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The map label for this gene is nuoE [H]

Identifier: 169629224

GI number: 169629224

Start: 2149087

End: 2149830

Strand: Direct

Name: nuoE [H]

Synonym: MAB_2138

Alternate gene names: 169629224

Gene position: 2149087-2149830 (Clockwise)

Preceding gene: 169629223

Following gene: 169629225

Centisome position: 42.41

GC content: 63.04

Gene sequence:

>744_bases
GTGACCGAGGTATTTGTCGAACTGGGTAGCCGTCCGTCAGAGGACGAAGGTCGATTCGCTGGGCGTAAACACTATCCGGC
GGAGGTGGTTTCACGGCTGGCCATCGACGCCAAGGAGATCCTCGATCGCTATCCAAGCCGTCGGTCGGCATTGCTGCCGC
TGCTGCATCTGGTCCAGTCCGAAGACGGATACGTGACCACGGCAGGCATCGAATTCTGTGCCCGACAAGTGGAATTGACA
TCCGCCGAGGTGACCGCAGTGGCGAGTTTCTATTCGATGTACCGGCGCGAACCCACCGGTGACTACCTCGTGGGTGTCTG
TACCAATACGTTGTGCGCGGTGCTCGGCGGCGACGCTATTCTGGCGCGATTGGCCGGGGAACTCGGTGTGCGTCCGGGCG
GCACCACCGAGGACGGCAAGGTCACCCTCGAACACATCGAGTGTAACGCCGGCTGTGACTACGCGCCGGTCCTGATGGTC
AATTGGGAGTTCTTCGATAACCAAACCCCGGATGGGGCAGCGGCTCTGGTCCAAGGGCTGCGGCGTGGTCATGTTCCCGC
GCCCAGACGAGGCGCGCCACCGTGCACATTCAAGGAGACGGCCCGGATCTTGGCGGGGTTCGCCGATGAGCGGCCGGAAG
CGGTCGCCGCAGCACAGCCGGGGGACTCGACACTTGCGGGACTGCGGTTGGCCCAGGACGTACAGCGGAACGTTCAGTAC
AAGGCAGACGGGATGAGGCCATGA

Upstream 100 bases:

>100_bases
CGAACCTGCAGTCCGTTGCCGCCATGTGTGAGGGCGCGATGGTGGCCGACTTGATCGCCGCGGTGGCGAGCATTGATCCG
GTGATGGGGGGAGTGGACAG

Downstream 100 bases:

>100_bases
CTTCGTCGATTCTCTCTCCGGTCCTGTCGGAGCATTGGGACGAGCCTGATGCCTGGACCTTGGATGGGTATCTGCGTCAT
GGCGGATACCGCGGCCTGCG

Product: NADH-quinone oxidoreductase, E subunit NuoE

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MTEVFVELGSRPSEDEGRFAGRKHYPAEVVSRLAIDAKEILDRYPSRRSALLPLLHLVQSEDGYVTTAGIEFCARQVELT
SAEVTAVASFYSMYRREPTGDYLVGVCTNTLCAVLGGDAILARLAGELGVRPGGTTEDGKVTLEHIECNAGCDYAPVLMV
NWEFFDNQTPDGAAALVQGLRRGHVPAPRRGAPPCTFKETARILAGFADERPEAVAAAQPGDSTLAGLRLAQDVQRNVQY
KADGMRP

Sequences:

>Translated_247_residues
MTEVFVELGSRPSEDEGRFAGRKHYPAEVVSRLAIDAKEILDRYPSRRSALLPLLHLVQSEDGYVTTAGIEFCARQVELT
SAEVTAVASFYSMYRREPTGDYLVGVCTNTLCAVLGGDAILARLAGELGVRPGGTTEDGKVTLEHIECNAGCDYAPVLMV
NWEFFDNQTPDGAAALVQGLRRGHVPAPRRGAPPCTFKETARILAGFADERPEAVAAAQPGDSTLAGLRLAQDVQRNVQY
KADGMRP
>Mature_246_residues
TEVFVELGSRPSEDEGRFAGRKHYPAEVVSRLAIDAKEILDRYPSRRSALLPLLHLVQSEDGYVTTAGIEFCARQVELTS
AEVTAVASFYSMYRREPTGDYLVGVCTNTLCAVLGGDAILARLAGELGVRPGGTTEDGKVTLEHIECNAGCDYAPVLMVN
WEFFDNQTPDGAAALVQGLRRGHVPAPRRGAPPCTFKETARILAGFADERPEAVAAAQPGDSTLAGLRLAQDVQRNVQYK
ADGMRP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton transloca

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=151, Percent_Identity=32.4503311258278, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1788621, Length=138, Percent_Identity=30.4347826086957, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17561328, Length=158, Percent_Identity=34.8101265822785, Blast_Score=110, Evalue=6e-25,
Organism=Drosophila melanogaster, GI18859877, Length=172, Percent_Identity=32.5581395348837, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI21355353, Length=158, Percent_Identity=33.5443037974684, Blast_Score=98, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 26683; Mature: 26552

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVFVELGSRPSEDEGRFAGRKHYPAEVVSRLAIDAKEILDRYPSRRSALLPLLHLVQS
CCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHC
EDGYVTTAGIEFCARQVELTSAEVTAVASFYSMYRREPTGDYLVGVCTNTLCAVLGGDAI
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCHHH
LARLAGELGVRPGGTTEDGKVTLEHIECNAGCDYAPVLMVNWEFFDNQTPDGAAALVQGL
HHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEEEEEECCCCCCCHHHHHHHHH
RRGHVPAPRRGAPPCTFKETARILAGFADERPEAVAAAQPGDSTLAGLRLAQDVQRNVQY
HHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCEE
KADGMRP
CCCCCCC
>Mature Secondary Structure 
TEVFVELGSRPSEDEGRFAGRKHYPAEVVSRLAIDAKEILDRYPSRRSALLPLLHLVQS
CCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHC
EDGYVTTAGIEFCARQVELTSAEVTAVASFYSMYRREPTGDYLVGVCTNTLCAVLGGDAI
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCHHH
LARLAGELGVRPGGTTEDGKVTLEHIECNAGCDYAPVLMVNWEFFDNQTPDGAAALVQGL
HHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEEEEEECCCCCCCHHHHHHHHH
RRGHVPAPRRGAPPCTFKETARILAGFADERPEAVAAAQPGDSTLAGLRLAQDVQRNVQY
HHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCEE
KADGMRP
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]