, Percent_Identity=23.7822349570201, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI226958412, Length=372, Percent_Identity=23.6559139784946, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI7656849, Length=373, Percent_Identity=22.7882037533512, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI21361497, Length=373, Percent_Identity=23.8605898123324, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI187960098, Length=359, Percent_Identity=23.9554317548747, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI4557231, Length=359, Percent_Identity=23.9554317548747, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI226958414, Length=348, Percent_Identity=22.9885057471264, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI4501857, Length=295, Percent_Identity=24.7457627118644, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1786223, Length=372, Percent_Identity=23.6559139784946, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87081958, Length=360, Percent_Identity=22.7777777777778, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17569725, Length=372, Percent_Identity=26.3440860215054, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17570075, Length=359, Percent_Identity=25.3481894150418, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17538396, Length=373, Percent_Identity=26.5415549597855, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17534899, Length=369, Percent_Identity=23.5772357723577, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17508101, Length=353, Percent_Identity=22.6628895184136, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17533517, Length=372, Percent_Identity=21.2365591397849, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17506239, Length=359, Percent_Identity=22.0055710306407, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71985184, Length=359, Percent_Identity=21.7270194986072, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71982178, Length=219, Percent_Identity=23.7442922374429, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356377, Length=349, Percent_Identity=26.3610315186246, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI21355753, Length=374, Percent_Identity=22.7272727272727, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24660351, Length=365, Percent_Identity=24.3835616438356, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24646207, Length=364, Percent_Identity=22.5274725274725, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24666513, Length=297, Percent_Identity=24.2424242424242, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281363737, Length=362, Percent_Identity=21.2707182320442, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 41166; Mature: 41166

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFYEESRKFALEHVEPYAKRSDEEGRFPVESFEEMGKAGYFKLLIPEEMGGLGKTAVEH
CCCHHHHHHHHHHHHCHHHHCCCCCCCCCHHHHHHCCCCCEEEEEECHHCCCCCHHHHHH
QQAVLAFAESNPTAGLCYMMHNVALMTVLTNGNEELKKKVVKDIVENNKFMALAYSEFGT
HHHEEEEECCCCCCHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCC
GTHFYIPEIKVTKDGGKFVFNGTKSMVTSATHASYYLILTPSTEKEGDINNWLIPLESEG
CCEEEECEEEEECCCCEEEECCCHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEEECCCC
LEFKMSHWKGIGMKGNVSCPMTMTDVKLDEVNRIGEEGSGVDQVFNVVAPYFILGLASVY
CEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
TGLNLRLSKITNDYALKRKYPSGQCLANIETVQVHLAKIYANGMSAKALTELAARSLVDG
CCCCEEEEHHCCCHHHEECCCCCCEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
EADAVCKIIAARVCAIENAIESSTLAMRVGGGKTYNCASEIQRLMRDAYAGQIMAPSLDV
CHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEECCCHHH
LNVWLGKAITGQPLL
HHHHHCCCCCCCCCC
>Mature Secondary Structure
MNFYEESRKFALEHVEPYAKRSDEEGRFPVESFEEMGKAGYFKLLIPEEMGGLGKTAVEH
CCCHHHHHHHHHHHHCHHHHCCCCCCCCCHHHHHHCCCCCEEEEEECHHCCCCCHHHHHH
QQAVLAFAESNPTAGLCYMMHNVALMTVLTNGNEELKKKVVKDIVENNKFMALAYSEFGT
HHHEEEEECCCCCCHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCC
GTHFYIPEIKVTKDGGKFVFNGTKSMVTSATHASYYLILTPSTEKEGDINNWLIPLESEG
CCEEEECEEEEECCCCEEEECCCHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEEECCCC
LEFKMSHWKGIGMKGNVSCPMTMTDVKLDEVNRIGEEGSGVDQVFNVVAPYFILGLASVY
CEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
TGLNLRLSKITNDYALKRKYPSGQCLANIETVQVHLAKIYANGMSAKALTELAARSLVDG
CCCCEEEEHHCCCHHHEECCCCCCEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
EADAVCKIIAARVCAIENAIESSTLAMRVGGGKTYNCASEIQRLMRDAYAGQIMAPSLDV
CHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEECCCHHH
LNVWLGKAITGQPLL
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]