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The map label for this gene is mbtG [H]

Identifier: 169629335

GI number: 169629335

Start: 2280481

End: 2281770

Strand: Direct

Name: mbtG [H]

Synonym: MAB_2249

Alternate gene names: 169629335

Gene position: 2280481-2281770 (Clockwise)

Preceding gene: 169629334

Following gene: 169629336

Centisome position: 45.01

GC content: 63.88

Gene sequence:

>1290_bases
GTGACGTCGACATTGGCAGTGATAGGTGCGGGCGCCAAGGCGGTCGCGGTGGCCGCGAAGGCAGCCGTACTGCGCGAATG
CGGTGTCGACGTGCCCGACGTGGTGGCGATCGAACGCCAGCAGGTTGGCGCCAACTGGCAGGCGTGCGGCGGCTGGACCG
ATGGGCAGCACCGACTGGGCACCAGCCCCGAGAAGGATGTGGGCTTTCCGTATCGATCCGCCATTCTTCCGGGGCGTAAC
GCCGAGATCGACGCCCGGATGATGCGGCTGAGCTGGCAGTCGTACCTGGTCGCCACCGGTCAATTCGCCGAATGGGTCGA
CCGCGATAAGCCGGCGCCGACGCACAAGCGGTGGGCCGACTACCTGACATGGGTGGCCAACACGGTCGATATGAAGGTCG
TACACGGTGAAGTGACGGGATTGTCGATTGACGACGACCGATGGGTTGTCGGCACCAAGGACTGTGCCATCGCGGCCGAT
GCCGTGATGGTGACGGGGCCCGGTCAACCGGAACGTTCCGTGCTGCCCGAGCATCCAAATGTCTTGTCCATCGCTGAATT
CTGGCGTCGCTGTGCCGGCCCCATGCGGCTCACCGCTGAGCGCGTTGCCGTGATCGGTGGCGGAGAGACCGCCGCCTCGG
TACTCAACGAACTATTCGGACACCGGGTTTCCACCGTCACCGTCATCTCGCCAGGCGTCACGCTGTTTACCCGTGGTGAA
GGGTACTTCGAGAACGCGCTGTTCAGTGATCCGACCGGATGGCTGCACCACACGATGGAGGAGCGCCGCGACGCGCTGAA
CCGGACCGACCGTGGGGTTTTCTCAGCACGTGTGCAGGACTCGCTGCTGGCCGACGACCGCATCCGGCATCTGCGCGGCC
GGGTCGCTCATGCGGTCGCGCTGGATGACCGGATCAGGCTGACGCTGCGCAGCGACCGTGCTGGGGAGCCGTCCGAAACC
GTGCACGGCTTCGATCTGGTGATCGACGGGGCGGGCGCTGACGCACTCTGGTTCCTGCCGTTGTTCGACCAGAGTGCCCT
TGATCTCATGGAGCTCAAGCTCGGTGCTCCGGTGGACGGCAACGTGTTGCAGGAATCGCTCGGACACGATCTGTCCGTCA
ACGGCCTCACGCCCAAGCTCATTCTTCCGACGCTTTCGGGTCTGACTCAGGGGCCGGGGTTTCCCAATCTCAGCTGTCTC
GGGTTGTTGTCAGATCGAGTGCTCGGTGGGCACAGCGGCACCGATTCGGTTCGTCGTGAAAGGAAAATTGTTGAGTACCA
ACCCATTTGA

Upstream 100 bases:

>100_bases
CCTTGTGGCGCGCGATTCCGGAGGTACTCAGTCGCGCGGAGGTCACCCAACTGCAAGCGATATGGCACGAACAGCTGGGA
ATTCTGGCAAAGGAGCAATG

Downstream 100 bases:

>100_bases
TGACGAGAACGGCACCTTCTACGTTCTGGTCAACGACGAGGAGCAGTACAGTTTGTGGCCCAACGCCATCGAGATCCCGG
AGCGGTGGCGAGTGGTCTTC

Product: lysine-N-oxygenase MbtG

Products: NA

Alternate protein names: Lysine 6-N-hydroxylase; Lysine N(6)-hydroxylase; Lysine-N-oxygenase; Mycobactin synthase protein G [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTSTLAVIGAGAKAVAVAAKAAVLRECGVDVPDVVAIERQQVGANWQACGGWTDGQHRLGTSPEKDVGFPYRSAILPGRN
AEIDARMMRLSWQSYLVATGQFAEWVDRDKPAPTHKRWADYLTWVANTVDMKVVHGEVTGLSIDDDRWVVGTKDCAIAAD
AVMVTGPGQPERSVLPEHPNVLSIAEFWRRCAGPMRLTAERVAVIGGGETAASVLNELFGHRVSTVTVISPGVTLFTRGE
GYFENALFSDPTGWLHHTMEERRDALNRTDRGVFSARVQDSLLADDRIRHLRGRVAHAVALDDRIRLTLRSDRAGEPSET
VHGFDLVIDGAGADALWFLPLFDQSALDLMELKLGAPVDGNVLQESLGHDLSVNGLTPKLILPTLSGLTQGPGFPNLSCL
GLLSDRVLGGHSGTDSVRRERKIVEYQPI

Sequences:

>Translated_429_residues
MTSTLAVIGAGAKAVAVAAKAAVLRECGVDVPDVVAIERQQVGANWQACGGWTDGQHRLGTSPEKDVGFPYRSAILPGRN
AEIDARMMRLSWQSYLVATGQFAEWVDRDKPAPTHKRWADYLTWVANTVDMKVVHGEVTGLSIDDDRWVVGTKDCAIAAD
AVMVTGPGQPERSVLPEHPNVLSIAEFWRRCAGPMRLTAERVAVIGGGETAASVLNELFGHRVSTVTVISPGVTLFTRGE
GYFENALFSDPTGWLHHTMEERRDALNRTDRGVFSARVQDSLLADDRIRHLRGRVAHAVALDDRIRLTLRSDRAGEPSET
VHGFDLVIDGAGADALWFLPLFDQSALDLMELKLGAPVDGNVLQESLGHDLSVNGLTPKLILPTLSGLTQGPGFPNLSCL
GLLSDRVLGGHSGTDSVRRERKIVEYQPI
>Mature_428_residues
TSTLAVIGAGAKAVAVAAKAAVLRECGVDVPDVVAIERQQVGANWQACGGWTDGQHRLGTSPEKDVGFPYRSAILPGRNA
EIDARMMRLSWQSYLVATGQFAEWVDRDKPAPTHKRWADYLTWVANTVDMKVVHGEVTGLSIDDDRWVVGTKDCAIAADA
VMVTGPGQPERSVLPEHPNVLSIAEFWRRCAGPMRLTAERVAVIGGGETAASVLNELFGHRVSTVTVISPGVTLFTRGEG
YFENALFSDPTGWLHHTMEERRDALNRTDRGVFSARVQDSLLADDRIRHLRGRVAHAVALDDRIRLTLRSDRAGEPSETV
HGFDLVIDGAGADALWFLPLFDQSALDLMELKLGAPVDGNVLQESLGHDLSVNGLTPKLILPTLSGLTQGPGFPNLSCLG
LLSDRVLGGHSGTDSVRRERKIVEYQPI

Specific function: Flavoprotein monooxygenase required for N-hydroxylation of the two acylated lysine residues during mycobactin assembly, thus producing the hydroxamate groups necessary for iron sequestration. Is also able, but less efficiently, to hydroxylate L-lysine (no

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027 [H]

Pfam domain/function: PF07992 Pyr_redox_2 [H]

EC number: =1.14.13.59 [H]

Molecular weight: Translated: 46518; Mature: 46387

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTLAVIGAGAKAVAVAAKAAVLRECGVDVPDVVAIERQQVGANWQACGGWTDGQHRLG
CCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHEEHHHHHCCCCEECCCCCCCCCCCC
TSPEKDVGFPYRSAILPGRNAEIDARMMRLSWQSYLVATGQFAEWVDRDKPAPTHKRWAD
CCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHEEECCHHHHHHHCCCCCCCHHHHHH
YLTWVANTVDMKVVHGEVTGLSIDDDRWVVGTKDCAIAADAVMVTGPGQPERSVLPEHPN
HHHHHHCCEEEEEEEEEEEEEEECCCCEEEECCCCEEEECEEEEECCCCCCCCCCCCCCC
VLSIAEFWRRCAGPMRLTAERVAVIGGGETAASVLNELFGHRVSTVTVISPGVTLFTRGE
EEHHHHHHHHCCCCCEEEEEEEEEEECCHHHHHHHHHHHCCCEEEEEEEECCEEEEECCC
GYFENALFSDPTGWLHHTMEERRDALNRTDRGVFSARVQDSLLADDRIRHLRGRVAHAVA
CHHHCCCCCCCCHHHHHHHHHHHHHHCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LDDRIRLTLRSDRAGEPSETVHGFDLVIDGAGADALWFLPLFDQSALDLMELKLGAPVDG
ECCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCCC
NVLQESLGHDLSVNGLTPKLILPTLSGLTQGPGFPNLSCLGLLSDRVLGGHSGTDSVRRE
HHHHHHCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHH
RKIVEYQPI
HHCCCCCCC
>Mature Secondary Structure 
TSTLAVIGAGAKAVAVAAKAAVLRECGVDVPDVVAIERQQVGANWQACGGWTDGQHRLG
CCCEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHEEHHHHHCCCCEECCCCCCCCCCCC
TSPEKDVGFPYRSAILPGRNAEIDARMMRLSWQSYLVATGQFAEWVDRDKPAPTHKRWAD
CCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHEEECCHHHHHHHCCCCCCCHHHHHH
YLTWVANTVDMKVVHGEVTGLSIDDDRWVVGTKDCAIAADAVMVTGPGQPERSVLPEHPN
HHHHHHCCEEEEEEEEEEEEEEECCCCEEEECCCCEEEECEEEEECCCCCCCCCCCCCCC
VLSIAEFWRRCAGPMRLTAERVAVIGGGETAASVLNELFGHRVSTVTVISPGVTLFTRGE
EEHHHHHHHHCCCCCEEEEEEEEEEECCHHHHHHHHHHHCCCEEEEEEEECCEEEEECCC
GYFENALFSDPTGWLHHTMEERRDALNRTDRGVFSARVQDSLLADDRIRHLRGRVAHAVA
CHHHCCCCCCCCHHHHHHHHHHHHHHCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LDDRIRLTLRSDRAGEPSETVHGFDLVIDGAGADALWFLPLFDQSALDLMELKLGAPVDG
ECCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCCC
NVLQESLGHDLSVNGLTPKLILPTLSGLTQGPGFPNLSCLGLLSDRVLGGHSGTDSVRRE
HHHHHHCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHH
RKIVEYQPI
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA