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The map label for this gene is proP [H]

Identifier: 169629349

GI number: 169629349

Start: 2309890

End: 2311281

Strand: Reverse

Name: proP [H]

Synonym: MAB_2263c

Alternate gene names: 169629349

Gene position: 2311281-2309890 (Counterclockwise)

Preceding gene: 169629350

Following gene: 169629348

Centisome position: 45.61

GC content: 63.65

Gene sequence:

>1392_bases
ATGTCGTCCGCCGGCCCACGCGAGGGTGAAACGGAACCTCCCCCGGTCCCTCGACCGGATTTCCAATCACCCGAGGGCCG
GCGGCAACTTCACCGCGCGATCGCGGCATCGGCCATGGGTAATGCCACCGAGTGGTACGACTACGGCGTCTACGCTGCCA
CCACCACCTTTCTGACGCAGGCATTTTTTCCGACCCTGGGCACGGCGTTCACCATGCTCGGGTATGCGATCTCCTTCCTG
TTGCGGCCACTGGGCGGAATGGTTTGGGGGCCACTGGGCGACCGACTGGGACGCAAGAGCGTCATGGCCATCACCATCGT
GCTGATGGCCTTGTCCACCACCCTCATCGGCATCTTGCCCAGCTGGGCGACCATCGGGATGGCCGCGCCCGCGCTCTTGA
TCCTCCTGCGCGTCATACAGGGCTTCTCCACCGGTGGCGAATACGGCGGTGCGGCAACGTTCATGGCCGAGTTCGCGCCC
GACAAGAAGCGCGGCAAGTATGGCTCTTTCCTCGAATTCGGCACCCTGGGTGGGTTCGCCGTCGGGGCCGCGTTCGTCCT
CGTCCTGGACGCCACCCTCAGCGACGACGCCATGCACACCTGGGGCTGGCGGATACCCTTTCTCGTCGCGCTTCCGATGG
GACTGATCGGCTGGTACCTGCGCAGCCGCCTCGACGATCCGCCGTTGTTCAAGGAGATGCAGGAGCAGGAGCCGGAGGGG
CGCGAGACGGCCTTTGAGCGGCTCAGGGATCTGGTGCGCGACTACCGGCGGCCCATGGTCGTCATGATGCTCTTGGTCAT
CGCGCTCAACATCACCAACTACACACTGCTCAGCTATCAGCCCACATACCTCAAGACCAGGCTCGGAATGTCCGAGACTC
AAGGCCAGATCGTGATCCTCATCGGCGAAGTCGCCATGATGGCGTTCCTGCCCTTCTGCGGTGCGCTGTCGGACCGCGTA
GGGCGTAAACCGATGTGGGCATTCTCGCTGACCGGACTACTGGTGCTGTCACTACCCATGTTCTGGCTCATGGGGCAGGG
ATTCGGTTGGGCGATAGTTGGTTTCGCTGTCCTGGGATTGCTGTACATACCGCAGCTCTCGACCATCACCGCAACCTTCC
CTTGCATGTTCCCGACCCAGGTGCGCTATGCCGGTTTCGCAATCTCGTACAACCTGTCCACGGCGGCATTCGGCGGGACC
GCGCCGCTGGTCAATGACCTGGTGGTGGCTAGAACCGGCTGGGACTTGTTTCCCGCCGCCTACCTCATGGTGGCCTGTGT
CGTCGGGCTGTGCGCGCTGCCCTTCCTCATCGAAACGGCCGGGGCCTCGATCGCCGGAACCGAGATCCCCTGCAATGAGG
CCGCCGTGCCTACCGACACCGTTCGAGCCTGA

Upstream 100 bases:

>100_bases
ACACGCCACAGAAACCCTTCGCGGGCTACGAGCCGCGGCCGGAATACATCGCGGCGCGAGACTTCACCCCGCTGACTCCC
AAATCACCGAGCACGCCCTG

Downstream 100 bases:

>100_bases
CCTAGCCCCTACCTCTTAGGTTAGGATGGGCTAAGTACTGGCGGGCACGAACAAAGGTCACTTAAACATGGGTAGAGGGT
TCCAGGGCGCGATACTGCGC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MSSAGPREGETEPPPVPRPDFQSPEGRRQLHRAIAASAMGNATEWYDYGVYAATTTFLTQAFFPTLGTAFTMLGYAISFL
LRPLGGMVWGPLGDRLGRKSVMAITIVLMALSTTLIGILPSWATIGMAAPALLILLRVIQGFSTGGEYGGAATFMAEFAP
DKKRGKYGSFLEFGTLGGFAVGAAFVLVLDATLSDDAMHTWGWRIPFLVALPMGLIGWYLRSRLDDPPLFKEMQEQEPEG
RETAFERLRDLVRDYRRPMVVMMLLVIALNITNYTLLSYQPTYLKTRLGMSETQGQIVILIGEVAMMAFLPFCGALSDRV
GRKPMWAFSLTGLLVLSLPMFWLMGQGFGWAIVGFAVLGLLYIPQLSTITATFPCMFPTQVRYAGFAISYNLSTAAFGGT
APLVNDLVVARTGWDLFPAAYLMVACVVGLCALPFLIETAGASIAGTEIPCNEAAVPTDTVRA

Sequences:

>Translated_463_residues
MSSAGPREGETEPPPVPRPDFQSPEGRRQLHRAIAASAMGNATEWYDYGVYAATTTFLTQAFFPTLGTAFTMLGYAISFL
LRPLGGMVWGPLGDRLGRKSVMAITIVLMALSTTLIGILPSWATIGMAAPALLILLRVIQGFSTGGEYGGAATFMAEFAP
DKKRGKYGSFLEFGTLGGFAVGAAFVLVLDATLSDDAMHTWGWRIPFLVALPMGLIGWYLRSRLDDPPLFKEMQEQEPEG
RETAFERLRDLVRDYRRPMVVMMLLVIALNITNYTLLSYQPTYLKTRLGMSETQGQIVILIGEVAMMAFLPFCGALSDRV
GRKPMWAFSLTGLLVLSLPMFWLMGQGFGWAIVGFAVLGLLYIPQLSTITATFPCMFPTQVRYAGFAISYNLSTAAFGGT
APLVNDLVVARTGWDLFPAAYLMVACVVGLCALPFLIETAGASIAGTEIPCNEAAVPTDTVRA
>Mature_462_residues
SSAGPREGETEPPPVPRPDFQSPEGRRQLHRAIAASAMGNATEWYDYGVYAATTTFLTQAFFPTLGTAFTMLGYAISFLL
RPLGGMVWGPLGDRLGRKSVMAITIVLMALSTTLIGILPSWATIGMAAPALLILLRVIQGFSTGGEYGGAATFMAEFAPD
KKRGKYGSFLEFGTLGGFAVGAAFVLVLDATLSDDAMHTWGWRIPFLVALPMGLIGWYLRSRLDDPPLFKEMQEQEPEGR
ETAFERLRDLVRDYRRPMVVMMLLVIALNITNYTLLSYQPTYLKTRLGMSETQGQIVILIGEVAMMAFLPFCGALSDRVG
RKPMWAFSLTGLLVLSLPMFWLMGQGFGWAIVGFAVLGLLYIPQLSTITATFPCMFPTQVRYAGFAISYNLSTAAFGGTA
PLVNDLVVARTGWDLFPAAYLMVACVVGLCALPFLIETAGASIAGTEIPCNEAAVPTDTVRA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=428, Percent_Identity=39.018691588785, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1788942, Length=401, Percent_Identity=33.1670822942643, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1788292, Length=430, Percent_Identity=30.9302325581395, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789941, Length=385, Percent_Identity=31.6883116883117, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI87082231, Length=440, Percent_Identity=24.7727272727273, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082404, Length=341, Percent_Identity=24.3401759530792, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 50227; Mature: 50095

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCCC
>Mature Secondary Structure 
SSAGPREGETEPPPVPRPDFQSPEGRRQLHRAIAASAMGNATEWYDYGVYAATTTFLTQ
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHH
AFFPTLGTAFTMLGYAISFLLRPLGGMVWGPLGDRLGRKSVMAITIVLMALSTTLIGILP
HHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
SWATIGMAAPALLILLRVIQGFSTGGEYGGAATFMAEFAPDKKRGKYGSFLEFGTLGGFA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCHHH
VGAAFVLVLDATLSDDAMHTWGWRIPFLVALPMGLIGWYLRSRLDDPPLFKEMQEQEPEG
HHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCH
RETAFERLRDLVRDYRRPMVVMMLLVIALNITNYTLLSYQPTYLKTRLGMSETQGQIVIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHCCCCCCCCCEEEE
IGEVAMMAFLPFCGALSDRVGRKPMWAFSLTGLLVLSLPMFWLMGQGFGWAIVGFAVLGL
EHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LYIPQLSTITATFPCMFPTQVRYAGFAISYNLSTAAFGGTAPLVNDLVVARTGWDLFPAA
HHHCCHHHHHHHCCCCCCCHHHHEEEEEEECCCHHCCCCCHHHHHHHHHHCCCCHHHHHH
YLMVACVVGLCALPFLIETAGASIAGTEIPCNEAAVPTDTVRA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]