Click here to switch to the map view.

The map label for this gene is nylA [H]

Identifier: 169629267

GI number: 169629267

Start: 2194143

End: 2195618

Strand: Reverse

Name: nylA [H]

Synonym: MAB_2181c

Alternate gene names: 169629267

Gene position: 2195618-2194143 (Counterclockwise)

Preceding gene: 169629268

Following gene: 169629266

Centisome position: 43.33

GC content: 66.53

Gene sequence:

>1476_bases
GTGGATTTCTCCGACTACTCCCAACATGACGCCACCGGCCTGGCCGAGCTGGTCGCCACCAAACAGGTCACCCCAGCCGA
GCTGCTGGCCGCGGCCCGCGCTCGCGCTGCCGACGTCAACAACAAGATCGGTGCGATAGTCCGAACTCTCCCCGAAGCCG
ACCAGCAAGTGCAGGGCGACCTGACCGGGCCGTTCGCCGGGGTGCCGTTTCTCATCAAGGACCTCGGCCAGGACTACAAG
GGCCATCCCACATCGGGAGGATGCCGGGCGTTGTCCACACTCCCCGCGCCGGAGCACTCCACCATCGTGCGACGCTGGCT
CGATGCCGGCCTGGTCATCTTCGGCAAGACCAACACCCCGGAGTTCGGGTCCAAGGGCATCACCGAGCCGGAATTGTTTG
GGGCGAGCCGTAACCCGTGGGACCTAGGGCGCACACCCGGCGGCTCCTCGGGCGGCTCGGCCGCCGCGGTCGCCGCCGGA
ATCGTGCCGGTGGCGGGAGCCAGCGATGGCGGCGGATCGATCCGGATCCCCGCCGGATGTTGCGGCCTGGTTGGGCTCAA
GCCCGGCCGCGGTATCACCCCGATGGGTCCCACAGCCGGCGAGCCGATGCATGGCGCGGCGGTCAACGGCACCGTCTCCC
GAACCGTCCGCGATACCGCGTCCATGCTCGATATCATCGCCGGGCCCGAGCCCACCTCGCCGTATTTGTCTGCGATGCCC
CAGGATTCGTACGCATCGCAAGTAGGACGTGATCCTGGCAGGCTCCGTATCGGGGTGCGCGTGCCAACCGTGATCACTCC
GAACCCCGATCCCGCCGCGGTGGCCGCGGTCGAGACCGCCATCCGTCTGCTGACCCATCTGGGGCACGAGGTAGAGGAGG
TGAGCGCACCCTATGACGATGGTCAGCTCGCCCGCGACTTCTTGTTGACGTGGTTCGTGCATGCCGCCTACGAGGTGGAC
TACACCAAGCGGCTGACCGGAGCGGGAGACGAGGGGTTTGAGCGCGACACGCTCCTCATCGCGGCATTAGGACGCACCAC
CAGCGGACCCGAATATGTTGCGGCCGTGGAACGCCGACACGACTACGTACGGGAGCTGGCGACCTTCTTCGGCGACTACG
ATCTGCTGCTCACGCCGACACTCGCGAAGGCGCCGCCGCGGATCGGCGAGTTCAATCTCCCCGCGCCACTCGCCGCCGTG
TCTGATCTGTTGCTGCGCACCAAGACCGCCCGCGTCCTCAAATACGCGAACATTGTCGACAGCATGATCAACGACAACCT
CGGCTGGGTCCCCTACACCCAGTTGGCGAATCTGACAGGGCGCCCAGCGATCTCATTGCCGCTCTATCAGACACCGAAGG
GGCTGCCCCTCGGTGTGCAGTTTGTCGCACCGCTCGGCGGCGAATCACTCCTGATTCGGCTGGCTGCCCAGCTCGAGGCA
ACCGAACCGTGGGCACACCGGCGCCCAACCCTATAG

Upstream 100 bases:

>100_bases
GCCGCGGCAGTCGCTCCAGACCGAGAAGATCGTCGCGATCTACAACGACGTGTTGAAGAAGGCCAACGCTTGACCCGGTG
AGGCCGCATAGGCTGATCGG

Downstream 100 bases:

>100_bases
CGCGGTAATGTGGACGCCGGGCGTGCAGGAGCGCCCGGTGTCCGCCCGAAGAGGCTGTGTGCCGATCCCACGATCACCGA
CTTACGGTATGGGACAACAC

Product: putative amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MDFSDYSQHDATGLAELVATKQVTPAELLAAARARAADVNNKIGAIVRTLPEADQQVQGDLTGPFAGVPFLIKDLGQDYK
GHPTSGGCRALSTLPAPEHSTIVRRWLDAGLVIFGKTNTPEFGSKGITEPELFGASRNPWDLGRTPGGSSGGSAAAVAAG
IVPVAGASDGGGSIRIPAGCCGLVGLKPGRGITPMGPTAGEPMHGAAVNGTVSRTVRDTASMLDIIAGPEPTSPYLSAMP
QDSYASQVGRDPGRLRIGVRVPTVITPNPDPAAVAAVETAIRLLTHLGHEVEEVSAPYDDGQLARDFLLTWFVHAAYEVD
YTKRLTGAGDEGFERDTLLIAALGRTTSGPEYVAAVERRHDYVRELATFFGDYDLLLTPTLAKAPPRIGEFNLPAPLAAV
SDLLLRTKTARVLKYANIVDSMINDNLGWVPYTQLANLTGRPAISLPLYQTPKGLPLGVQFVAPLGGESLLIRLAAQLEA
TEPWAHRRPTL

Sequences:

>Translated_491_residues
MDFSDYSQHDATGLAELVATKQVTPAELLAAARARAADVNNKIGAIVRTLPEADQQVQGDLTGPFAGVPFLIKDLGQDYK
GHPTSGGCRALSTLPAPEHSTIVRRWLDAGLVIFGKTNTPEFGSKGITEPELFGASRNPWDLGRTPGGSSGGSAAAVAAG
IVPVAGASDGGGSIRIPAGCCGLVGLKPGRGITPMGPTAGEPMHGAAVNGTVSRTVRDTASMLDIIAGPEPTSPYLSAMP
QDSYASQVGRDPGRLRIGVRVPTVITPNPDPAAVAAVETAIRLLTHLGHEVEEVSAPYDDGQLARDFLLTWFVHAAYEVD
YTKRLTGAGDEGFERDTLLIAALGRTTSGPEYVAAVERRHDYVRELATFFGDYDLLLTPTLAKAPPRIGEFNLPAPLAAV
SDLLLRTKTARVLKYANIVDSMINDNLGWVPYTQLANLTGRPAISLPLYQTPKGLPLGVQFVAPLGGESLLIRLAAQLEA
TEPWAHRRPTL
>Mature_491_residues
MDFSDYSQHDATGLAELVATKQVTPAELLAAARARAADVNNKIGAIVRTLPEADQQVQGDLTGPFAGVPFLIKDLGQDYK
GHPTSGGCRALSTLPAPEHSTIVRRWLDAGLVIFGKTNTPEFGSKGITEPELFGASRNPWDLGRTPGGSSGGSAAAVAAG
IVPVAGASDGGGSIRIPAGCCGLVGLKPGRGITPMGPTAGEPMHGAAVNGTVSRTVRDTASMLDIIAGPEPTSPYLSAMP
QDSYASQVGRDPGRLRIGVRVPTVITPNPDPAAVAAVETAIRLLTHLGHEVEEVSAPYDDGQLARDFLLTWFVHAAYEVD
YTKRLTGAGDEGFERDTLLIAALGRTTSGPEYVAAVERRHDYVRELATFFGDYDLLLTPTLAKAPPRIGEFNLPAPLAAV
SDLLLRTKTARVLKYANIVDSMINDNLGWVPYTQLANLTGRPAISLPLYQTPKGLPLGVQFVAPLGGESLLIRLAAQLEA
TEPWAHRRPTL

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=235, Percent_Identity=33.1914893617021, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI222831590, Length=523, Percent_Identity=23.9005736137667, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI166795287, Length=171, Percent_Identity=33.9181286549708, Blast_Score=67, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17556264, Length=302, Percent_Identity=31.7880794701987, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17556276, Length=307, Percent_Identity=30.9446254071661, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17556278, Length=307, Percent_Identity=30.9446254071661, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17537465, Length=244, Percent_Identity=28.6885245901639, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=300, Percent_Identity=28, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=293, Percent_Identity=27.6450511945392, Blast_Score=79, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=160, Percent_Identity=35.625, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=203, Percent_Identity=28.0788177339901, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21356731, Length=500, Percent_Identity=26.4, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24648113, Length=480, Percent_Identity=30.2083333333333, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI161078093, Length=513, Percent_Identity=25.3411306042885, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652985, Length=529, Percent_Identity=24.5746691871456, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922090, Length=529, Percent_Identity=24.5746691871456, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24652981, Length=529, Percent_Identity=24.5746691871456, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24652983, Length=529, Percent_Identity=24.5746691871456, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24644968, Length=240, Percent_Identity=30.4166666666667, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI45550774, Length=248, Percent_Identity=30.6451612903226, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648435, Length=248, Percent_Identity=30.6451612903226, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648437, Length=248, Percent_Identity=30.6451612903226, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648441, Length=178, Percent_Identity=32.0224719101124, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24648439, Length=178, Percent_Identity=32.0224719101124, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 51768; Mature: 51768

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFSDYSQHDATGLAELVATKQVTPAELLAAARARAADVNNKIGAIVRTLPEADQQVQGD
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHCCC
LTGPFAGVPFLIKDLGQDYKGHPTSGGCRALSTLPAPEHSTIVRRWLDAGLVIFGKTNTP
CCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCC
EFGSKGITEPELFGASRNPWDLGRTPGGSSGGSAAAVAAGIVPVAGASDGGGSIRIPAGC
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEECCCCCCCCEEEECCCC
CGLVGLKPGRGITPMGPTAGEPMHGAAVNGTVSRTVRDTASMLDIIAGPEPTSPYLSAMP
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCC
QDSYASQVGRDPGRLRIGVRVPTVITPNPDPAAVAAVETAIRLLTHLGHEVEEVSAPYDD
CHHHHHHHCCCCCEEEEEEEECEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCC
GQLARDFLLTWFVHAAYEVDYTKRLTGAGDEGFERDTLLIAALGRTTSGPEYVAAVERRH
HHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHH
DYVRELATFFGDYDLLLTPTLAKAPPRIGEFNLPAPLAAVSDLLLRTKTARVLKYANIVD
HHHHHHHHHHCCCCEEECCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SMINDNLGWVPYTQLANLTGRPAISLPLYQTPKGLPLGVQFVAPLGGESLLIRLAAQLEA
HHHCCCCCCCCHHHHHCCCCCCEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCC
TEPWAHRRPTL
CCCCCCCCCCC
>Mature Secondary Structure
MDFSDYSQHDATGLAELVATKQVTPAELLAAARARAADVNNKIGAIVRTLPEADQQVQGD
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHCCC
LTGPFAGVPFLIKDLGQDYKGHPTSGGCRALSTLPAPEHSTIVRRWLDAGLVIFGKTNTP
CCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCC
EFGSKGITEPELFGASRNPWDLGRTPGGSSGGSAAAVAAGIVPVAGASDGGGSIRIPAGC
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEECCCCCCCCEEEECCCC
CGLVGLKPGRGITPMGPTAGEPMHGAAVNGTVSRTVRDTASMLDIIAGPEPTSPYLSAMP
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCC
QDSYASQVGRDPGRLRIGVRVPTVITPNPDPAAVAAVETAIRLLTHLGHEVEEVSAPYDD
CHHHHHHHCCCCCEEEEEEEECEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCC
GQLARDFLLTWFVHAAYEVDYTKRLTGAGDEGFERDTLLIAALGRTTSGPEYVAAVERRH
HHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHH
DYVRELATFFGDYDLLLTPTLAKAPPRIGEFNLPAPLAAVSDLLLRTKTARVLKYANIVD
HHHHHHHHHHCCCCEEECCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SMINDNLGWVPYTQLANLTGRPAISLPLYQTPKGLPLGVQFVAPLGGESLLIRLAAQLEA
HHHCCCCCCCCHHHHHCCCCCCEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCC
TEPWAHRRPTL
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]