Click here to switch to the map view.

The map label for this gene is cocE [H]

Identifier: 169629308

GI number: 169629308

Start: 2244103

End: 2245866

Strand: Reverse

Name: cocE [H]

Synonym: MAB_2222c

Alternate gene names: 169629308

Gene position: 2245866-2244103 (Counterclockwise)

Preceding gene: 169629310

Following gene: 169629306

Centisome position: 44.32

GC content: 65.19

Gene sequence:

>1764_bases
GTGCCCGCCACCATGCGCGACGGCACCATCCTGCGTGCCGATATCTACCGCCCGAAGCTCAAAGACCCGGTGCCGGTCAT
CTTGATGCGCACTCAGTACGGCAAGGACACCGCACAAATTCAGCCGTCGCGATTTCGCACCCCGGATTGGTTCGCCTCTC
ACTGCTATCTGGTGGTCGTACAGGACGTGCGAGGGCAGGGCACTTCGGACGGCATTTTCACCGAGTTCGGCAATGACGGC
GACGACGGCTATGACACCGTGGAGTGGGCGGCAGGGCTTCCCGGCGCCACCGGCAAGGTCGGCATGTACGGCTCGTCGTA
TGTGGGTGCGACCCAATGGCTCGCCGCCACCACGACTCCCCCACACCTGACGACCATCGTCCCCTCCAACACCGCATCCG
ACTACTACGACGGCTGGACCTACGAGGGCGGCGCGTTCCGGCTCGGGTTCGTGCAGCCGTGGGCCATGGGTACGATCGCC
ACCACCGCGGCGAAGAACCGGGCCGACCTCGCTGCCCTGGAGCAGCTACGGGCCGCCGCGGCCGAACGCGGCCGTTGGCT
CAATCACCTTCCCTACCAGGATCTTCCACCGATGCAACCCGGAGACCCCCGGGTGGCTCCGTGGTACTTCGACTGGATCC
GGCATTCGACCCGGGACGACTACTGGAAGCGTTGGAGCATCAGGGACCGGTATCAGAATGTCACGGTTCCGGTGCTGCAT
TTCGAAGGCTGGTACGACGCATTCCTACGCGGGGGTCTGGAGAACTTCGCAGGCATGACCGCCAGGGGCGGTTCGCCGGG
GGCACGGCGCTATCAGCGCATGGTCATCGGCCCGTGGGATCATCTGGCCTGGGGGCGCGAATCATCAAGCCCGTCACCGC
TGTTGAAGGGACTGGGGCCGGGCGCCAACAGCCCCGTCAACGAAATGCAGCTAGCGTGGTTCGATCATTTCCTCAAGGGC
TCGGACAATGGCGTAGCGACCGAGTCGCCGCGGCTCGACTATTACCTGATGGGAGCCAACCGCTGGAAGCACGCGTCCGC
TTGGCCCCTACCTCAGACGAATTGGACCGTATACCACCTTGGCGGGCGCGGCACGCAGCACAACGGGACACTGCGTGACT
CGGCTGCCGCGCACGACGCACCCGATGTCTATCTCTATGACCCGGCCAATCCAGTGCCGAGCGCCGGCGGACACTCCTGC
TGCGCCGCCGAGAGCGCACCGCAGGGCCGCTACGACCAGCGCGCCGTGGAGCAGCGGCCGGATGTGCTGACGTACACCAC
CGAGCCACTGAGCACCAATACCGAGATCACCGGCCCGATCAGTGTCGACCTATGGGCGTCGTCGTCGGCGCCAGACACCG
ACTTCACCGCGAAACTTGTTGCCGTGCAAGCCGATGGCACAGCGGCGAACCTCAACAACGGCATCGTGCGCACATCCTTG
GCCCAATCCCTGTCCGAGCGCCGTCTGATGCCGATAGGCCAGCCCCATAAGTACCACATCGACATCTGGCCGGTGAGTTA
CCAGTTCCGCACCGGCGAGCGCATTCGGCTGGAGATCTCCAGCAGCGACTTTCCGCAGTACGCCCCGAATCCGAACACCG
CCGCACCGTTCGGGCGGAATGCCGAGCTGCGCACCGCGACACAAATAATCCTGCACGATGCGGCGCACCCGTCAACGCTC
ACCCTGCCGGTGATACCGGCCGGTGACCCGGGAACCGAGACCTTCCCGATGCCCCGCCGACCGGATGACCCACCCGGAAA
CTAG

Upstream 100 bases:

>100_bases
GCTGAGCCTGGTCCTCACGGGGTGTGCTCAGGGCACCACGTTCAACTCATGGCCACCTTCGGATGACCGGGGCCCATGCG
CGGTCGCCAAGAAATCCGAT

Downstream 100 bases:

>100_bases
CCCCAGTTCTCCGGGCCGCCGAGGTCCGCCGAGAGCCAGTGCTCCGGCGAGACGTACACAGCGATCTGCTCACCGTAGCT
TTCGGCGTATTTGAGGTAGC

Product: peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MPATMRDGTILRADIYRPKLKDPVPVILMRTQYGKDTAQIQPSRFRTPDWFASHCYLVVVQDVRGQGTSDGIFTEFGNDG
DDGYDTVEWAAGLPGATGKVGMYGSSYVGATQWLAATTTPPHLTTIVPSNTASDYYDGWTYEGGAFRLGFVQPWAMGTIA
TTAAKNRADLAALEQLRAAAAERGRWLNHLPYQDLPPMQPGDPRVAPWYFDWIRHSTRDDYWKRWSIRDRYQNVTVPVLH
FEGWYDAFLRGGLENFAGMTARGGSPGARRYQRMVIGPWDHLAWGRESSSPSPLLKGLGPGANSPVNEMQLAWFDHFLKG
SDNGVATESPRLDYYLMGANRWKHASAWPLPQTNWTVYHLGGRGTQHNGTLRDSAAAHDAPDVYLYDPANPVPSAGGHSC
CAAESAPQGRYDQRAVEQRPDVLTYTTEPLSTNTEITGPISVDLWASSSAPDTDFTAKLVAVQADGTAANLNNGIVRTSL
AQSLSERRLMPIGQPHKYHIDIWPVSYQFRTGERIRLEISSSDFPQYAPNPNTAAPFGRNAELRTATQIILHDAAHPSTL
TLPVIPAGDPGTETFPMPRRPDDPPGN

Sequences:

>Translated_587_residues
MPATMRDGTILRADIYRPKLKDPVPVILMRTQYGKDTAQIQPSRFRTPDWFASHCYLVVVQDVRGQGTSDGIFTEFGNDG
DDGYDTVEWAAGLPGATGKVGMYGSSYVGATQWLAATTTPPHLTTIVPSNTASDYYDGWTYEGGAFRLGFVQPWAMGTIA
TTAAKNRADLAALEQLRAAAAERGRWLNHLPYQDLPPMQPGDPRVAPWYFDWIRHSTRDDYWKRWSIRDRYQNVTVPVLH
FEGWYDAFLRGGLENFAGMTARGGSPGARRYQRMVIGPWDHLAWGRESSSPSPLLKGLGPGANSPVNEMQLAWFDHFLKG
SDNGVATESPRLDYYLMGANRWKHASAWPLPQTNWTVYHLGGRGTQHNGTLRDSAAAHDAPDVYLYDPANPVPSAGGHSC
CAAESAPQGRYDQRAVEQRPDVLTYTTEPLSTNTEITGPISVDLWASSSAPDTDFTAKLVAVQADGTAANLNNGIVRTSL
AQSLSERRLMPIGQPHKYHIDIWPVSYQFRTGERIRLEISSSDFPQYAPNPNTAAPFGRNAELRTATQIILHDAAHPSTL
TLPVIPAGDPGTETFPMPRRPDDPPGN
>Mature_586_residues
PATMRDGTILRADIYRPKLKDPVPVILMRTQYGKDTAQIQPSRFRTPDWFASHCYLVVVQDVRGQGTSDGIFTEFGNDGD
DGYDTVEWAAGLPGATGKVGMYGSSYVGATQWLAATTTPPHLTTIVPSNTASDYYDGWTYEGGAFRLGFVQPWAMGTIAT
TAAKNRADLAALEQLRAAAAERGRWLNHLPYQDLPPMQPGDPRVAPWYFDWIRHSTRDDYWKRWSIRDRYQNVTVPVLHF
EGWYDAFLRGGLENFAGMTARGGSPGARRYQRMVIGPWDHLAWGRESSSPSPLLKGLGPGANSPVNEMQLAWFDHFLKGS
DNGVATESPRLDYYLMGANRWKHASAWPLPQTNWTVYHLGGRGTQHNGTLRDSAAAHDAPDVYLYDPANPVPSAGGHSCC
AAESAPQGRYDQRAVEQRPDVLTYTTEPLSTNTEITGPISVDLWASSSAPDTDFTAKLVAVQADGTAANLNNGIVRTSLA
QSLSERRLMPIGQPHKYHIDIWPVSYQFRTGERIRLEISSSDFPQYAPNPNTAAPFGRNAELRTATQIILHDAAHPSTLT
LPVIPAGDPGTETFPMPRRPDDPPGN

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 64728; Mature: 64596

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATMRDGTILRADIYRPKLKDPVPVILMRTQYGKDTAQIQPSRFRTPDWFASHCYLVVV
CCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCEECCCCCCCCCHHCCCEEEEEE
QDVRGQGTSDGIFTEFGNDGDDGYDTVEWAAGLPGATGKVGMYGSSYVGATQWLAATTTP
EECCCCCCCCCEEEECCCCCCCCCCHHHHHCCCCCCCCCEECCCCCCCCHHHEEEECCCC
PHLTTIVPSNTASDYYDGWTYEGGAFRLGFVQPWAMGTIATTAAKNRADLAALEQLRAAA
CCEEEEECCCCCCCCCCCEEECCCCEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHHH
AERGRWLNHLPYQDLPPMQPGDPRVAPWYFDWIRHSTRDDYWKRWSIRDRYQNVTVPVLH
HHHCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHCCCHHHHHCCCEEEEEE
FEGWYDAFLRGGLENFAGMTARGGSPGARRYQRMVIGPWDHLAWGRESSSPSPLLKGLGP
ECHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHEEEECCHHHHCCCCCCCCCCHHHHHCCC
GANSPVNEMQLAWFDHFLKGSDNGVATESPRLDYYLMGANRWKHASAWPLPQTNWTVYHL
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEC
GGRGTQHNGTLRDSAAAHDAPDVYLYDPANPVPSAGGHSCCAAESAPQGRYDQRAVEQRP
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHCC
DVLTYTTEPLSTNTEITGPISVDLWASSSAPDTDFTAKLVAVQADGTAANLNNGIVRTSL
CEEEEECCCCCCCCEECCCEEEEEEECCCCCCCCCEEEEEEEECCCCEECCCCCCHHHHH
AQSLSERRLMPIGQPHKYHIDIWPVSYQFRTGERIRLEISSSDFPQYAPNPNTAAPFGRN
HHHHHHCCCCCCCCCCEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
AELRTATQIILHDAAHPSTLTLPVIPAGDPGTETFPMPRRPDDPPGN
CCHHHHEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PATMRDGTILRADIYRPKLKDPVPVILMRTQYGKDTAQIQPSRFRTPDWFASHCYLVVV
CCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCEECCCCCCCCCHHCCCEEEEEE
QDVRGQGTSDGIFTEFGNDGDDGYDTVEWAAGLPGATGKVGMYGSSYVGATQWLAATTTP
EECCCCCCCCCEEEECCCCCCCCCCHHHHHCCCCCCCCCEECCCCCCCCHHHEEEECCCC
PHLTTIVPSNTASDYYDGWTYEGGAFRLGFVQPWAMGTIATTAAKNRADLAALEQLRAAA
CCEEEEECCCCCCCCCCCEEECCCCEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHHH
AERGRWLNHLPYQDLPPMQPGDPRVAPWYFDWIRHSTRDDYWKRWSIRDRYQNVTVPVLH
HHHCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHCCCHHHHHCCCEEEEEE
FEGWYDAFLRGGLENFAGMTARGGSPGARRYQRMVIGPWDHLAWGRESSSPSPLLKGLGP
ECHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHEEEECCHHHHCCCCCCCCCCHHHHHCCC
GANSPVNEMQLAWFDHFLKGSDNGVATESPRLDYYLMGANRWKHASAWPLPQTNWTVYHL
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEC
GGRGTQHNGTLRDSAAAHDAPDVYLYDPANPVPSAGGHSCCAAESAPQGRYDQRAVEQRP
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHCC
DVLTYTTEPLSTNTEITGPISVDLWASSSAPDTDFTAKLVAVQADGTAANLNNGIVRTSL
CEEEEECCCCCCCCEECCCEEEEEEECCCCCCCCCEEEEEEEECCCCEECCCCCCHHHHH
AQSLSERRLMPIGQPHKYHIDIWPVSYQFRTGERIRLEISSSDFPQYAPNPNTAAPFGRN
HHHHHHCCCCCCCCCCEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
AELRTATQIILHDAAHPSTLTLPVIPAGDPGTETFPMPRRPDDPPGN
CCHHHHEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]