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The map label for this gene is puuB [H]

Identifier: 169629369

GI number: 169629369

Start: 2330567

End: 2331985

Strand: Direct

Name: puuB [H]

Synonym: MAB_2283

Alternate gene names: 169629369

Gene position: 2330567-2331985 (Clockwise)

Preceding gene: 169629368

Following gene: 169629370

Centisome position: 45.99

GC content: 64.06

Gene sequence:

>1419_bases
ATGAACGGAACAGAAATCGGGCATCTTGCCGAAACGGCTCCCATTTGGTGGCCAGGTAGTCCGGAAAGCGCTGCCCCGAT
CACCGACGGCCGCCACGACGTCGACGTGCTCGTGATCGGGGCCGGGCTGGCCGGGCTCGCGGCCGCCTACTTCGTCACTG
AATTGCGTCCGGAACTGTCGGTGGCGGTGCTGGAGGCCAAACATGTCGGTGCCGGCGCCACCGGCCGCAGCACAGGGATC
GTTTCACCCGGCTTGAGCATGCCGATGGCCGCGTTCCGGCGGAAGTACGGCGATGACGCCGCCATCGCCTCGTTCGACGC
CACCTTTCACGGAGTGGGACTGCTGCGGGATCTGGTGGCCAAGGAGAACATCGACTGTGACGCCCGCGATGAGCCGCACA
CCCTTGTCGCCCTGACCGACCGCTCGCTGGCCCGCATGGTCACTCATTTGGAAAATCTCGCCGAACTGGGCCGCGAGGTG
CCCTGGCTGAGCTCCCAAGAAGTCGTCCAATGGGCCGGCGCCGGCTACAAGGCGGGATTTGCGTACCGCGATGCCATGGT
CGTGGACCCGTACCGGTTGGTCACCGGCATGGCCGACATCCTGCGCCGTCGCGGTGCCCCGGTGTACGAACACAGCCCCG
TGATCAGTATCGAGGCCGACGCCACCGGGGTGTGTGTATTCACCGCTCGCGCTCAGGTGCGGGCCCGGCACGCCTTCTTG
GCCACCGACGGCTATTCAGGCACTCTGAACCCACTGAAATCATCGGTTATCCCACTGCGCACCCATCTCTTGGCCACGGC
GCCGTTGACCGAGGGGCAACTCGCCGAACTGGGCTGGTCGGGTCGCGGCGCGATCATCGACCAACGGACCTTCTTCAACT
ATTATCGGATGTCCGCGGACAAGCGGGTGGTGTTCGGCGGCGGACCGGCGTTTGTGCCCTCGCGAATTCCTTCCCGAGAC
ATCAAGCGATCCAGGGTAATCCAGCAGCGTGTCCGTCGGGAACTGACCGAGCGATTCCCGGTTCTGGCCGATGTCGAGAT
TGACGCCGCCTGGTCAGGCATCACCGCCAGTTCTTTTGATCGCAACCCCGTGGTCGGTCCAGTACCTGGAATGGATGGCC
TCTGGTTCGCCGGAAGTTGGTGTGGGCATGGATTTTCCACCTCTGTAGATACGGGCTGGCGTTTCGCTAAGACCTTGGTG
GGGGAGCCGTTGCCCGCCAATATCCCTTGGTTCCGCTCCGGCGCTCAACGTGTGCCGACCACACTCCTACGCAACGTGGC
CACCGGTGCCTATTTGAAGGCCCTTGATGCCGCCGATGCGCTGGACGCCAAAACCAGCAACCCGCCGCAGGTCGCACGAC
CAACCGCGGGGCGACAGACATCAAGGGGCACGACAACATTGGCGGAGGCGGACAAATGA

Upstream 100 bases:

>100_bases
TTGGGCACGTGATCCATACCCACGAGGGCACCAAGGGCGCACCGCTGGTCTGGCATGACAGCAGCTTCCTGCCTGTCACC
GAACACCAAGAGAGGGACTA

Downstream 100 bases:

>100_bases
CGACGCAAGAACTGGCCCGTAATCATGACGTGATCATCGTGGGAGGCGGGATCGGCGGCTCGACCCTGGCAGCCATTTTG
GCGCGCCACGGGGTGCGGGT

Product: putative oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MNGTEIGHLAETAPIWWPGSPESAAPITDGRHDVDVLVIGAGLAGLAAAYFVTELRPELSVAVLEAKHVGAGATGRSTGI
VSPGLSMPMAAFRRKYGDDAAIASFDATFHGVGLLRDLVAKENIDCDARDEPHTLVALTDRSLARMVTHLENLAELGREV
PWLSSQEVVQWAGAGYKAGFAYRDAMVVDPYRLVTGMADILRRRGAPVYEHSPVISIEADATGVCVFTARAQVRARHAFL
ATDGYSGTLNPLKSSVIPLRTHLLATAPLTEGQLAELGWSGRGAIIDQRTFFNYYRMSADKRVVFGGGPAFVPSRIPSRD
IKRSRVIQQRVRRELTERFPVLADVEIDAAWSGITASSFDRNPVVGPVPGMDGLWFAGSWCGHGFSTSVDTGWRFAKTLV
GEPLPANIPWFRSGAQRVPTTLLRNVATGAYLKALDAADALDAKTSNPPQVARPTAGRQTSRGTTTLAEADK

Sequences:

>Translated_472_residues
MNGTEIGHLAETAPIWWPGSPESAAPITDGRHDVDVLVIGAGLAGLAAAYFVTELRPELSVAVLEAKHVGAGATGRSTGI
VSPGLSMPMAAFRRKYGDDAAIASFDATFHGVGLLRDLVAKENIDCDARDEPHTLVALTDRSLARMVTHLENLAELGREV
PWLSSQEVVQWAGAGYKAGFAYRDAMVVDPYRLVTGMADILRRRGAPVYEHSPVISIEADATGVCVFTARAQVRARHAFL
ATDGYSGTLNPLKSSVIPLRTHLLATAPLTEGQLAELGWSGRGAIIDQRTFFNYYRMSADKRVVFGGGPAFVPSRIPSRD
IKRSRVIQQRVRRELTERFPVLADVEIDAAWSGITASSFDRNPVVGPVPGMDGLWFAGSWCGHGFSTSVDTGWRFAKTLV
GEPLPANIPWFRSGAQRVPTTLLRNVATGAYLKALDAADALDAKTSNPPQVARPTAGRQTSRGTTTLAEADK
>Mature_472_residues
MNGTEIGHLAETAPIWWPGSPESAAPITDGRHDVDVLVIGAGLAGLAAAYFVTELRPELSVAVLEAKHVGAGATGRSTGI
VSPGLSMPMAAFRRKYGDDAAIASFDATFHGVGLLRDLVAKENIDCDARDEPHTLVALTDRSLARMVTHLENLAELGREV
PWLSSQEVVQWAGAGYKAGFAYRDAMVVDPYRLVTGMADILRRRGAPVYEHSPVISIEADATGVCVFTARAQVRARHAFL
ATDGYSGTLNPLKSSVIPLRTHLLATAPLTEGQLAELGWSGRGAIIDQRTFFNYYRMSADKRVVFGGGPAFVPSRIPSRD
IKRSRVIQQRVRRELTERFPVLADVEIDAAWSGITASSFDRNPVVGPVPGMDGLWFAGSWCGHGFSTSVDTGWRFAKTLV
GEPLPANIPWFRSGAQRVPTTLLRNVATGAYLKALDAADALDAKTSNPPQVARPTAGRQTSRGTTTLAEADK

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=414, Percent_Identity=28.743961352657, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 50697; Mature: 50697

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGTEIGHLAETAPIWWPGSPESAAPITDGRHDVDVLVIGAGLAGLAAAYFVTELRPELS
CCCCCCCCHHHCCCEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCE
VAVLEAKHVGAGATGRSTGIVSPGLSMPMAAFRRKYGDDAAIASFDATFHGVGLLRDLVA
EEEEEHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHEEECCCHHHHHHHHHHHHH
KENIDCDARDEPHTLVALTDRSLARMVTHLENLAELGREVPWLSSQEVVQWAGAGYKAGF
HCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCE
AYRDAMVVDPYRLVTGMADILRRRGAPVYEHSPVISIEADATGVCVFTARAQVRARHAFL
EECCEEEECHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEECCHHHHHHEEEE
ATDGYSGTLNPLKSSVIPLRTHLLATAPLTEGQLAELGWSGRGAIIDQRTFFNYYRMSAD
EECCCCCCCHHHHHHHHHHHHHHEEECCCCCCCHHHCCCCCCCCEEEHHHHHHHHHCCCC
KRVVFGGGPAFVPSRIPSRDIKRSRVIQQRVRRELTERFPVLADVEIDAAWSGITASSFD
CEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCC
RNPVVGPVPGMDGLWFAGSWCGHGFSTSVDTGWRFAKTLVGEPLPANIPWFRSGAQRVPT
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHCCHHHHHH
TLLRNVATGAYLKALDAADALDAKTSNPPQVARPTAGRQTSRGTTTLAEADK
HHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNGTEIGHLAETAPIWWPGSPESAAPITDGRHDVDVLVIGAGLAGLAAAYFVTELRPELS
CCCCCCCCHHHCCCEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCE
VAVLEAKHVGAGATGRSTGIVSPGLSMPMAAFRRKYGDDAAIASFDATFHGVGLLRDLVA
EEEEEHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHEEECCCHHHHHHHHHHHHH
KENIDCDARDEPHTLVALTDRSLARMVTHLENLAELGREVPWLSSQEVVQWAGAGYKAGF
HCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCE
AYRDAMVVDPYRLVTGMADILRRRGAPVYEHSPVISIEADATGVCVFTARAQVRARHAFL
EECCEEEECHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEECCHHHHHHEEEE
ATDGYSGTLNPLKSSVIPLRTHLLATAPLTEGQLAELGWSGRGAIIDQRTFFNYYRMSAD
EECCCCCCCHHHHHHHHHHHHHHEEECCCCCCCHHHCCCCCCCCEEEHHHHHHHHHCCCC
KRVVFGGGPAFVPSRIPSRDIKRSRVIQQRVRRELTERFPVLADVEIDAAWSGITASSFD
CEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCC
RNPVVGPVPGMDGLWFAGSWCGHGFSTSVDTGWRFAKTLVGEPLPANIPWFRSGAQRVPT
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHCCHHHHHH
TLLRNVATGAYLKALDAADALDAKTSNPPQVARPTAGRQTSRGTTTLAEADK
HHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]