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The map label for this gene is ogt [H]

Identifier: 169629418

GI number: 169629418

Start: 2384936

End: 2385439

Strand: Reverse

Name: ogt [H]

Synonym: MAB_2332c

Alternate gene names: 169629418

Gene position: 2385439-2384936 (Counterclockwise)

Preceding gene: 169629419

Following gene: 169629415

Centisome position: 47.08

GC content: 64.68

Gene sequence:

>504_bases
GTGACCGATCATGCGACTCTCACCACCCCGGTGGGCCCATTTACCGCCATCGTGGATGATACGGGCGCCGTCTTGGCCTC
GGGGTGGACCGACTCACCTGAACTGCTCCGCGCACTGATTCACCCATCCCTGCGCCCCAAGACCCTGACGCCCAAACGCG
ATCTGGGAAAGGTCACCGAGGCGATCACCGACTATCACGCCGGCGACCTGACTTGTATCGACTCGATCCCTGTAGTTCAG
CGTTCCGGGGCGTTCCTCATGCATGCGTGGGATGTGCTGCGAACTGTAGATCCACAGGCACCCATCACCTACAGCGAGTT
CGCCGACCGCGCCGGGCGGCCCGCCGCCATCCGCGCCGCCGCAAGTGCCTGCGCACGGAACGCGGCGGCGTTGTTTGTGC
CATGCCACCGTGTTTTTCGGATCGGTGGCGCGCTAGGGGGCTTCCGCTATGGGCTCCCGATCAAGCGATGGCTACTTGAC
CATGAGGCCGCCGCCGTTCGCTGA

Upstream 100 bases:

>100_bases
GTGCTAGTTCGCCATGGCGCTCTTATGTCTCCATGCACCTGTGGCGCAAGGGAATCGCCGCGCTACCGCGGCAACTCACT
CGCAAACCGAAGGGAAAATC

Downstream 100 bases:

>100_bases
CCCAATGCGTGATCAGCACCGCAGCGAGCCCAGCCGGCAGGGCCACAGCCAGCGTTTGACCGTCCTCAGAGAACAGCATC
AACGCGAAAAGACAACCACA

Product: putative methylated-DNA:protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MTDHATLTTPVGPFTAIVDDTGAVLASGWTDSPELLRALIHPSLRPKTLTPKRDLGKVTEAITDYHAGDLTCIDSIPVVQ
RSGAFLMHAWDVLRTVDPQAPITYSEFADRAGRPAAIRAAASACARNAAALFVPCHRVFRIGGALGGFRYGLPIKRWLLD
HEAAAVR

Sequences:

>Translated_167_residues
MTDHATLTTPVGPFTAIVDDTGAVLASGWTDSPELLRALIHPSLRPKTLTPKRDLGKVTEAITDYHAGDLTCIDSIPVVQ
RSGAFLMHAWDVLRTVDPQAPITYSEFADRAGRPAAIRAAASACARNAAALFVPCHRVFRIGGALGGFRYGLPIKRWLLD
HEAAAVR
>Mature_166_residues
TDHATLTTPVGPFTAIVDDTGAVLASGWTDSPELLRALIHPSLRPKTLTPKRDLGKVTEAITDYHAGDLTCIDSIPVVQR
SGAFLMHAWDVLRTVDPQAPITYSEFADRAGRPAAIRAAASACARNAAALFVPCHRVFRIGGALGGFRYGLPIKRWLLDH
EAAAVR

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=87, Percent_Identity=37.9310344827586, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1787596, Length=159, Percent_Identity=31.4465408805031, Blast_Score=93, Evalue=7e-21,
Organism=Escherichia coli, GI1788542, Length=89, Percent_Identity=42.6966292134831, Blast_Score=81, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI115533068, Length=84, Percent_Identity=44.047619047619, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI115533070, Length=84, Percent_Identity=44.047619047619, Blast_Score=76, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17954; Mature: 17823

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDHATLTTPVGPFTAIVDDTGAVLASGWTDSPELLRALIHPSLRPKTLTPKRDLGKVTE
CCCCCEEECCCCCEEEEECCCCCEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AITDYHAGDLTCIDSIPVVQRSGAFLMHAWDVLRTVDPQAPITYSEFADRAGRPAAIRAA
HHHHCCCCCEEEECCCCCHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHH
ASACARNAAALFVPCHRVFRIGGALGGFRYGLPIKRWLLDHEAAAVR
HHHHHHCCCEEHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDHATLTTPVGPFTAIVDDTGAVLASGWTDSPELLRALIHPSLRPKTLTPKRDLGKVTE
CCCCEEECCCCCEEEEECCCCCEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AITDYHAGDLTCIDSIPVVQRSGAFLMHAWDVLRTVDPQAPITYSEFADRAGRPAAIRAA
HHHHCCCCCEEEECCCCCHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHH
ASACARNAAALFVPCHRVFRIGGALGGFRYGLPIKRWLLDHEAAAVR
HHHHHHCCCEEHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2825131; 9097039; 9278503; 1520330 [H]