Click here to switch to the map view.

The map label for this gene is topB [H]

Identifier: 169823682

GI number: 169823682

Start: 165519

End: 167537

Strand: Reverse

Name: topB [H]

Synonym: FMG_P0168

Alternate gene names: 169823682

Gene position: 167537-165519 (Counterclockwise)

Preceding gene: 169823683

Following gene: 169823681

Centisome position: 88.57

GC content: 28.73

Gene sequence:

>2019_bases
TTGAAATTAATAATAGCAGAAAAAAAAGACATGGCACAGAAAATAGCGGATTCTATTGGTAAATTTACACCGGAAGATGG
CTATTATGATGTTTTAGATTGTCATGTAACATGGACTTGGGGTCATTTATTTGGCTTAAAAGAGCCGAATGAATACGAAA
ATCAAGGATGGGATAAAAAATGGGCTGATAGTAGTATAGATGATTTACCTATGATTCCAAATAAATACCAATATAAACTA
ACAGGTAATAAAGATCAATTTAAAAAAATTGAAGATTTAATAAAAAGTAATAAATACGATGAAATAATTAACTCTTGCGA
TGCTGAAAGAGAAGGAGAGTTAATATTTAGACTTCTATATAATCACTTAGATATAGACGAGAACAAAACAAAGGTTACTC
GTATGTGGTGTAATACAACGAATAAAGACGGACTTTTAAAAGCTTTAGAGGAAAGAAAAGACATAAAAGAGTATGACGGA
TATTACCTAGAGGCAGATTCAAGAAGTAAGGCAGACTGGCTAATAGGTATGAACTTATCAAGATTGTATATGTTTAAATT
TTTCAGAAAGTTTGTAATAGGTCGTGTAATGACACCTACTTTATGTTTAATCATAGAAAGAGATTTAGAGATAGAAAACT
TCGAAGAAAGTGATATATATAACCTCGTTGGAGAGTATAACGGATTTCAATTTAAAAGAAAGTTCGATACTCCTGAAGAG
TGTGATAGATTTAAAGATTTAAAAGACTTTACAGTAGTAGATATAGTAGAAGAAAAGAAAAAGAAAAAAGCACCTAAATT
ATTTAAATTATCTACTTTGCAACAACAAGCCAATGTTGAATATGGATATACTTTGAAACAAACATTAAATATTTTAGAAA
GTTTGTACTTAAAAGGGATAACATCATATCCGAGAAGTAATATGGAATATTTACCGGCAGACTTAGAAGAATCGTTCTCT
GAAACACTAAAGAATTTATACTTAGCGTGGTTTATAGAAAAAGATGATATCGAAGGGGCTAAAAAGTGTATTAATGATGA
GAAAGCACAAAAAGATGCACACTATGCAATTATAATTGTAAATACTAAAACAAAAGAAATTGACAATTTGTCTAAAGATG
AAAGAAATATTTACGATTTAATTTCTAGAAGAATGATTGAAGCCACAATGAAAGATTTCGAATATATAGAACAAGATATT
CAGCTTAATGATGAGTTTTCTGGAAAAAAACGAAAATCTACACAAGAAGGTTTTAATAAAATTCAATACAATAAATTACA
ATACAATGATAATTTGGATTTAAACAAAGGTCAAAACTTCCAATGTGATCTTGAAATTAAGAAAACAAAAACTAAGCCTA
AACCAAGATTTACGGATGCAACAATTGTATCATCTATGGAAAATCTGAAAGACATTGACGAAAAATACAAAGGAGGAATT
GGAACTCCTGCAACTAGAGATTCAATAATAGAAAAACTTGTTGAATACAAGTATATAGAAAGAAGTAAAAAGACATTAAC
ATCAACTCCAATAGCAAGATTGTTGTACAAACTTTTATCAAAACAAATAAAAACTGTTGGTTTAACTGCTGCACTTGAAG
AAAAATTAGAAGAAGTAAGATTAGGTAATATAGAACAAGAAACCTTCTTAAATTTCATAGAAAAATATATATTAAATGAA
ATAGATAGGGTAAAAGATTTGGATGATTTAAGAATAGAAGTTTGCGATTGCCCTTTCTGTGGCAAAAAGGTCTTAAATCA
AGGGTTGAAATACGATTGCGAAAATGAAGATTGTAGTTTTGAGATATGGAAGAAAAATAAATATTTCGAAAGTTTCGGTA
TTAAAGAAAAATCAATAACAGATGATTTTGTAAAGCAATTATGTTCGTCTTCAGGGGTTATGGTAAAAGGATTAAAGAGT
AAAAAAGGCGAAAAATACAGTGCTATTTTTAGTGTAAATGAAGAAGATGGCAAAACAAATATATCTGTAGATTTCCCTAA
AAGAATGACTAAGAAATAA

Upstream 100 bases:

>100_bases
TGAATCGAATAAAAAGACAGTAGAACAAAAGACTGATGGCGAAGAACAAATTGAAGATGATTCGTTTAATGTAGAAACGC
CGCAATAATAGGAGAACAAA

Downstream 100 bases:

>100_bases
GGGGGTATTAAATGCAAGATAATAAAAAAACAGGATTTTTTGAAAAGATTTACAAAGCAACCTACGAAAATATGTCTGAT
GTTAAATTTGTGGGTTATGG

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MKLIIAEKKDMAQKIADSIGKFTPEDGYYDVLDCHVTWTWGHLFGLKEPNEYENQGWDKKWADSSIDDLPMIPNKYQYKL
TGNKDQFKKIEDLIKSNKYDEIINSCDAEREGELIFRLLYNHLDIDENKTKVTRMWCNTTNKDGLLKALEERKDIKEYDG
YYLEADSRSKADWLIGMNLSRLYMFKFFRKFVIGRVMTPTLCLIIERDLEIENFEESDIYNLVGEYNGFQFKRKFDTPEE
CDRFKDLKDFTVVDIVEEKKKKKAPKLFKLSTLQQQANVEYGYTLKQTLNILESLYLKGITSYPRSNMEYLPADLEESFS
ETLKNLYLAWFIEKDDIEGAKKCINDEKAQKDAHYAIIIVNTKTKEIDNLSKDERNIYDLISRRMIEATMKDFEYIEQDI
QLNDEFSGKKRKSTQEGFNKIQYNKLQYNDNLDLNKGQNFQCDLEIKKTKTKPKPRFTDATIVSSMENLKDIDEKYKGGI
GTPATRDSIIEKLVEYKYIERSKKTLTSTPIARLLYKLLSKQIKTVGLTAALEEKLEEVRLGNIEQETFLNFIEKYILNE
IDRVKDLDDLRIEVCDCPFCGKKVLNQGLKYDCENEDCSFEIWKKNKYFESFGIKEKSITDDFVKQLCSSSGVMVKGLKS
KKGEKYSAIFSVNEEDGKTNISVDFPKRMTKK

Sequences:

>Translated_672_residues
MKLIIAEKKDMAQKIADSIGKFTPEDGYYDVLDCHVTWTWGHLFGLKEPNEYENQGWDKKWADSSIDDLPMIPNKYQYKL
TGNKDQFKKIEDLIKSNKYDEIINSCDAEREGELIFRLLYNHLDIDENKTKVTRMWCNTTNKDGLLKALEERKDIKEYDG
YYLEADSRSKADWLIGMNLSRLYMFKFFRKFVIGRVMTPTLCLIIERDLEIENFEESDIYNLVGEYNGFQFKRKFDTPEE
CDRFKDLKDFTVVDIVEEKKKKKAPKLFKLSTLQQQANVEYGYTLKQTLNILESLYLKGITSYPRSNMEYLPADLEESFS
ETLKNLYLAWFIEKDDIEGAKKCINDEKAQKDAHYAIIIVNTKTKEIDNLSKDERNIYDLISRRMIEATMKDFEYIEQDI
QLNDEFSGKKRKSTQEGFNKIQYNKLQYNDNLDLNKGQNFQCDLEIKKTKTKPKPRFTDATIVSSMENLKDIDEKYKGGI
GTPATRDSIIEKLVEYKYIERSKKTLTSTPIARLLYKLLSKQIKTVGLTAALEEKLEEVRLGNIEQETFLNFIEKYILNE
IDRVKDLDDLRIEVCDCPFCGKKVLNQGLKYDCENEDCSFEIWKKNKYFESFGIKEKSITDDFVKQLCSSSGVMVKGLKS
KKGEKYSAIFSVNEEDGKTNISVDFPKRMTKK
>Mature_672_residues
MKLIIAEKKDMAQKIADSIGKFTPEDGYYDVLDCHVTWTWGHLFGLKEPNEYENQGWDKKWADSSIDDLPMIPNKYQYKL
TGNKDQFKKIEDLIKSNKYDEIINSCDAEREGELIFRLLYNHLDIDENKTKVTRMWCNTTNKDGLLKALEERKDIKEYDG
YYLEADSRSKADWLIGMNLSRLYMFKFFRKFVIGRVMTPTLCLIIERDLEIENFEESDIYNLVGEYNGFQFKRKFDTPEE
CDRFKDLKDFTVVDIVEEKKKKKAPKLFKLSTLQQQANVEYGYTLKQTLNILESLYLKGITSYPRSNMEYLPADLEESFS
ETLKNLYLAWFIEKDDIEGAKKCINDEKAQKDAHYAIIIVNTKTKEIDNLSKDERNIYDLISRRMIEATMKDFEYIEQDI
QLNDEFSGKKRKSTQEGFNKIQYNKLQYNDNLDLNKGQNFQCDLEIKKTKTKPKPRFTDATIVSSMENLKDIDEKYKGGI
GTPATRDSIIEKLVEYKYIERSKKTLTSTPIARLLYKLLSKQIKTVGLTAALEEKLEEVRLGNIEQETFLNFIEKYILNE
IDRVKDLDDLRIEVCDCPFCGKKVLNQGLKYDCENEDCSFEIWKKNKYFESFGIKEKSITDDFVKQLCSSSGVMVKGLKS
KKGEKYSAIFSVNEEDGKTNISVDFPKRMTKK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=628, Percent_Identity=21.656050955414, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI10835218, Length=730, Percent_Identity=22.0547945205479, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788061, Length=625, Percent_Identity=27.68, Blast_Score=198, Evalue=8e-52,
Organism=Escherichia coli, GI1787529, Length=529, Percent_Identity=23.062381852552, Blast_Score=98, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17555378, Length=548, Percent_Identity=22.8102189781022, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 78733; Mature: 78733

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIIAEKKDMAQKIADSIGKFTPEDGYYDVLDCHVTWTWGHLFGLKEPNEYENQGWDKK
CEEEEECHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEHHEECCCCCCHHHCCCCCCH
WADSSIDDLPMIPNKYQYKLTGNKDQFKKIEDLIKSNKYDEIINSCDAEREGELIFRLLY
HCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHH
NHLDIDENKTKVTRMWCNTTNKDGLLKALEERKDIKEYDGYYLEADSRSKADWLIGMNLS
HHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEECCCHH
RLYMFKFFRKFVIGRVMTPTLCLIIERDLEIENFEESDIYNLVGEYNGFQFKRKFDTPEE
HHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCHHH
CDRFKDLKDFTVVDIVEEKKKKKAPKLFKLSTLQQQANVEYGYTLKQTLNILESLYLKGI
HHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
TSYPRSNMEYLPADLEESFSETLKNLYLAWFIEKDDIEGAKKCINDEKAQKDAHYAIIIV
HCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHCHHHHCCCCCEEEEEE
NTKTKEIDNLSKDERNIYDLISRRMIEATMKDFEYIEQDIQLNDEFSGKKRKSTQEGFNK
ECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHH
IQYNKLQYNDNLDLNKGQNFQCDLEIKKTKTKPKPRFTDATIVSSMENLKDIDEKYKGGI
HCCCEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GTPATRDSIIEKLVEYKYIERSKKTLTSTPIARLLYKLLSKQIKTVGLTAALEEKLEEVR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGNIEQETFLNFIEKYILNEIDRVKDLDDLRIEVCDCPFCGKKVLNQGLKYDCENEDCSF
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCCE
EIWKKNKYFESFGIKEKSITDDFVKQLCSSSGVMVKGLKSKKGEKYSAIFSVNEEDGKTN
EEECCCCHHHHHCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEECCCCCCEE
ISVDFPKRMTKK
EEEECCHHHCCC
>Mature Secondary Structure
MKLIIAEKKDMAQKIADSIGKFTPEDGYYDVLDCHVTWTWGHLFGLKEPNEYENQGWDKK
CEEEEECHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEHHEECCCCCCHHHCCCCCCH
WADSSIDDLPMIPNKYQYKLTGNKDQFKKIEDLIKSNKYDEIINSCDAEREGELIFRLLY
HCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHH
NHLDIDENKTKVTRMWCNTTNKDGLLKALEERKDIKEYDGYYLEADSRSKADWLIGMNLS
HHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEECCCHH
RLYMFKFFRKFVIGRVMTPTLCLIIERDLEIENFEESDIYNLVGEYNGFQFKRKFDTPEE
HHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCHHH
CDRFKDLKDFTVVDIVEEKKKKKAPKLFKLSTLQQQANVEYGYTLKQTLNILESLYLKGI
HHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
TSYPRSNMEYLPADLEESFSETLKNLYLAWFIEKDDIEGAKKCINDEKAQKDAHYAIIIV
HCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHCHHHHCCCCCEEEEEE
NTKTKEIDNLSKDERNIYDLISRRMIEATMKDFEYIEQDIQLNDEFSGKKRKSTQEGFNK
ECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHH
IQYNKLQYNDNLDLNKGQNFQCDLEIKKTKTKPKPRFTDATIVSSMENLKDIDEKYKGGI
HCCCEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GTPATRDSIIEKLVEYKYIERSKKTLTSTPIARLLYKLLSKQIKTVGLTAALEEKLEEVR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGNIEQETFLNFIEKYILNEIDRVKDLDDLRIEVCDCPFCGKKVLNQGLKYDCENEDCSF
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCCE
EIWKKNKYFESFGIKEKSITDDFVKQLCSSSGVMVKGLKSKKGEKYSAIFSVNEEDGKTN
EEECCCCHHHHHCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEECCCCCCEE
ISVDFPKRMTKK
EEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA