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The map label for this gene is 169629631

Identifier: 169629631

GI number: 169629631

Start: 2592303

End: 2593286

Strand: Reverse

Name: 169629631

Synonym: MAB_2545c

Alternate gene names: NA

Gene position: 2593286-2592303 (Counterclockwise)

Preceding gene: 169629633

Following gene: 169629630

Centisome position: 51.18

GC content: 63.52

Gene sequence:

>984_bases
GTGCCCACATCGGGATTTGTCGTCGCAACCGCCTTCACACCCATGAGCTCGCCGGCCCGCGTGAGCGCCCCCCTTCGCAC
CCCGGTACGAGTAGGTCTGGTGCAGCACCGCTGGCTTGCCGATCCGGACCAGTTGCGCGACCAACTCTTGGAGGGCGTGC
GGCTTGCCGTCGCCCAGGGCGCACGCGCGGTGTTCCTGCCGGAGCTGACACTGTCGCGTTACCCCGCAGATGTACGCGCG
GGCACCAATCCGGGTGATCGAGCTGAGGATCTTTTAACCGGGCCGACGTTCTGCTTTGCCGCACACGCCGCCACGGAGAA
CGGCGTGCTGGTCCACGCGTCGCTGTATGAGCGTGCGGATGCTGCTGACGGTCTCGGCTACAACACCGCGATCCTGGTAT
CGCCCAGCGGAGAGCTTGTCGGTCGCACGCGCAAGCTACACATTCCCGTCACCGCGGGCTACTACGAGGACACCTACTTT
CGACCCGGACCCGCGGAGAACGCGTATCCGGTATATCGCCCCGCCGAACTCGACGGTGCGGCGGTCGGGATGCCCACCTG
TTGGGACGAATGGTTTCCCGAGGTCTCGCGCATGTACTCGCTGGCCGGTGCGGAAATCCTGGTTTACCCGACAGCGATCG
GTTCCGAACCGGCCTTCCCCTCATTTGATACGCAGCCGCTCTGGCAGCATGTGATCGTTGGCAACGGGATCACCGCAGGG
ACGTTCATGGTTGTTCCCAACCGTTTTGGTGATGAGGGAACCGTGAACTTCTATGGCTCATCGTTCATTTCCGACCCGTA
CGGCCGGATTCTCGCGCAGGCACCGCGCGACGCACCTGCGGTGTTGGTAGCGGATCTGGATCTTGAACAGCGCAAGGACT
GGCTAGATCTTTTCCCGTTCAACACGACCCGCCGACCCGATACTTACGCTCGGCTGGCAGCGCCCGTCGACACGTCACAC
CCCCATGGTTCGGAGGCGTCATGA

Upstream 100 bases:

>100_bases
TTTTCAGTTTGTTCTACCGTCACCGTAATGGCGATCATGCGGAGGCGGCACACCTACCCCACCGCCTACCAAGTCGCCTT
CCGCCCCAGGAGGTTAAGCA

Downstream 100 bases:

>100_bases
ACTGGCTCATGCCGCCTGAAACAGCTGCGCAGGAACGTATCTGGATGGCGTTTCCGCCGCAAGGGGCGAGCGTCTTCGAG
AACGCCGAGTCCGCACACGA

Product: nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MPTSGFVVATAFTPMSSPARVSAPLRTPVRVGLVQHRWLADPDQLRDQLLEGVRLAVAQGARAVFLPELTLSRYPADVRA
GTNPGDRAEDLLTGPTFCFAAHAATENGVLVHASLYERADAADGLGYNTAILVSPSGELVGRTRKLHIPVTAGYYEDTYF
RPGPAENAYPVYRPAELDGAAVGMPTCWDEWFPEVSRMYSLAGAEILVYPTAIGSEPAFPSFDTQPLWQHVIVGNGITAG
TFMVVPNRFGDEGTVNFYGSSFISDPYGRILAQAPRDAPAVLVADLDLEQRKDWLDLFPFNTTRRPDTYARLAAPVDTSH
PHGSEAS

Sequences:

>Translated_327_residues
MPTSGFVVATAFTPMSSPARVSAPLRTPVRVGLVQHRWLADPDQLRDQLLEGVRLAVAQGARAVFLPELTLSRYPADVRA
GTNPGDRAEDLLTGPTFCFAAHAATENGVLVHASLYERADAADGLGYNTAILVSPSGELVGRTRKLHIPVTAGYYEDTYF
RPGPAENAYPVYRPAELDGAAVGMPTCWDEWFPEVSRMYSLAGAEILVYPTAIGSEPAFPSFDTQPLWQHVIVGNGITAG
TFMVVPNRFGDEGTVNFYGSSFISDPYGRILAQAPRDAPAVLVADLDLEQRKDWLDLFPFNTTRRPDTYARLAAPVDTSH
PHGSEAS
>Mature_326_residues
PTSGFVVATAFTPMSSPARVSAPLRTPVRVGLVQHRWLADPDQLRDQLLEGVRLAVAQGARAVFLPELTLSRYPADVRAG
TNPGDRAEDLLTGPTFCFAAHAATENGVLVHASLYERADAADGLGYNTAILVSPSGELVGRTRKLHIPVTAGYYEDTYFR
PGPAENAYPVYRPAELDGAAVGMPTCWDEWFPEVSRMYSLAGAEILVYPTAIGSEPAFPSFDTQPLWQHVIVGNGITAGT
FMVVPNRFGDEGTVNFYGSSFISDPYGRILAQAPRDAPAVLVADLDLEQRKDWLDLFPFNTTRRPDTYARLAAPVDTSHP
HGSEAS

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI7706509, Length=324, Percent_Identity=27.7777777777778, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI297632348, Length=290, Percent_Identity=29.3103448275862, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI5031947, Length=290, Percent_Identity=29.3103448275862, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI297632350, Length=290, Percent_Identity=29.3103448275862, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI9910460, Length=296, Percent_Identity=26.3513513513513, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17533173, Length=335, Percent_Identity=27.1641791044776, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6323383, Length=216, Percent_Identity=25.9259259259259, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21358471, Length=311, Percent_Identity=27.9742765273312, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355835, Length=189, Percent_Identity=26.984126984127, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 35437; Mature: 35306

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTSGFVVATAFTPMSSPARVSAPLRTPVRVGLVQHRWLADPDQLRDQLLEGVRLAVAQG
CCCCCEEEEEEECCCCCCCEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
ARAVFLPELTLSRYPADVRAGTNPGDRAEDLLTGPTFCFAAHAATENGVLVHASLYERAD
CCEEEECCHHHHHCCCHHCCCCCCCCHHHHHHCCCCCEEEEECCCCCCEEEEEHHHHHCC
AADGLGYNTAILVSPSGELVGRTRKLHIPVTAGYYEDTYFRPGPAENAYPVYRPAELDGA
CCCCCCCCEEEEECCCCHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
AVGMPTCWDEWFPEVSRMYSLAGAEILVYPTAIGSEPAFPSFDTQPLWQHVIVGNGITAG
EECCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHEEECCCCCCC
TFMVVPNRFGDEGTVNFYGSSFISDPYGRILAQAPRDAPAVLVADLDLEQRKDWLDLFPF
EEEEECCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCEEEEECCCHHHCCCHHEECCC
NTTRRPDTYARLAAPVDTSHPHGSEAS
CCCCCCCHHHHEECCCCCCCCCCCCCC
>Mature Secondary Structure 
PTSGFVVATAFTPMSSPARVSAPLRTPVRVGLVQHRWLADPDQLRDQLLEGVRLAVAQG
CCCCEEEEEEECCCCCCCEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
ARAVFLPELTLSRYPADVRAGTNPGDRAEDLLTGPTFCFAAHAATENGVLVHASLYERAD
CCEEEECCHHHHHCCCHHCCCCCCCCHHHHHHCCCCCEEEEECCCCCCEEEEEHHHHHCC
AADGLGYNTAILVSPSGELVGRTRKLHIPVTAGYYEDTYFRPGPAENAYPVYRPAELDGA
CCCCCCCCEEEEECCCCHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
AVGMPTCWDEWFPEVSRMYSLAGAEILVYPTAIGSEPAFPSFDTQPLWQHVIVGNGITAG
EECCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHEEECCCCCCC
TFMVVPNRFGDEGTVNFYGSSFISDPYGRILAQAPRDAPAVLVADLDLEQRKDWLDLFPF
EEEEECCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCEEEEECCCHHHCCCHHEECCC
NTTRRPDTYARLAAPVDTSHPHGSEAS
CCCCCCCHHHHEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]